公开(公告)号：US07738945B2

公开(公告)日：2010-06-15

申请号：US10716744

申请日：2003-11-18

申请人： Mark E. Fauver ,  J. Richard Rahn ,  Eric J. Seibel ,  Alan C. Nelson

发明人： Mark E. Fauver ,  J. Richard Rahn ,  Eric J. Seibel ,  Alan C. Nelson

IPC分类号： A61B5/05

CPC分类号： G01N15/1475 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/05 ,  G01N21/4795 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/1447 ,  G01N2021/0346 ,  G01N2021/1787 ,  G01N2201/0813 ,  G01N2223/612 ,  G06T11/006 ,  G06T2211/421

摘要： A system for optical imaging of a thick specimen that permits rapid acquisition of data necessary for tomographic reconstruction of the three-dimensional (3D) image. One method involves the scanning of the focal plane of an imaging system and integrating the range of focal planes onto a detector. The focal plane of an optical imaging system is scanned along the axis perpendicular to said plane through the thickness of a specimen during a single detector exposure. Secondly, methods for reducing light scatter when using illumination point sources are presented. Both approaches yield shadowgrams. This process is repeated from multiple perspectives, either in series using a single illumination/detection subsystem, or in parallel using several illumination/detection subsystems. A set of pseudo-projections is generated, which are input to a three dimensional tomographic image reconstruction algorithm.

摘要翻译： 一种用于光学成像厚样本的系统，其允许快速获取三维（3D）图像的层析成像重建所需的数据。 一种方法涉及扫描成像系统的焦平面并将焦平面的范围集成到检测器上。 在单个检测器曝光期间，光学成像系统的焦平面沿着垂直于所述平面的轴线扫描样本的厚度。 其次，提出了使用照明点源时减少光散射的方法。 两种方法产生阴影图。 该过程从多个角度重复，使用单个照明/检测子系统串联使用，或者使用几个照明/检测子系统并行重复。 生成一组伪投影，其被输入到三维断层图像重建算法。

公开(公告)号：US08143600B2

公开(公告)日：2012-03-27

申请号：US12032959

申请日：2008-02-18

申请人： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

发明人： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

IPC分类号： G01N21/64

CPC分类号： G01N21/4795 ,  G01N15/1436 ,  G01N2015/1445 ,  G01N2021/1787 ,  G06T11/006

摘要： A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

摘要翻译： 用于光学断层摄影系统中的细胞的3D成像的方法包括相对于显微镜物镜移动生物体以呈现不同的视角。 用具有限制在150nm和390nm之间的波长的光谱带宽的辐射照射该生物物体。 利用摄像机从多个不同的视角检测透过生物物体和显微镜物镜的辐射。 形成来自感测辐射的生物物体的多个伪投影，并重建多个伪投影以形成该细胞的3D图像。

公开(公告)号：US20090208072A1

公开(公告)日：2009-08-20

申请号：US12032959

申请日：2008-02-18

申请人： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

发明人： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

IPC分类号： G06K9/00

CPC分类号： G01N21/4795 ,  G01N15/1436 ,  G01N2015/1445 ,  G01N2021/1787 ,  G06T11/006

摘要： A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

摘要翻译： 用于光学断层摄影系统中的细胞的3D成像的方法包括相对于显微镜物镜移动生物体以呈现不同的视角。 用具有限制在150nm和390nm之间的波长的光谱带宽的辐射照射该生物物体。 利用摄像机从多个不同的视角检测透过生物物体和显微镜物镜的辐射。 形成来自感测辐射的生物物体的多个伪投影，并重建多个伪投影以形成该细胞的3D图像。

公开(公告)号：US08368035B2

公开(公告)日：2013-02-05

申请号：US13402149

申请日：2012-02-22

申请人： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

发明人： Eric J. Seibel ,  Alan C. Nelson ,  Mark E. Fauver ,  J. Richard Rahn

IPC分类号： G01N21/64

CPC分类号： G01N21/4795 ,  G01N15/1436 ,  G01N2015/1445 ,  G01N2021/1787 ,  G06T11/006

摘要： A method for 3D imaging of cells in an optical tomography system includes moving a biological object relatively to a microscope objective to present varying angles of view. The biological object is illuminated with radiation having a spectral bandwidth limited to wavelengths between 150 nm and 390 nm. Radiation transmitted through the biological object and the microscope objective is sensed with a camera from a plurality of differing view angles. A plurality of pseudoprojections of the biological object from the sensed radiation is formed and the plurality of pseudoprojections is reconstructed to form a 3D image of the cell.

