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    Diagnostic reagents based on unique sequences within the variable region of the T cell receptor and uses thereof
    25.
    发明授权
    Diagnostic reagents based on unique sequences within the variable region of the T cell receptor and uses thereof 失效
    基于T细胞受体可变区内的独特序列的诊断试剂及其用途

    公开(公告)号：US4886743A

    公开(公告)日：1989-12-12

    申请号：US726502

    申请日：1985-04-24

    申请人： Leroy E. Hood ,  Irving L. Weissman ,  Michael S. McGrath

    发明人： Leroy E. Hood ,  Irving L. Weissman ,  Michael S. McGrath

    IPC分类号： A61K38/00 ,  C07H21/04 ,  C07K14/00 ,  C07K14/705 ,  C07K14/725 ,  C07K16/00 ,  C07K16/28 ,  C07K19/00 ,  C12N15/00 ,  C12N15/09 ,  C12P21/08 ,  C12Q1/68 ,  G01N33/50 ,  G01N33/53 ,  G01N33/564 ,  G01N33/569 ,  G01N33/574 ,  G01N33/576 ,  G01N33/577

    CPC分类号： C07K16/2809 ,  C12Q1/6876 ,  C12Q1/6883 ,  G01N33/5091 ,  G01N33/564 ,  G01N33/56972 ,  G01N33/56988 ,  G01N33/57415 ,  G01N33/576 ,  A61K38/00 ,  G01N2800/102 ,  G01N2800/245 ,  Y10S435/974 ,  Y10S436/813 ,  Y10T436/117497

    摘要： This invention provides a reagent capable of binding to T cells and having specificity for a unique sequence within the variable region of the .beta. chain of the T cell receptor, the presence of increased number of T cells carrying the unique sequence relative to the number of T cells carrying the sequence present in a normal subject being associated with a specific disease. Specific diseases such as human cancers, e.g. lymphomas; autoimmune diseases, e.g. rheumatoid arthritis; Alzheimer's disease; infectious diseases, e.g. those caused by bacteria, yeast or parasite; or allergies, may be diagnosed as follows. A suitable sample containing T cells is obtained from a subject. The sample is contacted under appropriate conditions with such a reagent. If the subject's cells contain the unique sequence, a detectable complex is formed between the reagent and T cells which contain the sequence. By quantitatively determining the number of T cells containing the sequence present in the complex formed and comparing this number with the number of T cells carrying the sequence determined for a normal subject, the disease with which the unique sequence is associated may be diagnosed. Similar reagents and methods may also be used to detect organ transplant rejection.

    摘要翻译： 本发明提供了能够结合T细胞并且对T细胞受体的β链的可变区内的唯一序列具有特异性的试剂，相对于T细胞数目的携带具有唯一序列的T细胞数量的增加 携带存在于正常受试者中的序列的细胞与特定疾病相关。 特定疾病，如人类癌症，例如 淋巴瘤 自身免疫性疾病，例如 类风湿关节炎; 阿尔茨海默氏病; 传染病，例如 那些由细菌，酵母或寄生虫引起的; 或过敏，可以诊断如下。 从受试者获得含有T细胞的合适样品。 样品在适当的条件下与这种试剂接触。 如果受试者的细胞含有唯一的序列，则在包含该序列的试剂和T细胞之间形成可检测的复合物。 通过定量测定含有存在于所形成的复合物中的序列的T细胞的数目，并将该数目与携带为正常受试者确定的序列的T细胞的数目进行比较，可以诊断与该独特序列相关的疾病。 类似的试剂和方法也可用于检测器官移植排斥反应。

    Sandwich EIA for antigen
    27.
    发明授权
    Sandwich EIA for antigen 失效
    三明治EIA抗原

