公开(公告)号：US07202945B2

公开(公告)日：2007-04-10

申请号：US10878895

申请日：2004-06-28

Applicant: Andreas Erlbacher ,  Lutz Niggl ,  Alois Krutzenbichler

Inventor： Andreas Erlbacher ,  Lutz Niggl ,  Alois Krutzenbichler

IPC: G01N21/03

CPC classification number: G01N21/6452 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/168 ,  G01N21/0303 ,  G01N21/253 ,  G01N21/59 ,  G01N2021/6463 ,  G01N2021/6491 ,  G01N2021/651 ,  G01N2201/0446 ,  G01N2201/1042 ,  Y10S436/809

Abstract: The present invention relates to a device (1) and a method for determining parameters of fluid-containing samples (2) in a system (3) for individually irradiating the samples (2) with light (4) of a light source (5) in an essentially vertical irradiation direction (6). In this case, this system (3) includes a detector (7) for measuring the light (8) coming from a single sample, and this detector (7) has a detection direction (9′), which lies on an optical axis (9) that is essentially parallel to the optical axis (6) of the light source (5). This device (1) includes at least one reflective surface (10), using which the light (4) coming essentially vertically out of the light source (5) may be at least partially deflected in an essentially horizontal irradiation direction (11). The device according to the present invention and the method according to the present invention are distinguished in that the detection direction (9′) of the detector (7)—for measuring the individual light (8) coming from a single sample (2)—is positioned at an angle to the optical axis of the light (4) irradiating the sample (2) in such a way that only the light (8) coming from the individual sample (2), but not this light (4), reaches the detector (7).

Abstract translation: 本发明涉及一种用于在系统（3）中确定用于用光源（5）的光（4）单独照射样品（2）的系统（3）中的含液样品（2）的参数的装置（1）和方法， 在基本垂直的照射方向（6）上。 在这种情况下，该系统（3）包括用于测量来自单个样品的光（8）的检测器（7），并且该检测器（7）具有位于光轴（9'）上的检测方向（9' 9），其基本上平行于光源（5）的光轴（6）。 该装置（1）包括至少一个反射表面（10），基本上垂直离开光源（5）的光线可以在基本上水平的照射方向（11）上至少部分地偏转。 根据本发明的装置和根据本发明的方法的特征在于，用于测量来自单个样品（2）的单个光（8）的检测器（7）的检测方向（9'） - 被定位成与照射样品（2）的光（4）的光轴成一定角度，使得仅来自各个样品（2）的光（8）而不是该光（4）到达 检测器（7）。

公开(公告)号：US20030222222A1

公开(公告)日：2003-12-04

申请号：US10160251

申请日：2002-06-04

Applicant: National Taiwan University

Inventor： Chen-Yuan Dong ,  Peter T.C. So ,  Sunney I. Chan

IPC: G01N021/64

CPC classification number: G01N21/6452 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2201/0446 ,  G01N2201/0453

Abstract: This invention provides a multiphoton excitaion microscope for simultaneously detecting differently colored fluorescence materials on biochips, which includes a multiphoton excitation source, objectives, and a plurality of detection channels. The biochip is hybridized and labeled with fluorescence materials for expressing hybridized biological signals. The multiphoton excitation source is focused to a light spot on the biochip to excite the fluorescence materials bound thereon. After that, the fluorescence emission at different wavelengths from the different fluorescent materials can be detected by the plural detection channels.

Abstract translation: 本发明提供了一种多光子激发显微镜，用于同时检测生物芯片上的不同颜色的荧光材料，其包括多光子激发源，物镜和多个检测通道。 将生物芯片杂交并用用于表达杂交的生物信号的荧光材料标记。 多光子激发源被聚焦到生物芯片上的光点以激发结合在其上的荧光材料。 之后，可以通过多个检测通道检测来自不同荧光材料的不同波长的荧光发射。

公开(公告)号：US6151111A

公开(公告)日：2000-11-21

申请号：US929552

申请日：1997-09-15

Applicant: Mark Wechsler ,  Howard H. Barney ,  Roger A. Kaye ,  David G. Ogle ,  Michael M. Lacy ,  Calvin Y. Chow ,  Kimberly L. Crawford ,  Dean G. Hafeman

Inventor： Mark Wechsler ,  Howard H. Barney ,  Roger A. Kaye ,  David G. Ogle ,  Michael M. Lacy ,  Calvin Y. Chow ,  Kimberly L. Crawford ,  Dean G. Hafeman

IPC: G01N21/33 ,  G01N21/25 ,  G01N21/31 ,  G01N21/59 ,  G01N35/02 ,  G01J3/30

CPC classification number: G01N21/33 ,  G01N21/253 ,  G01N21/31 ,  G01N2201/0231 ,  G01N2201/0446 ,  G01N2201/1222 ,  G01N2201/127 ,  G01N35/028

Abstract: The present invention relates to a photometric device for measuring optical parameters. The invention functions in the ultraviolet light range through use of a monochromator and splits the test light in multiple channels by a rotor assembly, including a mirror.

