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公开(公告)号：US11365381B2
公开(公告)日：2022-06-21
申请号：US16920365
申请日：2020-07-02
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, Jr. , Kristin G. Beaumont , Aathavan Karunakaran , Natalie C. Marks , Jason M. McEwen , Mark P. White , J. Tanner Nevill , Gang F. Wang , Andrew W. McFarland , Daniele Malleo , Keith J. Breinlinger , Xiao Guan , Kevin T. Chapman
Abstract: Systems, methods and kits are described for culturing one or more biological cells in a microfluidic device, including provision of nutrients and gaseous components configured to enhance cell growth, viability, portability, or any combination thereof. In some embodiments, culturing a single cell may produce a clonal population in the microfluidic device.
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公开(公告)号：US20220143612A1
公开(公告)日：2022-05-12
申请号：US17085185
申请日：2020-10-30
Applicant: Berkeley Lights, Inc.
Inventor： Keith J. Breinlinger , Eric D. Hobbs , Daniele Malleo , J. Tanner Nevill , Mark P. White
Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.
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公开(公告)号：US10252907B2
公开(公告)日：2019-04-09
申请号：US15439506
申请日：2017-02-22
Applicant: Berkeley Lights, Inc.
Inventor： Keith J. Breinlinger , Daniele Malleo , Gaetan L. Mathieu , J. Tanner Nevill , Alexander H. Slocum , Mark P. White
IPC: B81B7/02 , B03C5/00 , B03C5/02 , G01N27/447 , G01N33/50 , B03C7/02 , G01N33/543 , B01L3/00 , C12M3/06 , C12M1/00 , C12M1/34 , G01N35/10
Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.
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公开(公告)号：US20160199837A1
公开(公告)日：2016-07-14
申请号：US14965721
申请日：2015-12-10
Applicant: Berkeley Lights, Inc.
Inventor： Keith J. Breinlinger , Eric D. Hobbs , Daniele Malleo , J. Tanner Nevill , Mark P. White
CPC classification number: B01L3/502761 , B01L3/502715 , B01L3/50273 , B01L2200/0668 , B01L2300/0816 , B01L2300/0819 , B01L2300/0848 , B01L2300/0877 , B01L2400/0424 , B01L2400/0427 , B01L2400/0454 , B01L2400/0457 , B01L2400/086 , G01N1/4077 , G01N2001/4083
Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.
Abstract translation: 提供了一种微流体装置，其具有一个或多个隔离笔，其被配置为通过相对于诸如重力的全局有效力改变微流体装置的取向来隔离一个或多个目标微物体。 还提供了使用重力在微流体装置中选择性地引导微物体运动的方法。 微物体可以是生物微物体，例如细胞，或无生命的微物体，例如珠粒。
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15.发明申请Capturing Specific Nucleic Acid Materials From Individual Biological Cells In A Micro-Fluidic Device 审中-公开从微流体装置中的各种生物细胞捕获特定的核酸材料公开(公告)号：US20150166326A1
公开(公告)日：2015-06-18
申请号：US14133361
申请日：2013-12-18
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Eric D. Hobbs , Steven W. Short , Mark P. White
CPC classification number: B03C5/005 , B01L3/502761 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2400/0424 , B01L2400/0454 , B03C5/026 , B03C2201/26
Abstract: Individual biological cells can be selected in a micro-fluidic device and moved into isolation pens in the device. The cells can then be lysed in the pens, releasing nucleic acid material, which can be captured by one or more capture objects in the pens. The capture objects with the captured nucleic acid material can then be removed from the pens. The capture objects can include unique identifiers, allowing each capture object to be correlated to the individual cell from which the nucleic acid material captured by the object originated.
Abstract translation: 可以在微流体装置中选择各种生物细胞，并将其移动到装置中的隔离笔中。 然后，细胞可以在笔中裂解，从而释放核酸物质，其可被笔中的一个或多个捕获物捕获。 然后可以从笔中取出捕获的核酸材料的捕获物体。 捕获对象可以包括唯一的标识符，允许每个捕获对象与由对象捕获的核酸材料源自的单个细胞相关联。
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Patent Agency Ranking
16.发明授权公开(公告)号：US11971409B2
公开(公告)日：2024-04-30
申请号：US16892642
申请日：2020-06-04
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Mark P. White , Xiaohua Wang , Minha Park , Guido K. Stadler , Randall D. Lowe, Jr. , Xiao Guan Radstrom , Jason M. McEwen , Gang F. Wang , George L. Fox , Peggy A. Radel
IPC: B01L3/00 , C07K16/00 , C07K16/28 , C07K16/30 , C12Q1/6886 , G01N33/50 , G01N33/543 , G01N33/569 , G01N33/574
CPC classification number: G01N33/574 , B01L3/502761 , C07K16/00 , C07K16/2887 , C07K16/30 , C12Q1/6886 , G01N33/5047 , G01N33/505 , G01N33/5052 , G01N33/54366 , G01N33/56972 , B01L2200/0647 , G01N2800/7028
Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.
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公开(公告)号：US11801508B2
公开(公告)日：2023-10-31
申请号：US17162467
申请日：2021-01-29
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, Jr. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
IPC: B01L3/00 , C12M3/06 , B81C1/00 , G01N33/543
CPC classification number: B01L3/502707 , B01L3/502715 , B01L3/502761 , B81C1/00206 , C12M23/16 , G01N33/54393 , B01L2300/0636 , B01L2300/088 , B01L2300/0816 , B01L2300/0864 , B81B2201/058
Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
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公开(公告)号：US20210291171A1
公开(公告)日：2021-09-23
申请号：US17162467
申请日：2021-01-29
Applicant: Berkeley Lights, Inc.
Inventor： Randall D. Lowe, JR. , Alexander J. Mastroianni , Mark P. White , Gregory G. Lavieu , Kristin G. Beaumont
IPC: B01L3/00 , C12M3/06 , B81C1/00 , G01N33/543
Abstract: In biosciences and related fields, it can be useful to modify surfaces of apparatuses, devices, and materials that contact biomaterials such as biomolecules and biological micro-objects. Described herein are surface modifying and surface functionalizing reagents, preparation thereof, and methods for modifying surfaces to provide improved or altered performance with biomaterials.
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公开(公告)号：US20210069698A1
公开(公告)日：2021-03-11
申请号：US16928225
申请日：2020-07-14
Applicant: Berkeley Lights, Inc.
Inventor： Xiao Guan , Mark P. White , Jason M. McEwen , Gang F. Wang , Kevin T. Chapman , Xiaohua Wang , Christine E. Sun
Abstract: Functional assays using reporter cell assays are described which probe the activity of at least one cell of interest. The ability to probe at least one cell is provided by using the microfluidic methods, devices and kits described herein. Assays combining both reporter cell signaling as well as binding assay signaling for at least one cell is also described herein.
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公开(公告)号：US10821439B2
公开(公告)日：2020-11-03
申请号：US15659855
申请日：2017-07-26
Applicant: Berkeley Lights, Inc.
Inventor： Keith J. Breinlinger , Eric D. Hobbs , Daniele Malleo , J. Tanner Nevill , Mark P. White
Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.
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