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    Organisms for the production of cyclohexanone
    267.
    发明授权
    Organisms for the production of cyclohexanone 有权
    用于生产环己酮的生物体

    公开(公告)号：US09139853B2

    公开(公告)日：2015-09-22

    申请号：US14172079

    申请日：2014-02-04

    Applicant: Genomatica, Inc.

    Inventor： Robin E. Osterhout ,  Anthony P. Burgard ,  Mark J. Burk ,  Priti Pharkya

    IPC: C12N9/16 ,  C12N9/88 ,  C12N1/20 ,  C12P7/26 ,  C12N9/00 ,  C12N15/52 ,  C12N15/70

    CPC classification number: C12P7/26 ,  C12N9/16 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12N15/70

    Abstract: A non-naturally occurring microbial organism has cyclohexanone pathways that include at least one exogenous nucleic acid encoding a cyclohexanone pathway enzyme. A pathway includes a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on C—C bond), a 2-ketocyclohexane-1-carboxylate decarboxylase and an enzyme selected from a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxyl-CoA transferase, and a 2-ketocyclohexane-1-carboxyl-CoA synthetase. A pathway includes an enzyme selected from a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on C—C bond), a 6-ketocyclohex-1-ene-1-carboxyl-CoA synthetase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA hydrolase (acting on thioester), a 6-ketocyclohex-1-ene-1-carboxyl-CoA transferase, a 6-ketocyclohex-1-ene-1-carboxyl-CoA reductase, a 6-ketocyclohex-1-ene-1-carboxylate decarboxylase, a 6-ketocyclohex-1-ene-1-carboxylate reductase, a 2-ketocyclohexane-1-carboxyl-CoA synthetase, a 2-ketocyclohexane-1-carboxyl-CoA transferase, a 2-ketocyclohexane-1-carboxyl-CoA hydrolase (acting on thioester), a 2-ketocyclohexane-1-carboxylate decarboxylase, and a cyclohexanone dehydrogenase. A pathway includes an adipate semialdehyde dehydratase, a cyclohexane-1,2-diol dehydrogenase, and a cyclohexane-1,2-diol dehydratase. A pathway includes a 3-oxopimelate decarboxylase, a 4-acetylbutyrate dehydratase, a 3-hydroxycyclohexanone dehydrogenase, a 2-cyclohexenone hydratase, a cyclohexanone dehydrogenase and an enzyme selected from a 3-oxopimeloyl-CoA synthetase, a 3-oxopimeloyl-CoA hydrolase (acting on thioester), and a 3-oxopimeloyl-coA transferase. Each these pathways can include a PEP carboxykinase. A method for producing cyclohexanone includes culturing these non-naturally occurring microbial organisms.

    Abstract translation: 非天然存在的微生物有机体具有包含至少一种编码环己酮途径酶的外源核酸的环己酮途径。 途径包括2-酮环己烷-1-羧基-CoA水解酶（作用于C-C键），2-酮环己烷-1-羧酸脱羧酶和选自2-酮环己烷-1-羧基-CoA水解酶的酶（作用 在硫酯上），2-酮环己烷-1-羧基-CoA转移酶和2-酮环己烷-1-羧基-CoA合成酶。 途径包括选自6-酮环己-1-烯-1-羧基-CoA水解酶（作用于C-C键）的酶，6-酮环己-1-烯-1-羧基-CoA合成酶，6- 酮酮环己-1-烯-1-羧基-CoA水解酶（作用于硫酯），6-酮环己-1-烯-1-羧基-CoA转移酶，6-酮环己-1-烯-1-羧基-CoA还原酶， 6-酮环己-1-烯-1-羧酸脱羧酶，6-酮环己-1-烯-1-羧酸还原酶，2-酮环己烷-1-羧基-CoA合成酶，2-酮环己烷-1-羧基-CoA 转移酶，2-酮环己烷-1-羧基-CoA水解酶（作用于硫酯），2-酮环己烷-1-羧酸脱羧酶和环己酮脱氢酶。 途径包括己二酸半醛脱水酶，环己烷-1,2-二醇脱氢酶和环己烷-1,2-二醇脱水酶。 途径包括3-氧代邻苯二甲酸脱羧酶，4-乙酰基丁酸脱水酶，3-羟基环己酮脱氢酶，2-环己烯酮水合酶，环己酮脱氢酶和选自3-氧代木糖酰辅酶A合成酶，3-氧代木糖酰-CoA水解酶 （作用于硫酯）和3-氧代木糖酰辅酶A转移酶。 每个这些途径可以包括PEP羧基激酶。 生产环己酮的方法包括培养这些非天然存在的微生物。

    Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol
    269.
    发明授权
    Methods and organisms for utilizing synthesis gas or other gaseous carbon sources and methanol 有权
    利用合成气或其他气态碳源和甲醇的方法和生物

    公开(公告)号：US09051552B2

    公开(公告)日：2015-06-09

    申请号：US12875068

    申请日：2010-09-02

    Applicant: Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

    Inventor： Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

    IPC: C12N9/20 ,  C12N9/10 ,  C12N9/02 ,  C12N9/00 ,  C12P19/32

    CPC classification number: C12P7/18 ,  C12N9/0008 ,  C12N9/0036 ,  C12N9/1007 ,  C12N9/93 ,  C12N15/70 ,  C12P19/32 ,  Y02E50/343

    Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.