摘要翻译： 用于光学断层摄影系统中的细胞的3D成像的方法包括相对于显微镜物镜移动生物体以呈现不同的视角。 用具有限制在150nm和390nm之间的波长的光谱带宽的辐射照射该生物物体。 利用摄像机从多个不同的视角检测透过生物物体和显微镜物镜的辐射。 形成来自感测辐射的生物物体的多个伪投影，并重建多个伪投影以形成该细胞的3D图像。

公开(公告)号：US07555333B2

公开(公告)日：2009-06-30

申请号：US10655482

申请日：2003-09-04

申请人： Wei-Chi Wang ,  Eric J. Seibel ,  Per G. Reinhall ,  Mark E. Fauver ,  Chris M. Brown

发明人： Wei-Chi Wang ,  Eric J. Seibel ,  Per G. Reinhall ,  Mark E. Fauver ,  Chris M. Brown

IPC分类号： A61B6/00

CPC分类号： A61B5/0062 ,  A61B1/00048 ,  A61B1/0008 ,  A61B1/00165 ,  A61B1/00167 ,  A61B1/00172 ,  A61B1/00183 ,  A61B1/00193 ,  A61B1/0051 ,  A61B1/043 ,  A61B1/063 ,  A61B1/0638 ,  A61B5/0066 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/0086 ,  A61B5/1076 ,  A61N5/0601 ,  A61N5/062 ,  G02B6/262 ,  G02B6/32 ,  G02B6/3502 ,  G02B6/3566 ,  G02B6/357 ,  G02B6/3572 ,  G02B6/3578 ,  G02B23/2484 ,  G02B26/0875 ,  G02B26/10 ,  G02B26/101

摘要： An apparatus and method for providing image acquisition and/or image display in a limited region of interest (ROI). The apparatus comprises a micro electro-mechanical system (MEMS), preferably integrating a light source, a cantilever, a lens, an actuator, a light detector, and a position sensor. The light source provides light for illuminating the ROI, displaying an image, providing a therapy, and/or performing other functions. The cantilever comprises a resin waveguide with a fixed end attached to a substrate that supports many or all other components. A free end of the cantilever is released from the substrate during fabrication and includes the lens. The actuator scans the free end in orthogonal directions to illuminate the ROI or display an image. The position sensors detect the position of the free end for control. The light detector receives light backscattered from the ROI separate from, or at the fixed end the cantilever.

摘要翻译： 一种用于在有限的感兴趣区域（ROI）中提供图像获取和/或图像显示的装置和方法。 该装置包括微机电系统（MEMS），优选地集成光源，悬臂，透镜，致动器，光检测器和位置传感器。 光源提供用于照亮ROI，显示图像，提供治疗和/或执行其他功能的光。 悬臂包括具有固定端连接到支撑多个或所有其它部件的基板的树脂波导。 在制造过程中，悬臂的自由端从基板释放并且包括透镜。 致动器沿正交方向扫描自由端以照亮ROI或显示图像。 位置传感器检测自由端的位置进行控制。 光检测器接收来自ROI的反向散射光，或者在固定端处悬臂。

公开(公告)号：US06959130B2

公开(公告)日：2005-10-25

申请号：US11074241

申请日：2005-03-02

申请人： Mark E. Fauver ,  Eric J. Seibel ,  Janet Crossman-Bosworth

发明人： Mark E. Fauver ,  Eric J. Seibel ,  Janet Crossman-Bosworth

IPC分类号： A61B19/00 ,  G02B6/00 ,  G02B6/25 ,  G02B6/255 ,  G02B6/26 ,  G02B6/32 ,  G02B26/08 ,  G02B26/10 ,  C25F3/00

CPC分类号： G02B26/0875 ,  A61B2090/3614 ,  G02B6/25 ,  G02B6/2552 ,  G02B6/262 ,  G02B6/32 ,  G02B26/101 ,  G02B2006/0098

摘要： An optical fiber having a reduced cross-sectional region adjacent to its distal end, which is fused to an optical component, is vibrated, rotating the optical component to scan a region. The optical component has a back focal point that is substantially coincident with an effective light source of the optical fiber, so that the light emanating from the optical component is either substantially collimated or convergent. The optical component is either a ball lens, a drum lens, a graded index lens, or a diffractive optical element. A vibratory node is also made substantially coincident with the back focal point of the optical component, producing a compact scanner with extensive field of view. The optical fiber is preferably reduced in cross-sectional area after the optical component is fused to the optical fiber, by immersion in a three-layer etch apparatus having an etch-stop layer, an etch layer, and a solvent layer.

