公开(公告)号：US20140141460A1

公开(公告)日：2014-05-22

申请号：US14089070

申请日：2013-11-25

Applicant: Advanced Liquid Logic, Inc.

Inventor： Allen E. Eckhardt

IPC: C12Q1/54

CPC classification number: C12Q1/40 ,  B01L3/502784 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/18 ,  B01L2400/0427 ,  B01L2400/043 ,  C07D311/16 ,  C07D311/18 ,  C07D311/20 ,  C07D405/12 ,  C07D413/12 ,  C07H15/203 ,  C07H17/075 ,  C12Q1/25 ,  C12Q1/34 ,  C12Q1/44 ,  C12Q1/54 ,  C12Y302/01023 ,  G01N21/6452 ,  G01N33/5438 ,  G01N2333/916 ,  G01N2333/926 ,  G01N2333/938 ,  G01N2800/04

Abstract: The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties.

Abstract translation: 本发明涉及液滴致动器装置和测定方法。 本发明包括进行测定的测定方法，其包括将样品与伞形酮基衍生物组合，其中所述样品潜在地包含能够切割伞形变体基衍生物的酶，并且其中伞形载体衍生物包含用一个或多个修饰部分修饰的伞形酮核心。

公开(公告)号：US20130178968A1

公开(公告)日：2013-07-11

申请号：US13737514

申请日：2013-01-09

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Ryan A. Sturmer ,  Gregory F. Smith ,  Keith R. Brafford ,  Michael Fogleman ,  Sai Ram Rongali

IPC: G06F19/00

CPC classification number: G06F19/00 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502792 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/089 ,  F04B17/00 ,  F04B19/006 ,  F04B49/065

Abstract: The present invention provides modified droplet actuator systems, software, and software-executed methods for use in droplet actuator operation and droplet actuator systems that are configured and programmed to execute such software. The invention provides a computer readable medium storing processor executable code for performing a method, the method comprising receiving a selection of a function to be performed by a droplet actuator, associating the function to a predefined electrode element, associating the predefined electrode element to a grouping of one or more electrodes that perform the function, and adding the grouping of one or more electrodes to an electronic layout of the droplet actuator.

Abstract translation: 本发明提供了用于在液滴致动器操作中使用的修改的液滴致动器系统，软件和软件执行方法以及配置和编程为执行这样的软件的液滴致动器系统。 本发明提供了一种存储用于执行方法的处理器可执行代码的计算机可读介质，该方法包括接收由液滴致动器执行的功能的选择，将功能与预定电极元件相关联，将预定电极元件与分组 执行功能的一个或多个电极，以及将一个或多个电极的分组添加到液滴致动器的电子布局。

公开(公告)号：US11525827B2

公开(公告)日：2022-12-13

申请号：US16811789

申请日：2020-03-06

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543 ,  B01L3/00 ,  B03C1/02 ,  B03C1/28 ,  B03C1/30 ,  B03C5/00 ,  C12N15/10 ,  G01N33/561 ,  B01F31/65 ,  B01F33/302 ,  B01F33/3031 ,  B01L7/00 ,  G01N35/10

Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.

公开(公告)号：US20190324026A1

公开(公告)日：2019-10-24

申请号：US16455506

申请日：2019-06-27

Applicant: Advanced Liquid Logic, Inc. ,  Duke University

Inventor： Ramakrishna Sista ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Richard B. Fair

IPC: G01N33/543 ,  B01L3/00 ,  G01N27/447 ,  C12M1/12

Abstract: A method comprising effecting a change in a shape of a droplet, wherein the droplet is disposed over a substrate in sensing proximity to a sensor and the droplet has a starting surface area exposed to the sensor; and producing an expanded surface area of the droplet in the sensing proximity exposed to the sensor, wherein the expanded surface area exposed to the sensor is greater than the starting surface area exposed to the sensor.

公开(公告)号：US10183292B2

公开(公告)日：2019-01-22

申请号：US14968094

申请日：2015-12-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Ryan A. Sturmer ,  Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Philip Y. Paik

IPC: B01L3/00 ,  G01N27/22 ,  G01V3/02

Abstract: A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.

