公开(公告)号：US11982603B2

公开(公告)日：2024-05-14

申请号：US17702276

申请日：2022-03-23

申请人： Bruker Daltonics GmbH & Co. KG

发明人： Katrin Sparbier ,  Beatrix Wegemann

IPC分类号： G01N1/40 ,  C12Q1/04 ,  C12Q1/18 ,  G01N1/28 ,  G01N33/68

CPC分类号： G01N1/4044 ,  C12Q1/045 ,  C12Q1/18 ,  G01N1/28 ,  G01N33/6851 ,  G01N1/4055

摘要： The invention relates to the preparation of biological cells for the mass spectrometric analysis of cellular properties such as taxonomic classification, antibiotic resistances, response to drugs or other active substances, and others. The cells can be prokaryotic or eukaryotic microorganisms which have particularly been cultivated directly on a mass spectrometric sample support, or eukaryotic cells from tissues or cell cultures. The invention proposes that the cells are not disrupted by adding matrix solution for a subsequent ionization by matrix-assisted laser desorption (MALDI), but that they are disrupted in a separate treatment step using acids and/or solvents on the sample support itself. Surprisingly, the cell proteins released then adhere to the sample support so that they can be carefully washed with buffer solution to remove salts and other soluble impurities which can stem from earlier treatment steps, for example from nutrient solution.

公开(公告)号：US20240102978A1

公开(公告)日：2024-03-28

申请号：US17996103

申请日：2021-04-12

申请人： GLAXOSMITHKLINE BIOLOGICALS SA

发明人： Carlo Giannelli ,  Francesca Micoli ,  Maria Michelina Raso

IPC分类号： G01N30/88 ,  G01N1/40 ,  G01N30/06

CPC分类号： G01N30/88 ,  G01N1/4044 ,  G01N30/06 ,  G01N2001/4016 ,  G01N2030/8836 ,  G01N2333/255

摘要： The present invention provides a method for measuring the concentration and/or amount of one or more polysaccharide in a test sample comprising or consisting of the steps of (a) acid hydrolysis of the test sample with hydrochloric acid and trifluoroaceticacid; (b) chromatographic separation of the hydrolysed test sample of step (a); and (c) determining the concentration and/or amount of the one or more polysaccharide based on the data generated in step (b), together with processed test samples and kit for use of the same.

公开(公告)号：US20240068995A1

公开(公告)日：2024-02-29

申请号：US18365467

申请日：2023-08-04

申请人： Waters Technologies Corporation

发明人： Robert Birdsall ,  Ying Qing Yu ,  Jacob Kellett

IPC分类号： G01N30/08 ,  C12Q1/37 ,  G01N1/40 ,  G01N30/72 ,  G01N30/74 ,  G01N33/15 ,  G01N33/68

CPC分类号： G01N30/08 ,  C12Q1/37 ,  G01N1/4044 ,  G01N1/405 ,  G01N30/7233 ,  G01N30/74 ,  G01N33/15 ,  G01N33/6848

摘要： The present technology relates to a method of analyzing a sample including an analyte. The method includes injecting the sample including the analyte into a mobile phase. The mobile phase includes a metal chelator additive having a concentration between about 1 ppm to about 10 ppm. The method also includes separating the analyte using liquid chromatography and analyzing the analyte using a mass spectrometer, an ultra-violet detector, or a combination thereof.

公开(公告)号：US11906408B2

公开(公告)日：2024-02-20

申请号：US17394609

申请日：2021-08-05

申请人： Thermo Finnigan LLC

发明人： Daniel Lopez Ferrer ,  Aaron M. Robitaille ,  Michael O. Krawitzky ,  Greg A. Foster

IPC分类号： G01N1/40 ,  G01N33/68

CPC分类号： G01N1/4044 ,  G01N33/6848

摘要： A method for preparing a protein-containing sample for analysis by mass spectrometry includes introducing the sample into a reaction vessel. The reaction vessel contains a reagent mixture including pre-measured quantities of an immobilized proteolytic enzyme, a reducing agent and an alkylating agent. The contents of the reaction vessel are activated by heating or by sonication.

公开(公告)号：US20240027313A1

公开(公告)日：2024-01-25

申请号：US18254184

申请日：2021-11-24

申请人： PROVIGATE INC.

发明人： Mitsumi NISHI ,  Noriko MIYAUCHI ,  Yuuya MIYAZAWA

IPC分类号： G01N1/40

CPC分类号： G01N1/4005 ,  G01N1/4044 ,  G01N2001/4016

摘要： According to an embodiment of the present disclosure, a medium with which blood plasma is separated and stored, the medium including a porous medium for separation of blood plasma and a substance supported on the porous medium, the substance that suppresses glycation of a protein by glucose, is provided.

公开(公告)号：US20240011888A1

公开(公告)日：2024-01-11

申请号：US18251598

申请日：2021-10-26

申请人： Technische Universität München

发明人： Oliver HAYDEN ,  Katja PESCHKE ,  Maximilian REICHERT ,  Christian KLENK ,  Percy KNOLLE ,  Bastian HÖCHST

IPC分类号： G01N15/14 ,  B01L3/00 ,  G01N1/40

CPC分类号： G01N15/1463 ,  B01L3/502776 ,  G01N1/4044 ,  G01N15/1459 ,  G01N2001/4094 ,  G01N2015/1454 ,  B01L2400/0478 ,  G01N2001/383

摘要： Disclosed herein is a method of analyzing a tissue sample using a quantitative phase-contrast microscope as well as a corresponding microfluidic system and a corresponding device. The method comprises providing the tissue sample in a sample volume of a microfluidic system, wherein the tissue sample comprises a plurality of biological cells forming a continuous tissue material. At least a part of the tissue sample is dissolved into single cells and/or cell aggregates in a carrier fluid in the sample volume. A flow of the carrier fluid is generated from the sample volume to a measurement volume of the microfluidic system and a first phase shift image of the single cells and/or cell aggregates in the measurement volume is taken with the quantitative phase-contrast microscope.

