公开(公告)号：US20020198144A1

公开(公告)日：2002-12-26

申请号：US09900379

申请日：2001-07-06

申请人： Dade International, Inc.

发明人： Hing C. Wong ,  Peter R. Rhode ,  Jon A. Weidanz ,  Susan Grammer ,  Ana C. Edwards ,  Pierre-Andre Chavaillaz ,  Jin-An J.J. Jiao

IPC分类号： A61K038/17 ,  C07K014/74 ,  A61K039/395

CPC分类号： C07K14/705 ,  A61K38/00 ,  A61K39/00 ,  A61K48/00 ,  C07K14/70532 ,  C07K14/70539 ,  C07K14/77 ,  C07K2319/00 ,  C12N2799/026 ,  Y10S530/868

摘要： The present invention relates to novel complexes of major histocompability complex (MHC) molecules and uses of such complexes. In particular, the invention relates to MHC fusion complexes that contain a MHC molecule with a peptide-binding groove and a presenting peptide covalently linked to the MHC protein. Fusion complexes of the invention are useful for a variety of applications including in vitro screens for identification and isolation of peptides that modulate activity of selected T cells, including peptides that are T cell receptor antagonists and partial agonists, methods of suppressing an immune response of a mammal and methods for inducing an immune response in a mammal.

摘要翻译： 本发明涉及主要组氨酸复合物（MHC）分子的新型复合物和这种复合物的用途。 特别地，本发明涉及MHC融合复合物，其含有具有肽结合槽的MHC分子和与MHC蛋白共价连接的呈递肽。 本发明的融合复合物可用于各种应用，包括用于鉴定和分离调节所选T细胞活性的肽的体外筛选，包括作为T细胞受体拮抗剂和部分激动剂的肽，抑制α受体拮抗剂的免疫应答的方法 哺乳动物和哺乳动物诱导免疫应答的方法。

公开(公告)号：US06013531A

公开(公告)日：2000-01-11

申请号：US518135

申请日：1995-08-22

申请人： Chaeo-Huej J. Wang ,  Dinesh O. Shah

发明人： Chaeo-Huej J. Wang ,  Dinesh O. Shah

IPC分类号： C08F257/02 ,  C08F285/00 ,  C12Q1/68 ,  G01N33/543 ,  G01N33/58 ,  G01N33/553 ,  G01N33/546

CPC分类号： C12Q1/6834 ,  C08F257/02 ,  C08F285/00 ,  G01N33/54326 ,  G01N33/582 ,  G01N33/585 ,  G01N2446/10 ,  G01N2446/40 ,  G01N2446/84 ,  G01N2446/86 ,  Y10S435/967

摘要： This invention provides a novel process of using magnetically responsive fluorescent polymer particles comprising polymeric core particles coated evenly with a layer of polymer containing magnetically responsive metal oxide as highly sensitive quantitative reagents for biochemical and immunological studies, wherein the fluorescent magnetic particles can serve as a marker for the number of particles. A wide variety of polymeric particles with sizes ranging from 1 to 100 microns can be used a core particles and transformed into magnetically responsive polymer particles. The surface of these magnetically responsive polymer particles can be coated further with another layer of functionalized polymer. These magnetically responsive fluorescent polymer particles can be used for passive or covalent coupling of biological material such as antigens, antibodies, enzymes or DNA/RNA hybridization and used as solid phase for various types of immunoassays, DNA/RNA hybridization probes assays, affinity purification, cell separation and other medical, diagnostic, and industrial applications.

摘要翻译： 本发明提供了一种使用磁响应荧光聚合物颗粒的新方法，其包括均匀包被含有磁响应金属氧化物的聚合物层的聚合物芯颗粒，作为生物化学和免疫学研究的高灵敏度定量试剂，其中荧光磁性颗粒可以作为标记 对于颗粒数量。 尺寸范围为1至100微米的多种聚合物颗粒可用于核心颗粒并转化为磁响应聚合物颗粒。 这些磁响应聚合物颗粒的表面可以进一步被另一层官能化聚合物涂覆。 这些磁响应荧光聚合物颗粒可用于生物材料如抗原，抗体，酶或DNA / RNA杂交的被动或共价偶联，并用作各种类型的免疫测定的固相，DNA / RNA杂交探针测定，亲和纯化， 细胞分离等医疗，诊断和工业应用。

