公开(公告)号：US20240151628A1

公开(公告)日：2024-05-09

申请号：US18414008

申请日：2024-01-16

申请人： CYTONOME/ST, LLC

发明人： Johnathan Charles Sharpe ,  Kiryakos S. Mutafopulos

IPC分类号： G01N15/10

CPC分类号： G01N15/10 ,  G01N2015/1006 ,  G01N2015/1028

摘要： A method, system and apparatus for anti-sorting particles is disclosed. In an example embodiment, particles move in a fluid along a microfluidic channel. A measurement device determines which particles are selected or desired particles by measuring a desired characteristic of the particles and outputting an associated signal. The energy source continuously imparts a force on unselected or undesired particles in a deflection region of the microfluidic channel to remove the unselected or undesired particles from the stream of particles. Based upon the signal outputted by the measurement device, the energy source is controlled to reduce or eliminate the force on selected particles such that the selected particles flow on a natural or expected flow path to a collection area such as an output channel. By avoiding application of force to desired particles, the anti-sorting systems and methods can improve viability and other characteristics of the desired particles.

公开(公告)号：US20240344963A1

公开(公告)日：2024-10-17

申请号：US18583173

申请日：2024-02-21

申请人： Life Technologies Corporation

发明人： Gregory Kaduchak ,  Michael Ward

IPC分类号： G01N15/1434 ,  B01L3/00 ,  G01J3/44 ,  G01N15/02 ,  G01N15/0227 ,  G01N15/14 ,  G01N15/1404 ,  G01N15/1429 ,  G01N21/53 ,  G01N21/64 ,  G01N15/00 ,  G01N15/10 ,  G01N15/149

CPC分类号： G01N15/1436 ,  B01L3/502761 ,  G01J3/4406 ,  G01N15/0227 ,  G01N15/0272 ,  G01N15/1404 ,  G01N15/1429 ,  G01N15/1434 ,  G01N15/1456 ,  G01N15/1459 ,  G01N15/1468 ,  G01N15/147 ,  G01N21/532 ,  G01N21/6456 ,  B01L2200/0652 ,  B01L2400/082 ,  G01N2015/0003 ,  G01N2015/0011 ,  G01N2015/03 ,  G01N2015/1006 ,  G01N2015/1028 ,  G01N2015/1415 ,  G01N2015/142 ,  G01N2015/1422 ,  G01N2015/1438 ,  G01N2015/1445 ,  G01N2015/1452 ,  G01N15/149

摘要： The present disclosure provides apparatuses, systems, and methods for performing particle analysis through flow cytometry at comparatively high event rates and for gathering high resolution images of particles.

公开(公告)号：US12044612B2

公开(公告)日：2024-07-23

申请号：US17712888

申请日：2022-04-04

申请人： Steven H. Reichenbach

发明人： Steven H. Reichenbach

IPC分类号： B01L3/00 ,  B01D43/00 ,  G01N15/10 ,  G01N15/01

CPC分类号： G01N15/1023 ,  B01D43/00 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2400/0487 ,  B01L2400/086 ,  G01N15/01 ,  G01N2015/1006 ,  G01N2015/1028

摘要： An apparatus and method to segregate particles suspended in a fluid with an obstacle field in the flow path of the fluid. The particles may be dispersed after an interaction with obstacles in the obstacle field. The obstacle-particle interactions may result in an asymmetrical particle shift or bump in which the particles are differentially dispersed relative to the obstacle and the fluid flow. Individual obstacles having properties that are asymmetrical are arranged creating asymmetrical gaps between adjacent pairs of obstacles to separate particles flowing through the device.

公开(公告)号：US20240228636A1

公开(公告)日：2024-07-11

申请号：US18481797

申请日：2023-10-05

申请人： Cellectis ,  Allogene Therapeutics, Inc.

发明人： Barbara Johnson SASU ,  Arvind RAJPAL ,  Philippe DUCHATEAU ,  Alexandre JUILLERAT ,  Julien VALTON

IPC分类号： C07K16/28 ,  A61K35/17 ,  A61K39/00 ,  A61K39/395 ,  C07K14/705 ,  C07K14/725 ,  C07K14/735 ,  C07K16/30 ,  C12N5/00 ,  C12N5/0783 ,  G01N15/01 ,  G01N15/10 ,  G01N15/1031 ,  G01N15/14 ,  G01N15/149

CPC分类号： C07K16/2866 ,  A61K35/17 ,  A61K39/0011 ,  A61K39/001176 ,  A61K39/39558 ,  C07K14/7051 ,  C07K14/70517 ,  C07K14/70535 ,  C07K14/70578 ,  C07K16/2803 ,  C07K16/2887 ,  C07K16/3061 ,  C12N5/0093 ,  C12N5/0636 ,  C12N5/0638 ,  G01N15/1031 ,  G01N15/14 ,  A61K2039/505 ,  A61K2039/5156 ,  A61K2039/5158 ,  A61K2039/6056 ,  A61K2039/64 ,  C07K2317/24 ,  C07K2317/53 ,  C07K2317/56 ,  C07K2317/622 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/03 ,  C12N2510/00 ,  G01N2015/016 ,  G01N2015/1006 ,  G01N2015/1028 ,  G01N15/149

摘要： A polypeptide encoding a chimeric antigen receptor (CAR) comprising at least one extracellular binding domain that comprises a scFv formed by at least a VH chain and a VL chain specific to an antigen, wherein said extracellular binding domain comprises at least one mAb-specific epitope.