公开(公告)号：US5345395A

公开(公告)日：1994-09-06

申请号：US785800

申请日：1991-10-31

Applicant: Christopher D. Griner

Inventor： Christopher D. Griner

IPC: G01N21/17 ,  G01N21/25 ,  G01N21/27 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10

CPC classification number: G01N21/253 ,  G01N21/274 ,  G01N35/028 ,  G01N2201/0407 ,  G01N2201/0446 ,  G01N2201/08 ,  G01N2201/1283

Abstract: A system uses a photometer that establishes an optical channel between a light emitter and a light detector. The photometer analyses a specimen while it occupies a well in a tray. A movable platform transports the tray to the optical channel for analysis and removes the tray from the optical channel after analysis. The system calibrates the movable platform to assure proper alignment between the specimen and the photometric apparatus used to analyzed the specimen. The system also calibrates the optical channel without use of an external reference.

Abstract translation: 系统使用在光发射器和光检测器之间建立光通道的光度计。 光度计分析样品时，它在托盘中占有很好的位置。 可移动平台将托盘运送到光通道进行分析，并在分析后从光通道中移除托盘。 系统校准可移动平台，以确保样品与用于分析样品的光度测量仪器之间的正确对准。 该系统还可以校准光通道而不使用外部参考。

公开(公告)号：US5325295A

公开(公告)日：1994-06-28

申请号：US688220

申请日：1991-04-22

Applicant: Joseph C. Fratantoni ,  Betty J. Poindexter

Inventor： Joseph C. Fratantoni ,  Betty J. Poindexter

IPC: G01N15/00 ,  G01N21/82 ,  G06F15/00 ,  G01N33/48

CPC classification number: G01N21/82 ,  G01N2015/0084 ,  G01N2201/0407 ,  G01N2201/0446

Abstract: A rapid method for the simultaneous measurement of aggregation or agglutination in plurality of microtest wells or other sample-holding vessels comprises providing a plurality of light beams having discrete focal points adapted to be focused on the contents of a plurality of wells or other vessels and a plurality of photodetectors therefor, directing the light beams through the wells or other vessels and contents thereof, continuously agitating the wells or other vessels and contents thereof with a circular movement effective to permit aggregation or agglutination while preserving the integrity of the material, producing a plurality of output signals from the detectors are proportional to the light absorbencies of the beams as they pass through the wells or other vessels and contents thereof, repeatedly sampling the signals at a predetermined time interval and determining changes in optical densities from the signals obtained after each predetermined time interval by comparing each signal sampled after each predetermined time interval with the first signal sampled for the same microtest well or vessel, whereby any change in optical density which is greater than about 0.05 units is said to indicate that aggregation or agglutination occurred in the well or other vessel. The present method is suitably applied to the measurement of aggregation in platelets.

Abstract translation: 用于同时测量多个微测井或其他样品保持容器中的聚集或凝集的快速方法包括提供多个光束，其具有适于聚焦在多个孔或其它容器的内容物上的离散焦点，以及 多个光电检测器，用于引导光束通过孔或其它容器及其内容物，以圆周运动连续地搅动孔或其它容器及其内容物，以有效地允许聚集或凝集，同时保持材料的完整性，产生多个 来自检测器的输出信号与光束在通过孔或其他容器及其内容物时的光吸收率成比例，以预定的时间间隔重复采样信号，并根据每个预定值之后获得的信号确定光密度的变化 通过比较每个信号的时间间隔 在每个预定时间间隔之后采样，对于相同的微测井或容器采样第一信号，由此大于约0.05单位的光密度的任何变化据说表明孔或其它容器中发生聚集或凝集。 本方法适用于血小板聚集的测定。

公开(公告)号：US5290513A

公开(公告)日：1994-03-01

申请号：US914742

申请日：1992-07-20

Applicant: Fritz Berthold ,  Willy Lohr

Inventor： Fritz Berthold ,  Willy Lohr

IPC: G01N21/64 ,  G01N21/75 ,  G01N21/76 ,  G01N35/02 ,  G01N35/10

CPC classification number: G01N35/028 ,  G01N21/76 ,  G01N2035/1062 ,  G01N2201/0446 ,  G01N2201/0646 ,  G01N2201/08

