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公开(公告)号：US5779977A
公开(公告)日：1998-07-14
申请号：US827353
申请日：1997-03-26
IPC分类号： C12N15/09 , B01J19/00 , B01L7/00 , C12M1/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N35/00 , G01N15/06
CPC分类号： B01L7/525 , B01J19/0046 , B01L7/52 , B01L9/065 , B01J2219/00495 , B01J2219/00722 , B01L2300/1883 , C12Q1/686 , C40B40/06 , C40B60/14 , G01N2035/00237 , Y10T436/116664
摘要： Apparatus and method for performing a nucleic acid amplification reaction and preferably a polymerase chain reaction (PCR) in a reaction mixture in at least one capillary tube. Several different embodiments are disclosed. One embodiment cycles a sample through a capillary tube loop passing through two thermostatted fluid baths. Another embodiment has the capillary tube routed alternatingly between two heat exchangers to that the sample makes only one pass through the tube. Other embodiments maintain the heat exchangers stationary and translate the samples between them. Still further embodiments maintain the samples stationary and either automatically translate or rotate the heat exchangers past the samples contained within the capillary tubes to perform the thermal cycles necessary for the amplification reaction.
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公开(公告)号：US5710381A
公开(公告)日：1998-01-20
申请号：US198079
申请日：1994-03-01
申请人： John Girdner Atwood , Albert Carmelo Mossa , Lisa May Goven , Fenton Williams , Timothy M. Woudenberg , Marcel Margulies , Robert P. Ragusa , Richard Leath , Clive Miles
发明人： John Girdner Atwood , Albert Carmelo Mossa , Lisa May Goven , Fenton Williams , Timothy M. Woudenberg , Marcel Margulies , Robert P. Ragusa , Richard Leath , Clive Miles
IPC分类号： C12M1/00 , B01L3/00 , B01L3/14 , B01L7/00 , B01L9/00 , C12M1/02 , C12M1/34 , C12M1/36 , C12M1/38 , C12M1/40 , C12N15/00 , C12N15/09 , C12P19/34 , C12Q20060101 , C12Q1/00 , C12Q1/24 , C12Q1/68 , G01N35/00 , G05B13/00 , G05B15/00 , G05D23/00 , G05D23/19
CPC分类号： G05D23/1935 , B01L3/50851 , B01L3/50853 , B01L7/00 , B01L7/52 , G05D23/1917 , G05D23/1919 , B01L2200/147 , B01L2300/042 , B01L2300/046 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , B01L2300/185 , B01L3/5082
摘要： A two-piece plastic holder for loosely holding microliter sample tubes of a preselected design, each tube having a cylindrically shaped upper section open at its top end, a closed, tapered lower section extending downwardly therefrom, and a circumferential shoulder extending outwardly from the upper section at a position on the upper section spaced from the open end thereof. The holder includes a one-piece tray member and a one-piece retainer. The one-piece tray member including a flat, horizontal plate section containing holes in a rectangular array, a first vertical tray sidewall section around the plate extending upwardly to a height greater than the height of a tube resting in one of the holes, and a second vertical tray sidewall section around the plate extending downwardly approximately to the bottom of the upper section of a tube resting in one of the holes. The one-piece retainer is releasably engageable inside the tray over any sample tubes resting in the tray. The retainer comprises a flat, horizontal plate section containing holes in a rectangular array, and a vertical retainer sidewall section around the retainer plate section extending upwardly from the plate. When the retainer is engaged inside the tray, the retainer plate section lies slightly above the shoulder of a tube resting in the tray and the first tray sidewall section is about as high as the retainer sidewall section, so that tubes resting in the tray are retained loosely both vertically and laterally.
