公开(公告)号：US20250019731A1

公开(公告)日：2025-01-16

申请号：US18595947

申请日：2024-03-05

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Haibo WANG ,  Stephen B. DEL CARDAYRE ,  Zhihao HU ,  Louis G. HOM ,  Baolong ZHU ,  Cindy CHANG ,  Emanuela E. POPOVA

IPC: C12P7/6409 ,  C12N9/02 ,  C12N9/04 ,  C12N15/52 ,  C12N15/62 ,  C12P7/18 ,  C12P7/42 ,  C12P7/6436

Abstract: The disclosure relates to omega-hydroxylated fatty acid derivatives and methods of producing them. Herein, the disclosure encompasses a novel and environmentally friendly production method that provides omega-hydroxylated fatty acid derivatives at high purity and yield. Further encompassed are recombinant microorganisms that produce omega-hydroxylated fatty acid derivatives through selective fermentation.

公开(公告)号：US20240309405A1

公开(公告)日：2024-09-19

申请号：US18339397

申请日：2023-06-22

Applicant: Genomatica, Inc.

Inventor： Andreas W. SCHIRMER ,  Risha Lindig Bond ,  Erin Frances Perry

IPC: C12P7/18 ,  C07C68/00 ,  C07C69/22 ,  C07C69/30 ,  C12N15/52

CPC classification number: C12P7/18 ,  C07C68/00 ,  C07C69/22 ,  C07C69/30 ,  C12N15/52

Abstract: The disclosure relates to the field of specialty chemicals and methods for their synthesis. In embodiments, the disclosure provides novel multifunctional fatty acid derivative molecules such as e.g., fatty triols, fatty tetrols, dihydroxy fatty acids, etc. The disclosure further provides derivatives of the disclosed multifunctional molecules which are useful e.g., in the production of personal care products, surfactants, detergents, polymers, paints, coatings, and as emulsifiers, emollients, and thickeners in cosmetics and foods, as industrial solvents and plasticizers, etc. The disclosure further provides biochemical pathways, recombinant microorganisms and methods for the biological production of various multifuctional fatty acid derivatives.

公开(公告)号：US20240229086A1

公开(公告)日：2024-07-11

申请号：US18284349

申请日：2022-03-22

Applicant: GENOMATICA, INC.

Inventor： Andreas W. SCHIRMER ,  Erin Frances PERRY ,  Alma Itzel RAMOS-SOLIS ,  Angelica ZABALA-BAUTISTA

IPC: C12P7/6409 ,  C12N1/20 ,  C12N9/02 ,  C12N9/04 ,  C12N9/10 ,  C12N9/12 ,  C12N9/16 ,  C12N9/88

CPC classification number: C12P7/6409 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/1288 ,  C12N9/16 ,  C12N9/88 ,  C12Y114/19002 ,  C12Y203/01084 ,  C12Y207/08007 ,  C12Y301/02014 ,  C12Y402/01059

Abstract: Recombinant proteobacteria, including γ-proteobacteria, comprising a heterologous acyl-ACP desaturase and a heterologous acyl-ACP thioesterase, wherein the native dual 3-hydroxy acyl-ACP dehydratase/isomerase is deleted are provided herein. The recombinant proteobacteria produce non-native monounsaturated free fatty acids or derivatives thereof. Methods of producing non-native monounsaturated free fatty acids or derivatives thereof are also provided, in addition to cell cultures and fatty acid compositions produced by the recombinant proteobacteria. The recombinant proteobacteria may be used to produce insect pheromones or precursors thereof, and fragrances or precursors thereof.

公开(公告)号：US20240117324A1

公开(公告)日：2024-04-11

申请号：US18355990

申请日：2023-07-20

Applicant: Genomatica, Inc.

Inventor： Mathew RUDE ,  Na Trinh ,  Andreas Schirmer ,  Jacob Gano

IPC: C12N9/02 ,  C12P7/04 ,  C12P7/24 ,  C12P7/6409

CPC classification number: C12N9/0008 ,  C12P7/04 ,  C12P7/24 ,  C12P7/6409 ,  C12Y102/0108

Abstract: The disclosure relates to acyl-ACP reductase (AAR) enzyme variants that result in improved fatty aldehyde and fatty alcohol production when expressed in recombinant host cells. The disclosure further relates to methods of making and using such AAR variants for the production of fatty alcohol compositions having particular characteristics.

