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    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    12.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开
    多重PCR的方法和组合

    公开(公告)号：US20150322490A1

    公开(公告)日：2015-11-12

    申请号：US14796400

    申请日：2015-07-10

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John LEAMON ,  Mark Andersen ,  Michael Thornton

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12Q1/6806 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

    Abstract translation: 本发明提供可用于样品中存在的一种或多种核酸的多重PCR的方法，组合物，试剂盒，系统和装置。 特别地，提供允许一个或多个靶序列的选择性扩增的各种靶特异性引物。 一方面，本发明涉及可用于选择性扩增与癌症或遗传性疾病相关的一种或多种靶序列的靶特异性引物。 在一些方面，使用所公开的方法，试剂盒，系统和装置获得的扩增靶序列可用于包括核酸测序在内的各种下游过程，并用于检测遗传变体的存在。

    CENTRIFUGE AND METHOD FOR LOADING A DEVICE
    15.
    发明申请
    CENTRIFUGE AND METHOD FOR LOADING A DEVICE 审中-公开
    用于加载设备的离散和方法

    公开(公告)号：US20160230214A1

    公开(公告)日：2016-08-11

    申请号：US15133428

    申请日：2016-04-20

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kristopher BARBEE ,  Ryan JONES ,  Sean MCCUSKER ,  Maximilian CARPINO ,  John LEAMON ,  Jonathan SCHULTZ

    IPC: C12Q1/68 ,  B04B5/04

    Abstract: An apparatus includes a robotic system providing movement in three orthogonal directions to an arm operable to receive a pipette tip and to facilitate movement of fluid into and out of the pipette tip. In addition, the apparatus can include a tray for receiving pipette tips, receptacles for receiving tubes, an apparatus for forming an emulsion, a device for forming particles that include copies of the polynucleotide, a device for enriching the particles and an apparatus for loading such particles onto a sensor array. The apparatus can further include receptacles for holding containers of reagent solutions. Optionally, the robot can include a gripper arm in addition to the pipette receiving arm.

    Abstract translation: 一种装置包括机器人系统，该机器人系统在三个正交方向上提供可操作以接收移液管尖端的臂并促进流体进入和移出移液管尖端的运动。 此外，该装置可以包括用于接收移液管吸头的托盘，用于接收管的容器，用于形成乳液的装置，用于形成包括多核苷酸的拷贝的颗粒的装置，用于富集颗粒的装置和用于加载颗粒的装置 颗粒到传感器阵列上。 该装置还可以包括容纳试剂溶液容器的容器。 可选地，除了移液管接收臂之外，机器人还可以包括夹持臂。

    MULTIPLEX TRANSCRIPTOME ANALYSIS
    16.
    发明申请
    MULTIPLEX TRANSCRIPTOME ANALYSIS 审中-公开

    公开(公告)号：US20160194694A1

    公开(公告)日：2016-07-07

    申请号：US15068466

    申请日：2016-03-11

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Kelli BRAMLETT ,  John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C12Q1/68

    CPC classification number: C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143 ,  C12Q2525/117

    Abstract: In some embodiments, the disclosure relates generally to methods, compositions, systems, apparatuses and kits comprising a multiplex nucleic acid amplification reaction that employs a plurality (e.g., hundreds, thousands, tens-of-thousands or hundreds-of-thousands) of different target-specific primer pairs that enable substantially simultaneous amplification of a plurality of different target sequences-of-interest in a single reaction mixture. In some embodiments, the multiplex nucleic acid amplification reaction generates a plurality of amplicons having sequences derived from a sample containing RNA or DNA, including whole transcriptome or genomic samples. In some embodiments, the sequences and abundances of at least some of the plurality of amplicons are characterized, optionally simultaneously or through a single assay, by suitable detection methods, including sequencing or other procedures known in the art.

    SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING
    19.
    发明申请
    SCAFFOLDED NUCLEIC ACID POLYMER PARTICLES AND METHODS OF MAKING AND USING 审中-公开
    SCAFFEDED核酸聚合物颗粒及其制备和使用方法

    公开(公告)号：US20140128269A1

    公开(公告)日：2014-05-08

    申请号：US14044712

    申请日：2013-10-02

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： Wolfgang HINZ ,  John LEAMON ,  David LIGHT ,  Jonathan M. ROTHBERG

    IPC: C12Q1/68

    CPC classification number: B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12N15/1093 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

    Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

    Abstract translation: 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。

    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR
    20.
    发明申请
    METHODS AND COMPOSITIONS FOR MULTIPLEX PCR 审中-公开

    公开(公告)号：US20130059762A1

    公开(公告)日：2013-03-07

    申请号：US13679706

    申请日：2012-11-16

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： John LEAMON ,  Mark ANDERSEN ,  Michael THORNTON

    IPC: C40B50/06

    CPC classification number: C12Q1/6855 ,  C12Q1/6806 ,  C12Q2521/307 ,  C12Q2525/101 ,  C12Q2525/191 ,  C12Q2537/143

    Abstract: The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various target-specific primers are provided that allow for the selective amplification of one or more target sequences. In one aspect, the invention relates to target-specific primers useful for the selective amplification of one or more target sequences associated with cancer or inherited disease. In some aspects, amplified target sequences obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing and used to detect the presence of genetic variants.

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