公开(公告)号：US20130244908A1

公开(公告)日：2013-09-19

申请号：US13889025

申请日：2013-05-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jonathan M. ROTHBERG ,  Wolfgang HINZ ,  Kim L. JOHNSON ,  James BUSTILLO

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  G01N27/414 ,  G01N27/4145 ,  G01N27/4148 ,  H01L21/306 ,  H01L24/18 ,  H01L24/20 ,  H01L24/82 ,  H01L27/088 ,  H01L29/78 ,  H01L2224/04105 ,  H01L2224/16 ,  H01L2224/76155 ,  H01L2224/82102 ,  H01L2924/01005 ,  H01L2924/01006 ,  H01L2924/01011 ,  H01L2924/01012 ,  H01L2924/01013 ,  H01L2924/01015 ,  H01L2924/01019 ,  H01L2924/0102 ,  H01L2924/01023 ,  H01L2924/01024 ,  H01L2924/01027 ,  H01L2924/01029 ,  H01L2924/0103 ,  H01L2924/01033 ,  H01L2924/01046 ,  H01L2924/01047 ,  H01L2924/01052 ,  H01L2924/01056 ,  H01L2924/01059 ,  H01L2924/01073 ,  H01L2924/01074 ,  H01L2924/01075 ,  H01L2924/01082 ,  H01L2924/1306 ,  H01L2924/13091 ,  H01L2924/14 ,  H01L2924/1433 ,  H01L2924/19041 ,  H01L2924/19042 ,  H01L2924/19043 ,  H01L2924/30105 ,  H01L2924/3011 ,  H01L2924/3025 ,  C12Q2565/607 ,  C12Q2565/301 ,  C12Q2533/101 ,  H01L2924/00

Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis.

Abstract translation: 与用于分析物测量的非常大规模的FET阵列相关的方法和装置。 可以使用基于提高测量灵敏度和精度的改进的FET像素和阵列设计的传统CMOS处理技术来制造ChemFET（例如，ISFET）阵列，并且同时促进显着小的像素尺寸和致密阵列。 改进的阵列控制技术提供了从大型和密集阵列的快速数据采集。 可以使用这样的阵列来检测各种化学和/或生物过程中各种分析物类型的存在和/或浓度变化。 在一个实例中，chemFET阵列基于监测氢离子浓度（pH），其他分析物浓度变化和/或与DNA合成相关的化学过程相关联的结合事件的变化来促进DNA测序技术。

公开(公告)号：US20200239877A1

公开(公告)日：2020-07-30

申请号：US16841546

申请日：2020-04-06

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  John LEAMON ,  David LIGHT ,  Jonathan M. ROTHBERG

IPC: C12N15/10 ,  C12Q1/6874 ,  C40B50/06 ,  C40B40/08 ,  B01J19/00

Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

公开(公告)号：US20190194744A1

公开(公告)日：2019-06-27

申请号：US16292087

申请日：2019-03-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  David LIGHT ,  Todd REARICK ,  James A. BALL

IPC: C12Q1/6874 ,  G01N27/414

CPC classification number: C12Q1/6874 ,  G01N27/4145

Abstract: The disclosure relates to novel particle compositions and methods of making said compositions having applications in nucleic acid analysis, as well as apparatuses and systems for the same.

公开(公告)号：US20190071667A1

公开(公告)日：2019-03-07

申请号：US16121615

申请日：2018-09-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  John LEAMON ,  David LIGHT ,  Jonathan M. ROTHBERG

IPC: C12N15/10 ,  C40B50/06 ,  C40B40/08 ,  B01J19/00 ,  C12Q1/6874

Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

公开(公告)号：US20180217092A1

公开(公告)日：2018-08-02

申请号：US15831141

申请日：2017-12-04

Applicant: Life Technologies Corporation

Inventor： Wolfgang HINZ ,  John Matthew MAURO ,  Shifeng LI ,  James BUSTILLO

IPC: G01N27/414 ,  C12Q1/6874 ,  G01N27/26 ,  H01L29/78

CPC classification number: G01N27/4148 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/414 ,  G01N27/4145 ,  H01L29/78 ,  Y10T436/143333

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

公开(公告)号：US20170292156A1

公开(公告)日：2017-10-12

申请号：US15625800

申请日：2017-06-16

Applicant: Life Technologies Corporation

Inventor： John DAVIDSON ,  Wolfgang HINZ ,  Jonathan ROTHBERG ,  Richard WHITAKER

IPC: C12Q1/68 ,  C12N9/12 ,  G01N27/414

CPC classification number: C12Q1/6869 ,  C12N9/1252 ,  C12Y207/07007 ,  G01N27/4145

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.