公开(公告)号：US5776784A

公开(公告)日：1998-07-07

申请号：US585333

申请日：1996-01-11

申请人： Joseph Edward Kegelman ,  Diane Kathleen Stille ,  Robert Kyle Wiedenmann ,  Paul John Zuk

发明人： Joseph Edward Kegelman ,  Diane Kathleen Stille ,  Robert Kyle Wiedenmann ,  Paul John Zuk

IPC分类号： G01N33/543 ,  B03C1/28 ,  G01N1/10 ,  G01N33/553 ,  G01N35/00 ,  G01N35/02 ,  G01N35/04 ,  G01N35/10

CPC分类号： B03C1/288 ,  G01N2001/1006 ,  G01N2035/0436 ,  G01N2035/1088 ,  G01N35/0098 ,  Y10S436/815

摘要： Assays for separating magnetic particles include separating magnetically responsive particles from a liquid dispersion disposed in a plurality of reaction vessels, and transporting the reaction vessels in sequence past at least one processing position. A robotics reagent arm and probe dispense reagents into the reaction vessels and a reaction monitoring device is capable of relative movement with respect to the transporting device. Incomplete separation is effected by positioning a magnetic field in contact with the reaction vessel for a first shortened time interval during which the particles partially aggregate and afterwards are removed from the reaction vessel. The magnet is repositioned in contact with the reaction vessel for a third time interval to achieve full separation of particles from the liquid.

摘要翻译： 用于分离磁性颗粒的测定包括从设置在多个反应容器中的液体分散体分离磁性响应性颗粒，并且将反应容器顺序地输送至至少一个处理位置。 机器人试剂臂和探针将试剂分配到反应容器中，反应监测装置能够相对于输送装置相对运动。 不完全的分离是通过将磁场定位成与反应容器接触的第一缩短的时间间隔来进行的，在此间隔期间颗粒部分聚集，之后从反应容器中移除。 将磁体重新定位成与反应容器接触第三时间间隔以实现颗粒与液体的完全分离。

公开(公告)号：US5770390A

公开(公告)日：1998-06-23

申请号：US621050

申请日：1996-03-22

申请人： Wilhelm Stoffel

发明人： Wilhelm Stoffel

IPC分类号： G01N33/531 ,  G01N33/543 ,  G01N33/92 ,  G01N33/545

CPC分类号： G01N33/54306 ,  G01N33/531 ,  G01N33/92 ,  Y10S436/822

摘要： Lipohaptens of the general formula I (X).sub.m --R--Y--(S).sub.n --P (I) wherein R is a hydrocarbyl backbone having from 2 to 30 carbon atoms X is a hydrocarbyl radical having from 6 to 30 carbon atoms, which is linked to R via a bonding group containing one or more heteroatoms m is an integer from 1 to 5 P is a polypeptide or polysaccharide residue having from 2 to 100 aminoacid or glycosyl units Y is a functional group capable of binding to a free amino group of the polypeptide P or a carboxy, hemiacetal or hydroxy group of the polysaccharide P S is a difunctional spacer group, the functional groups thereof being capable of binding with the functional group Y on the one hand and a free amino, carboxy or hydroxy group of the polypeptide or polysaccaride P on the other hand, and n is 0 or 1 and their use in immunoreactions for determining antibodies and antigens, respectively.

摘要翻译： 通式I（X）mry-（S）nP（I）的通式（I）的巯基联苯，其中R是具有2-30个碳原子的烃基主链X是具有6至30个碳原子的烃基，其与R通 含有一个或多个杂原子的键合基团是1至5的整数.P是具有2至100个氨基酸或糖基单元的多肽或多糖残基Y是能够结合多肽P的游离氨基的官能团或 多糖PS的羧基，半缩醛或羟基是双官能间隔基团，其功能基团一方面可以与官能团Y和多肽或多糖的P的游离氨基，羧基或羟基结合 另一方面，n为0或1，它们分别用于免疫反应以确定抗体和抗原。

公开(公告)号：US5763284A

公开(公告)日：1998-06-09

申请号：US428733

申请日：1995-04-25

申请人： Rony Tal ,  Hing C. Wong ,  Clayton Casipit ,  Pierre-Andre Chavaillaz ,  Vaughan Wittman

发明人： Rony Tal ,  Hing C. Wong ,  Clayton Casipit ,  Pierre-Andre Chavaillaz ,  Vaughan Wittman