Abstract: A radiation measuring apparatus for performing measurements on samples contained in a plurality of sample containers disposed in the form of a matrix on a holder plate, the apparatus including a radiation detector having an entrance window, displacement devices for aligning successive sample containers with the entrance window of the radiation detector for automatic measurement of samples in the sample containers, the radiation detector and the sample containers being movable relative to one another in such a way that the sample container being measured and the entrance window of the detector are disposed coaxially with each other. The apparatus further includes: a diaphragm plate (10) provided with at least one aperture, the diaphragm plate being fixedly disposed between the entrance window (31) of the detector (30) and a sample container (21) placed underneath the entrance window; a component forming a light-tight coupling between a sample container (21) placed underneath the entrance window and the entrance window; and a component disposed for pressing the sample containers against the diaphragm plate (10) with a constant pressure while permitting the sample containers to be displaced parallel to the diaphragm plate by the displacement devices.

Abstract translation: 一种辐射测量装置，用于对包含在矩阵形式的多个样品容器中的样品进行测量，所述样品容器设置在保持板上，所述装置包括具有入口窗的辐射检测器，用于将连续的样品容器与入口窗口对准的位移装置 的放射线检测器，用于自动测量样品容器中的样品，辐射检测器和样品容器可相对于彼此移动，使得被测量的样品容器和检测器的入口窗口彼此同轴设置 。 该装置还包括：设置有至少一个孔的隔膜板（10），该隔膜板固定地设置在检测器（30）的入口窗（31）和置于入口窗下方的样品容器（21）之间; 形成在入口窗下方的样本容器（21）与入口窗口之间的不透光耦合的部件; 以及用于以一定压力将样本容器压靠在隔膜板（10）上的部件，同时允许样品容器通过位移装置平行于隔膜板移位。

公开(公告)号：US5275951A

公开(公告)日：1994-01-04

申请号：US939570

申请日：1992-09-03

Applicant: Herbert S. Chow ,  John J. Kotlarik ,  Mieczyslaw Wroblewski ,  Thomas J. Wilson ,  Jimmy D. McCoy

Inventor： Herbert S. Chow ,  John J. Kotlarik ,  Mieczyslaw Wroblewski ,  Thomas J. Wilson ,  Jimmy D. McCoy

IPC: G01N21/17 ,  B01L3/00 ,  B01L3/02 ,  G01N15/14 ,  G01N21/82 ,  G01N33/48 ,  G01N33/483 ,  G01N33/543 ,  G01N33/80 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10 ,  G02B7/28 ,  G06T1/00 ,  G01F23/00 ,  G01F23/28

CPC classification number: G01N35/109 ,  B01L3/0262 ,  B01L3/5085 ,  G01N15/1468 ,  G01N21/82 ,  G01N35/0095 ,  G01N35/0098 ,  G01N35/028 ,  G02B7/28 ,  G01N2035/042 ,  G01N2035/0425 ,  G01N2035/0491 ,  G01N2035/1018 ,  G01N2035/1025 ,  G01N2035/1034 ,  G01N2201/0446 ,  G01N2201/0461 ,  G01N2201/0492 ,  G01N35/1004 ,  Y10T137/7306 ,  Y10T436/115831

Abstract: An apparatus is provided which includes a reaction cartridge having a plurality of reaction wells having different reagents disposed thereon, at least one well adapted to receive a sample, a well containing particles adapted to bind to the sample and which have the capability of being separated from cells which are not bound to the separation particles and at least one fluorophore adapted to bind to a specific type of cell in the sample, and a wash area adapted for washing a probe. An image forming device is provided to detect images which indicate whether specific reactions have occurred in each of the reaction wells. The apparatus also includes a mechanism for dispensing and aspirating liquids including a mechanism for detecting liquid levels. The device further includes logic for analyzing the information received from the image detection apparatus and processing the information to generate a visual indication of the results of the assays being performed. A microprocessor is provided to assist in the operation of the device as well as in the image processing.

Abstract translation: 提供了一种装置，其包括具有多个反应孔的反应筒，反应孔具有设置在其上的不同试剂，至少一个适于接收样品的孔，含有适于结合样品的颗粒的孔，并具有与样品分离的能力 不结合分离颗粒的细胞和至少一种适于结合样品中特定类型的细胞的荧光团和适于洗涤探针的洗涤区域。 提供图像形成装置以检测指示每个反应孔中是否发生特定反应的图像。 该装置还包括用于分配和抽吸液体的机构，包括用于检测液位的机构。 该装置还包括用于分析从图像检测装置接收的信息并处理信息以生成正在执行的测定结果的视觉指示的逻辑。 提供微处理器以帮助设备的操作以及图像处理。

公开(公告)号：US5183761A

公开(公告)日：1993-02-02

申请号：US383938

申请日：1989-07-21

Applicant: Mary J. Freeman ,  Gary M. Freeman

Inventor： Mary J. Freeman ,  Gary M. Freeman

IPC: G01N21/27

CPC classification number: G01N21/278 ,  G01N2201/0446 ,  Y10T436/10 ,  Y10T436/109163

