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公开(公告)号：US08003400B2
公开(公告)日：2011-08-23
申请号：US12326972
申请日：2008-12-03
申请人： Uwe Kobold , Thomas Duelffer , Michael Grol , Rupert Herrmann , Herbert Von der Eltz , Leopold Von Proff
发明人： Uwe Kobold , Thomas Duelffer , Michael Grol , Rupert Herrmann , Herbert Von der Eltz , Leopold Von Proff
IPC分类号： G01N33/00
CPC分类号： G01N33/82 , Y10T436/20 , Y10T436/203332
摘要： The present invention relates to a method of measuring a vitamin D metabolite in a sample, the method comprising the steps of (a) treating said sample with a vitamin D metabolite releasing reagent under conditions appropriate to release a vitamin D metabolite from vitamin D-binding protein and not to cause protein precipitation, (b) subjecting the treated sample obtained in step (a) to a chromatographic separation, and (c) measuring a vitamin D metabolite during or after said chromatographic separation. The present invention also relates to methods for determining the vitamin D status of a subject, for use in the diagnosis of disease, and to agents and kits for use in performing the methods of the invention.
摘要翻译： 本发明涉及一种测量样品中维生素D代谢物的方法，所述方法包括以下步骤：（a）在适于从维生素D结合物释放维生素D代谢物的条件下用维生素D代谢物释放试剂处理所述样品 蛋白质，不会引起蛋白质沉淀，（b）使步骤（a）中得到的处理样品进行色谱分离，和（c）在所述色谱分离期间或之后测量维生素D代谢物。 本发明还涉及确定用于疾病诊断的受试者的维生素D状态的方法以及用于实施本发明的方法的试剂和试剂盒。
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公开(公告)号：US07951598B2
公开(公告)日：2011-05-31
申请号：US12328890
申请日：2008-12-05
IPC分类号： G01N33/48
CPC分类号： G01N30/06 , G01N30/603 , G01N30/7233 , G01N33/5002 , G01N2030/027 , Y10T436/24 , Y10T436/25
摘要： The invention relates to a method for differentially hemolyzing whole blood. It discloses methods for detecting an analyte in a liquid sample known or suspected to contain red blood cells and suspected or known to contain eukaryotic cells, the method including the steps of processing the liquid sample with a membrane solubilizing agent under conditions appropriate to lyse cell membranes of red blood cells and at the same time not to cause precipitation of sample constituents, subjecting the processed sample to a chromatographic separation, and detecting the analyte. The differential hemolysis of red blood cells is of advantage in a method of detecting an analyte in a liquid sample that may contain both erythrocytes and nucleated cells. The differential solubilization of red blood cells can be easily combined with an online detection methodology, like LC-MS, and is advantageous in the detection of many analytes, e.g. in the detection of folate or of immunosuppressive drugs, like tacrolimus or sirolimus.
摘要翻译： 本发明涉及一种使全血不同溶血的方法。 它公开了用于检测已知或怀疑含有红细胞并且怀疑或已知含有真核细胞的液体样品中的分析物的方法，该方法包括在适于裂解细胞膜的条件下用膜增溶剂处理液体样品的步骤 的红细胞，同时不会引起样品成分沉淀，对经处理的样品进行色谱分离，并检测分析物。 在检测可能含有红细胞和有核细胞的液体样品中的分析物的方法中，红细胞的差异溶血是有利的。 红细胞的差异溶解可以容易地与诸如LC-MS的在线检测方法组合，并且在许多分析物的检测中是有利的，例如， 在检测叶酸或免疫抑制药物，如他克莫司或西罗莫司。
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公开(公告)号：US07897404B2
公开(公告)日：2011-03-01
申请号：US10381941
申请日：2001-08-26
申请人： Eva Hoess , Herbert Andres , Frederic Donie , Rudolf Vogel , Hans-Peter Josel , Rupert Hermann , Herbert Von Der Eltz
发明人： Eva Hoess , Herbert Andres , Frederic Donie , Rudolf Vogel , Hans-Peter Josel , Rupert Hermann , Herbert Von Der Eltz
IPC分类号： G01N30/02
CPC分类号： C07K1/1077 , A61K47/643 , A61K47/6889 , G01N33/5306 , G01N33/531 , G01N33/68 , G01N33/6845 , Y10S435/961 , Y10S435/962
摘要： The invention relates to a process for the production of a biomolecule-linker conjugate of uniform stochiometry. It especially relates to a conjugate consisting of a biomolecule of a molecular weight between 5 kD and 500 kD and a hydrophilic linker molecule said linker having a molecular weight between 1 and 15 kD and between 4 and 60 charged residues, characterized in that said conjugate comprises at least one biomolecule-linker product of uniform stoichiometry in a pre-selected amount.
