公开(公告)号：US06697508B2

公开(公告)日：2004-02-24

申请号：US10143064

申请日：2002-05-10

Applicant: Alan C. Nelson

Inventor： Alan C. Nelson

IPC: G06K900

CPC classification number: G06T11/006 ,  G06T2211/424

Abstract: Three-dimensional (3D) reconstruction of a cell includes adjusting a current set of projection images according to a priori knowledge to produce adjusted projection images, for example, based on probability masks and/or Bayesian analysis of multiple similar objects in the same sample. A reconstruction algorithm processes the adjusted projection images to generate a 3D image. The 3D image is further adjusted according to the a priori knowledge to generate an adjusted 3D image. Criteria for process completion are applied to determine whether the adjusted 3D image is adequate. Otherwise, a set of pseudo projections are computationally created at the same projection angles as the current set of projection images and then compared to the current set of projection images to produce a set of new projections, wherein the new projections are input again to the reconstruction algorithm and the steps of the method are repeated until the adequacy criteria are met.

Abstract translation: 单元的三维（3D）重构包括根据先验知识调整当前投影图像集合，以产生调整的投影图像，例如，基于同一样本中多个相似对象的概率掩模和/或贝叶斯分析。 重建算法处理调整的投影图像以产生3D图像。 根据先验知识进一步调整3D图像以产生经调整的3D图像。 应用流程完成的标准来确定调整后的3D图像是否足够。 否则，以与当前投影图像集相同的投影角度计算地创建一组伪投影，然后与当前的投影图像组进行比较以产生一组新的投影，其中新的投影再次输入到重建 重复算法和方法的步骤，直到满足适当性标准。

公开(公告)号：US5627908A

公开(公告)日：1997-05-06

申请号：US309063

申请日：1994-09-20

Applicant: Shih-Jong J. Lee ,  Alan C. Nelson ,  Larry A. Nelson ,  Carl E. Youngmann ,  Keith L. Frost

Inventor： Shih-Jong J. Lee ,  Alan C. Nelson ,  Larry A. Nelson ,  Carl E. Youngmann ,  Keith L. Frost

IPC: G01N15/14 ,  G06K9/00 ,  G06K9/38

CPC classification number: G01N15/1475

Abstract: A cytological system dynamic normalization of a normal threshold. An analysis score from a slide is compared against a threshold to determine whether or not the slide is normal or requires microscopy review. The normal threshold is dynamically adjusted using a three step process. The process is implemented on an automatic cytology system. The first step is initial calibration of the system to determine an initial threshold. The second step is a running adjustment of the normal threshold in response to the presentation of new slides to the automatic cytology system. The third step is the batch certification of every slide. The threshold may be adjusted for an analysis score, a quality control score, or a screening score.

Abstract translation: 细胞学系统动态归一化正常阈值。 将幻灯片的分析得分与阈值进行比较，以确定幻灯片是否正常或需要显微镜检查。 使用三步过程动态调整正常阈值。 该过程在自动细胞学系统上实现。 第一步是系统的初始校准，以确定初始阈值。 第二步是响应于向自动细胞学系统呈现新幻灯片而正常阈值的运行调整。 第三步是每张幻灯片的批量认证。 可以针对分析得分，质量控制评分或筛选分数调整阈值。

公开(公告)号：US07494809B2

公开(公告)日：2009-02-24

申请号：US11353353

申请日：2006-02-14

Applicant: Alan C. Nelson ,  Florence W. Patten

Inventor： Alan C. Nelson ,  Florence W. Patten

IPC: C12N5/06 ,  C12M3/00

CPC classification number: C12M47/04 ,  C12M47/02

Abstract: A method for automated cell enrichment preparation where biological cells are injected into a first subchamber and flowed through the first subchamber, through a large pore filter and into a second subchamber so that the large pore filter retains particles large particles in the first subchamber, while the biological cells pass through the first filter into the second subchamber and are retained by a small pore filter. After clearing, the biological cells are stained. A solvent exchange leaves the biological cells in solvent and they are released into a concentration module where a portion of the solvent is removed to form an enriched concentrated cell suspension.

Abstract translation: 一种用于自动细胞富集制备的方法，其中将生物细胞注入第一子室并流过第一子室，通过大孔过滤器并进入第二子室，使得大孔过滤器在第一子室中保留颗粒大颗粒，而 生物细胞通过第一过滤器进入第二子室并由小孔过滤器保留。 清除后，生物细胞染色。 溶剂交换将溶剂中的生物细胞离开，并将其释放到浓缩模块中，其中去除一部分溶剂以形成富集的浓缩细胞悬液。

公开(公告)号：US07260253B2

公开(公告)日：2007-08-21

申请号：US10876328

申请日：2004-06-24

Applicant: John Richard Rahn ,  Alan C. Nelson

Inventor： John Richard Rahn ,  Alan C. Nelson

IPC: G06K9/00 ,  G21K1/12

CPC classification number: G02B21/26 ,  G01N15/1468 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/05 ,  G01N21/4795 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2021/0346 ,  G01N2021/1787 ,  G01N2201/0813 ,  G01N2223/612 ,  G02B21/365 ,  G06T11/005 ,  G06T11/006 ,  G06T2211/412 ,  G06T2211/421

Abstract: Motion correction for optical tomographic imaging in three dimensions. An object of interest is illuminated to produce an image. A lateral offset correction value is determined for the image. An axial offset correction value is determined for the image. The lateral offset correction value and the axial offset correction value are applied to the image to produce a corrected file image.

