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146 条记录 (耗时 0.031 秒)

    Vector constructs for the selection and identification of open reading frames
    22.
    发明授权
    Vector constructs for the selection and identification of open reading frames 失效
    用于选择和识别开放阅读框架的向量结构

    公开(公告)号：US5981182A

    公开(公告)日：1999-11-09

    申请号：US816721

    申请日：1997-03-13

    申请人： William R. Jacobs, Jr. ,  Sabine Daugelat

    发明人： William R. Jacobs, Jr. ,  Sabine Daugelat

    IPC分类号： C12N1/11 ,  C12N15/10 ,  C12Q1/68 ,  C07H21/04 ,  C12N15/63 ,  C12P21/02

    CPC分类号： C12N15/1051 ,  C12N15/1034 ,  C12Q1/6897

    摘要： The present invention provides for novel vector constructs comprising an origin of replication; a nucleotide sequence encoding an intein, the nucleotide sequence having a unique restriction enzyme site, critical amino acid residues located at the splice junctions of the intein, the intein inserted into a nucleotide sequence encoding a selectable marker; and a nucleotide sequence encoding suitable regulatory elements so as to effect expression of the vector construct in a suitable host cell. The vector constructs of the present invention may contain a DNA of interest cloned into a unique restriction site of the intein, and may be used as a vaccine alone or transformed into a vaccine vector. The vector constructs of this invention may further be used in methods of selecting translated open reading frames or genes, leading to the identification of potentially protective antigens of pathogenic organisms.

    摘要翻译： 本发明提供了包含复制起点的新载体构建体; 编码内含肽的核苷酸序列，具有独特的限制酶位点的核苷酸序列，位于内含肽剪接点的关键氨基酸残基，插入编码可选择标记的核苷酸序列的内含肽; 以及编码合适的调控元件的核苷酸序列，以便在合适的宿主细胞中影响载体构建体的表达。 本发明的载体构建体可以含有克隆到内含肽的独特的限制性位点的目标DNA，并且可以单独使用或转化成疫苗载体。 本发明的载体构建体还可用于选择翻译的开放阅读框或基因的方法，导致鉴定病原体的潜在保护性抗原。

    DNA encoding an insect gamma-aminobutyric acid (GABA) receptor subunit cells expressing it, and pesticide screening methods using such cells
    26.
    发明授权
    DNA encoding an insect gamma-aminobutyric acid (GABA) receptor subunit cells expressing it, and pesticide screening methods using such cells 失效
    编码表达它的昆虫γ-氨基丁酸（GABA）受体亚基细胞的DNA和使用这种细胞的农药筛选方法

    公开(公告)号：US5487976A

    公开(公告)日：1996-01-30

    申请号：US137614

    申请日：1993-10-15

    申请人： David M. Soderlund ,  Douglas C. Knipple ,  Joseph E. Henderson

    发明人： David M. Soderlund ,  Douglas C. Knipple ,  Joseph E. Henderson

    IPC分类号： C07K14/435 ,  C12N15/12 ,  G01N33/50 ,  G01N33/94 ,  C12N1/11 ,  C12N5/00 ,  G01N33/68

    CPC分类号： G01N33/5014 ,  C07K14/435 ,  G01N33/9426 ,  G01N2430/10

    摘要： The present invention provides for the isolation of genomic DNA fragments from Drosophila melanogaster encoding conserved amino acid sequence elements unique to the ligand-gated chloride channel gene family. A polymerase chain reaction (PCR)-based homology probing strategy was utilized to isolate the genomic DNA fragments. Using the PCR strategy, three discrete amplified sequence elements designated LCCH1, LCCH2 and LCCH3, were isolated, LCCH2 and LCCH3 being novel. In another aspect of the present invention, there is provided the cloning and characterization of a cDNA encoding a novel member of the ligand-gated chloride channel gene family of insects isolated from Drosophila melanogaster. The cDNA corresponds to the LCCH3 genomic sequence of the invention.

    摘要翻译： 本发明提供了从配体门控氯化物通道基因家族特有的编码保守氨基酸序列元件的黑腹果蝇分离基因组DNA片段。 使用基于聚合酶链反应（PCR）的同源性探测策略来分离基因组DNA片段。 使用PCR策略，分离出三个独立的扩增序列元件LCCH1，LCCH2和LCCH3，LCCH2和LCCH3是新的。 在本发明的另一方面，提供了编码从果蝇分离的昆虫的配体门控氯化物通道基因家族的新成员的cDNA的克隆和表征。 cDNA对应于本发明的LCCH3基因组序列。