IPC分类号： G01N33/53 ,  A61K38/00 ,  A61K39/00 ,  A61P37/00 ,  A61P37/04 ,  C07K1/30 ,  C07K14/32 ,  C07K14/465 ,  C07K14/47 ,  C12N15/09 ,  C12N15/62 ,  C12P21/02 ,  C12R1/10 ,  C12R1/19 ,  G01N33/531 ,  G01N33/536 ,  G01N33/68

CPC分类号： C07K14/32 ,  C07K14/465 ,  C07K14/4706 ,  C07K14/4716 ,  C12N15/62 ,  C07K2319/50 ,  C07K2319/72 ,  C07K2319/80

摘要： The present invention provides novel methods for the synthesis and isolation and purification of a peptide of interest (target peptide). In particular, the invention relates to peptide synthesis, isolation and purification methods that comprise use of penI fusion polypeptides and related gene fusion constructs that encode such polypeptides.

摘要翻译： 本发明提供用于合成和分离和纯化目标肽（靶肽）的新方法。 特别地，本发明涉及肽合成，分离和纯化方法，其包括使用penI融合多肽和编码这种多肽的相关基因融合构建体。

公开(公告)号：US5747268A

公开(公告)日：1998-05-05

申请号：US351869

申请日：1994-12-08

申请人： Kathryn Herring ,  Denise Sandberg

发明人： Kathryn Herring ,  Denise Sandberg

IPC分类号： G01N33/50 ,  G01N33/574 ,  G01N33/96 ,  G01N33/53

CPC分类号： G01N33/574 ,  G01N33/57488 ,  G01N33/96 ,  G01N2333/695 ,  G01N2333/705 ,  G01N2333/82 ,  Y10S435/967 ,  Y10S436/813

摘要： The present invention provides a stable control serum or plasma for tumor diagnosis, wherein the control contains the tumor markers relevant for the diagnosis of tumors. The serum or plasma has a reduced lipid content. The present invention also provides for a method of making the control.

摘要翻译： 本发明提供用于肿瘤诊断的稳定的对照血清或血浆，其中所述对照含有与肿瘤诊断相关的肿瘤标志物。 血清或血浆脂质含量降低。 本发明还提供了一种进行控制的方法。

公开(公告)号：US5717148A

公开(公告)日：1998-02-10

申请号：US592774

申请日：1996-01-26

申请人： Keith Aldan Ely ,  Timothy Patrick Evers ,  Robert Eric Bernstine

发明人： Keith Aldan Ely ,  Timothy Patrick Evers ,  Robert Eric Bernstine

IPC分类号： G01N35/02 ,  G01N30/80 ,  G01N35/04 ,  G01N35/10 ,  G01N35/00

CPC分类号： G01N35/1083 ,  G01N2035/0441 ,  G01N2035/1076 ,  G01N2035/1086 ,  G01N35/025 ,  G01N35/1004

摘要： An analysis instrument includes a container support (12), a carrier (24) having a plurality of liquid receiving wells (24W), a gross probe (40) movable along a locus of action (42) and a fine probe (50), the fine probe (50) being movable along a locus of action (52). In addition, various analysis, treatment and/or handling devices are also disposed at predetermined operating positions arranged along the loci of action (42, 52). Both the gross probe 40 and the fine probe 50 are each independently movable along their respective loci of action 42, 52 among one or more various extracting position(s) and one or more various dispensing position(s) to which either the support (12) and/or the carrier (24) may be moved, and/or among various operating position(s).

摘要翻译： 分析仪器包括容器支架（12），具有多个液体接收孔（24W）的载体（24），沿着动作轨迹（42）可移动的总探头（40）和细探针（50） 所述精细探针（50）可沿着动作轨迹（52）移动。 此外，各种分析，处理和/或处理装置也被布置在沿着动作轨迹（42,42）布置的预定操作位置。 总探针40和细探针50都可以沿着它们各自的一个或多个提取位置和一个或多个各种分配位置中的各自的作用位置52,52彼此独立地移动，支撑体（12） ）和/或载体（24）可以移动和/或在各种操作位置之间。

公开(公告)号：US5688944A

公开(公告)日：1997-11-18

申请号：US473810

申请日：1995-06-07

申请人： Chengrong Wang

发明人： Chengrong Wang

IPC分类号： G01N33/53 ,  A61K39/00 ,  C07D223/22 ,  C07D223/26 ,  C07D223/32 ,  G01N33/532 ,  G01N33/94 ,  G01N33/546

CPC分类号： G01N33/532 ,  C07D223/22 ,  C07D223/32 ,  G01N33/9473 ,  G01N33/9486 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S435/975 ,  Y10S436/823 ,  Y10S530/806 ,  Y10T436/171538