Abstract translation: 光学断层摄影三维运动校正。 感兴趣的对象被照亮以产生图像。 确定图像的横向偏移校正值。 为图像确定轴向偏移校正值。 将横向偏移校正值和轴向偏移校正值应用于图像以产生校正的文件图像。

公开(公告)号：US06944322B2

公开(公告)日：2005-09-13

申请号：US10308309

申请日：2002-12-03

Applicant: Roger H. Johnson ,  Alan C. Nelson

Inventor： Roger H. Johnson ,  Alan C. Nelson

IPC: G01N15/14 ,  G01N21/03 ,  G01N21/05 ,  G01N21/17 ,  G01N21/59 ,  G01N21/64 ,  G01N21/78 ,  G01N23/04 ,  G01N33/543 ,  G01N33/554 ,  G01N33/60 ,  G06T1/00 ,  G06T3/00 ,  G06T11/00 ,  G06K9/00

CPC classification number: G01N21/4795 ,  G01N15/14 ,  G01N15/147 ,  G01N21/0303 ,  G01N21/05 ,  G01N21/6428 ,  G01N21/6456 ,  G01N23/046 ,  G01N2021/0307 ,  G01N2021/0392 ,  G01N2021/052 ,  G01N2021/1772 ,  G01N2021/1787 ,  G01N2021/5919 ,  G01N2021/5923 ,  G01N2021/5957 ,  G01N2201/0696 ,  G01N2223/419 ,  G06T11/006

Abstract: A parallel-beam optical tomography system for imaging an object of interest includes a parallel ray beam radiation source that illuminates the object of interest with a plurality of parallel radiation beams. After passing through the object of interest the pattern of transmitted or emitted radiation intensities is magnified by a post specimen optical element or elements. An object containing tube is located within an outer tube, wherein the object of interest is held within or flows through the object containing tube. A motor may be coupled to rotate and/or translate the object containing tube to present differing views of the object of interest. One or more detector arrays are located to receive the emerging radiation from the post specimen magnifying optic. Two- or three-dimensional images may be reconstructed from the magnified parallel projection data.

Abstract translation: 用于对感兴趣对象进行成像的平行光束光学层析成像系统包括用多个平行辐射束照亮感兴趣对象的平行射线束辐射源。 在通过感兴趣的对象之后，透射或发射的辐射强度的图案被后检体光学元件放大。 物体容纳管位于外管内，其中感兴趣的物体保持在物体容纳管内或流过物体容纳管。 马达可以联接以旋转和/或平移物体容纳管，以呈现感兴趣物体的不同视图。 定位一个或多个检测器阵列以接收来自后检体放大镜的新出射的辐射。 可以从放大的平行投影数据重构二维或三维图像。

公开(公告)号：US06636623B2

公开(公告)日：2003-10-21

申请号：US10145982

申请日：2002-05-14

Applicant: Alan C. Nelson ,  Robert W. Webster ,  Chee-Wui Chu

Inventor： Alan C. Nelson ,  Robert W. Webster ,  Chee-Wui Chu

IPC: G06K900

CPC classification number: G01N33/5091 ,  G01N15/1475 ,  G01N23/046 ,  G01N33/574 ,  G01N2015/1006 ,  G01N2223/419 ,  G06K9/00127 ,  G06T7/0012 ,  G06T2207/10152 ,  G06T2207/30024

Abstract: A system and method for rapidly detecting cells associated with malignancy and disease using molecular marker compartmentalization includes an optical tomography (OT) or a flow optical tomography (FOT) instrument capable of producing various optical projection images (or shadowgrams) containing accurate density information from a cell or cells labeled with tagged molecular probes or stains, a computer and software to analyze and reconstruct the projection images into a multi-dimensional data set, and automated feature collection and object classifiers. The system and method are particularly useful in the early detection of cancers such as lung cancer using cells from sputum or cheek scrapings and cervical/ovarian cancer using a cervical scraping, and the system can be used to detect rare cells in specimens including blood.