公开(公告)号：US20190011443A1

公开(公告)日：2019-01-10

申请号：US16132175

申请日：2018-09-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543 ,  B03C1/28 ,  B03C1/30 ,  B03C5/00 ,  B01F11/00 ,  B01F13/00 ,  B03C1/02 ,  B01L3/00 ,  C12N15/10 ,  G01N33/561 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.

公开(公告)号：US09707579B2

公开(公告)日：2017-07-18

申请号：US15099175

申请日：2016-04-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Theodore Winger

IPC: B41J2/135 ,  B05B5/08 ,  B41J2/16 ,  B41J2/14

CPC classification number: B05B5/087 ,  B41J2/14 ,  B41J2/1606 ,  B41J2002/14322 ,  B41J2002/14395 ,  Y10T428/31504 ,  Y10T428/3154 ,  Y10T428/31855

Abstract: A microfluidic device having a substrate with an electrically conductive element made using a conductive ink layer underlying a hydrophobic layer.

公开(公告)号：US09492822B2

公开(公告)日：2016-11-15

申请号：US14942125

申请日：2015-11-16

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Ryan A. Sturmer ,  Vijay Srinivasan ,  Arjun Sudarsan

IPC: G01R27/16 ,  B01L3/00 ,  G01N27/04

CPC classification number: B01L3/50273 ,  B01L3/502792 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/087 ,  B01L2300/089 ,  B01L2400/0427 ,  G01N27/048 ,  G01R27/16

Abstract: Methods comprising measuring the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode are disclosed. Computer readable mediums storing processor executable instructions for performing the method, and systems are also disclosed. The systems comprise a processor, memory and code stored in the memory that when executed cause the processor at least to: receive an output voltage signal, superimpose an excitation signal onto the output voltage signal to produce a superimposed signal, connect the superimposed signal to an electrode in a droplet actuator, suppress the output voltage signal, when detecting an impedance of the electrode, and measure the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode.

公开(公告)号：US20160271623A1

公开(公告)日：2016-09-22

申请号：US15165883

申请日：2016-05-26

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Vamsee K. Pamula ,  Prasanna Thwar ,  Arjun Sudarsan ,  Ramakrishna Sista

IPC: B03C7/02 ,  B01L3/00

CPC classification number: B03C7/026 ,  B01L3/50273 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2300/0887 ,  B01L2400/0424 ,  B01L2400/0427 ,  B03C1/01 ,  B03C1/288 ,  B03C5/00 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N33/54326

Abstract: The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads.

Abstract translation: 本发明提供了一种在液滴中重新分布磁响应珠的方法。 该方法可以包括在液滴操作表面上使用液滴进行一个或多个液滴操作，而不从磁场的区域移除磁响应珠。 在某些情况下，液滴操作可能是电极介导的。 液滴操作可以在液滴内重新分布和/或循环磁响应珠。 在一些情况下，液滴可以包括样品液滴可以包括靶分析物。 磁响应珠的重新分布可能导致目标分析物结合磁响应珠。 在一些情况下，液滴可以包括洗涤缓冲液中的未结合物质。 磁响应珠子的重新分布导致未结合物质从磁性响应珠粒的聚集或子集的空隙中释放出来。

公开(公告)号：US20160228893A1

公开(公告)日：2016-08-11

申请号：US15099175

申请日：2016-04-14

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Theodore Winger

IPC: B05B5/08

CPC classification number: B05B5/087 ,  B41J2/14 ,  B41J2/1606 ,  B41J2002/14322 ,  B41J2002/14395 ,  Y10T428/31504 ,  Y10T428/3154 ,  Y10T428/31855

Abstract: A microfluidic device having a substrate with an electrically conductive element made using a conductive ink layer underlying a hydrophobic layer.

Abstract translation: 一种微流体装置，其具有使用在疏水层下面的导电油墨层制成的导电元件的基板。