公开(公告)号：US20180010105A1

公开(公告)日：2018-01-11

申请号：US15607247

申请日：2017-05-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Jeffrey ROGERS ,  Robert POTTER

IPC: C12N9/12 ,  C12P19/34 ,  C12N15/10

CPC classification number: C12N9/1276 ,  C12N15/1096 ,  C12P19/34 ,  C12Y207/07049

Abstract: The invention provides novel reverse transcriptases (RTs) with desirable properties such as increased thermostability, increased thermoreactivity and/or increased resistance to inhibitors. In certain embodiments, the invention provides methods of producing, amplifying and/or sequencing nucleic acid molecules (particularly cDNA molecules) using kits, compositions and/or reactions mixtures containing such novel reverse transcriptase enzymes.

公开(公告)号：US09864846B2

公开(公告)日：2018-01-09

申请号：US14018069

申请日：2013-09-04

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Charles Sugnet ,  Simon Cawley ,  Mohit Gupta ,  Iztok Marjanovic ,  Mark Beauchemin ,  Todd Rearick

IPC: G06F19/10 ,  G11C17/00 ,  H03M7/30 ,  G06F19/00

CPC classification number: G06F19/702 ,  G06F19/709 ,  H03M7/30

Abstract: A compression method includes: measuring a waveform associated with a chemical event occurring on a sensor array, wherein the waveform comprises a plurality of measured values and the chemical event is indicative of a number of nucleotide incorporations in a genetic sequencing reaction; applying a first compression process to the waveform, the first compression process including a truncating of data corresponding to a portion of the waveform that is not related to nucleotide incorporations in the genetic sequencing reaction; and applying a second compression process to the waveform, the second compression process including a data substitution process that replaces at least a portion of the waveform with a plurality of coefficients representative of the portion of the waveform.

公开(公告)号：US09856508B2

公开(公告)日：2018-01-02

申请号：US15345098

申请日：2016-11-07

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Rouh-Rong Juo ,  Brooks Edwards ,  Melissa Gee ,  Zhixian Wang ,  Kathleen Skaare

IPC: C12Q1/26

CPC classification number: C12Q1/26 ,  C07D305/14 ,  C07D321/00 ,  C07D323/00 ,  C07D407/04 ,  C07D409/04 ,  C07D493/04 ,  C07D493/10 ,  G01N2333/90245 ,  G01N2333/90251 ,  G01N2333/90258

Abstract: Chemiluminescent compositions, methods, assays and kits for oxidative enzymes are described. Further disclosed are dioxetane compounds of the form: where R can independently be any branched alkyl or cycloalkyl group which provides stabilization for the dioxetane or where both R groups together form a cycloalkyl or polycycloalkyl moiety spiro bound to the dioxetane ring, wherein each R group or the spiro bound moiety can be unsubstituted or substituted with one or more electron-withdrawing groups or electron donating groups, or groups providing preferential oxidative isozyme substrate recognition, and wherein R1 is an aryl group, or an alkyl group of 1-20 carbon atoms, which can be optionally substituted with 1 or more halogen atoms, and wherein T is an aryl or heteroaryl ring capable of emitting light upon enzyme activated decomposition of the dioxetane I. Kits, methods and assays are also disclosed that comprise the dioxetane compounds.

公开(公告)号：US09852919B2

公开(公告)日：2017-12-26

申请号：US14676697

申请日：2015-04-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Christina E. Inman ,  Alexander Mastroianni ,  Wolfgang Hinz ,  Shifeng Li ,  Scott C. Benson

IPC: H01L21/00 ,  H01L21/311 ,  G01N27/414

CPC classification number: H01L21/31105 ,  G01N27/4145 ,  H01L21/31144

Abstract: A method of manufacturing a sensor, the method including forming an array of chemically-sensitive field effect transistors (chemFETs), depositing a dielectric layer over the chemFETs in the array, depositing a protective layer over the dielectric layer, etching the dielectric layer and the protective layer to form cavities corresponding to sensing surfaces of the chemFETs, and removing the protective layer. The method further includes, etching the dielectric layer and the protective layer together to form cavities corresponding to sensing surfaces of the chemFETs. The protective layer is at least one of a polymer, photoresist material, noble metal, copper oxide, and zinc oxide. The protective protective layer is removed using at least one of sodium hydroxide, organic solvent, aqua regia, ammonium carbonate, hydrochloric acid, acetic acid, and phosphoric acid.

