公开(公告)号：US20110002526A1

公开(公告)日：2011-01-06

申请号：US12769057

申请日：2010-04-28

Applicant: Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

Inventor： Magalie Faivre ,  Patrice Caillat ,  Myriam-Laure Cubizolles ,  Christine Peponnet ,  Claude Vauchier

IPC: G06K9/78

CPC classification number: G01N33/86

Abstract: A method of counting thrombocytes contained in a sample of blood, the method having the steps: mixing said sample with: fluorescent markers suitable for bonding specifically with the thrombocytes; and an agent for inhibiting activation of said thrombocytes; introducing the sample (E) into a fluidic chamber (CF) having at least one transparent face; acquiring at least one digital image (IN) of said sample by fluorescence microscopy under stationary illumination; and counting the thrombocytes (T) present in said or each image by image processing computer means (O). Apparatus for implementing the method.

Abstract translation: 一种对血液样品中含有的血小板计数的方法，所述方法具有以下步骤：将所述样品与适合于与凝血细胞特异性结合的荧光标记物混合; 和用于抑制所述血小板激活的药剂; 将样品（E）引入到具有至少一个透明面的流体室（CF）中; 在静止照明下通过荧光显微镜获取所述样品的至少一个数字图像（IN）; 以及通过图像处理计算机装置（O）计数存在于所述图像或每个图像中的血小板（T）。 用于实施该方法的装置。

公开(公告)号：US06977145B2

公开(公告)日：2005-12-20

申请号：US09772280

申请日：2001-01-29

Applicant: Yves Fouillet ,  Claude Vauchier ,  Jean-Frederic Clerc ,  Christine Peponnet ,  Patricia Claustre ,  Raymond Charles ,  Nicolas Sarrut

Inventor： Yves Fouillet ,  Claude Vauchier ,  Jean-Frederic Clerc ,  Christine Peponnet ,  Patricia Claustre ,  Raymond Charles ,  Nicolas Sarrut

IPC: G01N33/50 ,  B01J19/00 ,  B01L3/00 ,  B01L7/00 ,  B01L7/02 ,  B81B1/00 ,  B81C1/00 ,  C12M1/00 ,  C12M1/22 ,  C12M1/34 ,  C12M1/38 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N1/28 ,  G01N35/02 ,  G01N35/08 ,  G01N37/00 ,  C12M1/36 ,  C12M3/00

CPC classification number: G01N35/08 ,  B01L3/5025 ,  B01L3/5027 ,  B01L3/502707 ,  B01L3/502746 ,  B01L3/502784 ,  B01L7/02 ,  B01L7/52 ,  B01L7/525 ,  B01L7/5255 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/0845 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1838 ,  B01L2300/1883 ,  B01L2400/0415 ,  B01L2400/0487 ,  F28F2260/02

Abstract: Devices and methods for carrying out a chemical or biochemical protocol are disclosed. In one embodiment, the chemical or biochemical protocol is performed by cycling at least one thermal transfer member between at least two temperatures while liquid samples on which the chemical or biochemical protocol is to be performed are continuously moving through at least one temperature regulated zone upon which the at least one thermal transfer member acts. In some embodiments, the device comprises a sample transport member that comprises liquid samples in sample receiving regions. The sample transport member moves the samples continuously through a temperature regulated zone which cycles between at least two temperatures while the liquid samples are moving through a temperature regulated zone on which at least one thermal transfer member acts. In some embodiments, the sample receiving regions comprise wells, hydrophillic films or hydrophillic filaments.

公开(公告)号：US06274351B1

公开(公告)日：2001-08-14

申请号：US08836031

申请日：1997-07-11

Applicant: Christine Peponnet

Inventor： Christine Peponnet

IPC: C12P1934

CPC classification number: C12Q1/6837 ,  C12Q1/6846 ,  C12Q2565/537 ,  C12Q2525/197

Abstract: A support for solid phase amplification or sequencing of nucleic acids has a functionalized solid support, a linker arm having functional groups covalently bound to the solid support through at least one binding site of the functional groups, and an oligonucleotide primer bound at its 5′ end to the linker, the primer, thereby, being immobilized on the solid support.

Abstract translation: 对核酸的固相扩增或测序的支持物具有官能化的固体支持物，具有通过官能团的至少一个结合位点与固体支持物共价结合的官能团的接头臂和在其5'端结合的寡核苷酸引物 连接体，引物，从而固定在固体支持物上。