公开(公告)号：US12005424B2

公开(公告)日：2024-06-11

申请号：US17045779

申请日：2019-04-24

Applicant: Cytiva BioProcess R&D AB

Inventor： Jonas Bengtsson ,  Linn Carlsson ,  Andreas Axen ,  Ronnie Palmgren

IPC: B01D15/08 ,  B01J20/24 ,  B01J20/26 ,  B01J20/28 ,  B01J20/285 ,  B01J20/291 ,  B01J20/30

CPC classification number: B01J20/267 ,  B01D15/08 ,  B01J20/24 ,  B01J20/28023 ,  B01J20/28047 ,  B01J20/285 ,  B01J20/291 ,  B01J20/3042 ,  B01J2220/445 ,  B01J2220/54

Abstract: The invention discloses a separation matrix comprising polysaccharide gel beads, wherein said polysaccharide gel beads comprise embedded fibers. The invention further discloses a method of preparing the separation matrix and use of the matrix for separation purposes.

公开(公告)号：US20240132538A1

公开(公告)日：2024-04-25

申请号：US17768461

申请日：2020-11-20

Applicant: Cytiva Bioprocess R&D AB

Inventor： Christopher James Sevinsky ,  Peter Lundback ,  Johan Ohman ,  Gregory Grossmann ,  Sean R. Dinn

IPC: C07K1/22 ,  C07K14/195 ,  C07K17/10

CPC classification number: C07K1/22 ,  C07K14/195 ,  C07K17/10 ,  C07K2319/92

Abstract: The present invention relates to protein purification, primarily in the chromatographic field. More closely, the invention relates to affinity chromatography using a split intein system with an improved C-intein tag and N-intein ligand, wherein the target protein may be purified as a tag-less end product with a native N-terminus.

公开(公告)号：US11820802B2

公开(公告)日：2023-11-21

申请号：US16328058

申请日：2017-09-05

Applicant: Cytiva BioProcess R&D AB

Inventor： Sophia Hober ,  Sara Kanje ,  Johan Nilvebrant

IPC: C07K14/47 ,  C07K1/22 ,  C07K14/31

CPC classification number: C07K14/4728 ,  C07K1/22 ,  C07K14/31 ,  C07K2319/20 ,  C07K2319/30 ,  C07K2319/705

Abstract: The present invention is within the field of protein engineering and purification. The invention relates to a target-binding polypeptide mutant of an IgG binding polypeptide, such as Protein A, Protein G, Protein L or Protein M, comprising a metal binding motif. More closely the invention relates to an Fc binding ligand comprising an engineered protein based on the Protein A derived Z domain, to which a calcium binding EF-loop has been introduced.

公开(公告)号：US20230347316A1

公开(公告)日：2023-11-02

申请号：US17981262

申请日：2022-11-04

Applicant: Cytiva Bioprocess R&D AB

Inventor： Martin HALL ,  Sture LARSSON ,  Andreas MURANYI ,  Gustav RODRIGO ,  Jinyu ZOU ,  Per-Mikael ABERG

IPC: B01J20/24 ,  B01D15/38 ,  B01J20/286 ,  C07K1/22 ,  B01J20/32 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  B01J20/28 ,  B01J20/285 ,  B01J20/289 ,  C07K17/10

CPC classification number: B01J20/24 ,  B01D15/3809 ,  B01J20/286 ,  C07K1/22 ,  B01J20/3274 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  B01J20/28019 ,  B01J20/285 ,  B01J20/289 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3293 ,  C07K17/10 ,  C07K2317/55 ,  B01J2220/52 ,  B01J2220/54

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIP) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates to a process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

公开(公告)号：US11753438B2

公开(公告)日：2023-09-12

申请号：US16095753

申请日：2017-05-10

Applicant: CYTIVA BIOPROCESS R&D AB

Inventor： Annika Forss ,  Gustav José Rodrigo ,  Tomas Bjorkman ,  Mats Ander ,  Jesper Ulf Hansson

IPC: C07K1/22 ,  B01J20/26 ,  B01J20/285 ,  B01J20/286 ,  B01J20/32 ,  C07K14/31 ,  C07K16/00 ,  C07K16/12 ,  C07K17/10

CPC classification number: C07K1/22 ,  B01J20/267 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3274 ,  C07K14/31 ,  C07K16/00 ,  C07K16/1271 ,  C07K17/10 ,  B01J2220/52

Abstract: The present invention concerns a method of cleaning and/or sanitizing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support. The method comprises the steps of: a) optionally purifying a mixture comprising a first immunoglobulin using the separation matrix; b) providing a cleaning liquid comprising at least 50% by volume of an aqueous alkali metal hydroxide solution; and c) cleaning and/or sanitizing the separation matrix by contacting the cleaning liquid with the separation matrix for a predetermined contact time. The alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO 51 or SEQ ID NO 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.

