-
公开(公告)号：US10816505B2
公开(公告)日：2020-10-27
申请号：US16687610
申请日：2019-11-18
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： James A. Ball , Brian Reed
Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.
-
公开(公告)号：US09901887B2
公开(公告)日：2018-02-27
申请号：US15282283
申请日：2016-09-30
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , John Nobile , Brian Reed , Prasanna Thwar , Todd Roswech , John Andrew Sheridan
IPC: C12M1/00 , C12M3/00 , C12M1/34 , B01F3/08 , C12P19/34 , C12M1/26 , B01F15/00 , B04B11/02 , B01F5/04 , B01F13/00 , B01L3/00 , B01L7/00 , B04B5/04 , B04B7/02 , C12Q1/68
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
-
公开(公告)号：US09776146B2
公开(公告)日：2017-10-03
申请号：US14696642
申请日：2015-04-27
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan Schultz , John Nobile , Brian Reed , Prasanna Krishnan Thwar , Todd Roswech
IPC: B01F5/06 , C12M1/34 , C12M3/00 , B01F3/08 , B01F5/04 , B01F13/00 , B04B5/04 , B04B7/02 , B01L3/00
CPC classification number: B01F5/0602 , B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F2215/0037 , B01L3/502784 , B04B5/0414 , B04B7/02
Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
-
公开(公告)号：US20170157538A1
公开(公告)日：2017-06-08
申请号：US15375271
申请日：2016-12-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Reed , JAMES A. BALL
CPC classification number: B01D17/047 , B01D17/04 , B01D17/041 , C07H1/06 , C07H21/00 , C12Q1/6806 , C12Q2527/125 , C12Q2527/137 , C12Q2563/159
Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
-
公开(公告)号：US09533240B2
公开(公告)日：2017-01-03
申请号：US14536429
申请日：2014-11-07
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Reed , James A. Ball
CPC classification number: B01D17/047 , B01D17/04 , B01D17/041 , C07H1/06 , C07H21/00 , C12Q1/6806 , C12Q2527/125 , C12Q2527/137 , C12Q2563/159
Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
Abstract translation: 从乳液中回收珠粒载体的方法包括将表面活性剂水溶液供给到离心管中; 在离心管中的表面活性剂溶液上提供疏水性液体，其中表面活性剂水溶液的体积与疏水性液体的体积之比不大于0.5; 并且在离心时将乳液施加在疏水性液体上，所述乳液包含分散的水相，包括珠粒支持体，乳液破碎和分散相的材料优先分配至表面活性剂溶液。
-
Patent Agency Ranking
公开(公告)号：US20150314294A1
公开(公告)日：2015-11-05
申请号：US14799259
申请日：2015-07-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan SCHULTZ , John Nobile , Brian Reed , Prasanna Krishnan Thwar , Todd Roswech , John Andrew Sheridan
CPC classification number: B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F15/00 , B01F2215/0037 , B01L3/5021 , B01L3/502784 , B01L7/52 , B01L2200/026 , B01L2300/046 , B01L2300/06 , B01L2300/18 , B01L2300/1822 , B01L2300/1827 , B01L2300/1838 , B01L2400/0409 , B04B5/0414 , B04B7/02 , B04B11/02 , B04B2007/025 , C12M33/10 , C12P19/34 , C12Q1/686 , C12Q2523/32 , C12Q2563/159 , C12Q2565/629
Abstract: An automated template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, a thermal plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermal subsystem, in automated fashion. The thermal subsystem can treat an inverse emulsion passed therethrough. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
Abstract translation: 提供了一种自动化模板珠制备系统，包括膜基乳液生成子系统，热板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式将逆向乳液的连续流供应到热子系统。 热子系统可以处理通过其中的反相乳液。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。
-
公开(公告)号：US09150917B2
公开(公告)日：2015-10-06
申请号：US14057450
申请日：2013-10-18
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Todd Rearick , Jessica Lynn Reed , Jason Gioia , Devin Dressman , Nicholas Hapshe , Brian Reed , John Andrew Sheridan
IPC: C12Q1/68 , B01L3/00 , G01N33/50 , G01N33/543
CPC classification number: C12Q1/6874 , B01L3/5085 , B01L2200/0642 , B01L2200/0668 , B01L2300/0819 , B01L2300/0893 , C12Q1/6837 , G01N33/50 , G01N33/543
Abstract: Methods, compositions, systems, apparatus, and kits are provided for depositing samples onto surfaces. The samples can include one or more particles, and the surface can include one or more reaction chambers. In some embodiments, the depositing can include the use of companion particles in combination with sample particles.
Abstract translation: 提供了用于将样品沉积到表面上的方法，组合物，系统，装置和试剂盒。 样品可以包括一个或多个颗粒，并且该表面可以包括一个或多个反应室。 在一些实施方案中，沉积可以包括使用伴随颗粒与样品颗粒的组合。
-
公开(公告)号：US20150224457A1
公开(公告)日：2015-08-13
申请号：US14696642
申请日：2015-04-27
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan SCHULTZ , John Nobile , Brian Reed , Prasanna Krishnan Thwar , Todd Roswech
IPC: B01F5/06
CPC classification number: B01F5/0602 , B01F3/0807 , B01F5/0485 , B01F13/0059 , B01F2215/0037 , B01L3/502784 , B04B5/0414 , B04B7/02
Abstract: An automated on-touch template bead preparation system is provided and includes a membrane-based emulsion generation subsystems, an emulsion PCR (ePCR) thermocycling plate and subsystem, and a continuous centrifugation emulsion breaking and templated bead collection subsystem. The emulsion generation subsystem provides uniformity in the preparation of an inverse emulsion and may be used to create large or small volume inverse emulsions rapidly and reproducibly. An emulsion-generating device is provided that can supply a continuous stream of an inverse emulsion to a thermocycling subsystem, in automated fashion. The ePCR subsystem can continuously thermocycle an inverse emulsion passed therethrough and includes static temperature zones and a consumable thermocycling plate. The continuous centrifugation subsystem can continuously break a thermally cycled inverse emulsion and collect template beads formed in the aqueous microreactor droplets of the inverse emulsion.
Abstract translation: 提供了一种自动化的在线模板珠粒制备系统，包括膜基乳液生成子系统，乳液PCR（ePCR）热循环板和子系统，以及连续离心乳液破乳和模板珠收集子系统。 乳液生成子系统在制备反相乳液时提供均匀性，并可用于快速且可再现地产生大体积或小体积的反相乳液。 提供一种乳液生成装置，其能够以自动方式向逆循环子系统提供连续的反相乳液流。 ePCR子系统可以连续热循环通过的反相乳液，并包括静态温度区和可消耗的热循环板。 连续离心子系统可以连续地破坏热循环的反相乳液并收集形成在反相乳液的微量微反应器液滴中的模板珠粒。
-
公开(公告)号：US11229859B2
公开(公告)日：2022-01-25
申请号：US16786913
申请日：2020-02-10
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian Reed , James A. Ball
IPC: C07H1/06 , B01D17/04 , C07H21/00 , C12Q1/6806
Abstract: A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
-
公开(公告)号：US10527580B2
公开(公告)日：2020-01-07
申请号：US16045682
申请日：2018-07-25
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： James A. Ball , Brian Reed
Abstract: A sensor array includes a plurality of sensors. A sensor of the plurality of sensors has a sensor pad exposed at a surface of the sensor array. A method of treating the sensor array includes exposing at least the sensor pad to a wash solution including sulfonic acid and an organic solvent and rinsing the wash solution from the sensor pad.
-
-
-
-
-
-
-
-
-
Search Results
23
records
(took 0.037 seconds)
