Integrated Droplet Actuator for Gel Electrophoresis and Molecular Analysis
    12.
    发明申请
    Integrated Droplet Actuator for Gel Electrophoresis and Molecular Analysis 有权
    用于凝胶电泳和分子分析的集成滴液致动器

    公开(公告)号:US20150323496A1

    公开(公告)日:2015-11-12

    申请号:US14795304

    申请日:2015-07-09

    IPC分类号: G01N27/447 B01L3/00

    摘要: The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator.

    摘要翻译: 本发明涉及液滴致动器装置以及用于将凝胶电泳分析与分析前样品或分析后处理以及其它分子分析方法相集成的方法。 使用数字微流体技术,液滴致动器装置和本发明的方法提供了在单个集成装置上执行凝胶电泳和液体处理操作的能力。 集成的液体处理操作可以用于制备并将样品递送到电泳凝胶,捕获并随后处理电泳凝胶的产物,或在通过凝胶电泳分析的相同样品材料上进行附加测定。 在一个实施方案中,在与电泳凝胶结合的液滴致动器上进行一个或多个分子测定,例如通过实时PCR的核酸(例如DNA)定量和一个或多个样品处理操作,例如样品稀释。 在一个实施方案中,电泳凝胶可以集成在液滴致动器的顶部基底上。

    Droplet-Based Nucleic Acid Amplification Method and Apparatus
    14.
    发明申请
    Droplet-Based Nucleic Acid Amplification Method and Apparatus 审中-公开
    基于液滴的核酸扩增方法和装置

    公开(公告)号:US20160016172A1

    公开(公告)日:2016-01-21

    申请号:US14837133

    申请日:2015-08-27

    IPC分类号: B01L7/00 G01N27/447

    摘要: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.

    摘要翻译: 本发明的实施例的方面涉及一种模拟器,其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器,以及包括电子耦合到能够执行指令的处理器并由其控制的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。

    Droplet-based nucleic acid amplification method and apparatus
    15.
    发明授权
    Droplet-based nucleic acid amplification method and apparatus 有权
    基于液滴的核酸扩增方法和装置

    公开(公告)号:US09139865B2

    公开(公告)日:2015-09-22

    申请号:US13680979

    申请日:2012-11-19

    IPC分类号: G05D5/06 C12Q1/68 G01N27/447

    摘要: Aspects of embodiments of the invention relate to a simulator including a visual display capable of outputting to a user a display one or more effects of a command series selected and a system including a droplet microactuator electronically coupled to and controlled by a processor capable of executing instructions, the droplet microactuator including a substrate comprising electrodes for conducting droplet operations. Further aspects of embodiments of the invention relate to a droplet operations troubleshooting apparatus. Other aspects of embodiments of the invention relate to a computer implemented method of displaying simulated microactuator droplets.

    摘要翻译: 本发明的实施例的方面涉及一种模拟器,其包括能够向用户输出显示所选择的命令序列的一个或多个效果的视觉显示器,以及包括电子耦合到能够执行指令的处理器并由控制器执行指令的液滴微致动器的系统 液滴微致动器包括包括用于进行液滴操作的电极的基板。 本发明的实施例的其它方面涉及一种液滴操作故障排除装置。 本发明的实施例的其他方面涉及一种显示模拟微致动器液滴的计算机实现的方法。