公开(公告)号：US10408823B2

公开(公告)日：2019-09-10

申请号：US15311309

申请日：2015-05-15

Applicant: MESO SCALE TECHNOLOGIES, LLC.

Inventor： Anahit Aghvanyan ,  Eli N. Glezer ,  John Kenten ,  George Sigal ,  Martin Stengelin ,  David Routenberg

IPC: C12Q1/6832 ,  C12Q1/6837 ,  G01N33/543 ,  C12Q1/6844

Abstract: The present invention is directed to methods for improving assay specificity and performance in binding assays.

公开(公告)号：US20190257823A1

公开(公告)日：2019-08-22

申请号：US16401513

申请日：2019-05-02

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  Kent Johnson ,  Michael Tsionsky ,  John H. Kenten ,  Jeff D. Debad ,  Robert M. Umek ,  Paula Denney Eason ,  Hans Biebuyck ,  Jacob N. Wohlstadter ,  James Wilbur ,  George Sigal ,  Niranjan Y. Sardesai

IPC: G01N33/53 ,  G01N33/68 ,  G01N33/573 ,  G01N33/569 ,  C12Q1/48 ,  G01N33/543

Abstract: Multiplexed test measurements are conducted using an assay module having a plurality of assay domains. In preferred embodiments, these measurements are conducted in assay modules having integrated electrodes with a reader apparatus adapted to receive assay modules, induce luminescence, preferably electrode induced luminescence, in the wells or assay regions of the assay modules and measure the induced luminescence.

公开(公告)号：US20190083976A1

公开(公告)日：2019-03-21

申请号：US16210165

申请日：2018-12-05

Applicant: Meso Scale Technologies, LLC

Inventor： Eli N. Glezer ,  Sudeep Kumar ,  Pankaj Oberoi ,  George Sigal ,  Michael Tsionsky

IPC: B01L3/00 ,  C12Q1/6804 ,  G01N33/68 ,  G01N33/543 ,  C12Q1/6876 ,  C12Q1/6816

Abstract: The invention relates to methods for conducting solid-phase binding assays. One example is an assay method having improved analyte specificity where specificity is limited by the presence of non-specific binding interactions.

公开(公告)号：US20180238882A1

公开(公告)日：2018-08-23

申请号：US15886020

申请日：2018-02-01

Applicant: Meso Scale Technologies, LLC.

Inventor： Michael Tsionsky ,  Eli N. Glezer ,  Selen Stromgren ,  George Sigal ,  Jonathan K. Leland ,  Mark A. Billadeau ,  Svetlana Leytner ,  Mark Martin ,  Larry Helms

IPC: G01N33/573 ,  G01N33/58 ,  C12Q1/48 ,  G01N21/76 ,  G01N33/53 ,  G01N33/543

Abstract: The invention relates to improved electrochemiluminescence assay methods for phosphorylated peptides or proteins employing phospho-specific antibodies and buffer compositions that are substantially free of inorganic phosphate.

公开(公告)号：US20180088108A1

公开(公告)日：2018-03-29

申请号：US15813411

申请日：2017-11-15

Applicant: Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  Anu Mathew

IPC: G01N33/543 ,  G01N33/574

CPC classification number: G01N33/543 ,  G01N33/57438 ,  G01N2800/52

Abstract: The present invention relates to methods of diagnosing a kidney disorder in a patient, as well as methods of monitoring the progression of a kidney disorder and/or methods of monitoring a treatment protocol of a therapeutic agent or a therapeutic regimen. The invention also relates to assay methods used in connection with the diagnostic methods described herein.

公开(公告)号：US09921166B2

公开(公告)日：2018-03-20

申请号：US14478197

申请日：2014-09-05

Applicant: Meso Scale Technologies, LLC

Inventor： Eli N. Glezer ,  Jonathan K. Leland ,  Mark A. Billadeau ,  Joseph M. Leginus ,  Bandele Jeffrey-Coker ,  Jeff D. Debad ,  Koustubh A. Phalnikar ,  Sriram Jambunathan

IPC: G01N21/76 ,  B01L3/00 ,  B01L9/00 ,  C12Q1/00 ,  G01N33/543 ,  G01N27/30 ,  B01J19/00 ,  B33Y80/00 ,  C40B40/10

CPC classification number: G01N21/76 ,  B01J19/0046 ,  B01J2219/00653 ,  B01J2219/00725 ,  B01J2219/0074 ,  B01L3/5027 ,  B01L3/502715 ,  B01L3/5029 ,  B01L9/527 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/0887 ,  B01L2400/049 ,  B01L2400/0688 ,  B33Y80/00 ,  C12Q1/001 ,  C40B40/10 ,  G01N27/30 ,  G01N33/5438 ,  G01N2458/30 ,  Y10T436/25 ,  Y10T436/25375 ,  Y10T436/255

Abstract: Assay modules, preferably assay cartridges, are described as are reader apparatuses which may be used to control aspects of module operation. The modules preferably comprise a detection chamber with integrated electrodes that may be used for carrying out electrode induced luminescence measurements. Methods are described for immobilizing assay reagents in a controlled fashion on these electrodes and other surfaces. Assay modules and cartridges are also described that have a detection chamber, preferably having integrated electrodes, and other fluidic components which may include sample chambers, waste chambers, conduits, vents, bubble traps, reagent chambers, dry reagent pill zones and the like. In certain preferred embodiments, these modules are adapted to receive and analyze a sample collected on an applicator stick.

