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    Method of making biochips and the biochips resulting therefrom
    16.
    发明授权
    Method of making biochips and the biochips resulting therefrom 失效
    制作生物芯片的方法及由此产生的生物芯片

    公开(公告)号：US06174683B1

    公开(公告)日：2001-01-16

    申请号：US09299831

    申请日：1999-04-26

    申请人： Soonkap Hahn ,  Roberto Fagnani

    发明人： Soonkap Hahn ,  Roberto Fagnani

    IPC分类号： C12Q168

    CPC分类号： G01N33/54353 ,  G01N33/545

    摘要： Methods for preparing a biochip are provided herein wherein the biomolecular probe to be used with the biochip is alternatively bound to a hydrogel prepolymer prior to or simultaneously with polymerization of the prepolymer. In particularly preferred embodiments, a polyurethane-based hydrogel prepolymer is derivatized with an organic solvent soluble biomolecule, such as a peptide nucleic acid probe in aprotic, organic solvent. Following derivatization of the prepolymer, an aqueous solution, for example sodium bicarbonate, preferably buffered to a pH of about 7.2 to about 9.5, is added to the derivatized prepolymer solution to initiate polymerization of the hydrogel. Alternatively, a water soluble biomolecule, such as DNA or other oligonucleotide, is prepared in an aqueous solution and added to the polyurethane-based hydrogel prepolymer such that derivatization and polymerization occur, essentially, simultaneously. While the hydrogel is polymerizing, it is microspotted onto a solid substrate, preferably a silanated glass substrate, to which the hydrogel microdroplet becomes covalently bound. Most preferably the hydrogel microdroplets are at least about 30 &mgr;m thick, for example about 50 &mgr;m to about 100 &mgr;m thick. The resulting biochips are particularly useful for gene discovery, gene characterization, functional gene analysis and related studies.

    摘要翻译： 本文提供了制备生物芯片的方法，其中与生物芯片一起使用的生物分子探针在预聚物聚合之前或同时与水凝胶预聚物交替结合。 在特别优选的实施方案中，聚氨酯基水凝胶预聚物用有机溶剂可溶性生物分子衍生，例如非质子有机溶剂中的肽核酸探针。 在预聚物衍生化之后，向衍生化的预聚物溶液中加入优选缓冲至pH约7.2至约9.5的碳酸氢钠水溶液以引发水凝胶的聚合。 或者，在水溶液中制备水溶性生物分子，例如DNA或其它寡核苷酸，并加入到基于聚氨酯的水凝胶预聚物中，使得衍生化和聚合基本上同时发生。 当水凝胶聚合时，将其微滴在固体基质上，优选硅烷化的玻璃底物，水凝胶微滴变成共价键。 最优选地，水凝胶微滴至少约30μm厚，例如约50μm至约100μm厚。 所得到的生物芯片对于基因发现，基因表征，功能基因分析和相关研究特别有用。

    Child-birth assisting system
    17.
    发明授权
    Child-birth assisting system 失效
    育儿辅助系统

    公开(公告)号：US5405356A

    公开(公告)日：1995-04-11

    申请号：US86099

    申请日：1993-06-30

    申请人： Soonkap Hahn ,  Wei Pan ,  Ron Yamamoto ,  Peter M. Lloyd ,  Mingying Gai

    发明人： Soonkap Hahn ,  Wei Pan ,  Ron Yamamoto ,  Peter M. Lloyd ,  Mingying Gai

    IPC分类号： A61B5/00 ,  A61B17/135 ,  A61B17/42

    CPC分类号： A61B17/42 ,  A61B17/1355

    摘要： The childbirth-assisting device uses an automatically synchronized expandable pneumatic girdle-to externally augment the secondary force of labor. The pneumatic girdle is fitted around the abdomen of the woman and the girdle is inflated to create a downward pressure on the abdomen when a contraction occurs. The synchronization of the girdle's inflation and the contractions is provided by an electronic controller which receives a signal from an intra-uterine monitor indicating a contraction and causes the girdle to inflate at a certain rate until a preset intra-uterine pressure is attained. Once the intra-uterine pressure reaches the pre-set pressure, the girdle pressure is maintained until the offset of the contraction is detected, at which time the girdle is deflated.

    摘要翻译： 分娩辅助装置使用自动同步的可膨胀气动腰带 - 以外部增强第二劳动力。 气动腰带安装在女人的腹部周围，腰带充气，当收缩发生时，腹部产生向下的压力。 腰带充气和收缩的同步由电子控制器提供，该电子控制器接收来自子宫内监视器的指示收缩的信号，并使腰带以一定的速率膨胀，直到达到预设的子宫内压力。 一旦子宫内压力达到预设压力，则保持腰带压力，直到检测到收缩的偏移，此时腰围放气。

    Method of stabilizing a carbon dioxide sensor
    20.
    发明授权
    Method of stabilizing a carbon dioxide sensor 失效
    稳定二氧化碳传感器的方法

    公开(公告)号：US5246859A

    公开(公告)日：1993-09-21

    申请号：US888550

    申请日：1992-05-22

    申请人： Alan Nelson ,  Henry K. Hui ,  Monte Bennett ,  Soonkap Hahn ,  Charles S. Bankert ,  Jeffrey T. Jackson

    发明人： Alan Nelson ,  Henry K. Hui ,  Monte Bennett ,  Soonkap Hahn ,  Charles S. Bankert ,  Jeffrey T. Jackson

    IPC分类号： A61B5/145 ,  A61B5/1459 ,  G01N31/22 ,  G01N33/49 ,  G01N33/84

    CPC分类号： G01N33/84 ,  G01N31/223 ,  Y10T436/102499 ,  Y10T436/108331 ,  Y10T436/20 ,  Y10T436/204998 ,  Y10T436/255

    摘要： The method of stabilizing a carbon dioxide sensor involves formulation of a bicarbonate buffer solution in the sensor with a bicarbonate ion concentration of from about 100 mM to about 200 mM bicarbonate. The method also involves treatment of the sensor to reduce the instability that may occur in carbon dioxide sensors when such sensors are exposed to either very low or very high carbon dioxide levels for extended periods of time. The sensor is treated by exposing the sensor to an aqueous solution containing at least 2 weight percent carbon dioxide, for from several days to several months. The solution may be prepared in advance, or may be dynamically infused with carbon dioxide to provide the desired carbon dioxide content.

    摘要翻译： 稳定二氧化碳传感器的方法包括在传感器中配制碳酸氢盐缓冲溶液，碳酸氢根离子浓度为约100mM至约200mM碳酸氢盐。 该方法还涉及传感器的处理，以减少二氧化碳传感器当这种传感器长时间暴露于非常低或非常高的二氧化碳水平时可能发生的不稳定性。 通过将传感器暴露于含有至少2重量％二氧化碳的水溶液中来处理传感器，持续数天至数月。 该溶液可以预先制备，或者可以动态地注入二氧化碳以提供所需的二氧化碳含量。