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43 条记录 (耗时 0.037 秒)

    Themostable Phytase Variants
    31.
    发明申请
    Themostable Phytase Variants 审中-公开
    稳定的植酸酶变体

    公开(公告)号：US20130195829A1

    公开(公告)日：2013-08-01

    申请号：US13636197

    申请日：2011-03-25

    申请人： Lars Koberoee Skov ,  Leonardo De Maria ,  Tomoko Matsui

    发明人： Lars Koberoee Skov ,  Leonardo De Maria ,  Tomoko Matsui

    IPC分类号： C12N9/96 ,  C12P7/14 ,  C12P21/00

    CPC分类号： C12N9/96 ,  A23K10/16 ,  A23L33/195 ,  A61K38/46 ,  C12N9/16 ,  C12N15/8243 ,  C12P7/00 ,  C12P7/14 ,  C12P21/00 ,  C12Y301/03008 ,  C12Y301/03026 ,  C12Y301/03072

    摘要： The present invention relates to a method for producing phytase variants which has at least 74% identity to a phytase derived from Citrobacter braakii and comprises at least two additional disulfide bonds as compared to this phytase. These phytase variants have modified, preferably improved, properties, such as thermostability, temperature profile, pH profile, specific activity, performance in animal feed, reduced protease sensitiliby, and/or an modified glycosylation pattern. The invention also relates to the variants produced, DNA encoding these phytases, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.

    摘要翻译： 本发明涉及一种生产植酸酶变体的方法，所述植酸酶变体与来自柠檬酸杆菌（Citrobacter braakii）的植酸酶具有至少74％的同一性，并且与该植酸酶相比包含至少两个另外的二硫键。 这些植酸酶变体具有修饰的，优选改进的性质，例如热稳定性，温度曲线，pH曲线，比活性，动物饲料中的性能，降低的蛋白酶敏感性和/或修饰的糖基化模式。 本发明还涉及所生产的变体，编码这些植酸酶的DNA，其生产方法及其用途，例如， 在动物饲料和动物饲料添加剂中。

    Phytase variants
    32.
    发明授权
    Phytase variants 有权
    植酸酶变体

    公开(公告)号：US07238378B2

    公开(公告)日：2007-07-03

    申请号：US10358960

    申请日：2003-02-05

    申请人： Tomoko Matsui ,  Claus Crone Fuglsang ,  Allan Svendsen ,  Shiro Fukuyama

    发明人： Tomoko Matsui ,  Claus Crone Fuglsang ,  Allan Svendsen ,  Shiro Fukuyama

    IPC分类号： A23L1/29 ,  A23K1/165 ,  A23K1/175 ,  A23J3/14 ,  A23J3/34 ,  C12N9/16 ,  C12N15/55

    CPC分类号： C12N9/16 ,  A23K10/14 ,  A23K20/189

    摘要： The present invention relates to phytase variants derived from a parent phytase which is homologous to a Peniophora lycii phytase and comprises at least one substitution in at least one of a number of positions corresponding to a position in the Peniophora lycii phytase. The variants are of improved properties, in particular of a higher specific activity and/or more thermostable than the parent phytase, e.g. of a Tm of up to 82° C. at pH 5.5, as determined by DSC, and/or of a doubled specific activity. The invention also relates to DNA encoding the phytase variants, methods of their production, as well as the use thereof, e.g. in animal feed and animal feed additives.

    摘要翻译： 本发明涉及衍生自母本植酸酶的植酸酶变体，所述植酸酶与青i i植酸酶同源，并且包括至少一个位于对应于Peniophora lycii植酸酶中的位置的位置中的至少一个取代。 这些变体具有改进的性质，特别是比母体植酸酶更高的比活性和/或更高的热稳定性。 的Tm达到82℃，通过DSC测定，pH为5.5，和/或具有双重比活性。 本发明还涉及编码植酸酶变体的DNA，其生产方法及其用途，例如， 在动物饲料和动物饲料添加剂中。

    Expression of Genes from Gram Negative Bacteria in Fungi
    38.
    发明申请
    Expression of Genes from Gram Negative Bacteria in Fungi 审中-公开
    革兰氏阴性菌在真菌中的表达

    公开(公告)号：US20100261259A1

    公开(公告)日：2010-10-14

    申请号：US12376372

    申请日：2007-08-03

    申请人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

    发明人： Tomoko Matsui ,  Pia Francke Johannesen ,  Shinobu Takagi ,  Thomas Agersten Poulsen ,  Noriko Tsutsumi ,  Zheng Liu ,  XiangYu Kong