公开(公告)号：US20170038334A1

公开(公告)日：2017-02-09

申请号：US15331779

申请日：2016-10-21

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Kristopher BARBEE ,  John DAVIDSON ,  Wolfgang HINZ ,  Shifeng LI ,  James BUSTILLO

IPC: G01N27/447 ,  C12Q1/68 ,  G01N27/414

CPC classification number: G01N27/4473 ,  B01J19/0046 ,  B01J2219/00286 ,  B01J2219/00317 ,  B01J2219/00459 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00704 ,  B01J2219/00722 ,  C12Q1/6874 ,  G01N27/4145 ,  G01N27/4148 ,  G01N27/44743

Abstract: An apparatus includes a device substrate including an array of sensors. Each sensor of the array of sensors can include a electrode structure disposed at a surface of the device substrate. The apparatus further includes a wall structure overlying the surface of the device substrate and defining an array of wells at least partially corresponding with the array of sensors. The well structure including an electrode layer and an insulative layer.

Abstract translation: 一种装置包括包括传感器阵列的装置基板。 传感器阵列的每个传感器可以包括设置在器件基板的表面处的电极结构。 该装置还包括覆盖在装置基板的表面上的壁结构，并且限定至少部分对应于传感器阵列的一组阱阵列。 阱结构包括电极层和绝缘层。

公开(公告)号：US20170030854A1

公开(公告)日：2017-02-02

申请号：US15222492

申请日：2016-07-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Creighton BUIE ,  John DONOHUE ,  Chiu Tai Andrew WONG ,  James A. BALL ,  Wolfgang HINZ ,  James BUSTILLO ,  Jordan OWENS ,  Shifeng LI

IPC: G01N27/327 ,  C12Q1/68

CPC classification number: C12Q1/6874 ,  G01N27/3276

Abstract: Provided herein is a sensor comprising a substrate having a first reaction region and a second reaction region, a first electrode associated with the first reaction region, a second electrode associated with the second reaction region and a third electrode wherein the third electrode is common to both the first reaction region and the second reaction region.

Abstract translation: 本文提供了一种传感器，其包括具有第一反应区域和第二反应区域的基底，与第一反应区域相关联的第一电极，与第二反应区域相关联的第二电极和第三电极，其中第三电极是共同的 第一反应区和第二反应区。

公开(公告)号：US20160305905A1

公开(公告)日：2016-10-20

申请号：US15194321

申请日：2016-06-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  John Matthew MAURO ,  Shifeng LI ,  James BUSTILLO

IPC: G01N27/414 ,  C12Q1/68

CPC classification number: G01N27/4148 ,  C12Q1/6874 ,  G01N27/26 ,  G01N27/414 ,  G01N27/4145 ,  H01L29/78 ,  Y10T436/143333

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

公开(公告)号：US20160194629A1

公开(公告)日：2016-07-07

申请号：US14987552

申请日：2016-01-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Wolfgang HINZ ,  John LEAMON ,  David LIGHT ,  Jonathan M. ROTHBERG

IPC: C12N15/10 ,  C12Q1/68 ,  B01J19/00

CPC classification number: C12N15/1093 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/005 ,  B01J2219/00596 ,  B01J2219/00722 ,  C12Q1/6874 ,  C40B40/08 ,  C40B50/06 ,  Y10T428/2982

Abstract: The invention provides particle compositions having applications in nucleic acid analysis. Nucleic acid polymer particles of the invention allow polynucleotides to be attached throughout their volumes for higher loading capacities than those achievable solely with surface attachment. In one aspect, nucleic acid polymer particles of the invention comprise polyacrylamide particles with uniform size distributions having low coefficients of variations, which result in reduced particle-to-particle variation in analytical assays. Such particle compositions are used in various amplification reactions to make amplicon libraries from nucleic acid fragment libraries.

Abstract translation: 本发明提供了在核酸分析中具有应用的颗粒组合物。 本发明的核酸聚合物颗粒允许多核苷酸在其整个体积中被连接，以便比仅通过表面附着可实现的更高的负载能力。 在一个方面，本发明的核酸聚合物颗粒包含具有低变异系数的具有均匀尺寸分布的聚丙烯酰胺颗粒，这导致分析测定中粒子与粒子变化的减少。 这种颗粒组合物用于各种扩增反应以制备来自核酸片段文库的扩增子文库。