公开(公告)号：US20200072826A1

公开(公告)日：2020-03-05

申请号：US16543389

申请日：2019-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Hua YU ,  Xiaoling YANG ,  Rui ZHENG ,  Chiu Tai Andrew WONG ,  Jeremy GRAY

IPC: G01N33/543 ,  C12Q1/6844

Abstract: The disclosure generally relates to systems, methods, and apparatuses for magnetic bead loading. An example embodiment of the disclosure relates to mixing magnetic beads with sequencing beads to form a solution. The solution containing both beads is injected onto a microchip having a plurality of microwells. The magnetic beads may have larger diameter than the microwell while the sequencing beads may have a smaller diameter, allowing them to enter and reside in the microwell. One or more magnets positioned under the microchip move back and forth across the microchip surface. The magnetic beads form a line and follow the movement of the magnets. During rounds of sweeping, the sequencing beads load into the respective wells. The magnets may be disengaged and the magnetic beads may be washed away after the sequencing beads are loaded.

公开(公告)号：US20190194719A1

公开(公告)日：2019-06-27

申请号：US16183722

申请日：2018-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Abraham ROSENBAUM ,  Collyn SEEGER ,  Jeremy GRAY ,  Hua YU

IPC: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6876

CPC classification number: C12Q1/686 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6853 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2600/16 ,  C12Q2525/197 ,  C12Q2535/122 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/519

Abstract: The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

公开(公告)号：US20190017107A1

公开(公告)日：2019-01-17

申请号：US16120007

申请日：2018-08-31

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David LIGHT ,  Wolfgang HINZ ,  Ronald CICERO ,  Christina INMAN ,  Paul KENNEY ,  Alexander MASTROIANNI ,  Roman ROZHKOV ,  Yufang WANG ,  Jeremy GRAY ,  Marc GLAZER ,  Dmitriy GREMYACHINSKIY

IPC: C12Q1/6837 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q1/6806

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q2523/101 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2547/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2523/301 ,  C12Q2523/32

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

公开(公告)号：US20170059517A1

公开(公告)日：2017-03-02

申请号：US15247288

申请日：2016-08-25

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James BUSTILLO ,  Jeremy GRAY ,  Jordan OWENS

IPC: G01N27/414 ,  H01L29/423

CPC classification number: G01N27/4148 ,  G01N27/4145 ,  H01L29/42324

Abstract: An apparatus includes a substrate; a gate structure disposed over the substrate and having an upper surface; a well structure disposed over the substrate and defining a well over the upper surface of the gate structure; a conductive layer disposed on the upper surface of the gate structure and at least partially extending along a wall of the well in the well structure; and a dielectric structure disposed over the well structure and defining an opening to the well.

Abstract translation: 一种装置包括：基板; 栅极结构，设置在所述衬底上并具有上表面; 设置在所述衬底上并且在所述栅极结构的上表面上限定阱的阱结构; 导电层，其设置在所述栅极结构的上表面上，并且至少部分地沿所述阱在所述阱结构中的壁延伸; 以及设置在所述阱结构上并且限定到所述阱的开口的电介质结构。

公开(公告)号：US20160032371A1

公开(公告)日：2016-02-04

申请号：US14774662

申请日：2014-03-13

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： David LIGHT ,  Wolfgang HINZ ,  Ronald L. CICERO ,  Christina E. INMAN ,  Paul M. KENNEY ,  Alexander MASTROIANNI ,  Roman ROZHKOV ,  Yufang WANG ,  Jeremy GRAY ,  Marc GLAZER ,  Dmitriy GREMYACHINSKIY

IPC: C12Q1/68

CPC classification number: C12Q1/6837 ,  C12Q1/6806 ,  C12Q1/6834 ,  C12Q1/6869 ,  C12Q2523/101 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2547/107 ,  C12Q2563/159 ,  C12Q2565/537 ,  C12Q2523/301 ,  C12Q2523/32

Abstract: A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

Abstract translation: 形成聚合物基质阵列的方法包括将水溶液施加到阱阵列的孔中。 该水溶液包括聚合物前体。 该方法还包括在井阵列上施加不混溶流体以将井阵列中的水溶液隔离并聚合在井阵列的孔中分离的聚合物前体以形成聚合物基质阵列。 一种装置包括传感器阵列，对应于传感器阵列的阱阵列，以及设置在阱阵列中的聚合物阵列阵列。