    公开(公告)号：US4642285A

    公开(公告)日：1987-02-10

    申请号：US606122

    申请日：1984-05-02

    申请人： Seymour P. Halbert ,  Milton Anken

    发明人： Seymour P. Halbert ,  Milton Anken

    IPC分类号： G01N33/576 ,  G01N33/53 ,  G01N33/535 ,  G01N33/545

    CPC分类号： G01N33/576 ,  Y10S435/805 ,  Y10S435/81 ,  Y10S436/811 ,  Y10S436/82

    摘要： A sensitive direct immunoassay system is provided for the detection of an antigen in body fluids. A single antibody which reacts with an antigen or antigens and which is bonded to an insoluble member, is incubated with a test sample. During this first period of incubation a portion of an antigen present in the test sample will combine with the antibody immobilized on the insoluble member. The antibody bonded member, to which antigen is attached, is then washed and incubated with an enzyme tagged antibody reagent. During the second incubation, the tagged antibody reacts with antigen fixed to the antibody member in the first incubation. Thus, an immobilized "sandwich" is formed of an insoluble member- antibody-antigen-enzyme tagged antibody. After the second incubation, the member is washed again to remove unreacted enzyme antibody reagent. The member is then exposed to a substrate which is converted by the enzyme to produce an end product. The tagged antibody reagent will be fixed in the second incubation only if antigen was present in the sample. The amount of enzyme tagged antitbody fixed is proportional to the amount of antigen or antigens present in the test sample up to the maximum capacity of the test. The concentration of the end product, and hence the amount of antigen or antigens, is determined by a spectrophotometer which measures the optical absorption of light by the end product. This readout is then compared against a standard value for both antigen negative and antigen positive samples.

    摘要翻译： 提供敏感的直接免疫测定系统用于检测体液中的抗原。 与抗原或抗原反应并与不溶性成分结合的单一抗体与测试样品一起温育。 在该第一次孵育期间，存在于测试样品中的部分抗原将与固定在不溶性成分上的抗体结合。 然后将抗体连接的抗体结合成员洗涤并与酶标记的抗体试剂一起温育。 在第二次孵育期间，标记的抗体在第一次孵育中与固定在抗体成员上的抗原反应。 因此，固定的“夹心”由不溶性成员 - 抗体 - 抗原 - 酶标记的抗体形成。 第二次孵育后，再次洗涤成员以除去未反应的酶抗体试剂。 然后将该构件暴露于由酶转化以产生最终产物的底物。 只有抗原存在于样品中时，标记的抗体试剂才能在第二次孵育中固定。 固定的酶标抗体的量与测试样品中存在的抗原或抗原的量成比例，直到测试的最大容量。 最终产物的浓度，因此抗原或抗原的量由分光光度计测定，该分光光度计测量最终产物对光的光吸收。 然后将该读数与抗原阴性和抗原阳性样品的标准值进行比较。

    Test set for detecting the presence of antigens associated with hepatitis
    28.
    发明授权
    Test set for detecting the presence of antigens associated with hepatitis 失效
    用于检测与肝炎相关的抗原存在的检测装置

    公开(公告)号：US4157280A

    公开(公告)日：1979-06-05

    申请号：US617745

    申请日：1975-09-29

    申请人： Seymour P. Halbert ,  Milton Anken

    发明人： Seymour P. Halbert ,  Milton Anken

    IPC分类号： G01N33/545 ,  G01N33/576 ,  G01N31/14 ,  G01N33/16

    CPC分类号： G01N33/576 ,  G01N33/545 ,  Y10S436/82

    摘要： A test set useful for detecting the presence of antigens associated with hepatitis comprising an insoluble, polymeric disc-like solid having antibody reactive with antigens associated with hepatitis bonded thereto, an enzyme-hepatitis antibody conjugate reagent, an enzyme substrate solution, control sera negative, weakly positive, and strongly positive for antigens associated with hepatitis, an enzyme substrate buffer, and a horse globulin solution. Preferably, the enzyme of the conjugate is alkaline phosphatase, the enzyme substrate is p-nitrophenylphosphate and the substrate buffer has a pH on the order of 9.8.

    摘要翻译： 一种用于检测与肝炎相关的抗原存在的测试集，其包含不溶性聚合物盘状固体，其具有与与其结合的肝炎相关的抗原反应的抗体，酶 - 肝炎抗体缀合试剂，酶底物溶液，对照血清阴性， 弱阳性，与肝炎相关抗原，酶底物缓冲液和马球蛋白溶液强阳性。 优选地，缀合物的酶是碱性磷酸酶，酶底物是对硝基苯基磷酸盐，底物缓冲液的pH值为9.8。

    Method of detecting hepatitis B core antigen and antibody
    29.
    发明授权
    Method of detecting hepatitis B core antigen and antibody 失效
    检测乙型肝炎核心抗原和抗体的方法

    公开(公告)号：US4100267A

    公开(公告)日：1978-07-11

    申请号：US618644

    申请日：1975-10-01

    申请人： Eugene Douglas Shaw

    发明人： Eugene Douglas Shaw

    IPC分类号： A61K39/00 ,  A61K39/29 ,  C07K14/02 ,  G01N33/564 ,  G01N33/576 ,  A61K39/42 ,  A61K43/00 ,  G01N31/00

    CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/29 ,  G01N33/576 ,  A61K39/00 ,  C12N2730/10122 ,  C12N2730/10134 ,  C12N2770/32422 ,  C12N2770/32434 ,  Y10S436/82

