公开(公告)号：US20190040349A1

公开(公告)日：2019-02-07

申请号：US16152908

申请日：2018-10-05

申请人： EMULATE, Inc.

发明人： Daniel Levner ,  Josiah Daniel Sliz ,  Christopher David Hinojosa ,  Joshua Gomes ,  Jose Fernandez-Alcon

IPC分类号： C12M3/06 ,  A01N1/02 ,  C12M1/00 ,  B01L3/00 ,  C12M1/34 ,  C12M3/00 ,  C12M1/36 ,  C12M1/42 ,  C12N5/071

摘要： A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

公开(公告)号：US20190002811A1

公开(公告)日：2019-01-03

申请号：US16108827

申请日：2018-08-22

申请人： Emulate, Inc.

发明人： Daniel Levner ,  Josiah Daniel Sliz ,  Christopher David Hinojosa ,  Guy Robert Thompson, II ,  Petrus Wilhelmus Martinus van Ruijven ,  Matthew Daniel Solomon ,  Christian Alexander Potzner ,  Patrick Sean Tuohy

IPC分类号： C12M3/06 ,  C12N5/071 ,  C12M1/00 ,  B01L3/00 ,  B01L9/00 ,  C12M3/00 ,  C12M1/36 ,  C12M1/34 ,  C12M1/42 ,  A01N1/02

摘要： Drop-to-drop connection schemes are described for putting a microfluidic device in fluidic communication with a fluid source or another microfluidic device, including but not limited to, putting a microfluidic device in fluidic communication with the perfusion manifold assembly.

公开(公告)号：US20180305668A1

公开(公告)日：2018-10-25

申请号：US15958142

申请日：2018-04-20

申请人： EMULATE,INC. ,  CEDARS-SINAI MEDICAL CENTER

发明人： Dan Gazit ,  Gadi Pelled ,  Zulma Gazit ,  Dmitriy Sheyn ,  Christopher David Hinojosa ,  Norman Wen ,  Geraldine Hamilton

IPC分类号： C12N5/077 ,  C12N5/0775 ,  C12N5/00

CPC分类号： C12N5/0075 ,  A61K49/0019 ,  A61K49/005 ,  B01L3/5027 ,  C07K14/78 ,  C12N5/0062 ,  C12N5/0654 ,  C12N5/0663 ,  G01N33/5038

摘要： Microfluidic “organ-on-a-chip” devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.

公开(公告)号：US20180015465A1

公开(公告)日：2018-01-18

申请号：US15647831

申请日：2017-07-12

申请人： EMULATE, Inc.

发明人： Daniel Levner ,  Josiah Daniel Sliz ,  Christopher David Hinojosa ,  Joshua Gomes ,  Kyung Jin Jang

IPC分类号： B01L3/00

摘要： Methods of removing bubbles from a microfluidic device are described where the flow is not stopped. Methods are described that combine pressure and flow to remove bubbles from a microfluidic device. Bubbles can be removed even where the device is made of a polymer that is largely gas impermeable.

公开(公告)号：US12070746B2

公开(公告)日：2024-08-27

申请号：US15958142

申请日：2018-04-20

申请人： EMULATE,INC. ,  CEDARS-SINAI MEDICAL CENTER

发明人： Dan Gazit ,  Gadi Pelled ,  Zulma Gazit ,  Dmitriy Sheyn ,  Christopher David Hinojosa ,  Norman Wen ,  Geraldine Hamilton

IPC分类号： C12N5/077 ,  A61K49/00 ,  A61K51/02 ,  A61L27/38 ,  B01L3/00 ,  C12N5/00 ,  C12N5/071 ,  C12N5/074 ,  C12N5/0775 ,  G01N33/50 ,  G01T1/164 ,  G01T1/29 ,  C07K14/78 ,  C12M1/34

CPC分类号： B01L3/5027 ,  A61K49/0019 ,  A61K49/005 ,  A61K51/02 ,  A61L27/3895 ,  B01L3/5085 ,  C12N5/0062 ,  C12N5/0075 ,  C12N5/0654 ,  C12N5/0655 ,  C12N5/0662 ,  C12N5/0663 ,  C12N5/0668 ,  C12N5/0696 ,  C12N5/0697 ,  G01N33/5038 ,  G01T1/1644 ,  G01T1/2985 ,  A61M2205/3334 ,  C07K14/78 ,  C12M41/40

摘要： Microfluidic “organ-on-a-chip” devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.

公开(公告)号：US20240124822A1

公开(公告)日：2024-04-18

申请号：US17953889

申请日：2022-09-27

申请人： EMULATE, Inc.

