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20 条记录 (耗时 0.026 秒)

    Calmodulin-based cell separation technique
    12.
    发明授权
    Calmodulin-based cell separation technique 失效
    基于钙调蛋白的细胞分离技术

    公开(公告)号：US5985658A

    公开(公告)日：1999-11-16

    申请号：US970363

    申请日：1997-11-14

    申请人： Robert James Colinas ,  Anne Catherine Walsh ,  David A. Lawrence

    发明人： Robert James Colinas ,  Anne Catherine Walsh ,  David A. Lawrence

    IPC分类号： C12N5/00 ,  C12N5/06 ,  G01N33/50 ,  C12N5/02 ,  G01N33/53 ,  G01N33/536

    CPC分类号： G01N33/5005

    摘要： The invention provides a method for separating target cells from a plurality of cells which is based on a reversible high affinity interaction between two molecules. The method comprises: forming a target cell/cell binding reagent/first molecule/second molecule/solid support complex, wherein the cell binding reagent is specific for target cells present within a plurality of cells and wherein the first molecule reversibly binds to the second molecule; removing non-target cells of the plurality of cells not attached to the solid support; and reversing the first molecule binding to the second molecule, thereby releasing the target cells as separate cells from the plurality of cells.

    摘要翻译： 本发明提供了一种基于两个分子之间的可逆高亲和力相互作用的从多个细胞中分离靶细胞的方法。 该方法包括：形成靶细胞/细胞结合试剂/第一分子/第二分子/固体支持复合物，其中所述细胞结合试剂对存在于多个细胞内的靶细胞是特异性的，并且其中所述第一分子可逆地结合到所述第二分子 ; 去除未连接到固体支持物上的多个细胞的非靶细胞; 并且使与第二分子结合的第一分子反转，从而从多个细胞释放作为分离的细胞的靶细胞。

    Immunoassays for glutathione and antibodies useful therein
    13.
    发明授权
    Immunoassays for glutathione and antibodies useful therein 失效
    免疫测定谷胱甘肽及其中有用的抗体

    公开(公告)号：US4859613A

    公开(公告)日：1989-08-22

    申请号：US929937

    申请日：1986-11-12

    申请人： David A. Lawrence

    发明人： David A. Lawrence

    IPC分类号： G01N33/574 ,  G01N33/68 ,  G01N33/74

    CPC分类号： G01N33/68 ,  G01N33/574 ,  G01N33/743 ,  Y10S435/948 ,  Y10S530/806 ,  Y10S530/808 ,  Y10S530/809 ,  Y10S530/868

    摘要： Monoclonal antibodies specifically immunologically reactive to thiol-modified glutathione and hybridoma cell lines producing such monoclonal antibodies. A method of producing antibodies specifically immunologically reactive with reduced glutathione by immunizing an animal using a thiol-modified glutathione, for example, a glutathione-N-ethylmaleimide-keyhole limpet hemocyanin conjugate. A method of utilizing the antibodies produced to quantitate the amount of reduced glutathione in a biological sample, to monitor glutathione-associated conditions, to monitor the formation of normal metabolic intermediates and to monitor the detoxification of foreign compounds.

    摘要翻译： 与硫醇修饰的谷胱甘肽和产生这种单克隆抗体的杂交瘤细胞系特异性免疫反应的单克隆抗体。 通过使用硫醇修饰的谷胱甘肽，例如谷胱甘肽-N-乙基马来酰亚胺 - 钥孔血蓝蛋白缀合物免疫动物，产生与还原型谷胱甘肽特异性免疫反应的抗体的方法。 一种利用产生的抗体来定量生物样品中还原型谷胱甘肽的量，监测谷胱甘肽相关病症，监测正常代谢中间体形成和监测外源化合物解毒的方法。

    ENGINE CYLINDER BANK-TO-BANK TORQUE IMBALANCE CORRECTION
    15.
    发明申请
    ENGINE CYLINDER BANK-TO-BANK TORQUE IMBALANCE CORRECTION 有权
    发动机缸体至银行扭矩不平衡校正

    公开(公告)号：US20160076471A1

    公开(公告)日：2016-03-17

    申请号：US14851033

    申请日：2015-09-11

    申请人： Ethan E. Bayer ,  Ayman Ismail ,  David A. Lawrence

    发明人： Ethan E. Bayer ,  Ayman Ismail ,  David A. Lawrence

    IPC分类号： F02D41/00 ,  F02D13/02 ,  F02B75/22 ,  F02D41/14

    CPC分类号： F02D41/0082 ,  F01L1/34 ,  F01L2013/111 ,  F01L2800/11 ,  F01L2820/041 ,  F01L2820/042 ,  F02B75/22 ,  F02D13/0226 ,  F02D13/0238 ,  F02D41/0085 ,  F02D41/1405 ,  F02D41/1443 ,  F02D41/1454 ,  F02D41/2451 ,  F02D41/2454 ,  F02D2041/001 ,  F02D2200/0411 ,  Y02T10/18

