公开(公告)号：US20160054327A1

公开(公告)日：2016-02-25

申请号：US14688986

申请日：2015-04-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Diller ,  Bethany Sutton ,  George Hanson ,  Timothy Updyke

IPC: G01N33/58

CPC classification number: G01N33/582 ,  B33Y80/00 ,  G01N33/6803 ,  G01N2550/00

Abstract: The present invention provides methods and compositions for labeling, separating and analyzing proteins, particularly a specific protein of interest within a cell lysate or in a mixture of proteins. The proteins are labeled with an amine reactive or thiol reactive fluorescent dye, or an amine reactive fluorogenic reagent that becomes fluorescent upon reacting to amine groups located on the protein. Following the labeling step, the proteins within the mixture can be separated and analyzed. In a further embodiment, a tag binding fluorogenic reagent that can bind to a tag on a tagged protein is added to specifically label the protein of interest.

公开(公告)号：US09097663B2

公开(公告)日：2015-08-04

申请号：US13717650

申请日：2012-12-17

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Timothy Updyke ,  Paul Predki ,  Evangeline Gonzalez ,  Randall Lowe ,  Tad Simons

IPC: G01N27/447

CPC classification number: G01N27/44721 ,  G01N27/44778

Abstract: The present teachings provide methods, devices, systems, and materials for performing electrophoresis in an automated fashion. The electrophoresis system may simultaneously image gel during an electrophoresis run. In some embodiments, the electrophoresis system may analyze an imaged gel during or after electrophoresis. The device may comprise a gel processing system, a gel illumination system, an image capture system, and an image analysis all housed within a housing.

Abstract translation: 本教导提供了以自动方式进行电泳的方法，装置，系统和材料。 电泳系统可以在电泳过程中同时成像凝胶。 在一些实施方案中，电泳系统可以在电泳期间或之后分析成像的凝胶。 该装置可以包括凝胶加工系统，凝胶照明系统，图像捕获系统和全部容纳在壳体内的图像分析。

公开(公告)号：US09808799B2

公开(公告)日：2017-11-07

申请号：US14462640

申请日：2014-08-19

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Joseph Amshey ,  Daniel Bezdek ,  Espir Kahatt ,  Alexander Khorlin ,  Lance Parsons ,  Todd Peterson ,  Timothy Powers ,  Michael Thacker ,  Timothy Updyke ,  Kornelija Zgonc

IPC: B01L9/00 ,  B01L3/00 ,  B01L7/00 ,  G01N35/00 ,  G01N35/10

CPC classification number: B01L3/502715 ,  B01L3/50273 ,  B01L7/00 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0681 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/123 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/0605 ,  B01L2400/0655 ,  G01N2035/00306 ,  G01N2035/1037 ,  Y10T436/2575

Abstract: The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like.

公开(公告)号：USD795449S1

公开(公告)日：2017-08-22

申请号：US29511203

申请日：2014-12-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Designer： Jennifer Miller ,  Paul Predki ,  Timothy Updyke ,  Itay Sela ,  Shimon Spector

公开(公告)号：US20160025676A1

公开(公告)日：2016-01-28

申请号：US14728471

申请日：2015-06-02

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Thomas Beardslee ,  Timothy Updyke

IPC: G01N27/447

CPC classification number: G01N27/44756 ,  C07K1/26 ,  C08J3/075 ,  C08J2333/26 ,  C08K5/0008 ,  C08L33/26 ,  G01N27/44747

Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

Abstract translation: 提供凝胶，如聚丙烯酰胺凝胶，其包括在聚集凝胶中的线性聚丙烯酰胺。 在堆垛机中包括线性聚丙烯酰胺的天然凝胶可用于分离生物分子复合物，例如蛋白质复合物。 还提供了其中前板和后板之间的间隙宽度在盒的上边缘处不变化大于5％的凝胶盒。 凝胶盒可以用于天然凝胶上的蛋白质和蛋白质复合物的电泳分离，例如在堆积器中包括线性聚丙烯酰胺的天然凝胶。 天然凝胶可以具有多个用于电泳至少一个样品和/或至少一个分子量标准的孔。

公开(公告)号：US09057694B2

公开(公告)日：2015-06-16

申请号：US13928137

申请日：2013-06-26

Applicant: Life Technologies Corporation

Inventor： Timothy Updyke ,  Thomas Beardslee

IPC: G01N27/447 ,  C07K1/26 ,  C08J3/075 ,  C08K5/00 ,  C08L33/26

CPC classification number: G01N27/44756 ,  C07K1/26 ,  C08J3/075 ,  C08J2333/26 ,  C08K5/0008 ,  C08L33/26 ,  G01N27/44747

Abstract: Gels, such as polyacrylamide gels, are provided that include linear polyacrylamide in the stacking gel. Native gels that include linear polyacrylamide in the stacker can be used to separate biomolecular complexes, such as protein complexes. Gel cassettes in which the gap width between front and back plates does not vary by more than 5% at the upper edge of the cassette are also provided. The gel cassettes can be used for electrophoretic separation of proteins and protein complexes on native gels, such as native gels that include linear polyacrylamide in the stacker. The native gels can have multiple wells for electrophoresing at least one sample and/or at least one molecular weight standard.

Abstract translation: 提供凝胶，如聚丙烯酰胺凝胶，其包括在聚集凝胶中的线性聚丙烯酰胺。 在堆垛机中包括线性聚丙烯酰胺的天然凝胶可用于分离生物分子复合物，例如蛋白质复合物。 还提供了其中前板和后板之间的间隙宽度在盒的上边缘处不变化大于5％的凝胶盒。 凝胶盒可以用于天然凝胶上的蛋白质和蛋白质复合物的电泳分离，例如在堆积器中包括线性聚丙烯酰胺的天然凝胶。 天然凝胶可以具有多个用于电泳至少一个样品和/或至少一个分子量标准的孔。