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公开(公告)号:US10821439B2
公开(公告)日:2020-11-03
申请号:US15659855
申请日:2017-07-26
Applicant: Berkeley Lights, Inc.
Inventor: Keith J. Breinlinger , Eric D. Hobbs , Daniele Malleo , J. Tanner Nevill , Mark P. White
Abstract: A microfluidic apparatus is provided having one or more sequestration pens configured to isolate one or more target micro-objects by changing the orientation of the microfluidic apparatus with respect to a globally active force, such as gravity. Methods of selectively directing the movements of micro-objects in such a microfluidic apparatus using gravitational forces are also provided. The micro-objects can be biological micro-objects, such as cells, or inanimate micro-objects, such as beads.
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公开(公告)号:US20200078785A1
公开(公告)日:2020-03-12
申请号:US16509414
申请日:2019-07-11
Applicant: Berkeley Lights, Inc.
Inventor: Eric D. Hobbs , Justin K. Valley
IPC: B01L3/00 , H01L27/146 , B03C5/02 , B03C5/00
Abstract: A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.
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公开(公告)号:US10350594B2
公开(公告)日:2019-07-16
申请号:US15912164
申请日:2018-03-05
Applicant: Berkeley Lights, Inc.
Inventor: Eric D. Hobbs , Justin K. Valley
IPC: G01N1/10 , G01N1/00 , G01N35/00 , G01N33/48 , B01L3/00 , B03C5/00 , B03C5/02 , H01L27/146 , H01L29/732
Abstract: A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.
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24.
公开(公告)号:US20190134630A1
公开(公告)日:2019-05-09
申请号:US15989549
申请日:2018-05-25
Applicant: Berkeley Lights, Inc.
Inventor: Mark P. White , Eric D. Hobbs , J. Tanner Nevill , Daniele Malleo , Steven W. Short
IPC: B01L3/00 , G01N33/558
Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
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25.
公开(公告)号:US20180298318A1
公开(公告)日:2018-10-18
申请号:US16010176
申请日:2018-06-15
Applicant: Berkeley Lights, Inc.
Inventor: Volker L.S. Kurz , Troy A. Lionberger , Erik K. Sackmann , Kai W. Szeto , Paul M. Lebel , Brandon R. Bruhn , Keith J. Breinlinger , Eric D. Hobbs , Andrew W. McFarland , J. Tanner Nevill , Xiaohua Wang
CPC classification number: C12M23/16 , B01L3/502761 , B01L2200/0668 , B01L2300/1822 , B01L2400/0415 , B01L2400/0427 , B01L2400/0442 , B01L2400/0454 , B01L2400/086 , C12M23/20 , C12M33/12
Abstract: Apparatuses and methods are described for the use of optically driven bubble, convective and displacing fluidic flow to provide motive force in microfluidic devices. Alternative motive modalities are useful to selectively dislodge and displace micro-objects, including biological cells, from a variety of locations within the enclosure of a microfluidic device.
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公开(公告)号:US20180193835A1
公开(公告)日:2018-07-12
申请号:US15912164
申请日:2018-03-05
Applicant: Berkeley Lights, Inc.
Inventor: Eric D. Hobbs , Justin K. Valley
IPC: B01L3/00 , B03C5/00 , B03C5/02 , H01L27/146 , H01L29/732
CPC classification number: B01L3/502715 , B01L3/502707 , B01L3/50273 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0819 , B01L2400/0415 , B01L2400/0424 , B03C5/005 , B03C5/026 , H01L27/14681 , H01L29/732
Abstract: A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.
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27.
公开(公告)号:US10010882B2
公开(公告)日:2018-07-03
申请号:US14520568
申请日:2014-10-22
Applicant: Berkeley Lights, Inc.
Inventor: Mark P. White , Eric D. Hobbs , J. Tanner Nevill , Daniele Malleo , Steven W. Short
IPC: B01L3/00 , G01N33/558
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0424 , B01L2400/0433 , B01L2400/0454 , G01N33/558 , G01N2469/20
Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
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公开(公告)号:US09908115B2
公开(公告)日:2018-03-06
申请号:US14961215
申请日:2015-12-07
Applicant: Berkeley Lights, Inc.
Inventor: Eric D. Hobbs , Justin K. Valley
IPC: G01N15/06 , B01L3/00 , H01L21/00 , G01N25/18 , B03C5/00 , B03C5/02 , H01L27/146 , H01L29/732
CPC classification number: B01L3/502715 , B01L3/502707 , B01L3/50273 , B01L3/502761 , B01L2200/0647 , B01L2300/0816 , B01L2300/0819 , B01L2400/0415 , B01L2400/0424 , B03C5/005 , B03C5/026 , H01L27/14681 , H01L29/732
Abstract: A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.
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29.
公开(公告)号:US20160338347A1
公开(公告)日:2016-11-24
申请号:US15136777
申请日:2016-04-22
Applicant: BERKELEY LIGHTS, INC.
Inventor: Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, JR.
CPC classification number: A01N1/0284 , A01N1/0221 , A01N1/0263 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2300/021 , B01L2300/024 , B01L2300/0864 , B01L2300/16 , B01L2300/163 , B01L2300/18 , B01L2300/1894 , C12M47/04
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
Abstract translation: 一种处理和存储生物细胞的方法包括将可流动介质引入微流体装置,所述可流动介质包括生物细胞; 在微流体装置的一个或多个隔离区域中从可流动介质中隔离一个或多个生物细胞; 以及冷冻微流体装置,其中包含一个或多个生物细胞。
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公开(公告)号:US11596941B2
公开(公告)日:2023-03-07
申请号:US17063704
申请日:2020-10-05
Applicant: Berkeley Lights, Inc.
Inventor: Eric D. Hobbs , Justin K. Valley
IPC: B01L3/00 , B03C5/00 , B03C5/02 , H01L27/146 , H01L29/732
Abstract: A microfluidic device can include a base an outer surface of which forms one or more enclosures for containing a fluidic medium. The base can include an array of individually controllable transistor structures each of which can comprise both a lateral transistor and a vertical transistor. The transistor structures can be light activated, and the lateral and vertical transistors can thus be photo transistors. Each transistor structure can be activated to create a temporary electrical connection from a region of the outer surface of the base (and thus fluidic medium in the enclosure) to a common electrical conductor. The temporary electrical connection can induce a localized electrokinetic force generally at the region, which can be sufficiently strong to move a nearby micro-object in the enclosure.
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