    摘要： An engine bank-to-bank airflow balancing technique includes calculating current and offset volumetric efficiencies of the engine and calculating a slope representing (i) a difference between the offset and current volumetric efficiencies and (ii) a difference between offset and current intake camshaft positions. Based on the respective exhaust gas oxygen concentrations, the technique involves calculating a volumetric efficiency correction corresponding to each cylinder bank and based on the slope and the volumetric efficiency corrections, calculating target intake camshaft position shifts. The technique further involves controlling offsets of the intake camshafts based on the target intake camshaft position shifts. After a predetermined number of target intake camshaft position shifts are determined and stored with respect to various combinations of engine speed and a ratio of intake manifold pressure to barometric pressure, final intake camshaft position shifts may be determined and utilized when determining the intake camshaft positions.

    摘要翻译： 发动机库对空气流平衡技术包括计算发动机的当前和偏移体积效率并计算斜率，其表示（i）偏移和当前体积效率之间的差异，以及（ii）偏置和当前进气凸轮轴位置之间的差异 。 基于相应的废气氧浓度，该技术涉及计算对应于每个气缸组的体积效率校正，并且基于斜率和体积效率校正，计算目标进气凸轮轴位置偏移。 该技术还涉及基于目标进气凸轮轴位置偏移来控制进气凸轮轴的偏移。 在相对于发动机转速和进气歧管压力与大气压力的比例的各种组合来确定和存储预定数量的目标进气凸轮轴位置偏移之后，可以在确定进气凸轮轴位置时确定和利用最终进气凸轮轴位置偏移。

    POLYPEPTIDES SHARING SEQUENCE IDENTITY WITH A FIBROBLAST GROWTH FACTOR POLYPEPTIDE AND NUCLEIC ACIDS ENCODING THE SAME
    19.
    发明申请
    POLYPEPTIDES SHARING SEQUENCE IDENTITY WITH A FIBROBLAST GROWTH FACTOR POLYPEPTIDE AND NUCLEIC ACIDS ENCODING THE SAME 审中-公开
    多肽与纤维蛋白原生长因子多肽和核酸共编序列序列识别

    公开(公告)号：US20090098603A1

    公开(公告)日：2009-04-16

    申请号：US12273334

    申请日：2008-11-18

    申请人： David BOTSTEIN ,  Audrey Goddard ,  Austin L. Gurney ,  Kenneth J. Hillan ,  David A. Lawrence ,  Margaret Ann Roy

    发明人： David BOTSTEIN ,  Audrey Goddard ,  Austin L. Gurney ,  Kenneth J. Hillan ,  David A. Lawrence ,  Margaret Ann Roy

    IPC分类号： C12P21/04 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06 ,  C07K14/00 ,  C12N1/21 ,  C12N1/19

    CPC分类号： C07K14/50 ,  C07K2317/24 ,  C07K2319/00

    摘要： The present invention is directed to novel polypeptides having homology to the PRO533 protein and to nucleic acid molecules encoding those polypeptides. Also provided herein are vectors and host cells comprising those nucleic acid sequences, chimeric polypeptide molecules comprising the polypeptides of the present invention fused to heterologous polypeptide sequences, antibodies which bind to the polypeptides of the present invention, and methods for producing the polypeptides of the present invention. The invention concerns compositions and methods for the diagnosis and treatment of neoplastic cell growth and proliferation in mammals, including humans. The invention is based on the identification of genes that are amplified in the genome of tumor cells. Such gene amplification is expected to be associated with the overexpression of the gene product and contribute to tumorigenesis and/or autocrine signaling. Accordingly, the proteins encoded by the amplified genes are believed to be useful targets for the diagnosis and/or treatment (including prevention) of certain cancers, and may act of predictors of the prognosis of tumor treatment. Furthermore, the compounds, compositions including antagonists and methods of the present invention are further expected to have therapeutic effect upon conditions characterized by FgF-19 modulation.

    摘要翻译： 本发明涉及与PRO533蛋白质和编码这些多肽的核酸分子具有同源性的新型多肽。 本文还提供了包含那些核酸序列的载体和宿主细胞，包含与异源多肽序列融合的本发明多肽的嵌合多肽分子，与本发明的多肽结合的抗体，以及本发明多肽的制备方法 发明。 本发明涉及用于诊断和治疗哺乳动物包括人类的肿瘤细胞生长和增殖的组合物和方法。 本发明基于在肿瘤细胞基因组中扩增的基因的鉴定。 预期这种基因扩增与基因产物的过度表达有关，并且有助于肿瘤发生和/或自分泌信号传导。 因此，由扩增的基因编码的蛋白质被认为是某些癌症的诊断和/或治疗（包括预防）的有用靶标，并且可以作为肿瘤治疗预后的预测因子。 此外，进一步预期本发明的化合物，包括拮抗剂和方法的组合物对于以FgF-19调节为特征的病症具有治疗作用。