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    Method for preferentially removing protein over nucleic acids using physical as well as chemical means of removal of the protein
    13.
    发明授权
    Method for preferentially removing protein over nucleic acids using physical as well as chemical means of removal of the protein 有权
    使用物理以及去除蛋白质的化学方法优先去除蛋白质的方法

    公开(公告)号：US07435811B2

    公开(公告)日：2008-10-14

    申请号：US11396761

    申请日：2006-04-03

    Applicant: Myo-yong Lee ,  Ki-woong Han

    Inventor： Myo-yong Lee ,  Ki-woong Han

    IPC: C07H21/00

    CPC classification number: C07K1/36 ,  C07K1/20 ,  C12Q1/6806 ,  C12Q2527/125

    Abstract: Provided is a method of removing protein while not removing nucleic acids from a biological sample containing protein, the method including: adding a compound of formula I below and a protein nucleating agent to the biological sample containing protein: where at least two of R1, R2, and R3 substituents are substituted or unsubstituted C1-C6 alkyl groups and the other substituent is a hydrogen atom or a substituted or unsubstituted C1-C6 alkyl group, a is an integer of 1 to 6, and b is 0 or 1, wherein b is 0 when a is not 1; treating the resultant mixture with a hydrophobic surface material in order to obtain a protein-free mixture; and separating the protein-free mixture from the hydrophobic surface material to which the protein is bound. By using the method, the protein can be selectively, effectively removed from the biological sample containing the protein while a nucleic acid is maintained in the sample.

    Abstract translation: 提供一种除去蛋白质而不从含有生物样品的蛋白质中除去核酸的方法，该方法包括：将下面的式I化合物和蛋白质成核剂加入到含有蛋白质的生物样品中：其中至少两个R R 1，R 2，R 3和R 3取代基是取代或未取代的C 1 -C 6烷基，而另一个取代基是氢原子或取代或未取代的 C1-C6烷基，a为1〜6的整数，b为0或1，a为1时，b为0; 用疏水性表面材料处理所得混合物以获得无蛋白质的混合物; 并将蛋白质与蛋白质结合的疏水表面材料分离出来。 通过使用该方法，可以在将样品中保持核酸的同时，从含有蛋白质的生物样品中有效地除去蛋白质。

    METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER
    14.
    发明申请
    METHOD AND APPARATUS FOR THE RAPID DISRUPTION OF CELLS OR VIRUSES USING MICRO BEADS AND LASER 审中-公开
    使用微珠和激光快速破坏细胞或病毒的方法和装置

    公开(公告)号：US20080199930A1

    公开(公告)日：2008-08-21

    申请号：US12031947

    申请日：2008-02-15

    Applicant: Jeong-gun LEE ,  Young-nam KWON ,  Young-a KIM ,  Myo-yong LEE ,  Shin-i YOO ,  Yeon-ja CHO ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Seung-yeon YANG

    Inventor： Jeong-gun LEE ,  Young-nam KWON ,  Young-a KIM ,  Myo-yong LEE ,  Shin-i YOO ,  Yeon-ja CHO ,  Kwang-ho CHEONG ,  Chang-eun YOO ,  Seung-yeon YANG

    IPC: C12N13/00 ,  C12M1/00

    CPC classification number: C12M47/06 ,  B01F11/0266 ,  B01F13/0059 ,  B01L3/502761 ,  B01L2300/0816 ,  C12N1/066 ,  C12N13/00

    Abstract: A method and apparatus for rapid disruption of cells or viruses using beads and a laser are provided. According to the method and apparatus for rapid disruption of cells or viruses using beads and a laser, cell lysis within 40 seconds is possible, the apparatus can be miniaturized using a laser diode, a DNA purification step can be directly performed after a disruption of cells or viruses, and a solution containing DNA can be transferred to a subsequent step after cell debris and beads to which inhibitors of a subsequent reaction are attached are removed with an electromagnet. In addition, by means of the cell lysis chip, an evaporation problem is solved, vibrations can be efficiently transferred to cells through magnetic beads, a microfluidics problem on a rough surface is solved by hydrophobically treating the inner surface of the chip, and the cell lysis chip can be applied to LOC.

