公开(公告)号：US20180327756A1

公开(公告)日：2018-11-15

申请号：US15230161

申请日：2016-08-05

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

发明人： Feng Zhang ,  Le Cong ,  David Benjamin Turitz Cox ,  Patrick Hsu ,  Shuailiang Lin ,  Fei Ran ,  Randall Jeffrey Platt ,  Neville Espi Sanjana

IPC分类号： C12N15/74 ,  C12N9/22 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/85 ,  C12N15/90 ,  C12N15/10

CPC分类号： C12N15/746 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/907 ,  C12N2310/20 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2800/101

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

公开(公告)号：US20180312875A1

公开(公告)日：2018-11-01

申请号：US16033005

申请日：2018-07-11

申请人： EMMANUELLE CHARPENTIER ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  UNIVERSITY OF VIENNA

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  Emmanuelle Charpentier

IPC分类号： C12N15/90 ,  A61K38/46 ,  C12N9/22 ,  C12N15/10 ,  C12N15/11 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  A61K48/00

CPC分类号： C12N15/907 ,  A01H6/4684 ,  A01K67/027 ,  A61K38/465 ,  A61K48/00 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/746 ,  C12N15/90 ,  C12N15/902 ,  C12N2310/11 ,  C12N2310/13 ,  C12N2310/14 ,  C12N2310/20 ,  C12N2310/31 ,  C12N2310/32 ,  C12N2310/33 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2800/80 ,  C12Y301/04

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US20180236013A1

公开(公告)日：2018-08-23

申请号：US15894408

申请日：2018-02-12

申请人： REGENTS OF THE UNIVERSITY OF MINNESOTA

发明人： Yiannis J. Kaznessis ,  Katherine G. Volzing ,  Juan Borrero Del Pino ,  Gary Dunny

IPC分类号： A61K35/744 ,  A01N63/00 ,  C12N15/74 ,  C07K7/08 ,  A61K38/16 ,  A61K38/10 ,  C07K14/00 ,  A61K35/00

CPC分类号： A61K35/744 ,  A01N63/00 ,  A61K38/10 ,  A61K38/16 ,  A61K2035/11 ,  C07K7/08 ,  C07K14/001 ,  C12N15/746

摘要： Provided herein are genetically modified microbes. In one embodiment, a genetically modified microbe includes an exogenous polynucleotide that includes a pheromone-responsive region. In one embodiment, the pheromone-responsive region is derived from a conjugative plasmid from a member of the genus Enterococcus spp. The pheromone-responsive region includes a pheromone-responsive promoter and an operably linked coding region encoding an antimicrobial peptide. In one embodiment, a genetically modified microbe includes an exogenous polynucleotide that includes a promoter and an operably linked coding sequence encoding an antimicrobial peptide, where expression of the coding region is controlled by a modulator polypeptide and is altered by a modulating agent, and where the coding region encodes an antimicrobial peptide. Also provided herein are methods of using the genetically modified microbes, including methods for inhibiting growth of an Enterococcus spp., a pathogenic E. coli, or a pathogenic Salmonella spp., for treating a subject, and for modifying a subject's gastrointestinal microflora.

公开(公告)号：US10017751B2

公开(公告)日：2018-07-10

申请号：US15532566

申请日：2015-12-04

申请人： GODO SHUSEI CO., LTD.

发明人： Hirofumi Horiguchi ,  Ai Iyotani ,  Kazuma Shiota

IPC分类号： C12P19/22 ,  C12N9/26 ,  C12N15/70 ,  C12N15/74 ,  C12N15/81 ,  C12P19/16 ,  C13K7/00

CPC分类号： C12N9/2411 ,  C12N5/10 ,  C12N15/09 ,  C12N15/70 ,  C12N15/746 ,  C12N15/81 ,  C12P19/12 ,  C12P19/16 ,  C12P19/22 ,  C12Y302/01068 ,  C13K7/00

摘要： An isoamylase having improved heat resistance and an industrial method for producing maltose from starch.The isoamylase is an isoamylase consisting of the amino acid sequence represented by SEQ ID NO: 1 or an isoamylase resulting from deletion, substitution, or insertion of one to several amino acid residues in the amino acid sequence represented by SEQ ID NO: 1, wherein at least valine at amino acid number 515 and methionine at amino acid number 570 are mutated to other amino acids.

