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公开(公告)号：US20170121766A1
公开(公告)日：2017-05-04
申请号：US15404735
申请日：2017-01-12
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jonathan SCHULTZ , John DAVIDSON
IPC: C12Q1/68
CPC classification number: C12Q1/6869 , B01L3/5027 , C12Q1/6874 , C12Q2537/155 , C12Q2563/149 , C12Q2565/301 , C12Q2565/629
Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.
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362.发明申请公开(公告)号：US20170114321A1
公开(公告)日：2017-04-27
申请号：US15354241
申请日：2016-11-17
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Ronald BERENSON , Mark BONYHADI , Dale KALAMASZ
IPC: C12N5/00 , A61K35/17 , C12N5/0783
CPC classification number: C12N5/0087 , A61K35/17 , C12N5/0636 , C12N2501/48 , C12N2502/1114 , Y02A50/475
Abstract: The present invention relates generally to methods for stimulating T cells, and more particularly, to methods to eliminate undesired (e.g., autoreactive, alloreactive, pathogenic) subpopulations of T cells from a mixed population of T cells, thereby restoring the normal immune repertoire of said T cells. The present invention also relates to compositions of cells, including stimulated T cells having restored immune repertoire and uses thereof.
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公开(公告)号：USD785009S1
公开(公告)日：2017-04-25
申请号：US29540273
申请日：2015-09-22
Applicant: LIFE TECHNOLOGIES CORPORATION
Designer： Chee Kiong Lim , Kok Siong Teo , Gary Chappell
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公开(公告)号：US09624424B2
公开(公告)日：2017-04-18
申请号：US14566166
申请日：2014-12-10
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Yi-Zhen Hu , Hee Chol Kang
IPC: C07D403/06 , C07D413/06 , C07D417/06 , C09K11/06 , C07D209/14 , C07D401/14 , C09B23/01 , C09B23/08 , G01N33/52 , C07D209/26 , C07D401/10 , C07H19/207
CPC classification number: C07H19/207 , C07D209/14 , C07D209/26 , C07D401/10 , C07D401/14 , C09B23/0041 , C09B23/0066 , C09B23/086 , C09B23/164 , C09K11/06 , C09K2211/1007 , C09K2211/1014 , C09K2211/1022 , C09K2211/1044 , C09K2211/1059 , C09K2211/1074 , G01N33/52
Abstract: Provided herein are heptamethine cyanine dyes having a large Stokes shift, and the salts and conjugates thereof. Also provided are methods of using and making such large Stokes shift dyes as fluorescence resonance energy transfer (FRET) acceptors or donors.
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公开(公告)号：US20170101668A1
公开(公告)日：2017-04-13
申请号：US15387525
申请日：2016-12-21
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Brian REED
CPC classification number: C12Q1/6806 , C07H1/06 , C12N15/1013 , C12Q1/6844 , C12Q2537/163 , C12Q2563/159
Abstract: A method of separating bead substrates includes applying an emulsion to an emulsion-breaking solution. A dispersed phase of the emulsion includes an unbound polynucleotide, a first set of bead substrates and a second set of bead substrates. The unbound polynucleotide includes a segment complementary to a coupling oligonucleotide. The first set of bead substrates includes the coupling oligonucleotide extended to include a segment complementary to a portion of the unbound polynucleotide. The second set of bead substrates includes the coupling oligonucleotide. The emulsion-breaking solution includes an interference probe having a sequence similar to the coupling oligonucleotide or complementary to the coupling oligonucleotide. The method further includes binding beads of the first set of bead substrates to separation substrates and separating unbound beads of the second set of bead substrates from the beads of the first set of bead substrates bound to the separation substrates.