    Abstract translation: 提供了使用珠和激光来快速破坏细胞或病毒的方法和装置。 根据使用珠粒和激光快速破坏细胞或病毒的方法和装置，可以在40秒内进行细胞裂解，可以使用激光二极管使装置小型化，DNA纯化步骤可以在细胞破碎后直接进行 或病毒，并且含有DNA的溶液可以转移到随后的步骤中，在用电磁体除去后续反应的抑制剂附着的细胞碎片和珠粒之后。 另外，通过细胞裂解芯片，解决了蒸发问题，可以通过磁珠有效地将振动传递到细胞，粗糙表面上的微流体问题通过疏水处理芯片的内表面和电池来解决 裂解芯片可应用于LOC。

    Microarray reaction device and method of using the same
    15.
    发明授权
    Microarray reaction device and method of using the same 失效
    微阵列反应装置及其使用方法

    公开(公告)号：US08647591B2

    公开(公告)日：2014-02-11

    申请号：US13007750

    申请日：2011-01-17

    Applicant: Won-seok Chung ,  Myo-yong Lee ,  Kak Namkoong ,  Woochang Lee

    Inventor： Won-seok Chung ,  Myo-yong Lee ,  Kak Namkoong ,  Woochang Lee

    IPC: B01L3/00 ,  B01L99/00 ,  F16K3/00 ,  C40B40/00 ,  C40B60/00 ,  F16K31/00

    CPC classification number: C40B30/00 ,  C40B40/06 ,  C40B40/10 ,  C40B60/12

    Abstract: A microarray reaction device includes a fluid container, a reaction chamber, a first channel connected with the fluid container, a second channel connected with the reaction chamber, and a valve. The valve includes a first and second support unit, respectively including a first and second penetration opening unit, extended through a first and second surface thereof. The first and second penetration opening unit is connected to a second end of the first and second channel, respectively. The second support unit includes a third penetration opening unit extended through a second surface thereof. The first and second surfaces contact each other, such that the first support unit and the second support unit are slidably disposed with each other. The microarray reaction device further includes a storing chamber connected with the third penetration opening unit, and a pump connected to the storing chamber and providing pressure to the storing chamber.

    Abstract translation: 微阵列反应装置包括流体容器，反应室，与流体容器连接的第一通道，与反应室连接的第二通道和阀。 阀包括第一和第二支撑单元，分别包括延伸穿过其第一和第二表面的第一和第二穿透开口单元。 第一和第二穿透开口单元分别连接到第一和第二通道的第二端。 第二支撑单元包括延伸穿过其第二表面的第三穿透开口单元。 第一和第二表面彼此接触，使得第一支撑单元和第二支撑单元彼此可滑动地布置。 微阵列反应装置还包括与第三穿透开口单元连接的储存室，以及连接到储存室并向储存室提供压力的泵。

    DEVICE FOR HYBRIDIZATION CHAMBERS PREVENTING AIR BUBBLES AND HYBRIDIZATION DEVICE COMPRISING THE SAME DEVICE
    17.
    发明申请
    DEVICE FOR HYBRIDIZATION CHAMBERS PREVENTING AIR BUBBLES AND HYBRIDIZATION DEVICE COMPRISING THE SAME DEVICE 审中-公开
    用于防止空气泡沫的混合室的装置和包含相同装置的混合装置

    公开(公告)号：US20090215653A1

    公开(公告)日：2009-08-27

    申请号：US11850917

    申请日：2007-09-06

    Applicant: Myo-yong LEE

    Inventor： Myo-yong LEE

    IPC: C40B60/00

    CPC classification number: B01L3/508 ,  B01L9/527 ,  B01L2200/0684 ,  G01N2035/00306

    Abstract: A device for a hybridization chamber includes a support including an engagement receiving member which receives a microarray, the engagement receiving member engages with the microarray, a hybridization chamber frame which forms the hybridization chamber when in contact with the microarray, a sealing member disposed on the hybridization frame, the sealing member defines a region of the hybridization chamber and a cover coupled with the support and the hybridization chamber frame, wherein one end of the cover is coupled with the support using a hinge, and an opposite end of the cover includes a compression coupling means.

    Abstract translation: 用于杂交室的装置包括支撑件，其包括接收微阵列的接合接收构件，接合接收构件与微阵列接合，当与微阵列接触时形成杂交室的杂交室框架，设置在微阵列上的密封构件 杂交框架，密封构件限定杂交室的区域和与支撑件和杂交室框架联接的盖，其中盖的一端使用铰链与支撑件联接，并且盖的相对端包括 压缩联接装置。

    METHOD OF PURIFYING RNA USING KOSMOTROPIC SALT
    20.
    发明申请
    METHOD OF PURIFYING RNA USING KOSMOTROPIC SALT 有权
    使用KOSMOTROPIC盐净化RNA的方法

    公开(公告)号：US20090048437A1

    公开(公告)日：2009-02-19

    申请号：US12110391

    申请日：2008-04-28

    Applicant: Myo-yong LEE ,  Nam Huh ,  Joon-ho Kim

    Inventor： Myo-yong LEE ,  Nam Huh ,  Joon-ho Kim

    IPC: C07H21/02

    CPC classification number: C07H21/02 ,  C12N15/1003 ,  C12N15/1006

    Abstract: The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.

    Abstract translation: 本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。 根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。 本发明在纯化200个或更少核苷酸的RNA中特别有效。

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