摘要翻译： 一种用于松散地保持预选设计的微升样品管的两件式塑料保持器，每个管具有在其顶端开口的圆柱形上部，从其向下延伸的封闭的锥形下部，以及从上部向外延伸的周向肩部 在上部的与其开口端间隔开的位置。 保持器包括一体式托盘构件和一体式保持器。 一体式托盘构件包括平坦的水平板部分，其包含矩形阵列的孔，围绕板延伸的第一垂直托盘侧壁部分向上延伸到高于搁置在其中一个孔中的管的高度的高度，以及 围绕板的第二垂直托盘侧壁部分大致向下延伸到搁置在其中一个孔中的管的上部的底部。 单件保持器可释放地接合在托盘内部的任何搁置在托盘中的样品管上。 保持器包括平坦的水平板部分，其包含矩形阵列的孔，以及围绕从板延伸的保持板部分的垂直保持器侧壁部分。 当保持器接合在托盘内部时，保持器板部分位于搁置在托盘中的管的稍微上方，并且第一托盘侧壁部分与保持器侧壁部分一样高，使得搁置在托盘中的管被保持 在垂直方向和横向均松动。
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33.发明授权Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control 失效热循环仪用于自动执行聚合酶链反应，并进行温度控制
公开(公告)号：US5475610A
公开(公告)日：1995-12-12
申请号：US871264
申请日：1992-04-20
申请人： John G. Atwood , Albert C. Mossa , Lisa M. Goven , Fenton Williams , Timothy M. Woudenberg , Marcel Margoulies , Robert P. Ragusa , Richard Leath , Clive Miles
发明人： John G. Atwood , Albert C. Mossa , Lisa M. Goven , Fenton Williams , Timothy M. Woudenberg , Marcel Margoulies , Robert P. Ragusa , Richard Leath , Clive Miles
IPC分类号： C12M1/00 , B01L3/00 , B01L3/14 , B01L7/00 , B01L9/00 , C12M1/02 , C12M1/34 , C12M1/36 , C12M1/38 , C12M1/40 , C12N15/00 , C12N15/09 , C12P19/34 , C12Q20060101 , C12Q1/00 , C12Q1/24 , C12Q1/68 , G01N35/00 , G05B13/00 , G05B15/00 , G05D23/00 , G05D23/19 , G06G7/58
CPC分类号： G05D23/1935 , B01L3/50851 , B01L3/50853 , B01L7/00 , B01L7/52 , G05D23/1917 , G05D23/1919 , B01L2200/147 , B01L2300/042 , B01L2300/046 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , B01L2300/185 , B01L3/5082
摘要： An instrument for performing highly accurate PCR employing a sample block in microtiter tray format. The sample block has local balance and local symmetry. A three zone film heater controlled by a computer and ramp cooling solenoid valves also controlled by the computer for gating coolant flow through the block controls the block temperature. Constant bias cooling is used for small changes. Sample temperature is calculated instead of measured. A platen deforms plastic caps to apply a minimum acceptable threshold force for seating the tubes and thermally isolates them. A cover isolates the block. The control software includes diagnostics. An install program tests and characterizes the instrument. A new user interface is used. Disposable, multipiece plastic microtiter trays to give individual freedom to sample tubes are taught.
摘要翻译： 使用微量滴定盘格式的样品块进行高精度PCR的仪器。 样品块具有局部平衡和局部对称性。 由计算机控制的三区薄膜加热器也由计算机控制的斜坡冷却电磁阀用于选通通过块的冷却剂流控制块温度。 恒定偏压冷却用于小的变化。 计算样品温度而不是测量。 压板使塑料盖变形，以施加最小可接受的阈值力，用于安置管并将其热隔离。 盖子隔离块。 控制软件包括诊断。 安装程序测试和表征仪器。 使用新的用户界面。 教授了一次性的多片塑料微量滴定塔，以给予样品管的个体自由度。
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公开(公告)号：US09205468B2
公开(公告)日：2015-12-08
申请号：US13516146
申请日：2010-11-30
申请人： Jing Wang , Timothy M. Woudenberg
发明人： Jing Wang , Timothy M. Woudenberg
CPC分类号： B08B9/027 , B01L3/5027 , B01L99/00 , B01L2300/123 , B01L2400/0481 , B01L2400/0655 , F16K99/0001 , F16K99/0026 , F16K99/0059 , F16K2099/0074 , F16K2099/0078 , F16K2099/008 , F16K2099/0084
摘要： A method for rendering a microfluidic device suitable for reuse for nucleic acid analysis is provided. The method may include flowing a nucleic acid inactivating solution into a microfluidic channel of the device by pumping; and then flowing a wash solution into the channel by pumping, thereby displacing the nucleic acid inactivating solution from the channel, whereby any residual nucleic acid from a prior use of the device is inactivated.