公开(公告)号：US09841398B2

公开(公告)日：2017-12-12

申请号：US13736566

申请日：2013-01-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： James Bustillo ,  Shifeng Li

IPC: H01L29/66 ,  G01N27/414

CPC classification number: G01N27/414 ,  G01N27/4145 ,  G01N27/4148

Abstract: In one implementation, a method for manufacturing a chemical detection device is described. The method includes forming a chemical sensor having a sensing surface. A dielectric material is deposited on the sensing surface. A first etch process is performed to partially etch the dielectric material to define an opening over the sensing surface and leave remaining dielectric material on the sensing surface. An etch protect material is formed on a sidewall of the opening. A second etch process is then performed to selectively etch the remaining dielectric material using the etch protect material as an etch mask, thereby exposing the sensing surface.

公开(公告)号：US09835585B2

公开(公告)日：2017-12-05

申请号：US14197741

申请日：2014-03-05

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Keith G. Fife ,  Jordan Owens ,  Shifeng Li ,  James Bustillo

IPC: H01L27/14 ,  G01N27/414

CPC classification number: G01N27/4145

Abstract: In one implementation, a chemical sensor is described. The chemical sensor includes a chemically-sensitive field effect transistor including a floating gate conductor having an upper surface. A conductive element protrudes from the upper surface of the floating gate conductor into an opening. A dielectric material defines a reaction region. The reaction region overlies and extends below an upper surface of the conductive element.

公开(公告)号：US20170335387A1

公开(公告)日：2017-11-23

申请号：US15497872

申请日：2017-04-26

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Dumitru Brinza ,  Zheng ZHANG ,  Fiona HYLAND ,  Rajesh GOTTIMUKKALA

IPC: C12Q1/68 ,  G06F19/22 ,  G06F19/12

CPC classification number: C12Q1/6874 ,  G16B5/00 ,  G16B30/00

Abstract: Systems and method for determining variants can receive mapped reads, align flow space information to a flow space representation of a corresponding portion of the reference. Reads spanning a position with a potential variant can be evaluated in a context specific manner. A list of probable variants can be provided.

公开(公告)号：US09824180B2

公开(公告)日：2017-11-21

申请号：US14055520

申请日：2013-10-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Nils Homer

IPC: G01N33/48 ,  G06F19/22 ,  C12Q1/68 ,  G06G7/58 ,  G06F19/24

CPC classification number: G06F19/22 ,  C12Q1/6869 ,  G06F19/24 ,  C12Q2527/119 ,  C12Q2533/101 ,  C12Q2565/607

Abstract: Disclosed are new and improved methods and systems for nucleic acid sequence analysis that can analyze data indicative of natural by-products of nucleotide incorporation events without the need for exogenous labels or dyes to identify nucleic acid sequences of interest. In particular, the methods and systems of the present teachings can process such data and various forms thereof to align fragments of the nucleic acid(s) of interest, particularly those analyzed using an addition sequencing technique, for example, as occurs with the use of nucleotide flows.

公开(公告)号：US20170327902A1

公开(公告)日：2017-11-16

申请号：US15155440

申请日：2016-05-16

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： CHANG ZHONG

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6858 ,  C12Q1/6881 ,  C12Q2600/156 ,  C12Q2525/151 ,  C12Q2563/107 ,  C12Q2565/125

Abstract: Disclosed herein are methods and compositions for human identification using polymorphisms in the Penta E short tandem repeat locus. These newly disclosed polymorphisms are significant in preventing allelic drop out.

公开(公告)号：US20170313772A1

公开(公告)日：2017-11-02

申请号：US15433181

申请日：2017-02-15

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Karoline Schjetne ,  Tanja Aarvak ,  Lars Norderhaug

IPC: C07K16/28 ,  C07K16/00 ,  C12N5/0783

CPC classification number: C07K16/2803 ,  C07K16/00 ,  C07K16/2809 ,  C07K16/2896 ,  C07K2317/53 ,  C07K2317/55 ,  C12N5/0636

Abstract: Provided are methods and composition useful in isolating or otherwise preparing a population of target cells. In part, it relates to recombinant antibodies comprising an exogenous proteolytic cleavage site and nucleic acid molecules encoding the antibodies.