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公开(公告)号：US10800652B2
公开(公告)日：2020-10-13
申请号：US16123882
申请日：2018-09-06
Applicant: Berkeley Lights, Inc.
Inventor： Keith J. Breinlinger , Daniele Malleo , Gaetan L. Mathieu , J. Tanner Nevill , Alexander H. Slocum , Mark P. White
IPC: B81B7/02 , B03C5/00 , B03C5/02 , G01N27/447 , B03C7/02 , G01N33/543 , B01L3/00 , C12M3/06 , C12M1/00 , C12M1/34 , G01N33/50
Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.
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公开(公告)号：US20190152771A1
公开(公告)日：2019-05-23
申请号：US16123882
申请日：2018-09-06
Applicant: Berkeley Lights, Inc.
Inventor： Keith J. Breinlinger , Daniele Malleo , Gaetan L. Mathieu , J. Tanner Nevill , Alexander H. Slocum , Mark P. White
Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.
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23.发明申请公开(公告)号：US20190134630A1
公开(公告)日：2019-05-09
申请号：US15989549
申请日：2018-05-25
Applicant: Berkeley Lights, Inc.
Inventor： Mark P. White , Eric D. Hobbs , J. Tanner Nevill , Daniele Malleo , Steven W. Short
IPC: B01L3/00 , G01N33/558
Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
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公开(公告)号：US20190064038A1
公开(公告)日：2019-02-28
申请号：US16121398
申请日：2018-09-04
Applicant: Berkeley Lights, Inc.
Inventor： Mark P. White , Randall D. Lowe, Jr.
IPC: G01N1/34 , G01N27/447 , B01L3/00 , C12Q1/6806 , B03C5/02 , B03C5/00
Abstract: Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation region of a sequestration pen in a micro-fluidic device. The isolated nucleus/nuclei may then be retrieved from the isolation region of the sequestration pen and used in any desired downstream assay or process.
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25.发明授权公开(公告)号：US10010882B2
公开(公告)日：2018-07-03
申请号：US14520568
申请日：2014-10-22
Applicant: Berkeley Lights, Inc.
Inventor： Mark P. White , Eric D. Hobbs , J. Tanner Nevill , Daniele Malleo , Steven W. Short
IPC: B01L3/00 , G01N33/558
CPC classification number: B01L3/502761 , B01L2200/0647 , B01L2200/0652 , B01L2200/0668 , B01L2300/0816 , B01L2300/0864 , B01L2300/087 , B01L2400/0424 , B01L2400/0433 , B01L2400/0454 , G01N33/558 , G01N2469/20
Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
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Patent Agency Ranking
26.发明申请公开(公告)号：US20170276679A1
公开(公告)日：2017-09-28
申请号：US15406289
申请日：2017-01-13
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Mark P. White , Xiaohua Wang , Minha Park , Guido K. Stadler , Randall D. Lowe, Jr. , Xiao Guan , Jason M. McEwen , Gang Wang , George L. Fox , Peggy A. Radel
IPC: G01N33/574 , C12Q1/68 , G01N33/543 , B01L3/00
Abstract: A method of preparing an antibody therapeutic is provided comprising: (a) providing a dissociated cell sample from at least one solid tumor sample obtained from a patient; (b) loading the dissociated cell sample into a microfluidic device having a flow region and at least one isolation region fluidically connected to the flow region; (c) moving at least one B cell from the dissociated cell sample into at least one isolation region in the microfluidic device, thereby obtaining at least one isolated B cell; and (d) using the microfluidic device to identify at least one B cell that produces antibodies capable of binding to cancer cells. The cancer cells can be the patient's own cancer cells. Also provided are methods of treating patients, methods of labeling or detecting cancer, engineered T or NK cells comprising antibodies or fragments thereof, and engineered antibody constructs.
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公开(公告)号：US09617145B2
公开(公告)日：2017-04-11
申请号：US14520510
申请日：2014-10-22
Applicant: Berkeley Lights, Inc.
