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    Device and system for creating and maintaining a localized environment for a cell culture plate
    22.
    发明授权
    Device and system for creating and maintaining a localized environment for a cell culture plate 有权

    公开(公告)号：US10351812B2

    公开(公告)日：2019-07-16

    申请号：US14838575

    申请日：2015-08-28

    Applicant: Axion BioSystems, Inc.

    Inventor： Robert Dixon Grier, Jr. ,  David Ernest Irvine ,  James David Ross

    IPC: C12M1/00 ,  C12M1/12 ,  C12M1/32 ,  C12M1/34 ,  C12M3/00

    Abstract: Systems and methods for creating a localized environment for a cell culture plate are described herein. An example system can include a dock shaped to receive the cell culture plate, and a gas distribution device positioned on the dock. The gas distribution device can include a frame including a plurality of sides connected to form an opening, an internal channel within the frame for directing a gas mixture within the gas distribution device, an inlet port in fluid communication with the internal channel for receiving the gas mixture, and a plurality of apertures in fluid communication with the internal channel. The plurality of apertures can be spaced apart from one another along the frame and configured to direct the gas mixture toward the opening.

    SYSTEMS AND METHODS FOR ASSESSING DATA COLLECTED FROM AN ELECTRICALLY ACTIVE CELL CULTURE
    25.
    发明申请
    SYSTEMS AND METHODS FOR ASSESSING DATA COLLECTED FROM AN ELECTRICALLY ACTIVE CELL CULTURE 有权
    用于评估从电动活性细胞培养物收集的数据的系统和方法

    公开(公告)号：US20160274088A1

    公开(公告)日：2016-09-22

    申请号：US15076223

    申请日：2016-03-21

    Applicant: Axion BioSystems, Inc.

    Inventor： Stacie Ann Chvatal ,  Daniel Christopher Millard ,  James David Ross

    IPC: G01N33/50 ,  H04L29/08

    CPC classification number: G01N33/5044 ,  A61B5/04 ,  A61B5/0464 ,  G01N33/5058 ,  G01N33/5061 ,  G01N33/5091 ,  G01N2800/326 ,  H04L67/12

    Abstract: Disclosed herein are systems and methods for assessing electrically active cell cultures. Optionally, the data can be collected using a microelectrode array (MEA). For example, electrically active cells, such as cardiomyocytes, are cultured such that they are in electrical communication with at least a portion of the electrodes of a well of the MEA. The assessments derived from the disclosed methods may be used to reduce the effects of confounding variables in data obtained from an electrically active cell culture. The methods may also be used to determine a quantitative measure of arrhythmia burden. The methods may also be used to decide if a particular culture or set of data is suitable for inclusion in scientific and characterization studies. Also disclosed is a method of finding the global conduction velocity of an electrically active cell culture.

    Abstract translation: 本文公开了用于评估电活性细胞培养物的系统和方法。 任选地，可以使用微电极阵列（MEA）收集数据。 例如，培养电活性细胞，例如心肌细胞，使得它们与MEA的孔的至少一部分电极电连通。 从公开的方法得到的评估可用于减少混合变量对从电活性细胞培养物获得的数据的影响。 方法也可用于确定心律失常负荷的定量测量。 这些方法也可用于确定特定文化或数据集是否适合纳入科学和表征研究。 还公开了一种找到电活性细胞培养物的全局传导速度的方法。

    Devices, systems and methods for high-throughput electrophysiology
    26.
    发明授权
    Devices, systems and methods for high-throughput electrophysiology 有权
    用于高通量电生理学的装置，系统和方法

    公开(公告)号：US09329168B2

    公开(公告)日：2016-05-03

    申请号：US14744513

    申请日：2015-06-19

    Applicant: AXION BIOSYSTEMS, INC.

    Inventor： Swaminathan Rajaraman ,  James D Ross ,  Amanda Preyer

    IPC: G01N27/327 ,  G01N33/50 ,  G01N33/483

    CPC classification number: G01N33/5014 ,  B01L3/5085 ,  B01L2200/025 ,  B01L2300/0645 ,  B01L2300/0829 ,  C12M23/12 ,  G01N33/4836

    Abstract: Electrophysiology culture plates are provided and are formed from a transparent microelectrode array (MEA) plate. The MEA plate comprises a substrate, a first layer and a first insulating layer. The substrate has a plurality of vias extending from an upper to a lower surface, each via being in electrical contact with each of a plurality of contact pads disposed on the lower surface. The first layer is disposed on the upper surface of the substrate and has a plurality of MEA arrays in electrical communication with at least a first routing layer. Each MEA array comprises a plurality of reference electrodes and a plurality of microelectrodes and the first routing layer is in electrical communication with a select number of the plurality of vias. A first insulating layer is disposed on the first layer. The MEA plate is joined to a biologic culture plate having a plurality of culture wells such that each culture well defines an interior cavity having a bottom surface that is at least partially transparent and in positioned in registration with a select optical port. The MEA plate is coupled to the biologic culture well plate such that each MEA array is operatively coupled to one culture well wherein each microelectrode and each reference electrode are in electrical communication with the interior cavity through the bottom surface of the culture well.

