公开(公告)号：US20100021909A1

公开(公告)日：2010-01-28

申请号：US12502725

申请日：2009-07-14

Applicant: Michael Seul ,  Tatiana Vener ,  XiongWu Xia

Inventor： Michael Seul ,  Tatiana Vener ,  XiongWu Xia

IPC: C12Q1/68

CPC classification number: C12Q1/6876 ,  C12Q2600/16 ,  G06F19/20 ,  G06F19/22

Abstract: Disclosed is a method of iteratively optimizing two (or more) interrelated sets of probes for the multi-step analysis of sets of designated sequences, each such sequence requiring, for conversion, at least one conversion probe (“primer”), and each converted sequence requiring, for detection, at least one capture probe. The iterative method disclosed herein for the concurrent optimization of primer and probe selection invokes fast logical string matching functions to perform a complete cross-correlation of probe sequences and target sequences. The score function assigns to each probe-target alignment a “degree of matching” score on the basis of position-weighted Hamming distance functions introduced herein. Pairs of probes in the final selection may differ in several positions, while other pairs of probes may differ in only a single position. Not all such positions are of equal importance, and a score function is introduced, reflecting the position of the mismatch within the probe sequence.

Abstract translation: 公开了一种迭代优化两个（或多个）相互关联的探针组的方法，用于多步分析指定序列组，每个这样的序列需要用于转化的至少一个转化探针（“引物”），并且每个转化 用于检测至少一个捕获探针的序列。 本文公开的用于并行优化引物和探针选择的迭代方法调用快速逻辑串匹配函数来执行探针序列和靶序列的完全互相关。 得分函数根据本文介绍的位置加权汉明距离函数将每个探针 - 目标对准分配给“匹配度”得分。 最终选择中的一对探针在几个位置可能有所不同，而其他探针对只能在一个位置上不同。 并不是所有这样的位置都是同等重要的，并且引入了分数函数，反映了探针序列内不匹配的位置。

公开(公告)号：US20090313042A1

公开(公告)日：2009-12-17

申请号：US12546234

申请日：2009-08-24

Applicant: Yi Zhang ,  Michael Seul

Inventor： Yi Zhang ,  Michael Seul

IPC: G06Q50/00 ,  G06Q10/00

CPC classification number: G16H40/20 ,  G06Q30/02 ,  G06Q30/0202 ,  G06Q50/22 ,  G06Q50/24

Abstract: Disclosed is a registry for candidate transfusion donors, which invokes an inventory management policy to create and actively manage lists of candidate donors in order to minimize imbalances between demand and supply across multiple regions and across multiple categories of donors and recipients. Together with a genotyping laboratory, the registry does targeted recruitment of prospective donors who are typed for a set of genetic markers relating to clinically relevant antigens including mutations of Human Erythrocyte Antigens (HEA), genetic variants of Rh, and possibly additional antigens such as HLA and HPA. The registry monitors incoming demand for transfusion antigen genotypes, preferably stratify the demand into a set of categories representing stable subpopulations, and will apply strategies, disclosed herein, to tune the composition of candidate donor lists to match the demand, thereby avoiding excess, and unnecessary, typing of candidate donors.

Abstract translation: 公布了候选人输血捐助者的登记册，该登记处援引了库存管理政策，以创建和主动管理候选人捐助者名单，以便最大限度地减少多个地区和多个捐助者和接受者之间的需求与供应之间的不平衡。 与基因分型实验室一起，注册管理机构确实针对招募潜在的捐赠者，这些捐赠者是针对一系列与临床相关抗原相关的遗传标记，包括人类红细胞抗原（HEA）的突变，Rh的遗传变体和可能的其他抗原如HLA 和HPA。 注册管理机监测输血抗原基因型的输入需求，最好将需求分为一组代表稳定亚群体的类别，并将应用本文所披露的策略来调整候选捐献者名单的组成以匹配需求，从而避免过多和不必要的 ，候选人捐款人的打字。