Inventor： Keith J. Breinlinger , Daniele Malleo , Gaetan L. Mathieu , J. Tanner Nevill , Alexander H. Slocum , Mark P. White
CPC classification number: B81B7/02 , B01L3/502761 , B01L2200/027 , B01L2200/0652 , B01L2200/0668 , B01L2300/044 , B01L2300/0636 , B01L2300/0645 , B01L2300/0816 , B01L2300/0819 , B01L2300/0829 , B01L2300/0851 , B01L2300/0864 , B01L2300/087 , B01L2300/0877 , B01L2400/0424 , B01L2400/0433 , B01L2400/0454 , B01L2400/086 , B03C5/005 , B03C5/026 , B03C7/023 , B03C2201/26 , C12M23/16 , C12M41/46 , C12M47/02 , G01N27/447 , G01N27/44756 , G01N27/44791 , G01N33/5023 , G01N33/543 , G01N33/54326 , G01N35/10
Abstract: A group of micro-objects in a holding pen in a micro-fluidic device can be selected and moved to a staging area, from which the micro-objects can be exported from the micro-fluidic device. The micro-fluidic device can have a plurality of holding pens, and each holding pen can isolate micro-objects located in the holding pen from micro-objects located in the other holding pens or elsewhere in the micro-fluidic device. The selected group of micro-objects can comprise one or more biological cells, such as a clonal population of cells. Embodiments of the invention can thus select a particular group of clonal cells in a micro-fluidic device, move the clonal cells to a staging area, and export the clonal cells from the micro-fluidic device while maintaining the clonal nature of the exported group.
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公开(公告)号：US20160338347A1
公开(公告)日：2016-11-24
申请号：US15136777
申请日：2016-04-22
Applicant: BERKELEY LIGHTS, INC.
Inventor： Mark P. White , Kevin T. Chapman , Andrew W. McFarland , Eric D. Hobbs , Randall D. Lowe, JR.
CPC classification number: A01N1/0284 , A01N1/0221 , A01N1/0263 , B01L3/502715 , B01L3/502761 , B01L2200/0668 , B01L2300/021 , B01L2300/024 , B01L2300/0864 , B01L2300/16 , B01L2300/163 , B01L2300/18 , B01L2300/1894 , C12M47/04
Abstract: A method of processing and storing biological cells includes introducing a flowable medium into a microfluidic device, the flowable medium including biological cells; sequestering one or more biological cells from the flowable medium in one or more isolation regions of the microfluidic device; and freezing the microfluidic device including the one or more biological cells sequestered therein.
Abstract translation: 一种处理和存储生物细胞的方法包括将可流动介质引入微流体装置，所述可流动介质包括生物细胞; 在微流体装置的一个或多个隔离区域中从可流动介质中隔离一个或多个生物细胞; 以及冷冻微流体装置，其中包含一个或多个生物细胞。
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29.发明授权公开(公告)号：US11565259B2
公开(公告)日：2023-01-31
申请号：US16683798
申请日：2019-11-14
Applicant: Berkeley Lights, Inc.
Inventor： Mark P. White , Eric D. Hobbs , J. Tanner Nevill , Daniele Malleo , Steven W. Short
IPC: B01L3/00 , G01N33/558
Abstract: A microfluidic device can comprise at least one swept region that is fluidically connected to unswept regions. The fluidic connections between the swept region and the unswept regions can enable diffusion but substantially no flow of media between the swept region and the unswept regions. The capability of biological micro-objects to produce an analyte of interest can be assayed in such a microfluidic device. Biological micro-objects in sample material loaded into a microfluidic device can be selected for particular characteristics and disposed into unswept regions. The sample material can then be flowed out of the swept region and an assay material flowed into the swept region. Flows of medium in the swept region do not substantially affect the biological micro-objects in the unswept regions, but any analyte of interest produced by a biological micro-object can diffuse from an unswept region into the swept region, where the analyte can react with the assay material to produce a localized detectable reaction. Any such detected reactions can be analyzed to determine which, if any, of the biological micro-objects are producers of the analyte of interest.
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公开(公告)号：US20220388003A1
公开(公告)日：2022-12-08
申请号：US17656244
申请日：2022-03-24
Applicant: Berkeley Lights, Inc.
Inventor： Kevin T. Chapman , Daniele Malleo , J. Tanner Nevill , Steven W. Short , Mark P. White , M. Jimena Loureiro
Abstract: Biological activity in holding pens in a micro-fluidic device can be assayed by placing in the holding pens capture objects that bind a particular material of interest produced by the biological activity. The biological material of interest that binds to each capture object can then be assessed, either in the micro-fluidic device or after exporting the capture object from the micro-fluidic device. The assessment can be utilized to characterize the biological activity in each holding pen. The biological activity can be production of the biological material of interest. Thus, the biological activity can correspond to or arise from one or more biological cells. Biological cells within a holding pen can be clonal cell colonies. The biological activity of each clonal cell colony can be assayed while maintaining the clonal status of each colony.
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