Fructosylamine deglycase and a method of producing it

    公开(公告)号:US5387109A

    公开(公告)日:1995-02-07

    申请号:US66499

    申请日:1993-05-24

    CPC分类号: C12N9/0012 C12Q1/26

    摘要: Disclosed are a novel fructosylamine deglycase characterized by the specificity to amadori compounds of catalyzing oxidation of the compounds to produce an .alpha.-ketoaldehyde, an amine derivative and hydrogen peroxide; a method of producing the novel enzyme by cultivating microorganisms belonging to the genus Candida and having an ability of producing the novel enzyme; and a method of quantitative determination of amadori compounds by applying the novel enzyme to a sample containing amadori compounds to measure the amount of the hydrogen peroxide to be formed by the oxidation reaction or measure the amount of the oxygen to be consumed by the reaction to thereby obtain the amount of the amadori compounds from the measured value. The invention provides a novel enzyme characterized by the high specificity to the reaction with amadori compounds, especially that having therein a saccharide moiety as bonded to the .epsilon.-amino group, and also provides a method of producing the enzyme and a method of quantitative determination of amadori compounds with the enzyme. Using the enzyme, quantitative determination of amadori compounds, which is difficult by conventional enzymatic methods, is possible with ease. In particular, in measurement of the amount of a saccharified protein in a sample from a living body, which is an important index substance in diagnosis of diabetes, the enzymatic method of the invention of measuring fructosamine in the sample is hardly influenced by other interfering substances or impurities in the sample, the influence by them having been inevitable in conventional chemical methods. Accurate determination of the amount is possible by the method, and the method is free from the inconvenience of staining the kits and instruments used. For measuring glycohemoglobin, the method displays another characteristic feature that its operation is more simple and needs less labor and time than conventional methods.

    Methods for protecting vegetables, turfgrass, rice and fruit trees from
fungi and bacteria
    16.
    发明授权
    Methods for protecting vegetables, turfgrass, rice and fruit trees from fungi and bacteria 失效
    从真菌和细菌中保护蔬菜,草坪草,水稻和果树的方法

    公开(公告)号:US5374627A

    公开(公告)日:1994-12-20

    申请号:US006145

    申请日:1993-01-19

    CPC分类号: A01N43/16 A01N63/00 A01N65/20

    摘要: A variety of plant diseases and damage by certain pests in agricultural and horticultural plants can be efficiently controlled without substantial chemical injuries by applying a plant-protecting composition which comprises (a) 1 part by weight of a chitosan hydrolyzate having an average molecular weight of 10,000 to 50,000, obtained by acid hydrolysis or enzymatic hydrolysis of chitosan and (b) 0.25 to 4 parts by weight of acetic acid. Any possible chemical injury to plants caused by applying the composition can be further reduced when the composition is admixed with a deproteinized juice of alfalfa leaves.

    摘要翻译: 通过施用植物保护组合物可以有效地控制农业和园艺植物中的某些害虫的各种植物病害和损害,而不会造成实质的化学损伤,所述植物保护组合物包含(a)1重量份的平均分子量为10,000的壳聚糖水解产物 至50,000,通过壳聚糖的酸水解或酶水解获得,和(b)0.25至4重量份的乙酸。 当将组合物与脱蛋白的苜蓿叶汁混合时,通过施用组合物引起的对植物的任何可能的化学损伤可以进一步降低。

    Process for producing starch noodles by extrusion
    17.
    发明授权
    Process for producing starch noodles by extrusion 失效
    通过挤压生产淀粉面的方法

    公开(公告)号:US5916616A

    公开(公告)日:1999-06-29

    申请号:US917531

    申请日:1997-08-26

    摘要: A process for producing starch noodles, which comprises adding 45-55 parts by weight of hot water to 100 parts by weight of starch obtained from at least one member-selected from the group consisting of potato, sweet potato, tapioca, corn, wheat and a product thereof followed by being mixed to prepare large particles of dough and then extruding the dough under degassing at degrees of vacuum of not less than 650 Torr to produce a dough sheet. Starch noodles can be produced efficiently in simple procedures without separately preparing starch paste and without using special rollers. Further, starch noodles thus produced are highly transparent and less melted by boiling.

    摘要翻译: 一种生产淀粉面的方法,其包括将45-55重量份的热水加入至100重量份从至少一种选自马铃薯,甘薯,木薯,玉米,小麦和 然后将其产物混合以制备面团的大颗粒,然后在不低于650乇的真空度下将生面团在脱气下挤出以制备面片。 淀粉面可以在简单的程序中有效地生产,而无需分开制备淀粉糊,而不使用特殊的辊。 此外,由此生产的淀粉面条是高度透明的,并且通过沸腾较少熔化。