    IPC分类号： C12N1/19 ,  C12N1/15 ,  C07H21/04

    CPC分类号： C12N9/16 ,  C07K2319/02 ,  C12N15/80 ,  C12P21/02

    摘要： The present invention provides a method for the recombinant expression of polypeptides originating from gram negative bacteria, in a fungal host suitable for industrial production. In a first aspect the present invention relates to a method for recombinant expression of a polypeptide from a gram negative bacterium in a fungal host cell, comprising the steps: i) providing a nucleic acid sequence encoding the polypeptide, said nucleic acid sequence comprising a first nucleic acid sequence encoding a fungal signal peptide and a second nucleic acid sequence encoding the polypeptide, having at least one modified codon, wherein the modification does not change the amino acid encoded by said codon and the nucleic acid sequence of said codon is different compared to the corresponding codon in the wild type nucleic acid sequence present in the said gram negative bacterium; ii) expressing the modified nucleic acid sequence in the fungal host.

    摘要翻译： 本发明提供了在适于工业生产的真菌宿主中重组来自革兰氏阴性细菌的多肽的方法。 在第一方面，本发明涉及在真菌宿主细胞中从革兰氏阴性细菌重组表达多肽的方法，其包括以下步骤：i）提供编码所述多肽的核酸序列，所述核酸序列包含第一 编码真菌信号肽的核酸序列和编码具有至少一个修饰密码子的多肽的第二核酸序列，其中所述修饰不改变由所述密码子编码的氨基酸，并且所述密码子的核酸序列与 存在于所述革兰氏阴性细菌中的野生型核酸序列中相应的密码子; ii）在真菌宿主中表达经修饰的核酸序列。

    HAFNIA PHYTASE VARIANTS
    39.
    发明申请
    HAFNIA PHYTASE VARIANTS 有权

    公开(公告)号：US20100083392A1

    公开(公告)日：2010-04-01

    申请号：US12568312

    申请日：2009-09-28

    申请人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    发明人： Soeren Flensted Lassen ,  Leonardo De Maria ,  Esben Peter Friis ,  Tomoko Matsui ,  Allan Noergaard ,  Lars Kobberoee Skov ,  Jesper Vind

    IPC分类号： A01K67/00 ,  C12N9/16 ,  C12P1/00 ,  C12P7/06 ,  C07H21/00 ,  C12N15/63 ,  C12N1/00 ,  C12P21/00 ,  A01H5/00 ,  A23L1/28 ,  A23K1/18 ,  A23K1/16 ,  C12G1/00 ,  C12C11/00 ,  C12C7/00

    CPC分类号： C12N9/16 ,  A23K20/189 ,  A23K50/30 ,  A23K50/75 ,  Y02E50/17

    摘要： The present invention relates to phytases having at least 76% identity to a phytase derived from Hafnia alvei and comprises at least one modification in the amino acid sequence thereof. These phytase variants have modified, preferably improved, properties, such as, reduced protease sensibility, preferably they exhibit improved properties in respect of thermal performance, such as heat-stability (temperature stability, thermostability), steam stability, pelleting stability and/or temperature profile; and/or protease stability, in particular pepsin stability, pH profile, specific activity, substrate specificity, performance in animal feed (such as an improved release and/or degradation of phytate), susceptibility to glycation, and/or glycosylation pattern. The invention also relates to DNA encoding these phytases, methods of their production, as well as the use thereof, e.g., in animal feed and animal feed additives.

    摘要翻译： 本发明涉及与衍生自哈夫单胞菌属植物的植酸酶具有至少76％同一性的植酸酶，并且包含其氨基酸序列中的至少一个修饰。 这些植酸酶变体具有改性的，优选改进的性质，例如降低的蛋白酶敏感性，优选它们在热性能方面表现出改进的性能，例如热稳定性（温度稳定性，热稳定性），蒸汽稳定性，造粒稳定性和/或温度 简介 和/或蛋白酶稳定性，特别是胃蛋白酶稳定性，pH曲线，比活性，底物特异性，动物饲料中的性能（如植酸盐的改善的释放和/或降解），对糖基化的敏感性和/或糖基化模式。 本发明还涉及编码这些植酸酶的DNA，其生产方法及其用途，例如在动物饲料和动物饲料添加剂中。