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    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS
    244.
    发明申请
    MULTI-PRIMER AMPLIFICATION METHOD FOR BARCODING OF TARGET NUCLEIC ACIDS 审中-公开
    用于靶向靶核酸的多重PCR扩增方法

    公开(公告)号：US20140272952A1

    公开(公告)日：2014-09-18

    申请号：US14180262

    申请日：2014-02-13

    Applicant: Fluidigm Corporation

    Inventor： Andrew May ,  Peilin Chen ,  Jun Wang ,  Fiona Kaper ,  Megan Anderson

    IPC: C12Q1/68

    CPC classification number: C12Q1/6806 ,  B01L3/50273 ,  B01L3/502738 ,  B01L7/52 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  B01L2400/0655 ,  C12Q1/686 ,  C12Q2525/155 ,  C12Q2525/161 ,  C12Q2527/143 ,  C12Q2535/122 ,  C12Q2549/119 ,  C12Q2563/179 ,  C12Q2565/629

    Abstract: In certain embodiments, the present invention provides amplification methods in which nucleotide tag(s) and, optionally, a barcode nucleotide sequence are added to target nucleotide sequences. In other embodiments, the present invention provides a microfluidic device that includes a plurality of first input lines and a plurality of second input lines. The microfluidic device also includes a plurality of sets of first chambers and a plurality of sets of second chambers. Each set of first chambers is in fluid communication with one of the plurality of first input lines. Each set of second chambers is in fluid communication with one of the plurality of second input lines. The microfluidic device further includes a plurality of first pump elements in fluid communication with a first portion of the plurality of second input lines and a plurality of second pump elements in fluid communication with a second portion of the plurality of second input lines.

    Abstract translation: 在某些实施方案中，本发明提供了其中将核苷酸标签和任选的条形码核苷酸序列加入靶核苷酸序列的扩增方法。 在其它实施例中，本发明提供一种包括多个第一输入线和多个第二输入线的微流体装置。 微流体装置还包括多组第一腔室和多组第二腔室。 每组第一腔室与多个第一输入管线中的一个流体连通。 每组第二腔室与多个第二输入管线中的一个流体连通。 微流体装置还包括与多个第二输入线的第一部分流体连通的多个第一泵元件和与多个第二输入线的第二部分流体连通的多个第二泵元件。

    SURFACE CHEMISTRY AND DEPOSITION TECHNIQUES
    245.
    发明申请
    SURFACE CHEMISTRY AND DEPOSITION TECHNIQUES 有权
    表面化学和沉积技术

    公开(公告)号：US20140256561A1

    公开(公告)日：2014-09-11

    申请号：US14092728

    申请日：2013-11-27

    Applicant: Jerrod Schwartz ,  Stephen R. Quake ,  Milan Mrksich

    Inventor： Jerrod Schwartz ,  Stephen R. Quake ,  Milan Mrksich

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  G01N33/54353 ,  G01N33/54393 ,  Y10T436/143333

    Abstract: Surface chemistries for the visualization of labeled single molecules (analytes) with improved signal-to-noise properties are provided. To be observed, analyte molecules are bound to surface attachment features that are spaced apart on the surface such that when the analytes are labeled adjacent analytes are optically resolvable from each other. One way to express this concept is that binding elements should be spaced apart such that the Guassian point spread functions of adjacent labels do not overlap. Another way of expressing this concept is that the surface binding elements should be spaced apart by a distance equal to at least the diffraction limit for an optical label attached to the bound analytes.

    Abstract translation: 提供了具有改进的信噪比特性的标记单分子（分析物）可视化的表面化学性质。 为了观察，分析物分子结合在表面上间隔开的表面附着特征，使得当分析物被标记为相邻分析物时，彼此是可光学解析的。 表达这个概念的一种方式是绑定元素应该间隔开，使得相邻标签的古斯分布函数不重叠。 表达这个概念的另一种方式是，表面结合元件应该间隔至少等于连接到结合分析物的光学标签的衍射极限的距离。

    SINGLE-CELL NUCLEIC ACID ANALYSIS
    246.
    发明申请
    SINGLE-CELL NUCLEIC ACID ANALYSIS 审中-公开
    单细胞核酸分析

    公开(公告)号：US20140193812A1

    公开(公告)日：2014-07-10

    申请号：US14102331

    申请日：2013-12-10

    Applicant: Fluidigm Corporation

    Inventor： Amy Hamilton ,  Min Lin ,  Alain Mir ,  Martin Pieprzyk

    IPC: C12Q1/68

    CPC classification number: C12Q1/6881 ,  C12Q1/6844 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2531/119 ,  C12Q2531/137 ,  C12Q2565/101 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2600/178

    Abstract: The present invention provides methods for analysis of genomic DNA and/or RNA from small samples or even single cells. Methods for analyzing genomic DNA can entail whole genome amplification (WGA), followed by preamplification and amplification of selected target nucleic acids. Methods for analyzing RNA can entail reverse transcription of the desired RNA, followed by preamplification and amplification of selected target nucleic acids.