摘要： The present invention provides a novel carbamazepine hydrazide compound suitable for covalent attachment to a polymer particle reagent, having the general formula ##STR1## The present invention also provides a novel carbamazepine acid compound suitable for attachment to proteins for the production of carbamazepine immunogens. The carbamazepine antigen of the present invention has the following general structure: ##STR2## where X is C.dbd.O, CH.sub.2 or SO.sub.2 ; Y is C.dbd.O, CH.sub.2, SO.sub.2 ; R is an alkyl and P is a protein or a hapten.

摘要翻译： 本发明提供了一种新颖的卡马西平酰肼化合物，其适用于共价连接到具有通式的聚合物颗粒试剂。本发明还提供了一种新颖的卡马西平酸化合物，其适于与用于生产卡马西平免疫原的蛋白质连接。 本发明的卡马西平抗原具有以下通用结构：其中X为C = O，CH 2或SO 2; Y为C = O，CH 2，SO 2; R是烷基，P是蛋白质或半抗原。

公开(公告)号：US5627038A

公开(公告)日：1997-05-06

申请号：US322970

申请日：1994-10-13

申请人： H. C. Hemker

发明人： H. C. Hemker

IPC分类号： C12Q1/37 ,  C12Q1/56 ,  G01N33/53

CPC分类号： C12Q1/56 ,  C12Q2337/12 ,  G01N2333/96444 ,  G01N2333/9645 ,  G01N2333/96452 ,  Y10S435/966 ,  Y10S435/975 ,  Y10S436/808

摘要： A chromogenic assay for determination of blood coagulation Factor IX (christmas factor) utilizes Factor Xa formed by the conversion of Factor X by activated Factor IX and Factor VIII to cleave a chromogenic substrate. The sample is combined into a mixture with Factor XIa in the presence of Factor IIa (thrombin), incubated, combined with Factor X and Factor VIII, and incubated. A thrombin inhibitor, and an indicator agent which reacts with Factor Xa, are added and the resulting signal measured and correlated to the level of Factor IX in the sample.

摘要翻译： 用于测定血液凝固因子IX（圣诞节因子）的显色测定利用通过活化因子IX和因子VIII转化因子X形成的因子Xa，以切割显色底物。 在因子IIa（凝血酶）存在下将样品与因子XIa混合，孵育，与因子X和因子VIII组合，并孵育。 加入凝血酶抑制剂和与因子Xa反应的指示剂，并测量所得信号并与样品中因子IX的水平相关。

公开(公告)号：US5526360A

公开(公告)日：1996-06-11

申请号：US375543

申请日：1995-01-25

申请人： Clifford H. Kraft

发明人： Clifford H. Kraft

IPC分类号： H04J3/00 ,  H04J3/04

CPC分类号： H04J3/047

摘要： A high-speed burst digital time multiplexed data system has N parallel input data paths that are multiplexed onto a serial data path for transmission from a transmitter to a receiver. Serial transmission takes place in a short burst upon command at the transmitter. Data from the serial data path is demultiplexed back into N parallel data paths at the receiver. The entire process is accomplished asynchronously without the aid of a clock or framing signal. In the preferred embodiment, a train of N sampling pulses is generated by two tapped delay lines, one at the transmitter and one at the receiver. The length of each sequential sampling pulse is determined by the tap spacing of the delay line, and the duration of the entire burst process is equal to the total delay of the delay line. A new burst may be initiated at any time after the completion of the previous burst. Thus bursts may follow each other immediately or be arbitrarily spaced to occur whenever data transmission is required.

摘要翻译： 高速脉冲串数字时间复用数据系统具有N个并行输入数据路径，其被复用到串行数据路径上，用于从发射机到接收机的传输。 串行传输在发射机的命令中以短脉冲串发生。 来自串行数据路径的数据在接收机被解复用为N个并行数据路径。 整个过程是不经过时钟或帧信号的异步完成的。 在优选实施例中，通过两个抽头延迟线产生一列N个采样脉冲，一个在发射机处，一个在接收机处。 每个顺序采样脉冲的长度由延迟线的抽头间距确定，并且整个突发处理的持续时间等于延迟线的总延迟。 可以在完成先前的突发之后的任何时间启动新的突发。 因此，当需要数据传输时，突发可以立即彼此跟随或任意间隔。