公开(公告)号：US09951342B2

公开(公告)日：2018-04-24

申请号：US14570994

申请日：2014-12-15

申请人： DUPONT NUTRITION BIOSCIENCES APS

发明人： Rodolphe Barrangou ,  Christophe Fremaux ,  Phillippe Horvath ,  Dennis Romero ,  Patrick Boyaval

IPC分类号： C12Q1/68 ,  C12N15/74 ,  A23C9/123 ,  C12N1/20 ,  C12Q1/70

CPC分类号： C12N15/746 ,  A23C9/123 ,  A23C9/1238 ,  A23C2220/202 ,  A23Y2240/75 ,  C12N1/20 ,  C12N2795/00011 ,  C12Q1/70 ,  C12Q2525/151

摘要： The present invention provides methods and compositions related to modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some preferred embodiments, the present invention provides compositions and methods for the use of one or more cas genes or proteins for modulating the resistance of a cell against a target nucleic acid or a transcription product thereof. In some embodiments, the present invention provides methods and compositions that find use in the development and use of strain combinations and starter culture rotations. In additional embodiments, the present invention provides methods for labelling and/or identifying bacteria. In some preferred embodiments, the present invention provides methods for the use of CRISPR loci to determine the potential virulence of a phage against a cell and the use of CRISPR-cas to modulate the genetic sequence of a phage for increased virulence level. In still further embodiments, the present invention provides means and compositions for the development and use of phages as biocontrol agents.

公开(公告)号：US09862976B2

公开(公告)日：2018-01-09

申请号：US15067421

申请日：2016-03-11

申请人： BUTAMAX ADVANCED BIOFUELS LLC

发明人： Gail K. Donaldson ,  Andrew C. Eliot ,  Dennis Flint ,  Lori Ann Maggio-Hall ,  Vasantha Nagarajan

IPC分类号： C12N15/52 ,  C12N9/02 ,  C12N9/04 ,  C12N9/88 ,  C12P7/16 ,  C12N9/10 ,  C12N9/06 ,  C12N15/81 ,  C12N15/70 ,  C12N15/75 ,  C12N15/74

CPC分类号： C12P7/16 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/0016 ,  C12N9/1022 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12N15/70 ,  C12N15/74 ,  C12N15/746 ,  C12N15/75 ,  C12N15/81 ,  C12Y101/01001 ,  C12Y101/01002 ,  C12Y101/01086 ,  C12Y102/00 ,  C12Y102/01003 ,  C12Y102/04004 ,  C12Y202/01006 ,  C12Y401/01072 ,  C12Y402/01009 ,  Y02E50/10 ,  Y02E50/343 ,  Y02P20/52

摘要： Methods for the fermentative production of four carbon alcohols is provided. Specifically, butanol, preferably isobutanol is produced by the fermentative growth of a recombinant bacterium expressing an isobutanol biosynthetic pathway.

公开(公告)号：US09822372B2

公开(公告)日：2017-11-21

申请号：US14990444

申请日：2016-01-07

申请人： THE BROAD INSTITUTE INC. ,  MASSACHUSETTS INSTITUTE OF TECHNOLOGY ,  THE ROCKEFELLER UNIVERSITY

发明人： Feng Zhang ,  David Benjamin Turitz Cox ,  Luciano Marraffini ,  David Olivier Bikard ,  Wenyan Jiang ,  Neville Espi Sanjana

IPC分类号： C12Q1/02 ,  C12N15/74 ,  C12N9/22 ,  C12N15/63 ,  C12N15/85 ,  C12N15/70 ,  C12N15/90 ,  C12N15/113 ,  C12N1/20 ,  C12N15/00 ,  C12P21/04 ,  C12Q1/68 ,  C12N15/10

CPC分类号： C12N15/746 ,  C12N9/22 ,  C12N15/102 ,  C12N15/1082 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/907 ,  C12N2310/20 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2800/101

摘要： The invention provides for systems, methods, and compositions for manipulation of sequences and/or activities of target sequences. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for selecting specific cells by introducing precise mutations utilizing the CRISPR/Cas system.

公开(公告)号：US09790510B2

公开(公告)日：2017-10-17

申请号：US14934572

申请日：2015-11-06

申请人： INTREXON ACTOBIOTICS NV

发明人： Erik Remaut ,  Dirk Iserentant

IPC分类号： C12N1/04 ,  C12N15/74

CPC分类号： C12N15/746 ,  C12N1/04

摘要： Lactococcus lactis strains with improved preservation characteristics, and improved acid and bile salt tolerance are disclosed. More specifically, a L. lactis strain having a heterologous trehalose-6-phosphate synthase gene and/or a trehalose-6-phosphate phosphatase gene, results in an accumulation of trehalose in the cytoplasm and/or in the cytoplasmic membrane. A L. lactis strain, having an internal trehalose concentration of at least 10 mg per gram cells (w/w) is disclosed.

