公开(公告)号：US20230086845A1

公开(公告)日：2023-03-23

申请号：US17992304

申请日：2022-11-22

申请人： Bio-Rad Laboratories, Inc.

发明人： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC分类号： C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

摘要： The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US11603556B2

公开(公告)日：2023-03-14

申请号：US17087952

申请日：2020-11-03

申请人： Bio-Rad Laboratories, Inc.

发明人： Ronald Lebofsky

IPC分类号： C12Q1/68 ,  C12Q1/6834 ,  C12Q1/6855

摘要： Methods and compositions for attaching cell-specific barcodes without formation of partitions is provided.

公开(公告)号：US20230015223A1

公开(公告)日：2023-01-19

申请号：US17951719

申请日：2022-09-23

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Austin Payne ,  Vishal Sharma ,  Kyle Gleason ,  Priyadarshini Gogoi ,  Karthik Ganesan ,  Brian Boniface ,  Will Chow

IPC分类号： G01N15/14 ,  G01N1/20 ,  G01N1/40 ,  G01N1/28 ,  C12M1/00 ,  B01L3/00 ,  G01N35/10 ,  G01N35/00 ,  G06V10/141 ,  G06V10/145 ,  G06V10/147 ,  G06V20/69

摘要： A system and method for isolating and analyzing single cells, including: a substrate having a broad surface; a set of wells defined at the broad surface of the substrate, and a set of channels, defined by the wall, that fluidly couple each well to at least one adjacent well in the set of wells; and fluid delivery module defining an inlet and comprising a plate, removably coupled to the substrate, the plate defining a recessed region fluidly connected to the inlet and facing the broad surface of the substrate, the fluid delivery module comprising a cell capture mode.

公开(公告)号：US11504719B2

公开(公告)日：2022-11-22

申请号：US16816817

申请日：2020-03-12

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Austin Payne

IPC分类号： B01L99/00 ,  B01L3/00 ,  G01N1/00

摘要： A system and method for receiving and delivering a fluid, the system comprising: a body configured to interface with an opening of a reservoir and defining: a protrusion defining a set position of the body relative to the reservoir; a wall extending from the protrusion; a receiving surface coupled to the wall and sloping from an apex to a nadir along a first direction, the receiving surface comprising a vent; and an outlet positioned closer to the nadir than the apex of the receiving surface and displaced from the vent, the outlet comprising an extension from the body, the extension configured to contact an interior wall of the reservoir, wherein the body comprises: a bubble-mitigating operation mode in which the receiving surface receives and transmits the fluid along the receiving surface, and a fluid-transmitting operation mode in which the body directs the fluid along the interior wall of the reservoir.

公开(公告)号：US11499183B2

公开(公告)日：2022-11-15

申请号：US16022500

申请日：2018-06-28

申请人： Bio-Rad Laboratories, Inc.

发明人： David P. Stumbo ,  George Carman ,  Steve Hobbs ,  Anthony J. Makarewicz, Jr. ,  Dmitri Simonian ,  David Glade ,  Joshua Oen ,  Denis Pristinski ,  John Dzenitis

IPC分类号： G01N15/14 ,  B01F3/08 ,  C12Q1/6848 ,  C12Q1/6844 ,  C12Q1/686 ,  B01L3/00 ,  C12Q1/6851 ,  B01F23/41

摘要： Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.

公开(公告)号：US11479816B2

公开(公告)日：2022-10-25

申请号：US16545611

申请日：2019-08-20

申请人： Bio-Rad Laboratories, Inc.

发明人： Ronald Lebofsky ,  Andrew Kohlway ,  Jennifer Chew ,  Zachary Burkett ,  Man Cheng

IPC分类号： C12Q1/6869 ,  G16B40/10 ,  G16B30/10

摘要： Comparison of common sequencing reads from sequencing based on partition-based barcoding can be used to improve sequencing results. Increased loading of barcodes per partition can also improve sequencing results.

公开(公告)号：US11435285B2

公开(公告)日：2022-09-06

申请号：US17199312

申请日：2021-03-11

申请人： Bio-Rad Laboratories, Inc.

发明人： Evan Thrush ,  Steven Swihart ,  William Strong ,  Trey Marlowe ,  Li Lu

IPC分类号： G01N21/31 ,  G01N21/3577 ,  G01N21/3563

摘要： Systems for protein quantitation using a Fabry-Perot interferometer. In one arrangement, a quantitation device includes an infrared source, a sample holder, and a Fabry-Perot interferometer positioned to receive infrared radiation from the source passing through a sample on the sample holder. A band pass optical filter sets the working range of the interferometer, and radiation exiting the interferometer falls on a detector that produces a signal indicating the intensity of the received radiation. A controller causes the interferometer to be tuned to a number of different resonance wavelengths and receives the intensity signals, for determination of an absorbance spectrum.

公开(公告)号：US20220251668A1

公开(公告)日：2022-08-11

申请号：US17728789

申请日：2022-04-25

申请人： Bio-Rad Laboratories, Inc.

发明人： Kalyan Handique ,  Priyadarshini Gogoi ,  Yi Zhou ,  Saedeh Sepehri ,  Christopher Siemer

IPC分类号： C12Q1/6886 ,  C12Q1/6841 ,  G01N33/574 ,  B01L3/00 ,  C12Q1/6834

摘要： A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.

公开(公告)号：US11377684B2

公开(公告)日：2022-07-05

申请号：US15973243

申请日：2018-05-07

申请人： Bio-Rad Laboratories, Inc.

发明人： Duc Do ,  Claudia Litterst ,  Dianna Maar

IPC分类号： C12Q1/68 ,  C12Q1/686 ,  C12Q1/6844 ,  G01N21/64

摘要： Digital assay system, including methods, apparatus, and compositions, for assay of one or more targets in a set of partitions containing a generic reporter of target amplification.

公开(公告)号：US20220195484A1

公开(公告)日：2022-06-23

申请号：US17686466

申请日：2022-03-04

申请人： BIO-RAD EUROPE GMBH ,  BIO-RAD LABORATORIES, INC.

发明人： CHRISTOPHE QUIRING ,  CHRISTINE FAVIER ,  PATRICE SARFATI ,  JEAN FRANCOIS MOUSCADET ,  RONALD LEBOFSKY ,  REBECCA DIEVART

IPC分类号： C12Q1/06 ,  C12Q1/04 ,  C12N9/34 ,  C12N9/42 ,  C12N9/40 ,  C12N9/38 ,  C12N9/26 ,  C12N9/48

摘要： Methods, compositions, and kits are provided for rapidly analyzing microbial growth and/or number in a plurality of water-in-oil emulsion droplets.