公开(公告)号：US20200209231A1

公开(公告)日：2020-07-02

申请号：US16811789

申请日：2020-03-06

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543 ,  B01F11/00 ,  G01N33/561 ,  C12N15/10 ,  B03C5/00 ,  B03C1/30 ,  B03C1/28 ,  B03C1/02 ,  B01L3/00 ,  B01F13/00

Abstract: Methods are provided for separating magnetically responsive beads from a droplet in a droplet actuator. Droplet operations electrodes and a magnet are arranged in a droplet actuator to manipulate a bead-containing droplet and position it relative to a magnetic field region that attracts the magnetically responsive beads. The droplet operations electrodes are operated to control the droplet shape and transport it away from the magnetic field region to form a concentration of beads in the droplet. The continued transport of the droplet away from the magnetic field causes the concentration of beads to break away from the droplet to yield a small, concentrated bead-containing droplet immobilized by the magnet.

公开(公告)号：US10078078B2

公开(公告)日：2018-09-18

申请号：US15210634

申请日：2016-07-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N15/06 ,  G01N33/00 ,  G01N33/48 ,  G01N33/543 ,  B01F11/00 ,  B01F13/00 ,  B01L3/00 ,  B03C1/02 ,  B03C1/30 ,  B03C5/00 ,  G01N33/561 ,  B03C1/28 ,  C12N15/10 ,  B01L7/00 ,  G01N35/10

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

公开(公告)号：US09861986B2

公开(公告)日：2018-01-09

申请号：US14506265

申请日：2014-10-03

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack ,  Prasanna Thwar ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Alexander Shenderov ,  Dwayne J. Allen

IPC: B01L3/00 ,  G01N27/447 ,  B01L7/00 ,  G01N35/10 ,  G01N35/00

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/022 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0683 ,  G01N27/44704 ,  G01N35/0098 ,  G01N2035/1046 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.

公开(公告)号：US09511369B2

公开(公告)日：2016-12-06

申请号：US14248884

申请日：2014-04-09

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Alexander Shenderov ,  Zhishan Hua ,  Arjun Sudarsan

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0427 ,  Y10T29/49826

Abstract: The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.

Abstract translation: 本发明提供一种液滴致动器。 液滴致动器可以包括分离以形成间隙的基底基板和顶基板。 基底基板可以包括被配置用于在间隙中进行液滴操作的电极; 并且顶部衬底可以包括联接到非玻璃部分的玻璃衬底部分，其中非玻璃部分可以包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。

公开(公告)号：US20160320378A1

公开(公告)日：2016-11-03

申请号：US15210634

申请日：2016-07-14

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vamsee K. Pamula ,  Michael G. Pollack ,  Ramakrishna Sista ,  Arjun Sudarsan

IPC: G01N33/543 ,  C12N15/10 ,  B03C1/30 ,  B03C5/00 ,  B03C1/28 ,  G01N33/561 ,  B01L3/00

CPC classification number: G01N33/54333 ,  B01F11/0071 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502715 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/502792 ,  B01L7/52 ,  B01L7/525 ,  B01L2200/027 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/089 ,  B01L2300/1827 ,  B01L2400/0406 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0442 ,  B03C1/02 ,  B03C1/288 ,  B03C1/30 ,  B03C5/005 ,  B03C2201/18 ,  B03C2201/26 ,  C12N15/1013 ,  G01N33/561 ,  G01N2035/1046 ,  Y10T436/11 ,  Y10T436/25 ,  Y10T436/25625 ,  Y10T436/2575

Abstract: The invention relates to bead incubating and washing on a droplet actuator. Methods for incubating magnetically responsive beads that are labeled with primary antibody, a sample (i.e., analyte), and secondary reporter antibodies on a magnet, on and off a magnet, and completely off a magnet are provided. Also provided are methods for washing magnetically responsive beads using shape-assisted merging of droplets. Also provided are methods for shape-mediated splitting, transporting, and dispensing of a sample droplet that contains magnetically responsive beads. The apparatuses and methods of the invention provide for rapid time to result and optimum detection of an analyte in an immunoassay.

公开(公告)号：US20150075985A1

公开(公告)日：2015-03-19

申请号：US14541825

申请日：2014-11-14

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack ,  Vamsee K. Pamula ,  Vijay Srinivasan

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/502761 ,  B01F13/0071 ,  B01F13/0076 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/0647 ,  B01L2200/0668 ,  B01L2200/16 ,  B01L2300/0819 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2300/088 ,  B01L2300/089 ,  B01L2400/0427 ,  B01L2400/043 ,  G01N27/447

Abstract: The invention provides nonlimiting examples of structures for and methods of dispensing droplets in a droplet actuator. The droplet actuator structures and methods of the invention exhibit numerous advantages over droplet actuators of the prior art. In various embodiments, the structures and methods of the invention provide, among other things, improved efficiency, throughput, scalability, and/or droplet uniformity, as compared with existing droplet actuators. Further, in some embodiments, the droplet actuators provide configurations for improved methods of loading and/or unloading fluid and/or droplets. In yet other embodiments, the droplet actuators provide fluid loading configurations for loading numerous fluid reservoirs in a substantially simultaneous and/or substantially sequential manner.