摘要翻译： 提供了一种用于制备适于重新用于核酸分析的微流体装置的方法。 该方法可以包括通过泵送将核酸灭活溶液流动到装置的微流体通道中; 然后通过泵送将洗涤溶液流入通道，从而使来自通道的核酸灭活溶液移位，由此来自先前使用该装置的任何残留核酸被灭活。
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35.发明申请公开(公告)号：US20100112572A1
公开(公告)日：2010-05-06
申请号：US12507016
申请日：2009-07-21
申请人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen , Benjamin Schroeder , Paolo Vatta
发明人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen , Benjamin Schroeder , Paolo Vatta
IPC分类号： C12Q1/68
CPC分类号： G01N33/533 , C12Q1/6816 , G01N33/532 , G01N2035/00158 , C12Q2565/102 , C12Q2563/179
摘要： The teachings herein generally relates to probes comprising fabricated coded molecular tags for detecting analytes. The teachings also relate to compositions, methods, and kits for fabricating coded molecular tags comprising a multiplicity or reporter groups in an ordered pattern.
摘要翻译： 本文的教导通常涉及包含用于检测分析物的制造的编码分子标签的探针。 该教导还涉及用于制造编码分子标签的组合物，方法和试剂盒，其包含有序图案中的多个或报告基团。
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36.发明申请公开(公告)号：US20100105886A1
公开(公告)日：2010-04-29
申请号：US12507035
申请日：2009-07-21
申请人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell
发明人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell
IPC分类号： C07H21/04
CPC分类号： C12Q1/6816 , C12Q2565/1025 , C12Q2563/107
摘要： The present teachings generally relate to probes and probes sets for detecting analytes. The teachings also relates to compositions, methods, and kits for assembling probes comprising at least one coded molecular tag.
摘要翻译： 本教导通常涉及用于检测分析物的探针和探针组。 该教导还涉及用于组装包含至少一个编码分子标签的探针的组合物，方法和试剂盒。
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37.发明申请Thermal cycler for automatic performance of the polymerase chain reaction with close temperature control 审中-公开热循环仪用于自动执行聚合酶链反应，并进行温度控制公开(公告)号：US20090155765A1
公开(公告)日：2009-06-18
申请号：US12291066
申请日：2008-11-05
申请人： John Girdner Atwood , Albert Carmelo Mossa , Lisa May Goven , Fenton Williams , Timothy M. Woudenberg , Marcel Margulies , Robert P. Rahusa , Richard Leath , Clive Miles
发明人： John Girdner Atwood , Albert Carmelo Mossa , Lisa May Goven , Fenton Williams , Timothy M. Woudenberg , Marcel Margulies , Robert P. Rahusa , Richard Leath , Clive Miles
CPC分类号： B01L3/50851 , B01L3/50853 , B01L7/00 , B01L7/52 , B01L2200/147 , B01L2300/042 , B01L2300/046 , B01L2300/0829 , B01L2300/1822 , B01L2300/1827 , B01L2300/185 , G05D23/1917 , Y10S435/809
摘要： A thermal cycler for automatic performance of the polymerase chain reaction is provided. The thermal cycler comprises a heater control that provides close temperature control of the reaction.
摘要翻译： 提供了用于自动执行聚合酶链式反应的热循环仪。 热循环仪包括提供对反应的温度控制的加热器控制。
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公开(公告)号：US07198900B2
公开(公告)日：2007-04-03
申请号：US10652430
申请日：2003-08-29
申请人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
发明人： Timothy M. Woudenberg , Dar Bahatt , Muhammad A. Sharaf , Timothy Z. Liu , Serguei Ermakov , Charles R. Connell , Jens J. Hyldig-Nielsen
CPC分类号： C12Q1/6827 , C12Q1/6858 , C12Q2565/1025 , C12Q2563/185 , C12Q2535/131 , C12Q2563/107
摘要： The present invention generally relates to the detection of analytes, particularly biomolecules in samples. The invention also relates to compositions, methods, and kits for detecting the presence of analytes, typically in multiplex detection formats. The invention also relates to methods for determining the presence of at least one analyte in a sample, the methods employing employ single molecule detection techniques to individually detect at least one molecular complex or at least part of a molecular complex.