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Patent Agency Ranking
公开(公告)号：USD783178S1
公开(公告)日：2017-04-04
申请号：US29526967
申请日：2015-05-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Designer： Josh Mead , Sandro Klein , Xin Mathers , Kyle Bulloch
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公开(公告)号：US09599622B2
公开(公告)日：2017-03-21
申请号：US14402358
申请日：2013-03-14
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kyle Gee , Upinder Singh , Aleksey Rukavishnikov , Daniel Beacham , Shih-Jung Huang , Michael Janes , Wenjun Zhou
CPC classification number: C09B11/24 , A61K49/0041 , C07D311/90 , G01N33/5005 , G01N33/582 , G01N33/84
Abstract: Disclosed herein are compounds, compositions, methods and kits for detecting pH in samples using pH-sensitive fluorescent dyes. The compounds disclosed herein are novel xanthene-derivative dyes comprising an aniline moiety with one or more electron donating groups, which dyes are for detecting pH in samples either in vitro or in vivo. Also described herein are processes for preparing said dyes for use in the disclosed compositions, methods and kits.
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公开(公告)号：US20170072398A1
公开(公告)日：2017-03-16
申请号：US15266943
申请日：2016-09-15
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Kuan Moon Boo , Wei Fuh Teo , Wuh Ken Loh , Zeqi Tan , Siew Yin Lee
CPC classification number: B01L7/52 , B01L2200/025 , B01L2200/04 , B01L2200/147 , B01L2300/0829 , B01L2300/1822 , F25B21/04 , F25B2321/0251
Abstract: A thermal block assembly for use in a biological analysis system includes a sample block, a heating and cooling element, a heat sink including a surface, the surface including a plurality of projections for engaging the heating and cooling element to hold the heating and cooling element on the heat sink. A thermal block assembly for use in a biological analysis system includes a heating and cooling element, a sample block including a lower surface configured to be thermally coupled to the heating and cooling element, one or more temperature sensors configured to extend through the one or more slots of the lower surface of the sample block, and one or more thermal pads between the one or more temperature sensors and heating and cooling element.
Abstract translation: 用于生物分析系统的热块组件包括样品块，加热和冷却元件，包括表面的散热器，所述表面包括多个突起，用于接合加热和冷却元件以保持加热和冷却元件 在散热片上 用于生物分析系统的热块组件包括加热和冷却元件，样品块，其包括配置成热耦合到加热和冷却元件的下表面，配置成延伸穿过所述一个或多个 样品块的下表面的槽，以及一个或多个温度传感器和加热和冷却元件之间的一个或多个热垫。
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公开(公告)号：US09580743B2
公开(公告)日：2017-02-28
申请号：US14450925
申请日：2014-08-04
Applicant: LIFE TECHNOLOGIES CORPORATION
Inventor： Jun Lee , Robert Potter , David Mandelman
CPC classification number: C12Q1/6806 , C12N9/1252 , C12N9/1276 , C12N15/62 , C12Q1/6848 , C12Q2522/101
Abstract: Fusion proteins comprising a single strand DNA binding protein and a nucleic acid polymerase (e.g. DNA polymerase or reverse transcriptase). These high fidelity proteins are suitable for use in nucleic acid amplification methods, including the polymerase chain reaction (PCR).
Abstract translation: 包含单链DNA结合蛋白和核酸聚合酶（例如DNA聚合酶或逆转录酶）的融合蛋白。 这些高保真蛋白质适用于核酸扩增方法，包括聚合酶链反应（PCR）。
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公开(公告)号：US20170051342A1
公开(公告)日：2017-02-23
申请号：US15230834
申请日：2016-08-08
Applicant: LIFE TECHNOLOGIES CORPORATION
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6853 , C12Q1/6869 , C12Q1/6874 , C12Q2525/113 , C12Q2525/125
Abstract: A composition is provided comprising a negatively charged group, an oligonucleotide sequence and at least none or one nuclease-resistant linkage group to form a chemically-enhanced primer. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the primer as well as a method of preparing DNA for sequencing and a method of sequencing DNA and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition under conditions in which excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded.
Abstract translation: 提供了一种组合物，其包含带负电荷的基团，寡核苷酸序列和至少一个或一个核酸酶抗性连锁基团以形成化学增强的引物。 化学增强引物可用于测序和片段分析。 还提供了合成引物的方法以及用于测序的DNA的制备方法和测序DNA的方法以及含有化学增强的引物的试剂盒。 DNA测序的方法可以包括使扩增反应产物与组合物在过量扩增引物被核酸酶降解的条件下接触，化学增强引物基本上不降解。
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