公开(公告)号：US09068213B2

公开(公告)日：2015-06-30

申请号：US13941346

申请日：2013-07-12

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: C12N1/12 ,  C12P33/00 ,  C12P7/64 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  A23D9/00 ,  C07C57/03 ,  C07J9/00 ,  C12N15/82 ,  C12R1/89

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

Abstract translation: 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP脱氧酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US08945908B2

公开(公告)日：2015-02-03

申请号：US13865974

申请日：2013-04-18

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: C12N1/12 ,  C12P7/64 ,  C12P33/00 ,  A23D9/00 ,  C07C57/03 ,  C07J9/00

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

Abstract translation: 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP脱氧酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US20140315267A1

公开(公告)日：2014-10-23

申请号：US14276943

申请日：2014-05-13

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

IPC: C12P7/64

CPC classification number: C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

Abstract: Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

Abstract translation: 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造运输燃料如可再生柴油，生物柴油和可再生喷气燃料，以及油脂化学品 例如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：US08846375B2

公开(公告)日：2014-09-30

申请号：US13941353

申请日：2013-07-12

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  George Rudenko ,  Riyaz Bhat ,  Xinhua Zhao ,  Risha Bond ,  Walter Rakitsky ,  Alejandro Marangoni ,  Diza Braksmayer

IPC: A23D9/00 ,  C12P7/64 ,  C12P33/00 ,  C07J9/00 ,  C07C57/03

CPC classification number: C12P7/6409 ,  A23D9/00 ,  A23D9/013 ,  C07C57/03 ,  C07J9/00 ,  C11B1/00 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N9/2402 ,  C12N15/113 ,  C12N15/8247 ,  C12N2310/531 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/6472 ,  C12P33/00 ,  C12R1/89 ,  C12Y203/01015 ,  C12Y203/0102 ,  C12Y203/01041 ,  C12Y203/01051 ,  C12Y203/01179 ,  C12Y203/0118 ,  C12Y302/01014 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P20/52

Abstract: Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type.

Abstract translation: 重组DNA技术用于产生产生具有所需脂肪酸谱和区域特异性或立体特异性谱的甘油三酯油的含油重组细胞。 基因操作包括编码硬脂酰-ACP脱氧酶，δ12脂肪酸去饱和酶，酰基-ACP硫酯酶，酮酰基-ACP合成酶和溶血磷脂酰基转移酶的那些。 所生产的油可以具有增强的氧化或热稳定性，或可用作煎炸油，起酥油，卷入起酥油，回火脂肪，可可脂替代物，润滑剂或用作各种化学方法的原料。 脂肪酸分布可以富集中链型，或者油可富含饱和不饱和饱和型甘油三酸酯。

公开(公告)号：US08822176B2

公开(公告)日：2014-09-02

申请号：US13889214

申请日：2013-05-07

Applicant: Solazyme, Inc.

Inventor： Anthony G. Day ,  Geoffrey Brooks ,  Scott Franklin

IPC: C12P1/00

CPC classification number: C11B3/02 ,  A23D7/00 ,  A23D9/00 ,  C11D9/26 ,  C12P7/6418 ,  C12P7/6463 ,  C12P7/649 ,  Y02E50/13

Abstract: Provided are methods for preparing chemically modified lipids. The lipids are obtained from heterotrophically cultured microalgae and are subjected to a transesterification or interesterification reaction. The microalgae include those from the genus Parachlorella, Prototheca, Chlorella, or strains having at least 85% nucleotide sequence identity in 23S rRNA sequences to a Parachlorella, Prototheca, or Chlorella strain that are cultured in a bioreactor substantially in the absence of light.

Abstract translation: 提供制备化学修饰的脂质的方法。 脂质从异养培养的微藻获得并进行酯交换或酯交换反应。 微藻包括来自Parachlorella属，Prototheca属，小球藻属或在23S rRNA序列中与在不存在光的生物反应器中培养的Parachlorella，Prototheca或Chlorella菌株具有至少85％核苷酸序列同一性的微藻。

公开(公告)号：US20140178950A1

公开(公告)日：2014-06-26

申请号：US14099704

申请日：2013-12-06

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Shane Brubaker ,  George N. Rudenko ,  Riyaz Bhat ,  Matthew Shoa-Azar ,  Aravind Somanchi ,  Aren Ewing

IPC: C12P7/64

CPC classification number: C12P7/6463 ,  C12N15/52

Abstract: Genetically engineered microbial, e.g., Chlorella protothecoides, cells producing microbial oils are useful as a food additive and a source of renewable fuels and industrial chemicals. Lipid biosynthesis genes for Auxenochlorella protethecoides are disclosed that are useful for increasing lipid production and altering fatty acid and triacylglycerol profiles in recombinant microrganisms including cells of the genus Auxenochlorella or Prototheca.

Abstract translation: 基因工程微生物，例如原型小球藻，产生微生物油的细胞可用作食品添加剂和可再生燃料和工业化学品的来源。 本发明公开了用于增加油类生物合成的脂质体生物合成基因，其可用于增加脂质生产和改变重组微生物（包括欧洲绿叶藻或原生动物）的重组微生物中的脂肪酸和三酰甘油轮廓。

公开(公告)号：US20140170716A1

公开(公告)日：2014-06-19

申请号：US14184288

申请日：2014-02-19

Applicant: Solazyme, Inc.