公开(公告)号：US20230279047A1

公开(公告)日：2023-09-07

申请号：US18298857

申请日：2023-04-11

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav José Rodrigo ,  Tomas Bjorkman ,  Mats Ander ,  Jesper Ulf Hansson

IPC: C07K1/22 ,  B01D15/38 ,  B01J20/24 ,  B01J20/28 ,  B01J20/286 ,  B01J20/32 ,  C07K14/31 ,  C07K16/00 ,  C07K16/06 ,  C07K17/10

CPC classification number: C07K1/22 ,  B01D15/3809 ,  B01J20/24 ,  B01J20/28016 ,  B01J20/28078 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3274 ,  C07K14/31 ,  C07K16/00 ,  C07K16/065 ,  C07K17/10

Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein:

a) the ligands comprise multimers of alkali-stabilized Protein A domains, and
b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.

公开(公告)号：US20230091082A1

公开(公告)日：2023-03-23

申请号：US18059929

申请日：2022-11-29

Applicant: Cytiva BioProcess R&D AB

Inventor： Martin Hall ,  Karolina Busson ,  Jean-Luc Maloisel ,  Helena Skoglar

IPC: C07K1/18 ,  B01J41/20 ,  B01D15/36 ,  B01D15/38 ,  A61K38/36 ,  A61K38/48 ,  A61K35/16 ,  C12N9/64 ,  B01D15/34 ,  C07K14/47 ,  C07K14/755 ,  C07K14/765 ,  C07K16/00

Abstract: The present invention relates to the field of chromatography. More closely, the invention relates to a chromatographic method for purification of proteins, such as Factor VIII, von Willebrand factor and Factor IX. The chromatographic method is performed on a matrix comprising an inner porous core and outer porous lid surrounding said core.

公开(公告)号：US11566082B2

公开(公告)日：2023-01-31

申请号：US15525688

申请日：2015-11-11

Applicant: Cytiva BioProcess R&D AB

Inventor： Gustav Rodrigo ,  Tomas Bjorkman ,  Mats Ander

IPC: C07K16/46 ,  B01D15/38 ,  B01J20/289 ,  B01J20/32 ,  C07K1/22 ,  C07K14/31 ,  C07K16/00 ,  C07K16/06

Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.

公开(公告)号：US11517879B2

公开(公告)日：2022-12-06

申请号：US17107600

申请日：2020-11-30

Applicant: Cytiva Bioprocess R&D AB

Inventor： Martin Hall ,  Sture Larsson ,  Andreas Muranyi ,  Gustav Rodrigo ,  Jinyu Zou ,  Per-Mikael Aberg

IPC: B01J20/24 ,  B01D15/38 ,  B01J20/286 ,  C07K1/22 ,  B01J20/32 ,  C07K14/31 ,  C07K16/00 ,  C07K17/00 ,  B01J20/28 ,  B01J20/285 ,  B01J20/289 ,  C07K17/10

Abstract: The present invention relates to a chromatography ligand, which comprises Domain C from Staphylococcus protein A (SpA), or a functional fragment or variant thereof. The chromatography ligand presents an advantageous capability of withstanding harsh cleaning in place (CIF) conditions, and is capable of binding Fab fragments of antibodies. The ligand may be provided with a terminal coupling group, such as arginine or cysteine, to facilitate its coupling to an insoluble carrier such as beads or a membrane. The invention also relates process of using the ligand in isolation of antibodies, and to a purification protocol which may include washing steps and/or regeneration with alkali.

公开(公告)号：US20220266170A1

公开(公告)日：2022-08-25

申请号：US17741394

申请日：2022-05-10

Applicant: Cytiva BioProcess R&D AB

Inventor： Jan Bergstrom ,  Gunnar Glad ,  Bo-Lennart Johansson ,  Jean-Luc Maloisel ,  Nils Norrman ,  Tobias E. Soderman

IPC: B01D15/32 ,  B01J20/285 ,  B01J20/287 ,  B01J39/26 ,  B01J20/28 ,  B01J20/32 ,  C07K1/20 ,  C07K16/00 ,  C12N7/00 ,  G01N1/40

Abstract: The present invention is within the field of chromatography. More precisely, it relates to a novel chromatography medium, namely a hydrophobic medium provided with different lids excluding molecules over a certain size due to the porosity of the hydrophobic medium and/or the porosity of the lid. The invention also relates to use of the separation medium for purification of large molecules, which do not enter the separation medium, as well as small molecules, which enter the separation medium and are eluted from there.