公开(公告)号：US11834690B2

公开(公告)日：2023-12-05

申请号：US17527902

申请日：2021-11-16

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Anthony P. Burgard ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/00 ,  C12P13/02 ,  C12P17/12

CPC classification number: C12P17/10 ,  C12N1/38 ,  C12N15/52 ,  C12P7/40 ,  C12P7/44 ,  C12P13/001 ,  C12P13/005 ,  C12P13/02 ,  C12P17/12

Abstract: The invention provides a non-naturally occurring microbial organism having a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in the respective 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway. The invention additionally provides a method for producing 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid. The method can include culturing a 6-aminocaproic acid, caprolactam or hexametheylenediamine producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid pathway enzyme in a sufficient amount to produce the respective product, under conditions and for a sufficient period of time to produce 6-aminocaproic acid, caprolactam, hexametheylenediamine or levulinic acid.

公开(公告)号：US20230332194A1

公开(公告)日：2023-10-19

申请号：US18158680

申请日：2023-01-24

Applicant: Genomatica, Inc.

Inventor： Anthony P. BURGARD ,  Robin E. OSTERHOUT ,  Stephen J. VAN DIEN ,  Cara Ann TRACEWELL ,  Priti PHARKYA ,  Stefan ANDRAE

IPC: C12P17/10 ,  C12P7/44 ,  C12P7/24 ,  C12P13/00 ,  C12N9/04 ,  C08G63/78 ,  C08G73/02 ,  C08G69/16 ,  C08G69/08 ,  A23L29/00 ,  C12N15/52

CPC classification number: C12P17/10 ,  C12P7/44 ,  C12P7/24 ,  C12P13/001 ,  C12N9/0006 ,  C08G63/78 ,  C08G73/0213 ,  C08G69/16 ,  C08G69/08 ,  A23L29/065 ,  C12N15/52 ,  C12P13/005 ,  C12Y101/01244

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.

公开(公告)号：US20230265397A1

公开(公告)日：2023-08-24

申请号：US17943482

申请日：2022-09-13

Applicant: Genomatica, Inc.

Inventor： Nelson R. Barton ,  Jingyi Li ,  Joseph R. Warner ,  Priti Pharkya

IPC: C12N9/04 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N9/10 ,  C12P7/24

CPC classification number: C12N9/0006 ,  C12N9/88 ,  C12N9/90 ,  C12Y401/02043 ,  C12Y503/01027 ,  C12N15/52 ,  C12N9/1029 ,  C12P7/24 ,  C12Y101/02007 ,  C12Y203/01085 ,  C12Y401/02013 ,  C07K2319/00

Abstract: Described herein are fusion proteins including methanol dehydrogenase (MeDH) and at least one other polypeptide such as 3-hexulose-6-phosphate dehydrogenase (HPS) or 6-phospho-3-hexuloisomerase (PHI), such as DHAS synthase or fructose-6-Phosphate aldolase or such as DHA synthase or DHA kinase. In a localized manner, the fusion protein can promote the conversion of methanol to formaldehyde and then to a ketose phosphate such as hexulose 6-phosphate or then to DHA and G3P. When expressed in cells, the fusion proteins can promote methanol uptake and rapid conversion to the ketose phosphate or to the DHA and D3P, which in turn can be used in a pathway for the production of a desired bioproduct. Beneficially, the rapid conversion to the ketose phosphate or to the DHA and G3P can avoid the undesirable accumulation of formaldehyde in the cell. Also described are engineered cells expressing the fusion protein, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

公开(公告)号：US20230167419A1

公开(公告)日：2023-06-01

申请号：US17818038

申请日：2022-08-08

Applicant: Genomatica, Inc.

Inventor： Baolong ZHU ,  Andreas W. Schirmer ,  Cindy Chang

IPC: C12N9/02 ,  C12P7/6409

CPC classification number: C12N9/0077 ,  C12P7/6409

Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for ex pressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.

公开(公告)号：US20230159902A1

公开(公告)日：2023-05-25

申请号：US17861036

申请日：2022-07-08

Applicant: Genomatica, Inc.

Inventor： Stefan ANDRAE ,  Michael Patrick KUCHINSKAS ,  Jingyi LI ,  Harish NAGARAJAN ,  Priti PHARKYA

IPC: C12N9/04 ,  C12P7/24 ,  C12P7/18

CPC classification number: C12N9/0006 ,  C12P7/24 ,  C12Y101/01244 ,  C12P7/18 ,  Y02P20/10 ,  C12Y101/01001

Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

公开(公告)号：US11629363B2

公开(公告)日：2023-04-18

申请号：US15873798

申请日：2018-01-17

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12N1/14 ,  C12N1/16 ,  C12N1/20 ,  C12P7/18 ,  C12P7/20 ,  C12P7/24 ,  C12N9/04 ,  C12N9/06 ,  C12N9/10 ,  C12N15/52 ,  C12P7/04

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol. Also provided herein are methods for using such an organism to produce 1,2-propanediol, n-propanol, 1,3-propanediol or glycerol.