摘要翻译： 本发明涉及一种制备均匀化学计量学的生物分子 - 接头共轭物的方法。 它特别涉及由分子量在5kD至500kD之间的生物分子和亲水性连接分子之间的共轭物，所述亲水性连接分子的所述连接体具有1至15kD的分子量和4至60个带电荷的残基，其特征在于所述共轭物包含 至少一种具有预选量的均匀化学计量的生物分子 - 接头产物。
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公开(公告)号：US07890268B2
公开(公告)日：2011-02-15
申请号：US11304448
申请日：2005-12-14
申请人： Thomas Koch , Roderic Fuerst , Uwe Kobold , Dieter Heindl , Herbert von der Eltz , David Gelfand , Ivo Gut , Christoph Steinbeck
发明人： Thomas Koch , Roderic Fuerst , Uwe Kobold , Dieter Heindl , Herbert von der Eltz , David Gelfand , Ivo Gut , Christoph Steinbeck
CPC分类号： G06F19/18 , C12Q1/6872
摘要： The present invention is directed to an improved analysis procedure for the comparative sequencing of nucleic acids using multistage mass spectrometry. More precisely, the invention is directed to a method enabling the de-novo sequencing of nucleic acid molecules using multistage mass spectrometry.
摘要翻译： 本发明涉及使用多级质谱法进行核酸比较测序的改进的分析程序。 更准确地说，本发明涉及一种能够使用多级质谱法进行核酸分子的即时测序的方法。
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公开(公告)号：US07863040B2
公开(公告)日：2011-01-04
申请号：US12327854
申请日：2008-12-04
CPC分类号： G01N33/5002
摘要： The present invention relates to a sampling tube for collecting and processing a whole blood sample. The sampling tube contains a reagent for differential hemolysis of whole blood, wherein said reagent for differential hemolysis comprises a chemical for differential hemolysis and an anti-coagulant, and wherein said sampling tube is a ready-to-use and single-use sampling tube. It also relates to the use of said sampling tube in the processing of a whole blood sample for liquid chromatography and also to the use of a blood sample processed in such sampling tube in a liquid chromatography-based analysis.
摘要翻译： 本发明涉及一种用于收集和处理全血样本的取样管。 采样管含有用于全血差异溶血的试剂，其中所述用于差异溶血的试剂包括用于差异溶血的化学药剂和抗凝血剂，并且其中所述采样管是即用型和一次性采样管。 它还涉及在液相色谱法的全血样品的处理中使用所述采样管以及在基于液相色谱的分析中使用在这种采样管中处理的血液样品。
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36.发明授权Mutants of pyrroloquinoline quinone dependent soluble glucose dehydrogenase 有权吡咯喹啉醌依赖性可溶性葡萄糖脱氢酶的突变体公开(公告)号：US07732179B2
公开(公告)日：2010-06-08
申请号：US12250241
申请日：2008-10-13
CPC分类号： C12N9/0006
摘要： A mutant of PQQ-dependent soluble glucose dehydrogenase (s-GDH; EC 1.1.5.2) is provided with improved specificity for glucose as compared to maltose, having a substitution of threonine at position 348 by either glycine, alamine or serine, wherein said mutant additionally comprises, at least one mutation for improving the stability of the mutant and one or more mutation(s) for improving the affinity of the mutant to glucose, and/or one or more mutation(s) for further improving the specificity of the mutant for glucose as compared to maltose, and wherein position 348 correspond to the amino acid positions known from the A. calcoaceticus s-GDH wild-type sequence. Also disclosed are genes encoding such mutant s-GDH, and different applications of these s-GDH mutants, particularly for determining the concentration of glucose in a sample.