公开(公告)号：US20060275799A1

公开(公告)日：2006-12-07

申请号：US11411510

申请日：2006-04-26

Applicant: Sukanta Banerjee ,  Jiacheng Yang ,  Michael Seul

Inventor： Sukanta Banerjee ,  Jiacheng Yang ,  Michael Seul

IPC: C40B40/08 ,  C40B30/06

CPC classification number: B01J19/0046 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00576 ,  B01J2219/00648 ,  B01J2219/00722 ,  B01J2219/00725 ,  C12N15/1093 ,  C40B20/04 ,  C40B50/14

Abstract: Disclosed are methods of for constructing a bead-displayed library of oligonucleotide probes (or sequence-modified capture moieties such as protein-nucleic acid conjugates) by ligation of a capture probe, having an analyte-specific sequence, to an anchor probe that is attached, at its 5′-end, (or possibly at the 3′ end) to an encoded carrier such as a color-coded microparticle (“bead”). Such a library can also be constructed by elongation of an anchor probe, using a second probe as the elongation template, wherein the second probe has an anchor-specific subsequence and an analyte-specific subsequence.

公开(公告)号：US20060240416A1

公开(公告)日：2006-10-26

申请号：US10032657

申请日：2001-12-28

Applicant: Sukanta Banerjee ,  Michael Seul

Inventor： Sukanta Banerjee ,  Michael Seul

IPC: C40B30/06 ,  C40B40/08

CPC classification number: G01N33/5434 ,  B01J19/0046 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00576 ,  B82Y15/00 ,  C12Q1/6837 ,  C12Q1/6876 ,  G01N27/745 ,  G01N33/54326 ,  G01N33/54333 ,  G01N33/54346 ,  G01N33/587 ,  G01N33/588

Abstract: The present invention provides a method for the generation of novel libraries of encoded magnetic particles from sub-libraries of by the generation of novel sub-libraries of magnetic nanoparticles and encoded particles. The sub-libraries are functionalized on demand are useful in the formation of arrays. The present invention is especially useful for performing multiplexed (parallel) assays for qualitative and/or quantitative analysis of binding interactions of a number of analyte molecules in a sample.

Abstract translation: 本发明提供了一种通过产生磁性纳米颗粒和编码颗粒的新型亚文库从子文库生成新的编码磁性颗粒文库的方法。 子库根据需要进行功能化在数组的形成中是有用的。 本发明特别可用于进行多个（并行）测定以定性和/或定量分析样品中许多分析物分子的结合相互作用。

公开(公告)号：US20060228744A1

公开(公告)日：2006-10-12

申请号：US11439697

申请日：2006-05-23

Applicant: Ghazala Hashmi ,  Michael Seul

Inventor： Ghazala Hashmi ,  Michael Seul

IPC: C12Q1/68 ,  C12P19/34

CPC classification number: C12Q1/6883 ,  C12Q1/6809 ,  C12Q1/6827 ,  C12Q1/6837 ,  C12Q2600/156 ,  C12Q2600/16 ,  Y10S436/80 ,  Y10S436/805 ,  Y10T436/143333 ,  C12Q2565/543 ,  C12Q2563/107 ,  C12Q2565/501

Abstract: Described are methods of assay design and assay image correction, useful for multiplexed genetic screening for mutations and polymorphisms, including CF-related mutants and polymorphs, using an array of probe pairs (in one aspect, where one member is complementary to a particular mutant or polymorphic allele and the other member is complementary to a corresponding wild type allele), with probes bound to encoded particles (e.g., beads) wherein the encoding allows identification of the attached probe. The methods relate to avoiding cross-hybridization by selection of probes and amplicons, as well as separation of reactions of certain probes and amplicons where a homology threshold is exceeded. Methods of correcting a fluorescent image using a background map, where the particles also contain an optical encoding system, are also disclosed.

