公开(公告)号：US20170247504A1

公开(公告)日：2017-08-31

申请号：US15491650

申请日：2015-08-14

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Xin WANG ,  Hee Chol KANG ,  Aimei CHEN ,  Yexin WU ,  Yi-Zhen HU ,  Robert AGGELER ,  Quentin LOW

IPC: C08G61/12 ,  G01N33/545 ,  G01N33/58 ,  G01N33/533 ,  C07F7/08 ,  C09K11/06

CPC classification number: C08G61/123 ,  C07F7/0816 ,  C07F7/083 ,  C08G2261/122 ,  C08G2261/124 ,  C08G2261/1424 ,  C08G2261/145 ,  C08G2261/1644 ,  C08G2261/1646 ,  C08G2261/3142 ,  C08G2261/3244 ,  C08G2261/334 ,  C08G2261/344 ,  C08G2261/411 ,  C08G2261/522 ,  C08G2261/5222 ,  C08G2261/72 ,  C08G2261/94 ,  C08L65/00 ,  C09K11/06 ,  C09K2211/1416 ,  C09K2211/1491 ,  G01N33/533 ,  G01N33/545 ,  G01N33/582 ,  H01L51/0039 ,  H01L51/0043 ,  H01L51/0094

Abstract: Water-soluble, conjugated polymers containing one or more dibenzosilole monomer residues, as well as compositions, kits, and methods of making and using such polymers are disclosed. Also disclosed are dibenzosilole derivatives substituted with one or more water-solubilizing groups, and methods of making and using such derivatives to prepare water-soluble dibenzosilole polymers.

公开(公告)号：US20160281095A1

公开(公告)日：2016-09-29

申请号：US15178821

申请日：2016-06-10

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Anilkumar KORE ,  Shanmugasundaram MUTHIAN ,  Kyle GEE

IPC: C12N15/67 ,  C07C323/60 ,  C12P19/34 ,  A61K39/39 ,  A61K39/12 ,  C12N7/00 ,  C12N15/11 ,  C07D213/82

CPC classification number: C12N15/67 ,  A61K31/7084 ,  A61K31/7088 ,  A61K31/7115 ,  A61K31/712 ,  A61K39/12 ,  A61K39/39 ,  A61K48/00 ,  A61K2039/53 ,  A61K2039/55511 ,  C07C247/04 ,  C07C323/60 ,  C07D213/82 ,  C07H1/00 ,  C07H1/02 ,  C07H1/04 ,  C07H19/207 ,  C07H21/02 ,  C12N7/00 ,  C12N15/11 ,  C12N2770/36134 ,  C12N2770/36171 ,  C12P19/34 ,  C12Q1/68

Abstract: Alkynyl-derivatized cap analogs, alkynyl-modified capped RNA, 1,4-disubstituted triazole-derivatized capped RNA, methods of preparation, methods of isolation, and uses thereof are provided. The “click” modification facilitates detection and isolation of capped RNAs and the 1,4-disubstituted triazole derivatives formed by the “click” reaction are useful for producing RNA transcripts and encoded protein.

公开(公告)号：US20140038856A1

公开(公告)日：2014-02-06

申请号：US13890137

申请日：2013-05-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Aleksey RUKAVISHNIKOV ,  Courtenay HART ,  Richard HAUGLAND ,  Wai-Yee LEUNG ,  Wayne PATTON ,  Zhenjun DIWU

IPC: G01N33/58

CPC classification number: G01N33/52 ,  A61K49/0021 ,  A61K49/0039 ,  A61K49/0041 ,  A61K49/0052 ,  C07D311/12 ,  C07D311/16 ,  C07D311/18 ,  C07F5/022 ,  G01N33/533 ,  G01N33/582

Abstract: The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment.

Abstract translation: 本发明提供了通过能够选择性地结合亲和标签来促进融合蛋白的检测和标记的方法和荧光化合物。 荧光化合物具有通式A（B）n，其中A是荧光团，B是作为带电化学部分的结合结构域，其蛋白质或片段，n是1-6的整数，条件是 蛋白质或其片段不是抗体或从抗体产生。 本发明提供用于检测和标记含有多组氨酸亲和标签的融合蛋白的特异性荧光化合物和方法。 这些化合物具有通式A（L）m（B）n，其中A是荧光团，L是连接体，B是乙酸结合域，m是1至4的整数，n是1至 乙酸基团与亲和标签的带正电荷的组氨酸残基直接相互作用，以有效地标记和检测当存在于酸性或中性环境中时含有这种亲和标签的融合蛋白。

公开(公告)号：US20210324198A1

公开(公告)日：2021-10-21

申请号：US17150247

申请日：2019-08-08

Applicant: Life Technologies Corporation

Inventor： Jongtae YANG ,  Xin WANG ,  Yi-Zhen HU ,  Kyle GEE ,  Aimei CHEN ,  Scott BENSON ,  Brian EVANS ,  Aleksey RUKAVISHNIKOV

IPC: C09B11/28 ,  C07F7/08 ,  C07F9/6561 ,  C12Q1/6816 ,  C12Q1/6869 ,  G01N33/58

Abstract: Silicon-substituted rhodamine compounds are disclosed herein. Also described herein are SiR dyes comprising at least one vinyl group attached to the Si atom (10 position) of the SiR dye. Derivatives, functionalized versions, conjugates, kits, related synthetic methods and uses of SiR compounds also are provided. Silicon-rhodamine (SiR) dyes can provide bright fluorescence at far red wavelengths and exhibit good photostability. The compounds described herein can be useful for fluorescent labeling and detection of biological samples.