    Abstract translation: 提供电生理培养板并由透明微电极阵列（MEA）板形成。 MEA板包括基底，第一层和第一绝缘层。 衬底具有从上表面延伸到下表面的多个通孔，每个通孔与设置在下表面上的多个接触焊盘中的每一个电接触。 第一层设置在衬底的上表面上并且具有与至少第一路由层电连通的多个MEA阵列。 每个MEA阵列包括多个参考电极和多个微电极，并且第一路由层与选择数量的多个通孔电连通。 第一绝缘层设置在第一层上。 将MEA板连接到具有多个培养孔的生物培养板，使得每个培养井定义具有至少部分透明的底表面并定位成与选择光学端口对准的内部空腔。 将MEA板耦合到生物培养孔板，使得每个MEA阵列可操作地偶联到一个培养孔，其中每个微电极和每个参比电极通过培养孔的底表面与内腔电连通。

    Devices, systems and methods for high-throughput electrophysiology
    27.
    发明授权
    Devices, systems and methods for high-throughput electrophysiology 有权
    用于高通量电生理学的装置，系统和方法

    公开(公告)号：US09279801B2

    公开(公告)日：2016-03-08

    申请号：US14279961

    申请日：2014-05-16

    Applicant: AXION BIOSYSTEMS, INC.

    Inventor： Swaminathan Rajaraman ,  James D. Ross ,  Amanda Preyer

    IPC: G01N27/327 ,  G01N33/50 ,  G01N33/483

    CPC classification number: G01N33/5014 ,  B01L3/5085 ,  B01L2200/025 ,  B01L2300/0645 ,  B01L2300/0829 ,  C12M23/12 ,  G01N33/4836

    Abstract: Electrophysiology culture plates are provided and are formed from a transparent microelectrode array (MEA) plate. The MEA plate comprises a substrate, a first layer and a first insulating layer. The substrate has a plurality of vias extending from an upper to a lower surface, each via being in electrical contact with each of a plurality of contact pads disposed on the lower surface. The first layer is disposed on the upper surface of the substrate and has a plurality of MEA arrays in in electrical communication with at least a first routing layer. Each MEA array comprises a plurality of reference electrodes and a plurality of microelectrodes and the first routing layer is in electrical communication with a select number of the plurality of vias. A first insulating layer is disposed on the first layer. The MEA plate is joined to a biologic culture plate having a plurality of culture wells such that each culture well defines an interior cavity having a bottom surface that is at least partially transparent and in positioned in registration with a select optical port. The MEA plate is coupled to the biologic culture well plate such that each MEA array is operatively coupled to one culture well wherein each microelectrode and each reference electrode are in electrical communication with the interior cavity through the bottom surface of the culture well.

    Abstract translation: 提供电生理培养板并由透明微电极阵列（MEA）板形成。 MEA板包括基底，第一层和第一绝缘层。 衬底具有从上表面延伸到下表面的多个通孔，每个通孔与设置在下表面上的多个接触焊盘中的每一个电接触。 第一层设置在衬底的上表面上并且具有与至少第一路由层电通信的多个MEA阵列。 每个MEA阵列包括多个参考电极和多个微电极，并且第一路由层与选择数量的多个通孔电连通。 第一绝缘层设置在第一层上。 将MEA板连接到具有多个培养孔的生物培养板，使得每个培养井定义具有至少部分透明的底表面并定位成与选择光学端口对准的内部空腔。 将MEA板耦合到生物培养孔板，使得每个MEA阵列可操作地偶联到一个培养孔，其中每个微电极和每个参比电极通过培养孔的底表面与内腔电连通。

    3D MICROELECTRODE DEVICE FOR LIVE TISSUE APPLICATIONS
    29.
    发明申请
    3D MICROELECTRODE DEVICE FOR LIVE TISSUE APPLICATIONS 有权
    用于实际组织应用的3D微电子设备

    公开(公告)号：US20130338746A1

    公开(公告)日：2013-12-19

    申请号：US13919013

    申请日：2013-06-17

    Applicant: Georgia Institute of Technology ,  Axion Biosystems, Inc.

    Inventor： GARETH S. GUVANASEN ,  SWAMINATHAN RAJARAMAN ,  RICARDO AGUILAR, JR. ,  LIANG GUO ,  T. RICHARD NICHOLS ,  STEPHEN P. DEWEERTH

    IPC: A61N1/05

    CPC classification number: A61N1/0502 ,  A61B5/04001 ,  A61B5/0492 ,  A61B2562/0209 ,  A61B2562/125 ,  A61B2562/164 ,  A61N1/0551 ,  H05K1/0283 ,  H05K1/095 ,  H05K2201/0245 ,  H05K2201/10318

    Abstract: A 3D microelectrode device includes a flexible substrate containing poly-dimethyl siloxane (PDMS). The device may be fabricated in a miniature form factor suitable for attachment to a small organ such as a lateral gastrocnemius muscle of a live rat. In addition to providing a miniaturized, conformable attachment, the device provides an anchoring action via one or more microelectrodes, each having an insertable tip particularly shaped to provide the anchoring action. Furthermore, a base portion of each of the microelectrodes is embedded inside conductive poly-dimethyl siloxane (cPDMS). The cPDMS is contained in a pad that is coupled to a conductive track embedded in the flexible substrate. Embedding of the base portion inside the cPDMS material not only allows the microelectrode to bend in various directions, but also provides good electrical conductivity while eliminating the need for attachment processes using solder or epoxy adhesives.

    Abstract translation: 3D微电极装置包括含有聚二甲基硅氧烷（PDMS）的柔性基材。 该装置可以以适于附着到活体大鼠的腓肠肌外侧的小器官的微型形状来制造。 除了提供小型化，顺应性附件之外，该装置通过一个或多个微电极提供锚定作用，每个微电极具有特别成形以提供锚定作用的可插入尖端。 此外，每个微电极的基底部分嵌入导电性聚二甲基硅氧烷（cPDMS）内部。 cPDMS包含在耦合到嵌入在柔性基板中的导电轨道的焊盘中。 在cPDMS材料内部嵌入基底部分不仅允许微电极在各个方向上弯曲，而且还提供良好的导电性，同时不需要使用焊料或环氧树脂粘合剂的连接工艺。

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