公开(公告)号：US11124829B2

公开(公告)日：2021-09-21

申请号：US16626452

申请日：2019-06-07

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/68 ,  G03F7/16 ,  C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/00 ,  G03F7/004

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20210139975A1

公开(公告)日：2021-05-13

申请号：US17139834

申请日：2020-12-31

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US09994687B2

公开(公告)日：2018-06-12

申请号：US14316478

申请日：2014-06-26

Applicant: Illumina, Inc.

Inventor： Lorenzo Berti ,  Andrew A. Brown ,  Wayne N. George

IPC: C08J7/12 ,  C12Q1/68 ,  C08F8/00 ,  C09D133/26 ,  B01J19/00 ,  C08F220/60

CPC classification number: C08J7/12 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  C08F8/00 ,  C08F2220/603 ,  C08J2335/00 ,  C09D133/26 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2527/125 ,  C12Q2565/537 ,  C12Q2565/629

Abstract: Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.

公开(公告)号：US12202950B2

公开(公告)日：2025-01-21

申请号：US18190685

申请日：2023-03-27

Applicant: Illumina, Inc.

Inventor： Lorenzo Berti ,  Andrew A. Brown ,  Wayne N. George

IPC: C08J7/12 ,  B01J19/00 ,  C08F8/00 ,  C09D133/26 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q1/6876 ,  C08F220/60

Abstract: Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.

公开(公告)号：US20240352519A1

公开(公告)日：2024-10-24

申请号：US18758482

申请日：2024-06-28

Applicant: Illumina, Inc.

Inventor： Wayne N. George ,  Andrew A. Brown ,  Daniel Bratton ,  Hongji Ren ,  Ryan Christopher Smith

IPC: C12Q1/6874 ,  B05D3/06 ,  C09D133/26 ,  C09D135/00 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  B05D3/06 ,  B05D3/065 ,  C09D133/26 ,  C09D135/00 ,  C12Q1/6806 ,  Y10T428/24802 ,  Y10T428/24917 ,  Y10T428/24926 ,  Y10T428/2998 ,  Y10T428/31536 ,  Y10T428/31935

Abstract: The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein.

公开(公告)号：US12013330B2

公开(公告)日：2024-06-18

申请号：US16515849

申请日：2019-07-18

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Timothy J. Merkel ,  Wayne N. George ,  Andrew A. Brown ,  Audrey Zak ,  Gianluca Andrea Artioli ,  Julia Morrison ,  Nikolai Romanov ,  Lorenzo Berti ,  Graham Boud

IPC: G03F7/00 ,  C08L63/00 ,  C08L83/12 ,  G01N15/1434

CPC classification number: G01N15/1436 ,  C08L63/00 ,  C08L83/12 ,  G03F7/0002

Abstract: An example of a resin composition includes an epoxy resin matrix, a free radical photoinitiator selected from the group consisting of 2-ethyl-9,10-dimethoxyanthracene, 2,2-dimethoxy-2-phenylacetophenone, 2-ethoxy-2-phenylacetophenone, and a phosphine oxide, and a photoacid generator. When cured, the resin composition has low or no autofluorescence when exposed to blue excitation wavelengths ranging from about 380 nm to about 480 nm or green excitation wavelengths ranging from about 510 nm to about 560 nm.

公开(公告)号：US20230332224A1

公开(公告)日：2023-10-19

申请号：US18317865

申请日：2023-05-15

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

CPC classification number: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0002

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US11787930B2

公开(公告)日：2023-10-17

申请号：US16721451

申请日：2019-12-19

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Ludovic Vincent ,  Andrew A. Brown ,  Mathieu Lessard-Viger

IPC: C08L33/08 ,  C12Q1/6869 ,  C12Q1/6853 ,  C08L33/26

CPC classification number: C08L33/08 ,  C08L33/26 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

公开(公告)号：US20220088834A1

公开(公告)日：2022-03-24

申请号：US17541089

申请日：2021-12-02

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Alexandre Richez ,  Andrew A. Brown ,  Julia Morrison ,  Wayne N. George ,  Timothy J. Merkel ,  Audrey Rose Zak

IPC: B29C33/38 ,  C09D133/26 ,  B29C59/02 ,  G03F7/00 ,  B29C33/40

Abstract: An imprinting apparatus includes a silicon master having a plurality of nanofeatures defined therein. An anti-stick layer coats the silicon master, the anti-stick layer including a molecule having a cyclosiloxane with at least one silane functional group. A method includes forming a master template by: depositing a formulation on a silicon master including a plurality of nanofeatures defined therein, the formulation including a solvent and a molecule having a cyclosiloxane with at least one silane functional group; and curing the formulation, thereby forming an anti-stick layer on the silicon master, the anti-stick layer including the molecule. The method further includes depositing a silicon-based working stamp material on the anti-stick layer of the master template; curing the silicon-based working stamp material to form a working stamp including a negative replica of the plurality of nanofeatures; and releasing the working stamp from the master template.

公开(公告)号：US11173466B2

公开(公告)日：2021-11-16

申请号：US16118307

申请日：2018-08-30

Applicant: Illumina, Inc.

Inventor： Steven M. Barnard ,  M. Shane Bowen ,  Maria Candelaria Rogert Bacigalupo ,  Wayne N. George ,  Andrew A. Brown ,  James Tsay

IPC: B01J19/00 ,  C12N15/10 ,  C12Q1/6837

Abstract: An example method includes contacting a substrate coated with a sol-gel material with a stamp that includes a plurality of protruding features. While contacting the coated sol-gel material with the stamp, the example method further includes curing the coated sol-gel material so as to form a patterned sol-gel layer that includes a plurality of wells. The stamp is separated from the patterned sol-gel layer.