公开(公告)号：US10964517B2

公开(公告)日：2021-03-30

申请号：US17014264

申请日：2020-09-08

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Ahmed Mohamed Hamid ,  Alan Keith Jarmusch ,  Zheng Ouyang

IPC: H01J49/04 ,  C12N13/00 ,  C12Q1/04 ,  H01J49/16

Abstract: The invention generally relates to systems and methods for mass spectrometry analysis of microorganisms in samples.

公开(公告)号：US20210045662A1

公开(公告)日：2021-02-18

申请号：US17066727

申请日：2020-10-09

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Zheng Ouyang ,  Chien-hsun Chen ,  Ziqing Lin ,  Livia Schiavinato Eberlin

IPC: A61B5/1477 ,  A61B10/00 ,  H01J49/04 ,  A61M11/00 ,  A61M25/00 ,  H01J49/00 ,  H01J49/16 ,  H01J49/34 ,  G01N33/483

Abstract: The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

公开(公告)号：US10799165B2

公开(公告)日：2020-10-13

申请号：US16720991

申请日：2019-12-19

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Zheng Ouyang ,  Chien-hsun Chen ,  Ziqing Lin ,  Livia Schiavinato Eberlin

IPC: H01J49/00 ,  A61B5/1477 ,  A61B10/00 ,  H01J49/04 ,  A61M11/00 ,  A61M25/00 ,  H01J49/16 ,  H01J49/34 ,  G01N33/483 ,  A61B5/145 ,  A61B5/15 ,  A61B5/157 ,  G01N1/02

Abstract: The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

公开(公告)号：US10381209B2

公开(公告)日：2019-08-13

申请号：US15548275

申请日：2016-02-08

Applicant: Purdue Research Foundation ,  Zheng Ouyang ,  Yue Ren ,  Xiao Wang

Inventor： Zheng Ouyang ,  Yue Ren ,  Xiao Wang

IPC: H01J49/04 ,  H01J49/16

Abstract: The invention generally relates to probes, systems, cartridges, and methods of use thereof. In certain embodiments, the invention provides a probe including a porous material and a hollow member coupled to a distal portion of the porous material.

公开(公告)号：US20190167164A1

公开(公告)日：2019-06-06

申请号：US16248066

申请日：2019-01-15

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Zheng Ouyang ,  Chien-hsun Chen ,  Ziqing Lin ,  Livia Schiavinato Eberlin

IPC: A61B5/1477 ,  A61B10/00 ,  H01J49/34 ,  A61M11/00 ,  A61M25/00 ,  G01N33/483 ,  H01J49/00 ,  H01J49/04 ,  H01J49/16

CPC classification number: A61B5/1477 ,  A61B5/1405 ,  A61B5/145 ,  A61B5/150015 ,  A61B5/150992 ,  A61B5/157 ,  A61B10/0045 ,  A61M11/00 ,  A61M25/00 ,  A61M2202/0468 ,  G01N33/4833 ,  G01N2001/028 ,  H01J49/0022 ,  H01J49/0404 ,  H01J49/0445 ,  H01J49/167 ,  H01J49/34

Abstract: The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

公开(公告)号：US10290483B2

公开(公告)日：2019-05-14

申请号：US15988698

申请日：2018-05-24

Applicant: Purdue Research Foundation

Inventor： Zheng Ouyang ,  Robert Graham Cooks ,  Sandilya Venkata Garimella ,  Jason David Harper ,  Nicholas Alan Charipar

IPC: H01J49/26 ,  H01J49/40 ,  H01J49/04 ,  H01J49/00 ,  G01N27/62 ,  H01J49/42 ,  H01J49/10

Abstract: The invention generally relates to systems and methods for transferring ions for analysis. In certain embodiments, the invention provides a system for analyzing a sample including an ionizing source for converting molecules of a sample into gas phase ions in a region at about atmospheric pressure, an ion analysis device, and an ion transfer member operably coupled to a gas flow generating device, in which the gas flow generating device produces a laminar gas flow that transfers the gas phase ions through the ion transfer member to an inlet of the ion analysis device.