公开(公告)号：US20170051310A1

公开(公告)日：2017-02-23

申请号：US15138604

申请日：2016-04-26

申请人： Emmanuelle Charpentier ,  The Regents of the University of California ,  University of Vienna

发明人： Jennifer A. Doudna ,  Martin Jinek ,  Krzysztof Chylinski ,  James Harrison Doudna Cate ,  Wendell Lim ,  Lei Qi ,  Emmanuelle Charpentier

IPC分类号： C12N15/90

CPC分类号： C12N15/907 ,  A01H6/4684 ,  A01K67/027 ,  A61K38/465 ,  A61K48/00 ,  C12N9/22 ,  C12N15/102 ,  C12N15/111 ,  C12N15/113 ,  C12N15/63 ,  C12N15/70 ,  C12N15/746 ,  C12N15/90 ,  C12N15/902 ,  C12N2310/11 ,  C12N2310/13 ,  C12N2310/14 ,  C12N2310/20 ,  C12N2310/31 ,  C12N2310/32 ,  C12N2310/33 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2800/80 ,  C12Y301/04

摘要： The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms.

公开(公告)号：US09549956B2

公开(公告)日：2017-01-24

申请号：US14263306

申请日：2014-04-28

申请人： KIRIN HOLDINGS KABUSHIKI KAISHA

发明人： Daisuke Fujiwara ,  Kenta Jonai ,  Tetsu Sugimura

IPC分类号： A61K35/74 ,  A61K35/744 ,  A23C9/123 ,  A61K35/747 ,  C12N1/20 ,  A23C19/06 ,  A61K31/711 ,  C12N15/74 ,  C12Q1/68 ,  G01N33/68 ,  A23C19/032

CPC分类号： A61K35/744 ,  A23C9/123 ,  A23C9/1236 ,  A23C19/0323 ,  A23C19/062 ,  A23L33/135 ,  A23Y2240/41 ,  A61K9/0053 ,  A61K31/711 ,  A61K35/747 ,  C12N1/20 ,  C12N15/746 ,  C12Q1/6809 ,  C12R1/01 ,  G01N33/6866

摘要： This invention provides an IFN inducer comprising, as an active ingredient, lactic acid bacteria and capable of inducing IFN production, an immunopotentiating agent or prophylactic agent against virus infection comprising such inducer, and a food or drink product comprising such IFN inducer and having IFN-inducing activity, immunopotentiating activity, or prophylactic activity against virus infection. The agent for inducing IFN production comprises, as active ingredients, lactic acid bacteria that can activate plasmacytoid dendritic cells (pDCs) and promote IFN production, such as Lactococcus garvieae NBRC100934, Lactococcus lactis subsp. cremoris JCM16167, Lactococcus lactis subsp. cremoris NBRC100676, Lactococcus lactis subsp. hordniae JCM1180, Lactococcus lactis subsp. hordniae JCM11040, Lactococcus lactis subsp. lactis NBRC12007, Lactococcus lactis subsp. lactis NRIC1150, Lactococcus lactis subsp. lactis JCM5805, Lactococcus lactis subsp. lactis JCM20101, Leuconostoc lactis NBRC12455, Leuconostoc lactis NRIC1540, Pediococcus damnosus JCM5886, or Streptococcus thermophilus TA-45.

摘要翻译： 本发明提供一种IFN诱导剂，其包含作为活性成分的乳酸菌并能够诱导IFN产生，包含该诱导剂的免疫增强剂或抗病毒感染的预防剂，以及包含该IFN诱导剂的食品或饮品， 诱导活性，免疫增强活性或针对病毒感染的预防活性。 用于诱导IFN产生的药物包括作为活性成分的可激活浆细胞样树突状细胞（pDC）并促进IFN产生的乳酸菌，例如乳球乳杆菌NBRC100934，乳酸乳球菌亚种。 cremoris JCM16167，乳酸乳球菌亚种 cremoris NBRC100676，乳酸乳球菌亚种 大麦草JCM1180，乳酸乳球菌亚种 大麦草JCM11040，乳酸乳球菌亚种 乳酸菌NBRC12007，乳酸乳球菌亚种 乳酸菌NRIC1150，乳酸乳球菌亚种 乳酸菌JCM5805，乳酸乳球菌亚种 乳酸菌JCM20101，乳酸列表克鲁维酵母NBRC12455，乳酸列表氨酸亮氨酸NRIC1540，侏儒球菌JCM5886或嗜热链球菌TA-45。