    Abstract translation: 本发明提供具有乙酰-CoA途径的非天然存在的微生物生物体，以及利用合成气或合成气和甲醇的能力。 在一个实施方案中，本发明提供非天然存在的微生物，其包含一种或多种外源蛋白质，赋予微生物将CO，CO 2和/或H 2转化为乙酰辅酶A（乙酰辅酶A），甲基四氢叶酸（甲基 -THF）或其它所需产物，其中微生物缺乏在不存在一种或多种外源蛋白的情况下将CO或CO 2和H 2转化为乙酰辅酶A或甲基-THF的能力。 例如，微生物生物体可以含有至少一种编码乙酰辅酶A途径中的酶或蛋白质的外源核酸。 微生物生物能够单独或与甲醇组合使用包含CO，CO 2和/或H 2的合成气体，以产生乙酰辅酶A。 本发明另外提供了生产乙酰辅酶A的方法，例如通过培养产生乙酰辅酶A的微生物，其中微生物生物体表达至少一种编码乙酰辅酶A途径酶或蛋白质的外源核酸，其量足够 在条件下和足够的时间内产生乙酰辅酶A产生乙酰辅酶A。

    Organisms for the production of 1,3-butanediol
    270.
    发明授权
    Organisms for the production of 1,3-butanediol 有权
    用于生产1,3-丁二醇的生物体

    公开(公告)号：US09017983B2

    公开(公告)日：2015-04-28

    申请号：US12772114

    申请日：2010-04-30

    Applicant: Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

    Inventor： Anthony P. Burgard ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

    IPC: C12N15/52 ,  C12P7/18 ,  C12N9/04

    CPC classification number: C12P7/18 ,  C07C29/80 ,  C12N9/0006 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1096 ,  C12N9/88 ,  C12N15/52 ,  C12Y101/01 ,  C12Y101/01157 ,  C12Y102/01 ,  C12Y203/01 ,  C12Y206/01 ,  C12Y401/01 ,  C12Y402/01 ,  C12Y403/01

    Abstract: A non-naturally occurring microbial organism includes a microbial organism having a 1,3-butanediol (1,3-BDO) pathway having at least one exogenous nucleic acid encoding a 1,3-BDO pathway enzyme expressed in a sufficient amount to produce 1,3-BDO. The pathway includes an enzyme selected from a 2-amino-4-ketopentanoate (AKP) thiolase, an AKP dehydrogenase, a 2-amino-4-hydroxypentanoate aminotransferase, a 2-amino-4-hydroxypentanoate oxidoreductase (deaminating), a 2-oxo-4-hydroxypentanoate decarboxylase, a 3-hydroxybutyraldehyde reductase, an AKP aminotransferase, an AKP oxidoreductase (deaminating), a 2,4-dioxopentanoate decarboxylase, a 3-oxobutyraldehyde reductase (ketone reducing), a 3-oxobutyraldehyde reductase (aldehyde reducing), a 4-hydroxy-2-butanone reductase, an AKP decarboxylase, a 4-aminobutan-2-one aminotransferase, a 4-aminobutan-2-one oxidoreductase (deaminating), a 4-aminobutan-2-one ammonia-lyase, a butenone hydratase, an AKP ammonia-lyase, an acetylacrylate decarboxylase, an acetoacetyl-CoA reductase (CoA-dependent, aldehyde forming), an acetoacetyl-CoA reductase (CoA-dependent, alcohol forming), an acetoacetyl-CoA reductase (ketone reducing), a 3-hydroxybutyryl-CoA reductase (aldehyde forming), a 3-hydroxybutyryl-CoA reductase (alcohol forming), a 4-hydroxybutyryl-CoA dehydratase, and a crotonase. A method for producing 1,3-BDO, includes culturing such microbial organisms under conditions and for a sufficient period of time to produce 1,3-BDO.

    Abstract translation: 非天然存在的微生物生物体包括具有1,3-丁二醇（1,3-BDO）途径的微生物生物，其具有至少一种编码1,3-BDO途径酶的外源核酸，所述外源核酸以足够的量表达，以产生1 ，3-BDO。 该途径包括选自2-氨基-4-酮戊酸酯（AKP）硫解酶，AKP脱氢酶，2-氨基-4-羟基戊酸转氨酶，2-氨基-4-羟基戊酸酯氧化还原酶（脱氨基），2- 4-羟基戊酸脱羧酶，3-羟基丁醛还原酶，AKP氨基转移酶，AKP氧化还原酶（脱氨），2,4-二氧代戊酸脱羧酶，3-氧代丁醛还原酶（还原酮），3-氧代丁醛还原酶 ），4-羟基-2-丁酮还原酶，AKP脱羧酶，4-氨基丁-2-酮氨基转移酶，4-氨基丁-2-酮氧化还原酶（脱氨基），4-氨基丁-2-酮氨裂解酶 乙酰乙酰辅酶A还原酶（CoA依赖性醛形成），乙酰乙酰辅酶A还原酶（CoA依赖性醇形成），乙酰乙酰辅酶A还原酶（酮），乙酰乙酰辅酶A还原酶 还原），3-羟基丁酰辅酶A还原酶（醛形成），3-羟基 Ryl-CoA还原酶（醇形成），4-羟基丁酰辅酶A脱水酶和巴豆酸酶。 一种生产1,3-BDO的方法包括在条件和足够的时间内培养这些微生物生产1,3-BDO。

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