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    Chimeric osteogenic factor containing proteins capable of increased nuclear localization and methods of use thereof
    43.
    发明授权
    Chimeric osteogenic factor containing proteins capable of increased nuclear localization and methods of use thereof 有权
    含有能够增加核定位的蛋白质的嵌合成骨因子及其使用方法

    公开(公告)号：US07781574B2

    公开(公告)日：2010-08-24

    申请号：US11602120

    申请日：2006-11-20

    申请人： Scott D. Boden ,  Sreedhara Sangadala

    发明人： Scott D. Boden ,  Sreedhara Sangadala

    IPC分类号： C07H21/04 ,  C12P21/04 ,  C12N5/00 ,  C12N1/20 ,  C12N15/00

    CPC分类号： C07K16/18 ,  A61K35/12 ,  A61K35/13 ,  A61K48/00 ,  A61K2121/00 ,  A61L27/3843 ,  A61L27/3856 ,  A61L27/3895 ,  C07K14/47 ,  C07K14/78 ,  C12N2799/022

    摘要： Compositions comprising osteogenic factors fused with membrane transduction domains of viral proteins are provided. Also provided are methods of expression and use of such compositions. Further, the methods of making such compositions are also provided. The methods involve transfecting the cells with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter and optionally a membrane transduction domain of a viral protein. Transfection may be accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. Methods for treating disc disease associated with trauma or disc degeneration are also described.

    摘要翻译： 提供包含与病毒蛋白的膜转导结构域融合的成骨因子的组合物。 还提供了这些组合物的表达和使用方法。 此外，还提供了制备这种组合物的方法。 所述方法包括用分离的核酸转染细胞，该分离的核酸包含编码LIM矿化蛋白的核苷酸序列，其可操作地连接于启动子和任选的病毒蛋白的膜转导结构域。 转染可以通过直接注射病毒或裸露DNA或非病毒载体如质粒在体内或体内完成。 还描述了与创伤或椎间盘退变相关的椎间盘疾病的治疗方法。

    Novel Bone Mineralization Proteins, DNA, Vectors, Expression Systems
    45.
    发明申请
    Novel Bone Mineralization Proteins, DNA, Vectors, Expression Systems 审中-公开
    新型骨矿化蛋白，DNA，载体，表达系统

    公开(公告)号：US20090247733A1

    公开(公告)日：2009-10-01

    申请号：US11857938

    申请日：2007-09-19

    申请人： Gregory A. Hair ,  Scott D. Boden

    发明人： Gregory A. Hair ,  Scott D. Boden

    IPC分类号： C07K14/47

    CPC分类号： C07K14/51 ,  A61K48/00 ,  C07K14/47 ,  C12N2799/021

    摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.

    摘要翻译： 本发明涉及编码LIM矿化蛋白或LMP的分离的核酸分子。 本发明还提供了包含编码LMP的核苷酸序列的载体以及包含这些载体的宿主细胞。 此外，本发明涉及通过用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞诱导骨形成的方法。 转染可以通过直接注射病毒或裸体质粒DNA离体或体内发生。 在一个具体实施方案中，本发明提供了一种通过用具有编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞来融合脊柱的方法，将转染的成骨前体细胞与基质混合并使基质与脊柱 。 最后，本发明涉及通过用本发明的载体稳定转染宿主细胞诱导全身骨形成的方法。

    Chimeric osteogenic factor containing proteins capable of increased nuclear localization and methods of use thereof
    47.
    发明申请
    Chimeric osteogenic factor containing proteins capable of increased nuclear localization and methods of use thereof 有权
    含有能够增加核定位的蛋白质的嵌合成骨因子及其使用方法

    公开(公告)号：US20080118526A1

    公开(公告)日：2008-05-22

    申请号：US11602120

    申请日：2006-11-20

    申请人： Scott D. Boden ,  Sreedhara Sangadala

    发明人： Scott D. Boden ,  Sreedhara Sangadala

    IPC分类号： A61K39/21 ,  C07H21/04 ,  C12N15/867 ,  C07H21/02 ,  C07K14/16

    CPC分类号： C07K16/18 ,  A61K35/12 ,  A61K35/13 ,  A61K48/00 ,  A61K2121/00 ,  A61L27/3843 ,  A61L27/3856 ,  A61L27/3895 ,  C07K14/47 ,  C07K14/78 ,  C12N2799/022