公开(公告)号：US20060228740A1

公开(公告)日：2006-10-12

申请号：US11436717

申请日：2006-05-17

Applicant: Michael Seul

Inventor： Michael Seul

IPC: C12Q1/68 ,  C12M1/34

CPC classification number: C40B50/18 ,  B01J19/0046 ,  B01J2219/00432 ,  B01J2219/00448 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  C12Q1/6825 ,  C12Q1/6837 ,  C40B60/14 ,  C12Q2565/501 ,  C12Q2563/155 ,  C12Q2565/607

Abstract: A method and apparatus for the manipulation of colloidal particles and biomolecules at the interface between an insulating electrode such as silicon oxide and an electrolyte solution. Light-controlled electrokinetic assembly of particles near surfaces relies on the combination of three functional elements: the AC electric field-induced assembly of planar aggregates; the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The present invention provides a set of fundamental operations enabling interactive control over the creation and placement of planar arrays of several types of particles and biomolecules and the manipulation of array shape and size. The present invention enables sample preparation and handling for diagnostic assays and biochemical analysis in an array format, and the functional integration of these operations. In addition, the present invention provides a procedure for the creation of material surfaces with desired properties and for the fabrication of surface-mounted optical components.

公开(公告)号：US07083914B2

公开(公告)日：2006-08-01

申请号：US09448420

申请日：1999-11-22

Applicant: Michael Seul ,  Richard H. Ebright

Inventor： Michael Seul ,  Richard H. Ebright

IPC: C12Q1/68 ,  G01N33/53 ,  G01N33/543 ,  A61K38/00

CPC classification number: C40B20/04 ,  B01J19/0046 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00659 ,  B01J2219/00707 ,  B01J2219/00722 ,  B01J2219/00725 ,  C40B30/04 ,  C40B40/06 ,  C40B40/10 ,  C40B50/04 ,  C40B50/16 ,  C40B70/00 ,  G01N21/29 ,  G01N21/64 ,  G01N21/6458 ,  G01N33/54313 ,  G01N33/582 ,  G01N33/6845 ,  G01N2021/1765

Abstract: A method and apparatus for the physico-chemical encoding of a collection of beaded resin (“beads”) to determine the chemical identity of bead-anchored compounds by in-situ interrogation of individual beads. The present invention provides method and apparatus to implement color-coding strategies in applications and including the ultrahigh-throughput screening of bead-based combinatorial compounds libraries as well as multiplexed diagnostic and environmental testing and other biochemical assays.

Abstract translation: 用于物理化学编码串珠树脂（“珠粒”）的方法和装置，以通过单独珠粒的原位询问来确定珠状锚定化合物的化学特性。 本发明提供了在应用中实现颜色编码策略的方法和装置，并且包括基于珠的组合化合物文库的超高通量筛选以及多重诊断和环境测试以及其他生物化学测定。

公开(公告)号：US07057704B2

公开(公告)日：2006-06-06

申请号：US10098604

申请日：2002-03-16

Applicant: Michael Seul ,  Chiu Wo Chau

Inventor： Michael Seul ,  Chiu Wo Chau

IPC: G03B27/42 ,  G03B27/72

CPC classification number: G01N21/6456 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/089 ,  B01L2400/0415 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6463 ,  G01N2021/6478

Abstract: An apparatus providing programmable illumination pattern generation for the manipulation of colloidal particulates and biomolecules in suspension between electrodes, is disclosed. The apparatus implements LEAPS (Light-controlled electrokinetic assembly of particles near surfaces), which relies on: AC electric field-induced assembly of particles: the patterning of the electrolyte/silicon oxide/silicon interface to exert spatial control over the assembly process; and the real-time control of the assembly process via external illumination. The apparatus generates patterns of illumination and projects them on to planar surfaces. i.e., a LEAPS electrode. This enables the creation of patterns using graphical design or drawing software on a personal computer and the projection of said patterns, or sequences of patterns (“time-varying patterns”), onto the interface using a liquid crystal display (LCD) panel and an optical design which images the LCD panel onto the surface of interest, to provide for arrangements and assembly of particles in such patterns.

Abstract translation: 公开了一种提供用于在电极之间悬浮的胶体颗粒和生物分子的操作的可编程照明图案生成的装置。 该装置实现LEAPS（表面附近的颗粒的光控电动组装），其依赖于：AC电场引起的颗粒组装：电解质/氧化硅/硅界面的图案化以对组装过程施加空间控制; 并通过外部照明实现对装配过程的实时控制。 该设备产生照明模式并将它们投影到平面上。 即LEAPS电极。 这使得能够使用个人计算机上的图形设计或绘图软件来创建图案，并且使用液晶显示器（LCD）面板将所述图案或图案序列（“时变图案”）投影到界面上 将LCD面板成像到感兴趣的表面上的光学设计，以提供这种图案中的颗粒的布置和装配。