Abstract: A method and device for calibrating or checking the calibration of vertical photometers. The invention may also provide easy and convenient assay results for diagnostic profiles. The invention comprises pre-dispensed amounts of a suitable reagent, chosen for the particular use, disposed in a vessel with an amount of a wetting agent to yield a reproducible meniscus in the resulting control solution. The control solution is dried to leave a precise amount of dried reagent and other constituents in the vessel which can then be stored and distributed until subsequent use. The dried control solution can then be reconstituted to provide a standard having known absorbance and physical characteristics.

Abstract translation: 用于校准或检查垂直光度计校准的方法和装置。 本发明还可以为诊断特征提供简单和方便的测定结果。 本发明包括预先分配量的选择用于特定用途的合适的试剂，其设置在具有一定量的润湿剂的容器中，以在所得对照溶液中产生可再现的弯液面。 将对照溶液干燥以在容器中留下精确量的干燥试剂和其它成分，然后将其存储和分配直至随后使用。 然后可以将干燥的对照溶液重构以提供具有已知吸光度和物理特性的标准品。

公开(公告)号：US5159197A

公开(公告)日：1992-10-27

申请号：US567383

申请日：1990-08-14

Applicant: Jon C. Wannlund

Inventor： Jon C. Wannlund

IPC: B01L3/00 ,  C12M1/20 ,  G01N21/03 ,  G01N21/76

CPC classification number: B01L3/5025 ,  B01L3/50255 ,  G01N21/03 ,  G01N21/76 ,  G01N2021/0325 ,  G01N2021/0328 ,  G01N2021/0357 ,  G01N2021/1768 ,  G01N2201/0446

Abstract: Apparatus for performing and measuring chemical reactions includes a reaction test apparatus having reaction wells wherein reactants are controllably mixed, and exposure apparatus which receives and positions the reaction test apparatus adjacent a photographic film. Each of the reaction wells includes at least two reaction cups, arranged one above the other. The uppermost reaction cups have orifices in the bottoms, so that liquid can be mixed and reacted in the uppermost cup, and then controllably transferred to the lower cup to be mixed with additional reactants. In a preferred embodiment, the reaction cups are supported in plates that are structurally integrated with the cups, and are superimposed to make a test block. The test block is retained in the exposure apparatus, and liquid is forced from the upper cup to the lower cup by application of pressure to the top of the upper cup. The apparatus of the invention is particularly suited for measuring reactions that produce luminescence of short duration, as the reactants can be conveniently mixed in darkness, while the film is being exposed.

Abstract translation: 用于进行和测量化学反应的装置包括具有可反应混合反应物的反应孔的反应试验装置，以及将反应试验装置接收并定位在照相胶片附近的曝光装置。 每个反应孔包括至少两个反应杯，一个彼此排列。 最上面的反应杯在底部具有孔口，使得液体可以在最上面的杯子中混合和反应，然后可控制地转移到下部杯中以与另外的反应物混合。 在优选实施例中，反应杯被支撑在结构上与杯子一体化的板中，并且叠加以制成测试块。 测试块被保留在曝光设备中，并且通过向上杯的顶部施加压力将液体从上杯强制到下杯。 本发明的装置特别适用于测量产生短持续时间的发光的反应，因为反射剂可以在黑暗中方便地混合，同时曝光该膜。

公开(公告)号：US5073029A

公开(公告)日：1991-12-17

申请号：US481450

申请日：1990-02-16

Applicant: James P. Eberly ,  Ralph A. Magnotti, Jr.

Inventor： James P. Eberly ,  Ralph A. Magnotti, Jr.

IPC: G01N21/27 ,  G01N21/25 ,  G01N21/77

CPC classification number: G01N21/253 ,  G01N21/255 ,  G01N2201/0407 ,  G01N2201/0446 ,  G01N2201/0621

Abstract: An optoelectronic device is provided which permits rapid sequential measurement of the optical density of multiple samples. The device employs a plurality of light-emitting diodes (LEDs) as light sources and a plurality of photodiodes as light detectors. In a preferred embodiment, arrays of LEDs paired with photodiodes are adapted to measure the optical density of samples contained in multiwell plastic plates (microplates) conventionally used for immunoassays.

Abstract translation: 提供了一种允许快速连续测量多个样品的光密度的光电子器件。 该装置使用多个发光二极管（LED）作为光源和多个光电二极管作为光检测器。 在优选实施例中，与光电二极管配对的LED阵列适于测量通常用于免疫测定的多孔塑料板（微孔板）中包含的样品的光密度。