    摘要： Compositions comprising osteogenic factors fused with membrane transduction domains of viral proteins are provided. Also provided are methods of expression and use of such compositions. Further, the methods of making such compositions are also provided. The methods involve transfecting the cells with an isolated nucleic acid comprising a nucleotide sequence encoding a LIM mineralization protein operably linked to a promoter and optionally a membrane transduction domain of a viral protein. Transfection may be accomplished ex vivo or in vivo by direct injection of virus or naked DNA, or by a nonviral vector such as a plasmid. Methods for treating disc disease associated with trauma or disc degeneration are also described.

    摘要翻译： 提供包含与病毒蛋白的膜转导结构域融合的成骨因子的组合物。 还提供了这些组合物的表达和使用方法。 此外，还提供了制备这种组合物的方法。 所述方法包括用分离的核酸转染细胞，所述分离的核酸包含编码LIM矿化蛋白的核苷酸序列，其可操作地连接于启动子和任选的病毒蛋白的膜转导结构域。 转染可以通过直接注射病毒或裸露DNA或非病毒载体如质粒在体内或体内完成。 还描述了与创伤或椎间盘退变相关的椎间盘疾病的治疗方法。

    Bone mineralization proteins, DNA, vectors expression systems
    48.
    发明授权
    Bone mineralization proteins, DNA, vectors expression systems 有权
    骨矿化蛋白，DNA，载体表达系统

    公开(公告)号：US06858431B2

    公开(公告)日：2005-02-22

    申请号：US09986625

    申请日：2001-11-09

    申请人： Gregory A. Hair ,  Scott D. Boden

    发明人： Gregory A. Hair ,  Scott D. Boden

    IPC分类号： C12N15/09 ,  A61K31/711 ,  A61K38/00 ,  A61K48/00 ,  A61P19/00 ,  A61P43/00 ,  C07K14/47 ,  C07K16/18 ,  C12N1/19 ,  C12N1/21 ,  C12N5/00 ,  C12N15/12 ,  C12P21/08 ,  C12Q1/68 ,  C12N15/63 ,  C07H21/02 ,  C07H21/04 ,  C12N15/85 ,  C12N15/86 ,  C12N15/87

    CPC分类号： C07K14/51 ,  A61K48/00 ,  C07K14/47 ,  C12N2799/021

    摘要： The present invention is directed to isolated nucleic acid molecules that encode LIM mineralization protein, or LMP. The invention further provides vectors comprising nucleotide sequences that encode LMP, as well as host cells comprising those vectors. Moreover, the present invention relates to methods of inducing bone formation by transfecting osteogenic precursor cells with an isolated nucleic acid molecule comprising a nucleotide sequence encoding LIM mineralization protein. The transfection may occur ex vivo or in vivo by direct injection of virus or naked plasmid DNA. In a particular embodiment, the invention provides a method of fusing a spine by transfecting osteogenic precursor cells with an isolated nucleic acid molecule having a nucleotide sequence encoding LIM mineralization protein, admixing the transfected osteogenic precursor cells with a matrix and contacting the matrix with the spine. Finally, the invention relates to methods for inducing systemic bone formation by stable transfection of host cells with the vectors of the invention.

    摘要翻译： 本发明涉及编码LIM矿化蛋白或LMP的分离的核酸分子。 本发明还提供了包含编码LMP的核苷酸序列的载体以及包含这些载体的宿主细胞。 此外，本发明涉及通过用包含编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞诱导骨形成的方法。 转染可以通过直接注射病毒或裸体质粒DNA离体或体内发生。 在一个具体实施方案中，本发明提供了一种通过用具有编码LIM矿化蛋白的核苷酸序列的分离的核酸分子转染成骨前体细胞来融合脊柱的方法，将转染的成骨前体细胞与基质混合并使基质与脊柱 。 最后，本发明涉及通过用本发明的载体稳定转染宿主细胞诱导全身骨形成的方法。