公开(公告)号：US4038943A

公开(公告)日：1977-08-02

申请号：US588573

申请日：1975-06-20

Applicant: Alan C. Nelson ,  Lothar S. Jeromin

Inventor： Alan C. Nelson ,  Lothar S. Jeromin

IPC: G03G15/08

CPC classification number: G03G15/08

Abstract: Method and apparatus for reducing X-ray dosage required for xeroradiographic examinations without reducing the relative information capacity of the images produced during the examination. In particular, a charged xerographic plate is positioned adjacent the object to be examined and penetrating X-ray radiation is projected through the object onto the plate surface, forming a latent electrostatic image on the surface of the plate. The penetrating radiation utilized is of a substantially lower dosage than normally utilized. The image is then partially developed with developing powder and the partially developed image is then charged and exposed to substantially uniform radiation. The exposed charged image is finally developed by applying additional developing powder thereto resulting in an enhanced image or signal.

Abstract translation: 降低Xeroriography检查所需X射线剂量的方法和装置，而不会降低检查期间产生的图像的相对信息容量。 特别地，带电的静电复印板被定位成与被检查物体相邻，并且穿透X射线辐射通过物体投射到板表面上，在该板的表面上形成静电潜像。 所使用的穿透辐射具有比正常使用的显着更低的剂量。 然后将图像用显影粉末部分显影，然后将部分显影的图像充电并暴露于基本均匀的辐射。 曝光的带电图像最终通过施加额外的显影粉末而形成，从而产生增强的图像或信号。

公开(公告)号：US08368035B2

公开(公告)日：2013-02-05

申请号：US13402149

申请日：2012-02-22

Applicant: Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

Inventor： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

IPC: G01N21/64

CPC classification number: G01N21/4795 ,  G01N15/1436 ,  G01N2015/1445 ,  G01N2021/1787 ,  G06T11/006

Abstract: A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

Abstract translation: 用于光学断层摄影系统中的细胞的3D成像的方法包括相对于显微镜物镜移动生物体以呈现不同的视角。 用具有限制在150nm和390nm之间的波长的光谱带宽的辐射照射该生物物体。 利用摄像机从多个不同的视角检测透过生物物体和显微镜物镜的辐射。 形成来自感测辐射的生物物体的多个伪投影，并重建多个伪投影以形成该细胞的3D图像。

公开(公告)号：US06991738B1

公开(公告)日：2006-01-31

申请号：US10964513

申请日：2004-10-13

Applicant: Mark E. Fauver ,  Alan C. Nelson

Inventor： Mark E. Fauver ,  Alan C. Nelson

IPC: B01D21/26 ,  B01D37/02

CPC classification number: B04B5/0442 ,  B04B1/02 ,  B04B15/08 ,  B04B2005/0478 ,  B04B2005/0485 ,  G01N1/2813 ,  G01N2001/2846

Abstract: A process includes coating a cylinder having an inner wall and a cylinder axis with a gel coating on the inner wall. Then a specimen mixture including solvent is made to flow through the cylinder while the cylinder is being continuously rotated. The specimen mixture is initially directed to flow along the cylinder axis and such that specimen particles from the specimen mixture are accelerated off the cylinder axis toward the inner wall, so as to form a film of specimen particles embedded into the gel coating.

Abstract translation: 一种方法包括在内壁上涂覆具有内壁和圆筒轴线的圆柱体，其中凝胶涂层涂覆在内壁上。 然后使包含溶剂的样品混合物在圆筒连续旋转的同时流过圆筒。 样品混合物最初被引导为沿着圆柱体轴线流动，使得来自样品混合物的样品颗粒从圆筒轴线朝向内壁加速，以形成嵌入凝胶涂层中的样品颗粒的膜。

公开(公告)号：US06770893B2

公开(公告)日：2004-08-03

申请号：US10147154

申请日：2002-05-13

Applicant: Alan C. Nelson

Inventor： Alan C. Nelson

IPC: G01N2184

CPC classification number: G21K5/10 ,  G01N21/4795 ,  G01N21/6456 ,  G01N33/48728 ,  G01N2021/6439

Abstract: An optical tomographic system wherein the localization of secondary emitters within an object of interest is determined using the temporal signatures of secondary emission arising from the object being illuminated by an external primary source beam that is non-parallel, such as a cone beam, while the object is moving relative to said beam in a controlled manner. A unique set of secondary emitter spots is localized within the object and, when combined with a computed 3D reconstruction of the object from its primary cone beam projections, creates an image of the secondary emitters in the object so as to enable quantitative three-dimensional imaging of fluorescent labeled molecular probes in a biological cell, for example.

Abstract translation: 一种光学断层摄影系统，其中使用由不平行的外部主源光束（例如锥形束）照射的物体产生的二次发射的时间特征来确定二次发射器在感兴趣对象内的定位，而 物体以受控的方式相对于所述光束移动。 一个独特的二次发射极点集合在物体内，并且当与其主要锥形束投影的物体的计算的3D重建组合时，在对象中创建二次发射器的图像，以便能够进行定量三维成像 荧光标记的分子探针在生物细胞中。