摘要翻译： 与麦芽糖相比，PQQ依赖性可溶性葡萄糖脱氢酶（s-GDH; EC 1.1.5.2）的突变体具有改善的葡萄糖特异性，其具有通过甘氨酸，丙氨酸或丝氨酸取代位置348处的苏氨酸，其中所述突变体 还包含至少一个用于提高突变体的稳定性的突变和用于提高突变体对葡萄糖的亲和力的一个或多个突变，和/或用于进一步提高突变体的特异性的一个或多个突变 用于与麦芽糖相比的葡萄糖，并且其中位置348对应于从A.calcoaceticus s-GDH野生型序列已知的氨基酸位置。 还公开了编码这种突变体s-GDH的基因以及这些s-GDH突变体的不同应用，特别是用于测定样品中葡萄糖浓度。
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公开(公告)号：US20090264618A1
公开(公告)日：2009-10-22
申请号：US12370886
申请日：2009-02-13
CPC分类号： G01N33/6845 , C07K1/1075 , C07K1/1077 , C07K14/001 , G01N33/5306 , G01N33/531 , G01N33/532 , G01N33/533 , G01N33/68
摘要： Disclosed herein are branched linkers of the formula: Zn-Y-Xm Wherein X, Y, Z, m, and n are as defined herein. The branched linkers are useful for producing conjugates that are used in diagnostic or therapeutic methods. Methods of producing the branched linkers are also described.
摘要翻译： 本文公开了下式的支链接头：Zn-Y-Xm，其中X，Y，Z，m和n如本文所定义。 支链接头可用于制备用于诊断或治疗方法的缀合物。 还描述了生产支化接头的方法。
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公开(公告)号：US20090246814A1
公开(公告)日：2009-10-01
申请号：US12330725
申请日：2008-12-09
CPC分类号： G01N33/723 , G01N2333/95
摘要： The present invention relates to a reagent for digestion of hemoglobin comprising a buffer, pepsin and a 1,3-dialkyl-imidazolium salt. It also discloses to the use of this reagent in a method for digesting hemoglobin, in a method for detecting HbA1c, and to a sampling tube for collection of a whole blood sample comprising said reagent for digesting hemoglobin.
摘要翻译： 本发明涉及一种用于消化血红蛋白的试剂，其包含缓冲液，胃蛋白酶和1,3-二烷基 - 咪唑鎓盐。 还公开了在用于消化血红蛋白的方法中使用该试剂，在检测HbA1c的方法中，以及用于收集包含用于消化血红蛋白的所述试剂的全血样品的采样管。
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公开(公告)号：US20080319182A1
公开(公告)日：2008-12-25
申请号：US12105556
申请日：2008-04-18
CPC分类号： C12N15/1006
摘要： Disclosed are water-soluble ionic liquids suitable for promoting adsorption of nucleic acids to a solid phase. The use thereof, particularly methods for the isolation of nucleic acids from an aqueous solution, as well as kits for performing those methods are disclosed.
摘要翻译： 公开了适用于促进核酸吸附到固相的水溶性离子液体。 公开了其用途，特别是用于从水溶液中分离核酸的方法，以及用于实施这些方法的试剂盒。
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公开(公告)号：US20070190530A1
公开(公告)日：2007-08-16
申请号：US10573215
申请日：2004-12-01
CPC分类号： C07H21/00 , C12Q1/6827 , C12Q2527/125 , C12Q2523/125
摘要： The invention is related to the detection of a methylated cytosine in a nucleic acid wherein guanidinium hydrogen sulfite is used for the preparation of a solution containing guanidinium ions and sulfite ions and subsequent modification of the nucleic acid. Thereby, a non-methylated cytosine is converted to uracil. The invention further discloses kits for performing the methods of the invention.
摘要翻译： 本发明涉及检测核酸中的甲基化胞嘧啶，其中将亚硫酸氢胍用于制备含有胍离子和亚硫酸根离子的溶液，随后改变核酸。 由此，将非甲基化胞嘧啶转化为尿嘧啶。 本发明还公开了用于执行本发明方法的套件。
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