摘要翻译： 本发明一般涉及检测分析物，特别是样品中的生物分子。 本发明还涉及通常以多重检测形式检测分析物的存在的组合物，方法和试剂盒。 本发明还涉及用于确定样品中至少一种分析物的存在的方法，采用单分子检测技术单独检测至少一种分子复合物或至少部分分子复合物的方法。
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公开(公告)号：US6033880A
公开(公告)日：2000-03-07
申请号：US27989
申请日：1998-02-23
申请人： Lawrence A. Haff , Enrico Picozza , Will Bloch , Robert Ragusa , Joseph DiCesare , David Tracy , Paul Saviano , Timothy M. Woudenberg , Richard W. Noreiks
发明人： Lawrence A. Haff , Enrico Picozza , Will Bloch , Robert Ragusa , Joseph DiCesare , David Tracy , Paul Saviano , Timothy M. Woudenberg , Richard W. Noreiks
IPC分类号： C12N15/09 , B01J19/00 , B01L7/00 , C12M1/00 , C12Q1/68 , C40B40/06 , C40B60/14 , G01N35/00 , C12P19/34
CPC分类号： B01L7/525 , B01J19/0046 , B01L7/52 , B01L9/065 , B01J2219/00495 , B01J2219/00722 , B01L2300/1883 , C12Q1/686 , C40B40/06 , C40B60/14 , G01N2035/00237 , Y10T436/116664
摘要： Apparatus and method for performing a nucleic acid amplification reaction and preferably a polymerase chain reaction (PCR) in a reaction mixture in at least one capillary tube. Several different embodiments are disclosed. One embodiment cycles a sample through a capillary tube loop passing through two thermostatted fluid baths. Another embodiment has the capillary tube routed alternatingly between two heat exchangers to that the sample makes only one pass through the tube. Other embodiments maintain the heat exchangers stationary and translate the samples between them. Still further embodiments maintain the samples stationary and either automatically translate or rotate the heat exchangers past the samples contained within the capillary tubes to perform the thermal cycles necessary for the amplification reaction.
摘要翻译： 用于在至少一个毛细管中的反应混合物中进行核酸扩增反应和优选聚合酶链式反应（PCR）的装置和方法。 公开了几个不同的实施例。 一个实施例通过穿过两个恒温流体浴的毛细管回路循环样品。 另一个实施例中，毛细管在两个热交换器之间交替布置，使得样品仅穿过管中一次。 其他实施例保持热交换器平稳，并在它们之间平移样品。 另外的实施例保持样品静止，并且自动地将热交换器平移或旋转通过毛细管内包含的样品，以进行扩增反应所需的热循环。
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40.发明授权Fluorescence monitoring device with textured optical tube and method for reducing background fluorescence 失效具有纹理光管的荧光监测装置和降低背景荧光的方法
公开(公告)号：US5972716A
公开(公告)日：1999-10-26
申请号：US568606
申请日：1995-12-05
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68 , G01N21/03 , G01N21/64 , G01N21/75 , G01N21/78 , G01N33/50 , G01N33/58
CPC分类号： C12Q1/6818 , C12Q1/6851 , C12Q1/686 , G01N21/0303 , G01N21/6428 , G01N2021/6484
摘要： An improved fluorescence monitoring apparatus for measuring fluorescent emission from a sample in response to sample irradiation by an emission beam is disclosed. The apparatus employs a sample tube having surface roughness characteristics which substantially reduce background fluorescence emission due to contamination of the tube holder in the apparatus. Also disclosed is a method of reducing such background, by texturing of a sample tube to produce desired roughness characteristics.
摘要翻译： 公开了一种改进的荧光监测装置，用于响应于发射光束的样品照射而测量来自样品的荧光发射。 该装置采用具有表面粗糙度特性的样品管，其显着地减少由于装置中的管保持器的污染引起的背景荧光发射。 还公开了通过样品管的纹理来产生所需的粗糙度特性来减少这种背景的方法。
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