公开(公告)号：US10213143B2

公开(公告)日：2019-02-26

申请号：US15989706

申请日：2018-05-25

Applicant: Purdue Research Foundation

Inventor： Robert Graham Cooks ,  Zheng Ouyang ,  Chien-Hsun Chen ,  Ziqing Lin ,  Livia Schiavinato Eberlin

IPC: H01J49/00 ,  A61B5/1477 ,  G01N33/483 ,  H01J49/34 ,  A61M11/00 ,  A61M25/00 ,  H01J49/04 ,  H01J49/16 ,  A61B10/00 ,  A61B5/15 ,  G01N1/02 ,  A61B5/157 ,  A61B5/145

Abstract: The invention generally relates to enclosed desorption electrospray ionization probes, systems, and methods. In certain embodiments, the invention provides a source of DESI-active spray, in which a distal portion of the source is enclosed within a transfer member such that the DESI-active spray is produced within the transfer member.

公开(公告)号：US20180294148A1

公开(公告)日：2018-10-11

申请号：US15577516

申请日：2016-05-27

Applicant: Purdue Research Foundation

Inventor： Zheng Ouyang ,  Yu Xia ,  Xiaoxiao Ma

IPC: H01J49/04 ,  G01N1/30 ,  G01N30/72 ,  G01N30/88 ,  H01J49/00 ,  G01N33/92

Abstract: The invention generally relates to methods for analyzing a tissue sample. In certain aspects, the invention provides methods that involve obtaining a tissue sample including an unsaturated compound, conducting a radical reaction on the tissue sample that targets a carbon-carbon double bond within the unsaturated compound to thereby produce a plurality of compound isomers, subjecting the plurality of compound isomers to mass spectrometry analysis to identify a location of the carbon-carbon double bond within the unsaturated compound, and quantifying the plurality of compound isomers in order to distinguish normal tissue from diseased tissue.

公开(公告)号：US20180275118A1

公开(公告)日：2018-09-27

申请号：US15762597

申请日：2016-09-24

Applicant: Purdue Research Foundation

Inventor： Baird Zane ,  Robert Graham Cooks ,  Adam Hollerbach ,  Zheng Ouyang ,  Michael Pugia

IPC: G01N33/53

CPC classification number: G01N33/5302 ,  G01N2458/15

Abstract: The invention generally relates to mass tag analysis for rare cells and cell free molecules. In certain embodiments, the invention provides an apparatus including an essentially non-absorbent membrane having at least one pore, a microwell operably associated with the essentially non-absorbent membrane, and an electric field generator. The apparatus may be configured such that an electric field produced by the electric field generator operably interacts with a sample in the microwell and expels a droplet of the sample through the at least one pore in the essentially non-absorbent membrane. In certain embodiments, apparatuses of the invention are used for detection, and optionally quantification, of a target analyte from a heterogeneous sample, such as a rare target analyte (e.g., rare cell) from a biological sample.

公开(公告)号：US10008375B2

公开(公告)日：2018-06-26

申请号：US14426591

申请日：2014-01-10

Applicant: Purdue Research Foundation

Inventor： Zheng Ouyang ,  Yue Ren ,  Jiangjiang Liu ,  Linfan Li

IPC: H01J49/00 ,  H01J49/04 ,  H01J49/16 ,  H01J49/42

CPC classification number: H01J49/0409 ,  H01J49/0431 ,  H01J49/0459 ,  H01J49/167 ,  H01J49/4205

Abstract: The invention generally relates to systems for analyzing a sample and methods of use thereof. In certain aspects, the invention provides systems that include an ionization probe and a mass analyzer. The probe includes a hollow body that has a distal tip. The probe also includes a substrate that is at least partially disposed within the body and positioned prior to the distal tip so that sample extracted from the substrate flows into the body prior to exiting the distal tip. The probe also includes an electrode that operably interacts with sample extracted from the substrate.