Abstract translation: 本发明提供了用于在液滴致动器中分配液滴的结构的方法和方法的非限制性实例。 本发明的液滴致动器结构和方法与现有技术的液滴致动器相比表现出许多优点。 在各种实施例中，与现有的液滴致动器相比，本发明的结构和方法尤其提供了改进的效率，吞吐量，可扩展性和/或液滴均匀性。 此外，在一些实施例中，液滴致动器提供用于加载和/或卸载流体和/或液滴的改进方法的构造。 在其他实施例中，液滴致动器提供用于以基本上同时和/或基本顺序的方式装载多个流体储存器的流体加载构造。

公开(公告)号：US20150027889A1

公开(公告)日：2015-01-29

申请号：US14506265

申请日：2014-10-03

Applicant: ADVANCED LIQUID LOGIC, INC.

Inventor： Michael G. Pollack ,  Prasanna Thwar ,  Vamsee K. Pamula ,  Vijay Srinivasan ,  Allen E. Eckhardt ,  Alexander Shenderov ,  Dwayne J. Allen

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  B01L3/502761 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0673 ,  B01L2300/022 ,  B01L2300/023 ,  B01L2300/024 ,  B01L2300/0672 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0424 ,  B01L2400/0427 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0481 ,  B01L2400/0487 ,  B01L2400/049 ,  B01L2400/0683 ,  G01N27/44704 ,  G01N35/0098 ,  G01N2035/1046 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides droplet actuators and droplet actuator cassettes including reagent storage capabilities, as well as methods of making and using the droplet actuators and cassettes. The invention also provides continuous flow channel elements and techniques for using electrodes to manipulate droplets in flowing streams. The invention also discloses methods of separating compounds on a droplet actuator. Various other aspects of the invention are also disclosed.

Abstract translation: 本发明提供了包括试剂储存能力的液滴致动器和液滴致动器盒，以及制造和使用液滴致动器和盒的方法。 本发明还提供了用于使用电极在流动流中操纵液滴的连续流动通道元件和技术。 本发明还公开了在液滴致动器上分离化合物的方法。 还公开了本发明的各种其它方面。

公开(公告)号：US20140216932A1

公开(公告)日：2014-08-07

申请号：US14248884

申请日：2014-04-09

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Alexander Shenderov ,  Zhishan Hua ,  Arjun Sudarsan

IPC: B01L3/00

CPC classification number: B01L3/502792 ,  B01L2200/12 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0421 ,  B01L2400/0427 ,  Y10T29/49826

Abstract: The invention provides a droplet actuator. The droplet actuator may include a base substrate and a top substrate separated to form a gap. The base substrate may include electrodes configured for conducting droplet operations in the gap; and the top substrate may include a glass substrate portion coupled to a non-glass portion, where the non-glass portion may include one or more openings establishing a fluid path extending from an exterior of the droplet actuator and into the gap. The invention also provides related methods of manufacturing the droplet actuator, methods of using the droplet actuator, and methods of loading the droplet actuator.

Abstract translation: 本发明提供一种液滴致动器。 液滴致动器可以包括分离以形成间隙的基底基板和顶基板。 基底基板可以包括被配置用于在间隙中进行液滴操作的电极; 并且顶部衬底可以包括联接到非玻璃部分的玻璃衬底部分，其中非玻璃部分可以包括建立从液滴致动器的外部延伸到间隙中的流体路径的一个或多个开口。 本发明还提供了制造液滴致动器的相关方法，使用液滴致动器的方法以及装载液滴致动器的方法。

公开(公告)号：US20140014517A1

公开(公告)日：2014-01-16

申请号：US14027941

申请日：2013-09-16

Applicant: Advanced Liquid Logic, Inc.

Inventor： Vijay Srinivasan ,  Michael G. Pollack ,  Zhishan Hua ,  Arjun Sudarsan ,  Allen E. Eckhardt

IPC: G01N27/447

CPC classification number: G01N27/44743 ,  B01L3/0268 ,  B01L3/502761 ,  B01L3/502792 ,  B01L2200/0605 ,  B01L2200/061 ,  B01L2200/0647 ,  B01L2300/0645 ,  B01L2300/16 ,  B01L2400/0415 ,  B01L2400/0427

Abstract: Droplet actuator for conducting droplet operations, such as droplet transport and droplet dispensing, is provided. In one embodiment, the droplet actuator may include an electrode that is rotationally but not reflectively symmetrical.

Abstract translation: 提供用于进行液滴操作的液滴致动器，例如液滴传输和液滴分配。 在一个实施例中，液滴致动器可以包括旋转但不反射对称的电极。

公开(公告)号：US20130252262A1

公开(公告)日：2013-09-26

申请号：US13890715

申请日：2013-05-09

Applicant: ADVANCED LIQUID LOGIC INC.

Inventor： Vijay Srinivasan ,  Vamsee K. Pamula ,  Michael G. Pollack ,  Richard B. Fair

IPC: G01N27/72

CPC classification number: G01N27/72 ,  G01N27/745 ,  G01N33/54326

Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.

Abstract translation: 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。