Inventor： Donald E. Trimbur ,  Chung-Soon Im ,  Harrison F. Dillon ,  Anthony G. Day ,  Scott Franklin ,  Anna Coragliotti

IPC: C12P7/64

CPC classification number: C10L1/026 ,  C07H21/04 ,  C10L1/06 ,  C10L2200/0484 ,  C10L2270/026 ,  C10L2270/04 ,  C11C3/00 ,  C12N1/12 ,  C12N1/32 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1205 ,  C12N9/14 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2408 ,  C12N9/2431 ,  C12N9/88 ,  C12N15/79 ,  C12P5/02 ,  C12P7/6409 ,  C12P7/6436 ,  C12P7/6445 ,  C12P7/6463 ,  C12P7/649 ,  C12P21/06 ,  C12Y302/01026 ,  Y02E50/10 ,  Y02E50/13 ,  Y02E50/343 ,  Y02P20/52 ,  Y02T50/678

Abstract: The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel.

Abstract translation: 本发明提供了通过含油微生物发酵产生的甘油三酯油制备烷烃的方法。 本文提供的方法可以利用多种碳水化合物原料，包括甘蔗渣，甜菜浆，玉米秸秆，甘油，玉米淀粉，高粱，糖蜜，废甘油和其他可再生材料。 这些方法还包括与含油微生物一起使用的加氢处理，加氢裂化，异构化，蒸馏和其它石油化学方法以及由其衍生的产物来制造燃料。 具体实施方案包括制造ASTM D975和符合ASTM D1655的燃料。 本文提供的基因工程微生物可用于制造可再生柴油和可再生喷气燃料。

公开(公告)号：US09388435B2

公开(公告)日：2016-07-12

申请号：US14730671

申请日：2015-06-04

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Walter Rakitsky ,  George Rudenko ,  Xinhua Zhao ,  Felipe Arana Rodriguez ,  Wenhua Lu ,  Janice Wee

IPC: C11B1/00 ,  C12P7/64 ,  C10M169/04 ,  C11B3/00 ,  A23D9/02 ,  A23D9/007 ,  C11B1/02 ,  C11B1/06 ,  C11B1/10 ,  C11B3/06 ,  C11B3/10 ,  C11B3/14 ,  H01B3/20 ,  H01F27/10

CPC classification number: C12P7/6463 ,  A23D9/007 ,  A23D9/02 ,  C10M169/041 ,  C11B1/025 ,  C11B1/06 ,  C11B1/10 ,  C11B3/001 ,  C11B3/006 ,  C11B3/008 ,  C11B3/06 ,  C11B3/10 ,  C11B3/14 ,  C12N9/2442 ,  C12N15/79 ,  C12P7/6409 ,  C12Y301/02014 ,  H01B3/20 ,  H01F27/105 ,  H01F27/321

Abstract: Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids.

公开(公告)号：US09290749B2

公开(公告)日：2016-03-22

申请号：US13837996

申请日：2013-03-15

Applicant: Solazyme, Inc.

Inventor： George N. Rudenko ,  Jason Casolari ,  Scott Franklin

IPC: C12N15/82 ,  C12N5/14 ,  C12N9/18 ,  C07H21/04 ,  C12N9/16 ,  C12P7/64

CPC classification number: C12P7/6409 ,  A23L33/115 ,  A23V2002/00 ,  C10L1/02 ,  C10L2200/0476 ,  C10L2290/26 ,  C12N9/16 ,  C12P7/6463 ,  C12Y301/02014

Abstract: The invention features plant acyl-ACP thioesterase genes of the FatB class and proteins encoded by these genes. The genes are useful for constructing recombinant host cells having altered fatty acid profiles. Oleaginous microalga host cells with the new genes or previously identified FatB genes are disclosed. The microalgae cells produce triglycerides with useful fatty acid profiles.

Abstract translation: 本发明具有FatB类的植物酰基-ACP硫酯酶基因和由这些基因编码的蛋白质。 该基因可用于构建具有改变的脂肪酸谱的重组宿主细胞。 公开了具有新基因或先前鉴定的FatB基因的嗜酸微藻宿主细胞。 微藻细胞产生具有有用脂肪酸分布的甘油三酯。

公开(公告)号：US20160024538A1

公开(公告)日：2016-01-28

申请号：US14819117

申请日：2015-08-05

Applicant: Solazyme, Inc.

Inventor： Scott Franklin ,  Aravind Somanchi ,  Janice Wee ,  George Rudenko ,  Jeffrey L. Moseley ,  Walt Rakitsky

IPC: C12P7/64 ,  C11C3/00

CPC classification number: C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

Abstract: Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

Abstract translation: 提供了用于在重组微生物中生产食品组合物，油，燃料，油脂化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造食品组合物，以及运输燃料如可再生柴油，生物柴油和可再生喷气燃料， 以及诸如功能流体，表面活性剂，肥皂和润滑剂的油脂化学品。