    摘要： The formation of antibodies to the A-2 plaque virus previously propagated in tissue culture is described. The application describes an immune response against those antibodies and against the A-2 plaque virus antigen which is associated with human viral hepatitis both of type A and B origin. The reaction of antibody for A-2 plaque virus with the antigen from stool specimens of volunteers infected with type A hepatitis and from the sera of patients diagnosed as having type B hepatitis is described. Convalescent sera from both type A and type B hepatitis patients in reaction with the virus, demonstrate the presence of antigenic components in the plaque virus which react with human antibody formed in response to infection with either type A or type B hepatitis. Moreover, the A-2 plaque antibodies react with hepatitis B core antigen, while antibodies to HB.sub.c Ag react with A-2 plaque antigen. Additionally, methods for inducing antibody formation in humans from the A-2 plaque virus antigen are described, as are methods for detecting HB.sub.c Ag, HB.sub.c Ab, HAAg and HAAb.

    摘要翻译： 描述了先前在组织培养中传播的对A-2斑块病毒的抗体的形成。 该应用描述了针对A型和B型来源的人类病毒性肝炎相关的针对这些抗体和针对A-2斑块病毒抗原的免疫应答。 描述了A-2斑块病毒抗体与感染A型肝炎的志愿者粪便标本和诊断为B型肝炎的患者血清的抗原反应。 A型和B型肝炎患者与病毒反应的恢复期血清证实在与A型或B型肝炎感染反应形成的人类抗体相互作用的斑块病毒中存在抗原成分。 此外，A-2斑块抗体与乙型肝炎核心抗原反应，而HBcAg的抗体与A-2斑块抗原反应。 此外，还描述了从A-2斑块病毒抗原诱导人的抗体形成的方法，以及用于检测HBcAg，HBcAb，HAAg和HAAb的方法。

    Direct radioimmunoassay for antigens and their antibodies
    30.
    发明授权
    Direct radioimmunoassay for antigens and their antibodies 失效
    用于抗原及其抗体的直接放射免疫测定

    公开(公告)号：US4012494A

    公开(公告)日：1977-03-15

    申请号：US480180

    申请日：1974-06-17

    申请人： Chung-Mei Ling

    发明人： Chung-Mei Ling

    IPC分类号： G01N33/543 ,  G01N33/576 ,  G01N33/00 ,  G01N33/16 ,  G21H5/02

    CPC分类号： G01N33/576 ,  G01N33/54306 ,  Y10S436/804 ,  Y10S436/81 ,  Y10S436/82

    摘要： A method for direct radioimmunoassay of antigens or their associated antibodies utilizing a coated test apparatus comprising adding a serum to be tested for an antigen to test apparatus coated with its antibody; incubating the tubes for from 0.5 to 42 hours; aspirating the contents and washing the same with a Tris-HCl and sodium azide mixture; adding purified I.sup.125 labeled antibody into the tube and incubating for from 1 to 24 hours; aspirating and washing the contents; and counting the tube for I.sup.125 radiation. A similar assay for the antibody may be conducted utilizing an antigen coated apparatus and I.sup.125 labeled antigen. The method for coating these apparatus such as tubes comprises adding the antigen or antibody solution in a Tris-HCl, sodium azide solution into a suitable test tube or well, incubating the tubes at room temperature, aspirating and washing the contents and storing at from between 2.degree. and 25.degree. C. until use.

    摘要翻译： 一种用于使用包被的测试装置直接放射免疫测定抗原或其相关抗体的方法，包括将待测试的血清加入到其抗体包被的测试仪器上; 孵育管0.5至42小时; 吸入内容物并用Tris-HCl和叠氮化钠混合物洗涤; 将纯化的I125标记的抗体加入管中并孵育1至24小时; 吸取和洗涤内容物; 并计数I125辐射管。 可以使用抗原包被的设备和I125标记的抗原来进行抗体的类似测定。 用于涂布这些装置如管的方法包括将抗原或抗体溶液加入到Tris-HCl，叠氮化钠溶液中至合适的试管或孔中，在室温下孵育该管，抽吸和洗涤内容物并从其间储存 2℃和25℃直至使用。