发明人： Christopher David Hinojosa ,  Josiah Sliz ,  Iosif Pediaditakis ,  Sonalee Barthakur

IPC分类号： C12M1/00 ,  B01L3/00 ,  C12M3/06

CPC分类号： C12M29/10 ,  B01L3/502738 ,  C12M23/16 ,  C12M29/14 ,  B01L2300/0861 ,  B01L2400/0605 ,  G01N1/18

摘要： The invention generally relates to a microfluidic platforms or “chips” for testing and conducting experiments on the International Space Station (ISS). More specifically, microfluidic Brain-On-Chip, comprising neuronal and vascular endothelial cells, will be analyzed in both healthy and inflamed states to assess how the circumstances of space travel affect the human brain.

公开(公告)号：US11920114B2

公开(公告)日：2024-03-05

申请号：US17176873

申请日：2021-02-16

申请人： EMULATE, Inc.

发明人： Daniel Levner ,  Josiah Daniel Sliz ,  Christopher David Hinojosa ,  Joshua Gomes ,  Jose Fernandez-Alcon

IPC分类号： C12M3/06 ,  A01N1/02 ,  B01L3/00 ,  B01L9/00 ,  C12M1/00 ,  C12M1/34 ,  C12M1/36 ,  C12M1/42 ,  C12M3/00 ,  C12N5/071

CPC分类号： C12M23/16 ,  A01N1/021 ,  A01N1/0247 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502738 ,  B01L9/527 ,  C12M21/08 ,  C12M23/38 ,  C12M23/40 ,  C12M23/42 ,  C12M29/10 ,  C12M35/04 ,  C12M41/40 ,  C12M41/48 ,  C12N5/0602 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0887 ,  B01L2300/123 ,  B01L2300/14 ,  B01L2300/161 ,  B01L2300/165 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/06 ,  C12N2521/00

摘要： A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

公开(公告)号：US20230022203A1

公开(公告)日：2023-01-26

申请号：US17950422

申请日：2022-09-22

申请人： EMULATE, Inc

发明人： Daniel Levner ,  Josiah Daniel Sliz ,  Christopher David Hinojosa ,  Joshua Gomes ,  Jose Fernandez-Alcon

IPC分类号： C12M3/06 ,  B01L9/00 ,  C12M1/42 ,  C12N5/071 ,  C12M1/34 ,  A01N1/02 ,  B01L3/00 ,  C12M1/00 ,  C12M3/00 ,  C12M1/36

摘要： A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body. A method for pressure control is contemplated to allow the control of flow rate (while perfusing cells) despite limitations of common pressure regulators. The method for pressure control allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly, so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.

公开(公告)号：US20220403312A9

公开(公告)日：2022-12-22

申请号：US16730448

申请日：2019-12-30

申请人： EMULATE, Inc.

发明人： Christopher David Hinojosa ,  Guy Robert Thompson ,  Joshua Gomes ,  Jacob Freake ,  Doug Sabin

IPC分类号： C12M3/06 ,  B01L9/00 ,  C12M1/36 ,  C12M3/00 ,  C12M1/00 ,  B01L3/00 ,  A01N1/02 ,  C12N5/071 ,  C12M1/34 ,  C12M1/42

摘要： A perfusion manifold assembly is described that allows for perfusion of a microfluidic device, such as an organ on a chip microfluidic device comprising cells that mimic cells in an organ in the body, that is detachably linked with said assembly so that fluid enters ports of the microfluidic device from a fluid reservoir, optionally without tubing, at a controllable flow rate.
A culture module is contemplated that allows the perfusion and optionally mechanical actuation of one or more microfluidic devices, such as organ-on-a-chip microfluidic devices comprising cells that mimic at least one function of an organ in the body.

公开(公告)号：US11174462B2

公开(公告)日：2021-11-16

申请号：US15955335

申请日：2018-04-17

申请人： EMULATE, Inc. ,  Cedars-Sinai Medical Center

发明人： S. Jordan Kerns ,  Norman Wen ,  Carolina Lucchesi ,  Christopher David Hinojosa ,  Jacob Fraser ,  Geraldine Hamilton ,  Gad Vatine ,  Samuel Sanees ,  Clive Svendsen ,  Daniel Levner ,  Dhruv Sareen

IPC分类号： C12N5/0793 ,  C12N5/071 ,  C12N5/079 ,  C12Q1/02 ,  C12M1/12 ,  C12M1/42 ,  C12M3/06 ,  B01L3/00

摘要： The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.