摘要翻译： 具有与其远端相邻的与光学部件熔合的横截面减小的光纤振动，旋转光学部件以扫描区域。 光学部件具有与光纤的有效光源基本一致的后焦点，使得从光学部件发出的光基本上准直或收敛。 光学部件是球形透镜，鼓形透镜，渐变折射率透镜或衍射光学元件。 振动节点也基本上与光学部件的后焦点重合，产生具有广泛视场的紧凑型扫描仪。 光学元件通过浸入具有蚀刻停止层，蚀刻层和溶剂层的三层蚀刻装置中而在光学元件熔合到光纤之后，优选地减小横截面积。

公开(公告)号：US20090235396A1

公开(公告)日：2009-09-17

申请号：US12434129

申请日：2009-05-01

申请人： Wei-Chih Wang ,  Eric J. Seibel ,  Per G. Reinhall ,  Mark E. Fauver ,  Chris M. Brown

发明人： Wei-Chih Wang ,  Eric J. Seibel ,  Per G. Reinhall ,  Mark E. Fauver ,  Chris M. Brown

IPC分类号： G01N13/10

CPC分类号： A61B5/0062 ,  A61B1/00048 ,  A61B1/0008 ,  A61B1/00165 ,  A61B1/00167 ,  A61B1/00172 ,  A61B1/00183 ,  A61B1/00193 ,  A61B1/0051 ,  A61B1/043 ,  A61B1/063 ,  A61B1/0638 ,  A61B5/0066 ,  A61B5/0068 ,  A61B5/0071 ,  A61B5/0075 ,  A61B5/0084 ,  A61B5/0086 ,  A61B5/1076 ,  A61N5/0601 ,  A61N5/062 ,  G02B6/262 ,  G02B6/32 ,  G02B6/3502 ,  G02B6/3566 ,  G02B6/357 ,  G02B6/3572 ,  G02B6/3578 ,  G02B23/2484 ,  G02B26/0875 ,  G02B26/10 ,  G02B26/101

摘要： An apparatus and method for providing image acquisition and/or image display in a limited region of interest (ROI). The apparatus comprises a micro-electro-mechanical system (MEMS), preferably integrating a light source, a cantilever, a lens, an actuator, a light detector, and a position sensor. The light source provides light for illuminating the ROI, displaying an image, providing a therapy, and/or performing other functions. The cantilever comprises a resin waveguide with a fixed end attached to a substrate that supports many or all other components. A free end of the cantilever is released from the substrate during fabrication and includes the lens. The actuator scans the free end in orthogonal directions to illuminate the ROI or display an image. The position sensors detect the position of the free end for control. The light detector receives light backscattered from the ROI separate from, or at the fixed end of the cantilever.

摘要翻译： 一种用于在有限的感兴趣区域（ROI）中提供图像获取和/或图像显示的装置和方法。 该装置包括微机电系统（MEMS），优选地集成光源，悬臂，透镜，致动器，光检测器和位置传感器。 光源提供用于照亮ROI，显示图像，提供治疗和/或执行其他功能的光。 悬臂包括具有固定端连接到支撑多个或所有其它部件的基板的树脂波导。 在制造过程中，悬臂的自由端从基板释放并且包括透镜。 致动器沿正交方向扫描自由端以照亮ROI或显示图像。 位置传感器检测自由端的位置进行控制。 光检测器从与悬臂的固定端分离或在悬臂的固定端接收来自ROI的反向散射光。

公开(公告)号：US07068878B2

公开(公告)日：2006-06-27

申请号：US10763896

申请日：2004-01-23

申请人： Janet Crossman-Bosworth ,  Eric J. Seibel ,  Mark E. Fauver

发明人： Janet Crossman-Bosworth ,  Eric J. Seibel ,  Mark E. Fauver

IPC分类号： G02B6/26

CPC分类号： G02B26/0875 ,  A61B2090/3614 ,  G02B6/25 ,  G02B6/2552 ,  G02B6/262 ,  G02B6/32 ,  G02B26/101 ,  G02B2006/0098

摘要： An optical fiber having a reduced cross-sectional region adjacent to its distal end, which is fused to an optical component, is vibrated, rotating the optical component to scan a region. The optical component has a back focal point that is substantially coincident with an effective light source of the optical fiber, so that the light emanating from the optical component is either substantially collimated or convergent. The optical component is either a ball lens, a drum lens, a graded index lens, or a diffractive optical element. A vibratory node is also made substantially coincident with the back focal point of the optical component, producing a compact scanner with extensive field of view. The optical fiber is preferably reduced in cross-sectional area after the optical component is fused to the optical fiber, by immersion in a three-layer etch apparatus having an etch-stop layer, an etch layer, and a solvent layer.

