公开(公告)号：US11014129B2

公开(公告)日：2021-05-25

申请号：US14912440

申请日：2014-06-19

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Sapna Mehtani ,  John Dana Hubbard

IPC: B08B9/027 ,  C07K1/22 ,  A61L2/18 ,  B08B3/08

Abstract: The present invention provides methods for cleaning a Protein A chromatography column employing a media comprising a Protein A ligand derived from the C domain of Staphylococcus aureus, such that the column can be cleaned using both acidic and alkaline solutions.

公开(公告)号：US20200330981A1

公开(公告)日：2020-10-22

申请号：US16607743

申请日：2018-05-10

Applicant: EMD Millipore Corporation

Inventor： Phillip Clark ,  Kurt E. Greenizen ,  John Doyle

IPC: B01L3/00 ,  C07K1/34 ,  B01D61/58 ,  B01D61/18

Abstract: Filtration apparatus for the assay of biological and biochemical reactants, for example, is provided and includes a substrate such as a plate having one or more wells open at each end, and a porous membrane positioned in each well forming a discrete filtering area. The filtration apparatus includes a seal that is in a compressible relationship with the face of the porous membrane, the surface of the compression element, and the well wall. Each well includes a compression element, such as an internal well insert or sleeve, which compresses the seal so that the seal contacts the membrane face, the surface of the compression element, and the well wall in a liquid-tight manner. The compression element may be configured so that it is fixed in the well such as by an interference fit with the well wall or by bonding to a surface of the substrate.

公开(公告)号：US10793593B2

公开(公告)日：2020-10-06

申请号：US13732613

申请日：2013-01-02

Applicant: EMD Millipore Corporation

Inventor： Wilson Moya

IPC: C07K1/36 ,  C07K1/32 ,  C07K1/30 ,  C07K16/00

Abstract: The present invention relates to a selectively soluble polymer capable of binding to one or more constituents in a mixture containing various biological materials and the methods of using such a polymer to purify a biomolecule from such a mixture. The polymer is soluble in the mixture under a certain set of process conditions such as pH or temperature and is rendered insoluble and precipitates out of solution upon a change in the process conditions. While in its solubilized state, the polymer is capable of binding to a selected entity within the stream such as impurities (DNA, RNA, host cell protein, endotoxins, etc.) in a cell broth and remains capable of binding to that entity even after the polymer is precipitated out of solution. The precipitate can then be filtered out from the remainder of the stream and the desired biomolecule is recovered and further processed.

公开(公告)号：US20200276587A1

公开(公告)日：2020-09-03

申请号：US16878522

申请日：2020-05-19

Applicant: EMD Millipore Corporation

Inventor： Joseph Muldoon ,  Rene Reinbigler

IPC: B01L3/00 ,  G01N1/14 ,  A61B5/15

Abstract: A fluid transfer device includes a syringe barrel having a chamber, a first plunger slidably movable inside the chamber, and a second plunger slidably movable inside the chamber. The distal end portion of the first plunger is engageable with the proximal end portion of the second plunger such that when the distal end portion of the first plunger and the proximal end portion of the second plunger are engaged, the second plunger is movable by the first plunger. A check valve may be incorporated into the distal end portion of the second plunger to allow a fluid to pass therethrough in a direction towards the proximal end portion of the second plunger and prevent a fluid to pass therethrough in a reverse direction. A fluid transfer assembly and a sampling method are also described.

公开(公告)号：US20200208089A1

公开(公告)日：2020-07-02

申请号：US16713117

申请日：2019-12-13

Applicant: EMD Millipore Corporation

Inventor： Paul J. Hung ,  Philip J. Lee

IPC: C12M1/32 ,  C12M3/06 ,  C12M1/00 ,  C12M1/12

Abstract: Microfluidic devices, systems, and methods providing for an invasion assay using microfluidic culture systems.