    Abstract translation: 本发明提供从小样品甚至单细胞分析基因组DNA和/或RNA的方法。 分析基因组DNA的方法可能需要全基因组扩增（WGA），然后对选定的靶核酸进行预扩增和扩增。 分析RNA的方法可能需要所需RNA的逆转录，然后对选定的靶核酸进行预扩增和扩增。

    METHODS AND SYSTEMS FOR IMAGE PROCESSING OF MICROFLUIDIC DEVICES
    247.
    发明申请
    METHODS AND SYSTEMS FOR IMAGE PROCESSING OF MICROFLUIDIC DEVICES 有权
    微流体装置图像处理方法与系统

    公开(公告)号：US20140133732A1

    公开(公告)日：2014-05-15

    申请号：US14071423

    申请日：2013-11-04

    Applicant: Fluidigm Corporation

    Inventor： Simant Dube ,  Gang Sun ,  Lian-She Zhao

    IPC: G01N21/64

    CPC classification number: G01N21/64 ,  G06T7/0012 ,  G06T7/74 ,  G06T2207/10064 ,  G06T2207/30004

    Abstract: A method of processing data associated with fluorescent emissions from a microfluidic device. The method includes performing an auto-focus process associated with a first image of the microfluidic device and performing an auto-exposure process associated with the first image of the microfluidic device. The method also includes capturing a plurality of images of the microfluidic device. The plurality of images are associated with a plurality of thermal cycles. The method further includes performing image analysis of the plurality of captured images to determine a series of optical intensities and performing data analysis of the series of optical intensities to provide a series of change in threshold values.

    Abstract translation: 处理与微流体装置的荧光发射有关的数据的方法。 该方法包括执行与微流体装置的第一图像相关联的自动聚焦处理，并且执行与微流体装置的第一图像相关联的自动曝光处理。 该方法还包括捕获微流体装置的多个图像。 多个图像与多个热循环相关联。 该方法还包括执行多个拍摄图像的图像分析以确定一系列光强度并执行该系列光强度的数据分析以提供一系列阈值变化。

    Optical lens system and method for microfluidic devices
    248.
    发明授权
    Optical lens system and method for microfluidic devices 有权
    用于微流体装置的光学透镜系统和方法

    公开(公告)号：US08721968B2

    公开(公告)日：2014-05-13

    申请号：US14031972

    申请日：2013-09-19

    Applicant: Fluidigm Corporation

    Inventor： Marc A. Unger ,  Geoffrey Richard Facer ,  Barry Clerkson ,  Christopher G. Cesar ,  Neil Switz

    IPC: G01N21/00 ,  G01N15/06 ,  G01N21/84

    CPC classification number: C12Q1/686 ,  B01J2219/00576 ,  B01J2219/00704 ,  B01L3/5027 ,  B01L7/52 ,  G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6486 ,  G01N21/75 ,  G01N21/8483 ,  G01N27/44721 ,  G01N2021/6421 ,  G01N2201/061 ,  G02B21/16 ,  G02B21/36

    Abstract: An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber.

    Abstract translation: 一种用于从微流体装置成像一个或多个选定的荧光指示的装置。 该装置包括耦合到至少一个微流体装置中的至少一个室的成像路径。 成像路径提供从至少一个微流体装置的至少一个室中的一个或多个样品衍生的一个或多个荧光发射信号的传输。 腔室具有腔室尺寸，腔室尺寸的特征在于与成像路径垂直的实际空间尺寸。 该装置还包括耦合到成像路径的光学透镜系统。 光学透镜系统适于透射与腔室相关联的一个或多个荧光信号。

    Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags
    249.
    发明授权
    Methods for detecting multiple target nucleic acids in multiple samples by use nucleotide tags 有权
    通过使用核苷酸标签检测多个样品中的多个靶核酸的方法

    公开(公告)号：US08697363B2

    公开(公告)日：2014-04-15

    申请号：US12548132

    申请日：2009-08-26

    Applicant: Alain Mir ,  Ramesh Ramakrishnan ,  Marc Unger ,  Bernhard G. Zimmermann

    Inventor： Alain Mir ,  Ramesh Ramakrishnan ,  Marc Unger ,  Bernhard G. Zimmermann

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12N15/1065 ,  C12Q1/6853 ,  Y10T436/143333 ,  C12Q2537/143 ,  C12Q2525/161

    Abstract: The present invention provides assay methods that increase the number of samples and/or target nucleic acids that can be analyzed in a single assay. In certain embodiments, an assay method entails separately subjecting S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence including a sample-specific nucleotide tag and a target nucleotide sequence. In some embodiments, an assay method entails separately subjecting S samples to an encoding reaction that produces a set of T tagged target nucleotide sequences, each tagged target nucleotide sequence including a first nucleotide tag linked to a target nucleotide sequence, which is linked to a second nucleotide tag. In either case, the tagged target nucleotide sequences from the S samples can be mixed to form an assay mixture and subsequently assayed.

    Abstract translation: 本发明提供了增加可以在单次测定中分析的样品和/或靶核酸数量的测定方法。 在某些实施方案中，测定方法需要分别使S样品经受产生一组T标记的靶核苷酸序列的编码反应，每个标记的靶核苷酸序列包括样品特异性核苷酸标签和靶核苷酸序列。 在一些实施方案中，测定方法需要分别使S样品经受产生一组T标记的靶核苷酸序列的编码反应，每个标记的靶核苷酸序列包括与靶核苷酸序列连接的第一个核苷酸标签，其连接到第二个 核苷酸标签。 在任一情况下，可以将来自S样品的标记的靶核苷酸序列混合以形成测定混合物并随后测定。

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