公开(公告)号：US20200330610A1

公开(公告)日：2020-10-22

申请号：US16882271

申请日：2020-05-22

Applicant: Life Technologies Corporation ,  Pierce Biotechnology, Inc.

Inventor： Surbhi DESAI ,  Marie Christine NLEND ,  Kyle GEE ,  Matthew BAKER ,  Robert AGGELER ,  Scott SWEENEY ,  Aleksey RUKAVISHNIKOV ,  Shih-Jung HUANG

IPC: A61K49/00 ,  G01N33/58 ,  A61K47/10 ,  A61K47/18 ,  G01N33/533 ,  G01N33/68

Abstract: This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.

公开(公告)号：US20190309341A1

公开(公告)日：2019-10-10

申请号：US16306609

申请日：2017-06-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Wenjun ZHOU ,  Shih-Jung HUANG ,  Monica TOMASZEWSKI

IPC: C12Q1/37 ,  G01N33/58 ,  C07K5/113 ,  C07K5/103 ,  G01N21/64

Abstract: Provided herein are compounds, enzyme substrates, compositions, kits, uses, and methods for detecting the presence or absence of a caspase enzyme, measuring the activity of a caspase enzyme, or detecting the presence or absence of apoptosis. The detection or measurement can occur through intracellular cleavage of a compound or enzyme substrate, which can lead to an increase in fluorescence, e.g., in the violet or red channel, through liberation of a nucleic acid binding dye from a peptide, such as liberation of a DNA-binding dye from a negatively charged peptide comprising a sequence recognized and cleaved by a caspase

公开(公告)号：US20190187155A1

公开(公告)日：2019-06-20

申请号：US16324769

申请日：2017-08-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Daniel BEACHAM ,  Kyle GEE ,  Aleksey RUKAVISHNIKOV

IPC: G01N33/68 ,  C07D493/10 ,  G01N33/84

CPC classification number: G01N33/6872 ,  C07C201/00 ,  C07C229/18 ,  C07C251/30 ,  C07C2603/24 ,  C07D311/90 ,  C07D493/10 ,  C09B1/00 ,  C09B11/24 ,  G01N33/84 ,  G01N2333/705

Abstract: Compositions and methods for detecting the activity of an ion channel in a cell are described. The methods include providing a loading buffer solution to the cell, where the loading buffer includes a thallium ion indicator and optionally chloride ions, and providing a stimulus buffer that includes thallium ions to the cell. Providing the stimulus buffer can cause thallium ion influx into or efflux out of the cell through the ion channel. After providing the stimulus buffer, a change in at least one optical property of the thallium ion indicator is detected in response to thallium influx or efflux, thereby detecting the activity of the ion channel.

公开(公告)号：US20180092993A1

公开(公告)日：2018-04-05

申请号：US15693772

申请日：2017-09-01

Applicant: Life Technologies Corporation ,  Pierce Biotechnology, Inc.

Inventor： Surbhi DESAI ,  Marie Christine NLEND ,  Kyle GEE ,  Matthew BAKER ,  Robert AGGELER ,  Scott SWEENEY ,  Aleksey RUKAVISHNIKOV ,  Shih-Jung HUANG

IPC: A61K49/00 ,  A61K47/18 ,  A61K47/10 ,  G01N33/533 ,  G01N33/68

CPC classification number: A61K49/0015 ,  A61K47/10 ,  A61K47/18 ,  A61K49/0058 ,  G01N33/533 ,  G01N33/582 ,  G01N33/68

Abstract: This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.

公开(公告)号：US20170145214A1

公开(公告)日：2017-05-25

申请号：US15423890

申请日：2017-02-03

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Upinder SINGH ,  Aleksey RUKAVISHNIKOV ,  Daniel BEACHAM ,  Shih-Jung HUANG ,  Michael JANES ,  Wenjun ZHOU

IPC: C09B11/24 ,  G01N33/84 ,  G01N33/58

CPC classification number: C09B11/24 ,  A61K49/0041 ,  C07D311/90 ,  G01N33/5005 ,  G01N33/582 ,  G01N33/84

Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.

公开(公告)号：US20160334393A1

公开(公告)日：2016-11-17

申请号：US15221230

申请日：2016-07-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kyle GEE ,  Michael JANES ,  Iain JOHNSON ,  Hee Chol KANG

IPC: G01N33/52 ,  G01N33/58 ,  C07F9/54 ,  C07D491/16 ,  C07K5/02

CPC classification number: G01N33/52 ,  C07D491/06 ,  C07D491/16 ,  C07F9/5442 ,  C07K5/0215 ,  G01N33/582 ,  G01N2333/4701 ,  Y10T436/17 ,  Y10T436/182

Abstract: Fluorescent quinolizinocoumarin compounds substituted with electrophilic reactive groups that bind thiol compounds are described. The compounds are useful in detecting oxidative stress and processes associated therewith in live cells.