公开(公告)号：US20180074082A1

公开(公告)日：2018-03-15

申请号：US15564581

申请日：2016-04-06

Applicant: Eli N. GLEZER ,  Carl EDENS ,  Bandele JEFFREY-COKER ,  Sandor KOVACS ,  Christopher FRYE ,  Meso Scale Technologies, LLC.

Inventor： Eli N. Glezer ,  Carl Edens ,  Bandele Jeffery-Coker ,  Sandor Kovacs ,  Christopher Frye

IPC: G01N35/00 ,  B01F11/00 ,  G01N35/10 ,  G01N33/543 ,  B06B1/16

Abstract: The present invention relates to a system for performing assays on a solid phase to measure the level of analyte in a sample. Such a system may perform immunoassays using electrochemiluminescence (ECL) including a counterbalanced orbital shaking apparatus for assay consumables.

公开(公告)号：US09878323B2

公开(公告)日：2018-01-30

申请号：US13662553

申请日：2012-10-29

Applicant: MESO SCALE TECHNOLOGIES, LLC

Inventor： Eli N. Glezer ,  Bandele Jeffrey-Coker ,  Jeffery D. Debad ,  Sudeep M. Kumar ,  George B. Sigal ,  Gisbert Spieles ,  Michael Tsionsky ,  Michael Warnock

IPC: C12Q1/00 ,  B01L3/00 ,  G01N33/543

CPC classification number: B01L3/5025 ,  B01L3/5085 ,  B01L2200/12 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0829 ,  B01L2300/0851 ,  B01L2300/0858 ,  B01L2300/105 ,  G01N33/54366 ,  G01N33/54393 ,  Y10S436/809

Abstract: We describe assay modules (e.g., assay plates, cartridges, multi-well assay plates, reaction vessels, etc.), processes for their preparation, and method of their use for conducting assays. Reagents may be present in free form or supported on solid phases including the surfaces of compartments (e.g., chambers, channels, flow cells, wells, etc.) in the assay modules or the surface of colloids, beads, or other particulate supports. In particular, dry reagents can be incorporated into the compartments of these assay modules and reconstituted prior to their use in accordance with the assay methods. A desiccant material may be used to maintain and stabilize these reagents in a dry state.

公开(公告)号：US20170242012A1

公开(公告)日：2017-08-24

申请号：US15488743

申请日：2017-04-17

Applicant: Meso Scale Technologies, LLC.

Inventor： Simone Barbero ,  Eli N. Glezer ,  Anu Mathew

IPC: G01N33/574 ,  A61K31/5377

CPC classification number: G01N33/57415 ,  A61K31/5377 ,  C12Q1/42 ,  C12Q1/485 ,  G01N2333/912 ,  G01N2333/916 ,  G01N2800/52

Abstract: The present invention relates to methods of diagnosing breast cancer in a patient, as well as methods of monitoring the progression of breast cancer and/or methods of monitoring a treatment protocol of a therapeutic agent or a therapeutic regimen. The invention also relates to assay methods used in connection with the diagnostic methods described herein.

公开(公告)号：US20140378347A1

公开(公告)日：2014-12-25

申请号：US14478221

申请日：2014-09-05

Applicant: Meso Scale Technologies, LLC

Inventor： Jeff D. Debad ,  Eli N. Glezer ,  Sudeep M. Kumar

IPC: G01N33/68

CPC classification number: G01N33/6893 ,  G01N33/6863 ,  G01N2333/523 ,  G01N2333/525 ,  G01N2333/70578 ,  G01N2800/065 ,  G01N2800/52

Abstract: Disclosed are methods for conducting diagnostic tests for the detection of the inflammatory bowel diseases, such as Crohn's disease and ulcerative colitis. Also described are methods for monitoring a patient by administering tests of the present invention. Also described are methods for monitoring patient's treatment by administering tests of the present invention. Also described are methods for evaluating the effectiveness of a drug or a drug candidate by administering tests of the present invention to samples from patients, animal models, and cell cultures treated with a drug or a drug candidate. Also disclosed are methods for determining the usefulness of analytes, e.g. cytokines, for acting as diagnostic and monitoring markers for inflammatory bowel disease in the various methods of the invention.

Abstract translation: 公开了用于进行诸如克罗恩病和溃疡性结肠炎的炎症性肠病的检测的诊断测试的方法。 还描述了通过管理本发明的测试来监测患者的方法。 还描述了通过管理本发明的测试来监测患者治疗的方法。 还描述了通过将本发明的试验施用于用药物或候选药物治疗的患者，动物模型和细胞培养物的样品来评价药物或药物候选物的有效性的方法。 还公开了用于确定分析物的有用性的方法，例如， 用作本发明各种方法中用于炎性肠病的诊断和监测标志物的细胞因子。