公开(公告)号：US20200172568A1

公开(公告)日：2020-06-04

申请号：US16610616

申请日：2018-04-18

Applicant: EMD Millipore Corporation

Inventor： Ronald Tuccelli ,  John Caulmare ,  Luc Messier ,  Melissa Holstein ,  Christopher Gillespie ,  Roger Hill

IPC: C07K1/22 ,  B01L3/00 ,  C12N7/00 ,  A61L2/00 ,  A61L2/26 ,  B01D15/38 ,  B01D15/36 ,  B01D15/24

Abstract: Methods of maintaining narrow residence time distributions in continuous flow systems, particularly applicable to virus inactivation such as during a protein purification process. Fluid sample is introduced into an axial flow channel and caused to flow therein in discrete packets or zones to minimize residence time distribution and axial dispersion. Embodiments described herein obviate or minimize the need for using large tanks or reservoirs for performing virus inactivation during a protein purification process; reduce the overall time required for virus inactivation, and/or reduce the overall physical space required to perform the virus inactivation operation during a protein purification process, which in turn reduces the overall footprint for the purification process.

公开(公告)号：US20190218250A1

公开(公告)日：2019-07-18

申请号：US16358845

申请日：2019-03-20

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Christopher Gillespie ,  Matthew T. Stone ,  Mikhail Kozlov ,  Jie Chen ,  Martin Siwak

IPC: C07K1/36 ,  B01J20/20 ,  B01D15/12 ,  B01D15/36 ,  C07K16/00 ,  B01J47/04 ,  B01J43/00 ,  B01J41/20 ,  B01J39/26 ,  C07K1/20 ,  C07K1/16 ,  B01J20/282

CPC classification number: C07K1/36 ,  B01D15/125 ,  B01D15/1871 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3809 ,  B01J20/20 ,  B01J20/282 ,  B01J39/26 ,  B01J41/20 ,  B01J43/00 ,  B01J47/04 ,  C07K1/16 ,  C07K1/20 ,  C07K16/00 ,  C07K2317/10

Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely affecting the yield of the desired protein product.

公开(公告)号：US10350518B2

公开(公告)日：2019-07-16

申请号：US14839852

申请日：2015-08-28

Applicant: EMD Millipore Corporation

Inventor： Jonathan Steen ,  Mikhail Kozlov ,  Herbert Lutz ,  Joseph Parrella ,  Elizabeth M. Goodrich ,  Bala Raghunath

IPC: B01D29/60 ,  B01D29/52 ,  B01D29/90 ,  B01D61/14 ,  B01D63/04 ,  B01D63/12

Abstract: Methods of filtering a liquid feed are disclosed. In one version, the method comprises passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers without recirculation through the SPTFF system. In another version, the method of filtering a liquid feed, comprises passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

公开(公告)号：US20190099702A1

公开(公告)日：2019-04-04

申请号：US16208018

申请日：2018-12-03

Applicant: EMD Millipore Corporation

Inventor： Jonathan Steen ,  Mikhail Kozlov ,  Herbert Lutz ,  Joseph Parrella ,  Elizabeth M. Goodrich ,  Bala Raghunath

IPC: B01D29/90 ,  B01D29/52 ,  B01D61/14 ,  B01D29/60 ,  B01D63/08

Abstract: A method of filtering a liquid feed is described, comprising passing a liquid feed through a single pass tangential flow filtration (SPTFF) system and recovering the retentate and permeate from the system in separate containers. A method of filtering a liquid feed is also described comprising passing a liquid feed through a tangential flow filtration (TFF) system, recovering permeate and a portion of the retentate from the system in separate containers without recirculation through the TFF system, and recirculating the remainder of the retentate through the TFF system at least once. The methods of the invention can be performed using an SPTFF or a TFF system that comprises manifold segments to serialize the flow path of the feed and retentate without requiring diverter plates.

公开(公告)号：US10207225B2

公开(公告)日：2019-02-19

申请号：US14736646

申请日：2015-06-11

Applicant: EMD Millipore Corporation

Inventor： Herbert Lutz ,  Joseph Parrella ,  Bala Raghunath

IPC: C07K1/34 ,  B01D61/14 ,  B01D61/18 ,  B01D63/08 ,  B01D65/02

Abstract: The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly.