公开(公告)号：US20160319290A2

公开(公告)日：2016-11-03

申请号：US14787408

申请日：2013-04-28

Applicant: INSTITUTE OF BOTANY, THE CHINESE ACADEMY OF SCIENCES

Inventor： Liangsheng WANG ,  Hui DU ,  Qingyan SHU

IPC: C12N15/82 ,  C12N9/10

CPC classification number: C12N15/825 ,  C12N9/1007 ,  C12N15/8205 ,  C12Y201/01

Abstract: The present invention provides novel protein and gene related to flavonoid O-methyltransferase (FOMT) and their uses therefore. The said protein having an amino acid sequence shown in SEQ ID NO: 3, or an amino acid sequence having deletion, substitution or insertion of one or plural amino acids in said amino acid sequence. The said gene comprising the nucleotide sequence shown in SEQ ID NO: 1, or a gene which hybridizes with said gene under stringent conditions and encodes a protein, which has anthocyanin 3′—O-methyltransferase or 3′,5′—O-methyltrasnferase activity. The present invention also provides a method for obtaining the transgenic plant used the above-mentioned gene.

Abstract translation: 本发明提供与类黄酮O-甲基转移酶（FOMT）相关的新型蛋白质和基因及其用途。 所述蛋白质具有SEQ ID NO：3所示的氨基酸序列，或在所述氨基酸序列中具有一个或多个氨基酸的缺失，取代或插入的氨基酸序列。 所述基因包含SEQ ID NO：1所示的核苷酸序列，或在严格条件下与所述基因杂交并编码具有花青素3'-O-甲基转移酶或3'，5'-O-甲基酪氨酸激酶的蛋白质的基因 活动。 本发明还提供了获得使用上述基因的转基因植物的方法。

公开(公告)号：US20160264934A1

公开(公告)日：2016-09-15

申请号：US15067780

申请日：2016-03-11

Applicant: THE GENERAL HOSPITAL CORPORATION ,  The Regents of the University of California

Inventor： Cosmas GIALLOURAKIS ,  Alan C. MULLEN ,  Yi XING

IPC: C12N5/0735 ,  A01N1/02 ,  C12Q1/68 ,  G01N33/573 ,  C12N5/077 ,  C12N5/0793

CPC classification number: C12N5/0606 ,  A01N1/0226 ,  C12N5/0619 ,  C12N5/0657 ,  C12N2501/72 ,  C12N2506/02 ,  C12Q1/6888 ,  C12Q2600/158 ,  C12Y201/01 ,  C12Y201/01062 ,  G01N33/5308 ,  G01N33/573 ,  G01N2440/12

Abstract: The present invention generally relates to methods, assays and kits to maintain a human stem cell population in an undifferentiated state by inhibiting the expression or function of METTL3 and/or METTL4, and m6A fingerprint methods, assays, arrays and kits to assess the cell state of a human stem cell population by assessing m6A levels (e.g. m6A peak intensities) of a set of target genes disclosed herein to determine if the stem cell is in an undifferentiated or differentiated state.

Abstract translation: 本发明一般涉及通过抑制METTL3和/或METTL4的表达或功能以及m6A指纹方法，测定，阵列和试剂盒来维持未分化状态的人类干细胞群体以评估细胞状态的方法，测定和试剂盒 通过评估本文公开的一组靶基因的m6A水平（例如m6A峰强度）来确定干细胞是否处于未分化或分化状态。

公开(公告)号：US20140058056A1

公开(公告)日：2014-02-27

申请号：US13975678

申请日：2013-08-26

Applicant: Genomatica, Inc.

Inventor： Anthony P. Burgard ,  Robin E. Osterhout ,  Stephen J. Van Dien ,  Cara Ann Tracewell ,  Priti Pharkya ,  Stefan Andrae

IPC: C12P7/18

CPC classification number: C12P7/18 ,  C07D207/12 ,  C08G18/3206 ,  C08G63/06 ,  C08G63/12 ,  C08G63/66 ,  C08G69/26 ,  C12N9/0006 ,  C12N9/0028 ,  C12N9/1007 ,  C12N9/88 ,  C12P7/24 ,  C12P19/02 ,  C12Y101/01224 ,  C12Y101/01244 ,  C12Y101/02007 ,  C12Y105/0102 ,  C12Y201/01 ,  C12Y201/0109 ,  C12Y402/01 ,  Y02P20/52

Abstract: Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 1,4-butanediol (BDO). Also provided herein are methods for using such an organism to produce BDO.

Abstract translation: 本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。 这种还原当量可用于增加由微生物生物例如1,4-丁二醇（BDO）产生的有机化合物的产物产率。 本文还提供了使用这种生物体产生BDO的方法。

公开(公告)号：US20130104258A1

公开(公告)日：2013-04-25

申请号：US13806310

申请日：2011-06-16

Applicant: Thilo Hans Winzer ,  Tracy Carol Walker ,  Ian Alexander Graham

Inventor： Thilo Hans Winzer ,  Tracy Carol Walker ,  Ian Alexander Graham

IPC: C12P17/18 ,  C12N9/10

CPC classification number: C12P17/188 ,  C07K14/415 ,  C12N9/1007 ,  C12N15/8243 ,  C12Y201/01

Abstract: This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode methyltransferase polypeptides from a Papaver somniferum cultivar; and uses in the production of noscapine and identification of poppy cultivars that include the genes that comprise said nucleic acid molecules.

Abstract translation: 本公开涉及从罂粟种植体品种编码甲基转移酶多肽的核酸分子的分离和测序; 并且用于生产诺斯卡品和鉴定包含所述核酸分子的基因的罂粟品种。

公开(公告)号：US20240255509A1

公开(公告)日：2024-08-01

申请号：US18506086

申请日：2023-11-09

Applicant: Aelan Cell Technologies, Inc.

Inventor： Victoria LUNYAK ,  James Robert TOLLERVEY

IPC: G01N33/573 ,  C07K14/47 ,  C07K16/18 ,  C12N9/10 ,  C12P21/02 ,  C12P21/06 ,  G01N33/543 ,  G01N33/68

CPC classification number: G01N33/573 ,  C07K14/47 ,  C07K16/18 ,  C12N9/1007 ,  C12P21/02 ,  C12P21/06 ,  C12Y201/01043 ,  G01N33/54306 ,  G01N33/6896 ,  C12Y201/01 ,  G01N2800/2821 ,  G01N2800/50

Abstract: Provided herein are H1.0K180me2 antibodies, H1.0K180me2 proteins, and H1.0K180me2 peptides and methods of use for diagnostics and therapeutics. These H1.0K180me2 antibodies, H1.0K180me2 proteins, and H1.0K180me2 peptides may be used in the treatment of methylated H1.0-related diseases or conditions in an individual. These H1.0K180me2 antibodies, H1.0K180me2 proteins, and H1.0K180me2 peptides may also be used for the detection and quantification of a histone H1.0 protein or fragment thereof, comprising a dimethylated lysine at lysine residue 180 (H1.0K180me2); such compositions and methods are useful for detecting replicative senescence, DNA damage, genotoxic stress, radiation exposure, Alzheimer's disease, are useful for monitoring therapeutic regimens, patient stratification, drug screening, and may serve as a marker of biological aging in a system.

公开(公告)号：US20240240207A1

公开(公告)日：2024-07-18

申请号：US18415999

申请日：2024-01-18

Applicant: The General Hospital Corporation

Inventor： J. Keith Joung ,  James Angstman ,  Shengdar Tsai

IPC: C12N15/90 ,  C07K14/005 ,  C07K14/195 ,  C12N9/02 ,  C12N9/10 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/10 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85

CPC classification number: C12N15/907 ,  C07K14/005 ,  C07K14/195 ,  C12N9/0071 ,  C12N9/1007 ,  C12N9/16 ,  C12N9/22 ,  C12N9/96 ,  C12N15/01 ,  C12N15/102 ,  C12N15/1031 ,  C12N15/11 ,  C12N15/63 ,  C12N15/85 ,  C12Y301/21004 ,  C07K2319/00 ,  C07K2319/01 ,  C07K2319/80 ,  C12N2310/20 ,  C12N2710/00033 ,  C12N2770/00033 ,  C12N2800/80 ,  C12Y114/11 ,  C12Y201/01 ,  C12Y301/00

Abstract: Methods for increasing specificity of RNA-guided genome editing, e.g., editing using CRISPR/Cas9 systems.

公开(公告)号：US20230193401A1

公开(公告)日：2023-06-22

申请号：US18112239

申请日：2023-02-21

Applicant: Jumpcode Genomics, Inc.

Inventor： Keith Brown

IPC: C12Q1/6886 ,  A61K49/00 ,  C12Q1/6869 ,  G16H15/00 ,  C12Q1/6809 ,  C12Q1/6883 ,  A61K31/7072 ,  A61K31/7056 ,  A61K38/45 ,  C12Q1/6876

CPC classification number: C12Q1/6886 ,  A61K31/7056 ,  A61K31/7072 ,  A61K38/45 ,  A61K49/0054 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/6876 ,  C12Q1/6883 ,  C12Y201/01 ,  G16H15/00 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/178 ,  Y02A90/10

Abstract: Methods and compositions related to the use of Mobile Element Insertions and their adjacent genomic sequences. Methods using MEIs as markers for cellular proliferation, as targets for pharmaceuticals, as markers for tissue fingerprinting and in related methods and compositions are disclosed herein. Methods and compositions relate to the detection, treatment and ongoing monitoring of cell proliferation events, cancer, and deleterious effects of mobile elements in aging, and to the selection, use and monitoring of the success of treatment regimens to address these conditions.

公开(公告)号：US20180187201A1

公开(公告)日：2018-07-05

申请号：US15809527

申请日：2017-11-10

Applicant: Danisco US Inc.

Inventor： Miles C. Scotcher ,  Derek H. Wells

IPC: C12N15/74 ,  C12N9/10 ,  C12P7/06 ,  C12P5/00 ,  C12N15/70

CPC classification number: C12N15/74 ,  C12N9/1007 ,  C12N15/70 ,  C12P5/007 ,  C12P7/065 ,  C12Y201/01 ,  Y02E50/17

Abstract: The invention provides compositions and methods for clostridial bacteria that have been engineered to produce and/or to improve efficiency of production of industrial bioproducts.

公开(公告)号：US20180135029A1

公开(公告)日：2018-05-17

申请号：US15514120

申请日：2015-09-27

Applicant: Symrise AG

Inventor： Ludger Wessjohann ,  Anne-Kathrin Bauer ,  Jakob Ley ,  Torsten Geissier ,  Katrin Geissler

IPC: C12N9/10 ,  C12N9/02 ,  C12N15/64 ,  C12P17/06

CPC classification number: C12N9/1007 ,  C12N9/0071 ,  C12N15/64 ,  C12P17/06 ,  C12Y114/14001 ,  C12Y201/01

Abstract: A method is proposed for producing flavonoids, comprising the steps: (a) providing of a transgenic microorganism, containing (i) a first nucleic acid section (A), comprising or consisting of a gene coding for a CYP450 oxidase, (ii) a second nucleic acid section (B), comprising or consisting of a gene coding for a plant O-methyltransferase, and (b) adding of one or more flavanones to the transgenic microorganism, (c) the conversion of the substrate flavanones by the transgenic microorganism to the corresponding flavonoids, and optionally (d) isolating and purifying of the final products.

公开(公告)号：US09926568B2

公开(公告)日：2018-03-27

申请号：US14899846

申请日：2014-06-20

Applicant: Danisco US Inc.

Inventor： Miles C. Scotcher ,  Derek H. Wells

IPC: C12N15/70 ,  C12N15/74 ,  C12N9/10 ,  C12P5/00 ,  C12P7/06

CPC classification number: C12N15/74 ,  C12N9/1007 ,  C12N15/70 ,  C12P5/007 ,  C12P7/065 ,  C12Y201/01 ,  Y02E50/17

Abstract: The invention provides compositions and methods for clostridial bacteria that have been engineered to produce and/or to improve efficiency of production of industrial bioproducts.