公开(公告)号：US11821824B2

公开(公告)日：2023-11-21

申请号：US15707270

申请日：2017-09-18

申请人： Koligo Therapeutics, Inc.

发明人： Rolf Wolters ,  Anthony Yang ,  Josh Nelson ,  Stuart K. Williams

IPC分类号： C12M3/00 ,  B01L3/00 ,  A61M1/00 ,  G01N1/34 ,  C12N5/077 ,  G01N1/40 ,  C12N5/0775 ,  A61K35/35 ,  C12M1/34

CPC分类号： G01N1/34 ,  A61K35/35 ,  A61M1/79 ,  C12M41/00 ,  C12N5/0653 ,  C12N5/0667 ,  G01N1/4044 ,  A61M1/73 ,  A61M2202/0014 ,  A61M2202/0071 ,  A61M2202/0085 ,  A61M2202/08 ,  A61M2202/09 ,  C12N2509/10

摘要： Systems and methods herein are directed towards the separation of biologic material to obtain a target cell volume and/or cell concentration for harvesting. The target volume and/or concentration of cells may be obtained through a single cycle via three chambers, or by repeated cycles through one or more chambers to dilute the digestive enzymes used in the process and concentrate the harvestable cell volume to a predetermined target.

公开(公告)号：US11753636B2

公开(公告)日：2023-09-12

申请号：US16756851

申请日：2018-10-18

申请人： INFUSION TECH

发明人： Yong Shin ,  Choong Eun Jin

IPC分类号： C12N15/10 ,  G01N1/40

CPC分类号： C12N15/1006 ,  G01N1/4044

摘要： The present invention relates to a method of enriching a pathogen using an homobifunctional imidoester group, and the method of enriching a pathogen and extracting nucleic acids using homobifunctional imidoester compound (DMA, DMP, DMS) according to the present invention can quickly extract a small amount of pathogen contained in a sample without the use of special equipment and can be used as in situ diagnosis, and since it is possible to enrich a pathogen and extract nucleic acids simultaneously in one tube or a chip, it has the advantage of being more efficient than the conventional method, saving time and cost, and being easy to use.

公开(公告)号：US20230219087A1

公开(公告)日：2023-07-13

申请号：US17841470

申请日：2022-06-15

申请人： CytoVale Inc.

发明人： Henry Tat Kwong Tse ,  Ajay M. Shah ,  Lionel Guillou ,  Alexander Malkin ,  Anne E. Jensen ,  Nicholas Martinez ,  Carissa Colegrove ,  Laura Voyen

IPC分类号： B01L3/00 ,  G01N15/14 ,  G01N33/50 ,  G01N1/40 ,  G01N1/34 ,  G16B40/20

CPC分类号： B01L3/502761 ,  B01L3/502715 ,  G01N15/1434 ,  G01N33/5094 ,  G01N1/4077 ,  G01N1/34 ,  G01N1/4044 ,  G16B40/20 ,  G01N2001/4088 ,  G01N2015/0069

摘要： A system or method for detecting an immune system activation state in a patient can include a sample preparation system configured to isolate white blood cells from a sample of the patient, a cytometry module configured to determine biophysical properties of the white blood cells of the sample, and an analysis module configured to analyze the biophysical properties.

公开(公告)号：US20180217036A1

公开(公告)日：2018-08-02

申请号：US15747517

申请日：2016-08-16

申请人： IAS Inc.

发明人： Katsuhiko Kawabata ,  Tatsuya Ichinose ,  Hayashi Takuma

IPC分类号： G01N1/40 ,  G01N1/32 ,  G01N27/62 ,  H01L21/677 ,  H01L21/68 ,  H01L21/67

CPC分类号： G01N1/4044 ,  G01N1/28 ,  G01N1/32 ,  G01N27/62 ,  H01L21/67017 ,  H01L21/67034 ,  H01L21/67069 ,  H01L21/67253 ,  H01L21/6773 ,  H01L21/67742 ,  H01L21/68

摘要： A silicon substrate analyzing device with which impurities such as trace metals in a silicon substrate having a thick nitride film or oxide film formed on a silicon substrate surface can be analyzed with a high precision by ICP-MS. The silicon substrate analyzing device includes a load port, a substrate transportation robot, an aligner, a drying chamber, a gas-phase decomposition chamber, an analysis scan port having an analysis stage and a substrate analyzing nozzle, an analysis liquid collecting means, and an analysis means for performing inductively coupled plasma mass spectrometry. The silicon substrate having an oxide film or a nitride film formed on the silicon substrate is subjected to scanning the surface of the silicon substrate with a high-concentration recovered liquid with use of the substrate analyzing nozzle so that the high-concentration recovered liquid is recovered. The recovered high-concentration recovered liquid is discharged onto the surface of the silicon substrate and then heated and dried. The surface of the silicon substrate is scanned with the analysis liquid so that the impurities are recovered, and the analysis liquid is analyzed by ICP-MS.