公开(公告)号：US20060068447A1

公开(公告)日：2006-03-30

申请号：US11253452

申请日：2005-10-19

Applicant: Sukanta Banerjee ,  Kairali Podual ,  Michael Seul

Inventor： Sukanta Banerjee ,  Kairali Podual ,  Michael Seul

IPC: G01N33/567 ,  G01N33/53 ,  B05D3/00

CPC classification number: C08J3/075 ,  C08J3/28 ,  C08J2333/26 ,  C08J2425/06 ,  C12Q1/6837 ,  G01N33/5005 ,  G01N33/545 ,  G03F7/0047 ,  G03F7/164 ,  Y10T428/249952 ,  Y10T428/25

Abstract: The present invention relates to a systematic process for the creation of functionally organized, spatially patterned assemblies polymer-microparticle composites including the AC electric field-mediated assembly of patterned, self supporting organic (polymeric) films and organic (polymeric)-microparticle composite films of tailored composition and morphology; the present invention further relates to the incorporation of said assemblies into other structures. The present invention. also relates to the application of such functional assemblies in materials science and biology. Additional areas of application include sensors, catalysts, membranes, micro-reactors, smart materials. Miniaturized format for generation of multifunctional thin films. Provides a simple set-up to synthesize thin films of tailored composition and morphology.

Abstract translation: 本发明涉及一种用于产生功能组织的空间图案化聚合物 - 聚合物 - 微粒复合材料的系统方法，其包括图案化的自支撑有机（聚合物）膜和有机（聚合） - 微粒复合膜的交流电场介导组装 定制组合和形态; 本发明还涉及将所述组件结合到其它结构中。 本发明。 还涉及这种功能组件在材料科学和生物学中的应用。 其他应用领域包括传感器，催化剂，膜，微反应器，智能材料。 用于生成多功能薄膜的小型化格式。 提供简单的设置来合成定制组合和形态的薄膜。

公开(公告)号：US20060025930A1

公开(公告)日：2006-02-02

申请号：US10909638

申请日：2004-08-02

Applicant: Xiongwu Xia ,  Michael Seul

Inventor： Xiongwu Xia ,  Michael Seul

IPC: G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC classification number: C12Q1/6827 ,  G06F19/18 ,  G06F19/22

Abstract: Disclosed are methods and algorithms (and their implementation) supporting the automated analysis and interactive review and refinement (“redaction”) of the analysis within an integrated software environment, for automated allele assignments. The implementation, preferably with a software system and a program referred to as the Automated Allele Assignment (“AAA”) program, provides a multiplicity of functionalities including: data management by way of an integrated interface to a portable database to permit visualizing, importing, exporting and creating customizable summary reports; system configuration (“Set-up”) including user authorization, training set analysis and probe masking; Pattern Analysis including string matching and probe flipping; and Interactive Redaction combining real-time database computations and “cut-and-paste” editing, generating “warning” statements and supporting annotation. It also includes a thresholding function, a method of setting thresholds, a method of refining thresholds by matching an experimental binary string (“reaction pattern”) setting for that probe, probe masking of signals produced by probes which do not contribute significantly to discriminating among alleles.

Abstract translation: 公开的方法和算法（及其实现）支持在集成软件环境中进行自动化等位基因分配的自动分析和交互式审查和细化（“编辑”）分析。 优选地使用软件系统和称为自动等位基因分配（“AAA”）程序的程序的实现提供了多种功能，包括：通过与便携式数据库的集成接口的数据管理，以允许可视化，导入， 出口和创建可定制的汇总报告; 系统配置（“设置”），包括用户授权，训练集分析和探测屏蔽; 模式分析包括字符串匹配和探针翻转; 和Interactive Redaction将实时数据库计算和“剪切和粘贴”编辑相结合，生成“警告”语句和支持注释。 它还包括阈值功能，设置阈值的方法，通过匹配用于该探针的实验二进制串（“反应模式”）设置来精确化阈值的方法，探针对由探针产生的信号进行探针掩蔽，所述探针不对显着区别于 等位基因