公开(公告)号：US12020492B2

公开(公告)日：2024-06-25

申请号：US17105047

申请日：2020-11-25

申请人： SYSMEX CORPORATION

发明人： Momoko Imakubo ,  Jianyin Lu ,  Kentaro Shirai ,  Eri Katsumata ,  Yuji Okamoto

IPC分类号： G06V20/69 ,  G01N15/1429 ,  G01N15/1433 ,  G01N15/1434 ,  G06T7/00 ,  G06V10/70 ,  G01N15/00 ,  G01N15/10 ,  G01N15/14

CPC分类号： G06V20/69 ,  G01N15/1431 ,  G01N15/1433 ,  G01N15/1436 ,  G06T7/0012 ,  G06V10/87 ,  G01N2015/0019 ,  G01N2015/1028 ,  G01N2015/1495 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/20084 ,  G06T2207/30024

摘要： The present invention is to facilitate analysis of a plurality of analysis items. A cell analysis method for analyzing cells is provided in which data for analysis of cells contained in a sample are generated, and an artificial intelligence algorithm to be the input destination of the generated analysis data is selected from among a plurality of artificial intelligence algorithms, data indicating the properties of the cells are generated based on the analysis data via the artificial intelligence algorithm.

公开(公告)号：US20240192109A1

公开(公告)日：2024-06-13

申请号：US18550871

申请日：2022-03-18

申请人： NANYANG TECHNOLOGICAL UNIVERSITY

发明人： Han Wei HOU ,  Chayakorn PETCHAKUP ,  King Ho Holden LI

IPC分类号： G01N15/02 ,  B01L3/00 ,  G01N15/10 ,  G01N15/1031

CPC分类号： G01N15/0266 ,  B01L3/502761 ,  B01L3/502776 ,  G01N15/1023 ,  G01N15/1031 ,  B01L2200/0652 ,  B01L2300/0645 ,  B01L2300/0663 ,  B01L2400/0487 ,  G01N2015/1006 ,  G01N2015/1028 ,  G01N2015/1029

摘要： The present invention relates to a microfluidic impedance cytometer comprising an integrated biosensor and use of the device thereof for label-free activation profiling of cells in a sample, such as myelocytes and/or lymphocytes and neutrophils. The microfluidic impedance cytometer may comprise a spiral-shaped flow channel, coplanar electrodes for generating an electric field across the channel, a first sample inlet and a second sheath fluid inlet; wherein the sample inlet is a stepped sample inlet in that the height of the sample inlet is lower than the height of the sheath fluid inlet and the flow channel.

公开(公告)号：US20240165623A1

公开(公告)日：2024-05-23

申请号：US18281514

申请日：2022-03-11

申请人： Qatar Foundation for Education, Science and Community Development

发明人： Ali Sultan ,  Sini Skariah ,  Arum Han ,  Adrian Guzman

IPC分类号： B01L3/00 ,  G01N15/01 ,  G01N15/10

CPC分类号： B01L3/502784 ,  B01L3/502707 ,  G01N15/01 ,  G01N15/10 ,  B01L2200/0652 ,  B01L2200/0673 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/12 ,  G01N2015/1028

摘要： A method and an integrated device are provided for high-throughput screening of cellular libraries utilizing a droplet microfluidic-based approach. The integrated device comprises 8 or more major functionalities including droplet generation, droplet incubation, droplet reflow, droplet cleaving/generation, droplet synchronization, droplet merging, droplet detection, and droplet sorting for complex screening assays. Integration of each of the droplet functionalities onto a single chip reduces drastic changes in flow experienced at various chip-to-chip interfaces, and the possibility of error.

公开(公告)号：US20240142369A1

公开(公告)日：2024-05-02

申请号：US18278067

申请日：2022-02-22

申请人： Sony Group Corporation

发明人： Taiki Uno ,  Tatsumi Ito

IPC分类号： G01N15/14 ,  B01L3/00

CPC分类号： G01N15/1484 ,  B01L3/502761 ,  G01N2015/1028

摘要： There is provided a particle sorting kit including a filter unit, which has a small amount of particle loss at a connection portion between a tube through which a sample liquid flows and the filter unit and functions even at a low flow rate.
There is provided a particle sorting kit including a sample accommodation unit for accommodating a sample liquid containing particles, a sample flow path through which the sample liquid flows, a detection region in which a target particle is detected from the sample liquid, and a filter unit including a filter and a fitting portion that fits to an outer diameter of a tube for connection with the sample accommodation unit and/or the sample flow path, in which the fitting portion includes a protrusion that protrudes in the tube direction.