公开(公告)号：US11787930B2

公开(公告)日：2023-10-17

申请号：US16721451

申请日：2019-12-19

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Ludovic Vincent ,  Andrew A. Brown ,  Mathieu Lessard-Viger

IPC: C08L33/08 ,  C12Q1/6869 ,  C12Q1/6853 ,  C08L33/26

CPC classification number: C08L33/08 ,  C08L33/26 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

公开(公告)号：US10955332B2

公开(公告)日：2021-03-23

申请号：US15848450

申请日：2017-12-20

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： James Tsay ,  Anmiv Prabhu ,  David Heiner ,  Edwin Li ,  Alexandre Richez ,  John M. Beierle ,  Kevan Samiee ,  Kristina Munoz ,  Leonid Malevanchik ,  Ludovic Vincent ,  Naiqian Zhan ,  Peyton Shieh ,  Robert Yang ,  Samantha Schmitt ,  Sang Park ,  Scott Bailey ,  Sean M. Ramirez ,  Sunmin Ahn ,  Valerie Uzzell ,  Wei Wei ,  Yuxiang Huang ,  Tyler Jamison Dill

IPC: G01N15/14 ,  C12Q1/6874 ,  B01L3/00 ,  C09D133/24 ,  C09D133/26 ,  B01J19/00 ,  G01N15/10

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

公开(公告)号：US20200224020A1

公开(公告)日：2020-07-16

申请号：US16721451

申请日：2019-12-19

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Ludovic Vincent ,  Andrew A. Brown ,  Mathieu Lessard-Viger

IPC: C08L33/08 ,  C08L33/26 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

公开(公告)号：US20230391997A1

公开(公告)日：2023-12-07

申请号：US18233276

申请日：2023-08-11

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Ludovic Vincent ,  Andrew A. Brown ,  Mathieu Lessard-Viger

IPC: C08L33/08 ,  C12Q1/6869 ,  C12Q1/6853 ,  C08L33/26

CPC classification number: C08L33/08 ,  C08L33/26 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

公开(公告)号：US11680292B2

公开(公告)日：2023-06-20

申请号：US17840569

申请日：2022-06-14

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16 ,  G03F7/00 ,  G03F7/004

CPC classification number: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0002 ,  G03F7/0046

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US11667969B2

公开(公告)日：2023-06-06

申请号：US15969562

申请日：2018-05-02

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED ,  ILLUMINA SINGAPORE PTE. LTD.

Inventor： Sean M. Ramirez ,  Brian D. Mather ,  Edwin Li ,  Sojeong Moon ,  Innsu Daniel Kim ,  Alexandre Richez ,  Ludovic Vincent ,  Xavier von Hatten ,  Hai Quang Tran ,  Maxwell Zimmerley ,  Julia Morrison ,  Gianluca Andrea Artioli ,  Krystal Sly ,  Hayden Black ,  Lewis J. Kraft ,  Hong Xie ,  Wei Wei ,  Ryan Sanford

IPC: B01L3/00 ,  C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  B01L3/502707 ,  C12Q1/6806 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/0887 ,  B01L2300/16 ,  B01L2300/165

Abstract: An example of a method includes providing a substrate with an exposed surface comprising a first chemical group, wherein the providing optionally comprises modifying the exposed surface of the substrate to incorporate the first chemical group; reacting the first chemical group with a first reactive group of a functionalized polymer molecule to form a functionalized polymer coating layer covalently bound to the exposed surface of the substrate; grafting a primer to the functionalized polymer coating layer by reacting the primer with a second reactive group of the functionalized polymer coating layer; and forming a water-soluble protective coating on the primer and the functionalized polymer coating layer. Examples of flow cells incorporating examples of the water-soluble protective coating are also disclosed herein.

公开(公告)号：US20230002822A1

公开(公告)日：2023-01-05

申请号：US17840569

申请日：2022-06-14

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20220231068A1

公开(公告)日：2022-07-21

申请号：US17574358

申请日：2022-01-12

Applicant: Illumina, Inc.

Inventor： Ludovic Vincent ,  Tara Bozorg-Grayeli ,  Arvin Emadi ,  Vanessa Montaño Machado

IPC: H01L27/146 ,  H01L23/00 ,  G01N33/543 ,  G01N27/414

Abstract: Provided herein include various examples of an apparatus, a sensor system and examples of a method for manufacturing aspects of an apparatus, a sensor system. The method may include forming bumps on a surface of one or more electrical contacts, where the one or more electrical contacts are accessible on an upper surface of a die, where the die is oriented on a substrate, and where the electrical contacts comprise bonding pads. The method may also include coupling one or more additional electrical contacts to the one or more electrical contacts, where the coupling comprises wire-bonding each additional electrical contact of the additional electrical contacts to one of the one or more electrical contacts accessible on the upper surface of the die, via a portion of the bumps on the surface of the one or more electrical contacts, thereby forming wire-bonded connections.

公开(公告)号：US11124829B2

公开(公告)日：2021-09-21

申请号：US16626452

申请日：2019-06-07

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/68 ,  G03F7/16 ,  C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/00 ,  G03F7/004

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20210139975A1

公开(公告)日：2021-05-13

申请号：US17139834

申请日：2020-12-31

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.