摘要翻译： 具有与其远端相邻的与光学部件熔合的横截面减小的光纤振动，旋转光学部件以扫描区域。 光学部件具有与光纤的有效光源基本一致的后焦点，使得从光学部件发出的光基本上准直或收敛。 光学部件是球形透镜，鼓形透镜，渐变折射率透镜或衍射光学元件。 振动节点也基本上与光学部件的后焦点重合，产生具有广泛视场的紧凑型扫描仪。 光学元件通过浸入具有蚀刻停止层，蚀刻层和溶剂层的三层蚀刻装置中而在光学元件熔合到光纤之后，优选地减小横截面积。

公开(公告)号：US06991738B1

公开(公告)日：2006-01-31

申请号：US10964513

申请日：2004-10-13

申请人： Mark E. Fauver ,  Alan C. Nelson

发明人： Mark E. Fauver ,  Alan C. Nelson

IPC分类号： B01D21/26 ,  B01D37/02

CPC分类号： B04B5/0442 ,  B04B1/02 ,  B04B15/08 ,  B04B2005/0478 ,  B04B2005/0485 ,  G01N1/2813 ,  G01N2001/2846

摘要： A process includes coating a cylinder having an inner wall and a cylinder axis with a gel coating on the inner wall. Then a specimen mixture including solvent is made to flow through the cylinder while the cylinder is being continuously rotated. The specimen mixture is initially directed to flow along the cylinder axis and such that specimen particles from the specimen mixture are accelerated off the cylinder axis toward the inner wall, so as to form a film of specimen particles embedded into the gel coating.

摘要翻译： 一种方法包括在内壁上涂覆具有内壁和圆筒轴线的圆柱体，其中凝胶涂层涂覆在内壁上。 然后使包含溶剂的样品混合物在圆筒连续旋转的同时流过圆筒。 样品混合物最初被引导为沿着圆柱体轴线流动，使得来自样品混合物的样品颗粒从圆筒轴线朝向内壁加速，以形成嵌入凝胶涂层中的样品颗粒的膜。

公开(公告)号：US06856712B2

公开(公告)日：2005-02-15

申请号：US09994377

申请日：2001-11-26

申请人： Mark E. Fauver ,  Eric J. Seibel ,  Chris M. Brown ,  Per G. Reinhall ,  Quinn Y. J. Smithwick

发明人： Mark E. Fauver ,  Eric J. Seibel ,  Chris M. Brown ,  Per G. Reinhall ,  Quinn Y. J. Smithwick

IPC分类号： G02B6/26 ,  G02B6/32 ,  G02B6/35 ,  G02B26/10 ,  G02B6/00

CPC分类号： G02B6/241 ,  G02B6/245 ,  G02B6/25 ,  G02B6/262 ,  G02B6/32 ,  G02B6/3502 ,  G02B6/3586 ,  G02B26/10

摘要： Small, rugged scanners micro-fabricated from commercial optical fibers to form waveguides or other structures. The scanning waveguide has a distal portion on which is formed a non-linear taper with a diameter that decreases toward a distal end. Optionally, a hinge portion having a reduced diameter can be formed in the distal portion, improving the scanning properties of the waveguide. A micro-lens can be integrally formed at the distal tip of the waveguide with either a droplet of an optical adhesive, or by using an energy beam to melt the material of the waveguide to form a droplet. The droplet is shaped with an externally applied force. When mechanically driven in vibratory resonance, the tip of the optical waveguides moves in linear or two-dimensional scan patterns of relatively high amplitude and frequency, and large field of view. The scanner can be used either for image acquisition or image display.

摘要翻译： 小型，坚固的扫描仪，由商用光纤微型制造，形成波导或其他结构。 扫描波导具有远端部分，其上形成具有朝向远端减小的直径的非线性锥形。 可选地，可以在远端部分形成具有减小的直径的铰链部分，从而改善波导的扫描特性。 微透镜可以在波导的远端与光学粘合剂的液滴一体地形成，或者通过使用能量束来熔化波导的材料以形成液滴。 液滴以外部施加的力成形。 当以振动共振机械驱动时，光波导的尖端以相对高的振幅和频率以及较大的视场的线性或二维扫描图案移动。 扫描仪可用于图像采集或图像显示。