公开(公告)号：US20100233301A1

公开(公告)日：2010-09-16

申请号：US12713959

申请日：2010-02-26

Applicant: Jing Cheng ,  Chia-Wen Chen ,  Sleven Francis Schnittger ,  Ming Lu

Inventor： Jing Cheng ,  Chia-Wen Chen ,  Sleven Francis Schnittger ,  Ming Lu

IPC: A61K36/254 ,  A61K36/00 ,  A61K36/16 ,  A61K36/752

CPC classification number: A61K36/725 ,  A61K8/97 ,  A61K8/99 ,  A61K36/06 ,  A61K36/068 ,  A61K36/074 ,  A61K36/076 ,  A61K36/10 ,  A61K36/16 ,  A61K36/185 ,  A61K36/23 ,  A61K36/235 ,  A61K36/236 ,  A61K36/258 ,  A61K36/28 ,  A61K36/41 ,  A61K36/535 ,  A61K36/539 ,  A61K36/65 ,  A61K36/69 ,  A61K36/704 ,  A61K36/752 ,  A61K36/756 ,  A61K36/804 ,  A61K36/8998 ,  A61K2800/85 ,  A61Q19/00

Abstract: The present invention relates to topical compositions containing fermented extracts of Traditional Chinese Medicinal (TCM) ingredients for improving the appearance and skin condition of the user. The topical compositions of the present invention are tailored for different users of different skin compositions according to TCM principles. The fermented TCM extracts are characterized by reduced odor and/or color in comparison with unfermented TCM extracts and are therefore more suitable for use in cosmetic products.

Abstract translation: 本发明涉及含有用于改善使用者的外观和皮肤状况的中药（TCM）成分的发酵提取物的局部组合物。 根据TCM原理，本发明的局部组合物针对不同皮肤组合物的不同使用者而定制。 与未发酵的TCM提取物相比，发酵的TCM提取物的特征在于减少气味和/或颜色，因此更适合用于化妆品。

公开(公告)号：US20100151448A1

公开(公告)日：2010-06-17

申请号：US11661069

申请日：2004-11-22

Applicant: Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

Inventor： Zhiwei Zhang ,  Can Wang ,  Lingxiang Zhu ,  Qiong Zhang ,  Jing Cheng

IPC: C12Q1/68 ,  C07H21/04 ,  C12P19/34

CPC classification number: C12Q1/686 ,  C12Q2531/107 ,  C12Q2525/155 ,  C12Q2525/15

Abstract: The present invention discloses an asymmetric PCR amplification method, its special primer and application, aims to provide a simple, effective PCR amplification for preparation of single-stranded product. The asymmetric PCR primer of the invention comprises some PCR primer pairs, in which an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one primer. The asymmetric PCR amplification provided includes the steps: 1) preparative denaturing; 2) repetitiously denaturing, primers annealing, extending cycles as the first stage of PCR amplification; 3) repetitiously denaturing, primer extending cycles as the second stage of PCR amplification, wherein an unrelated nucleic acids sequence to target sequence to be detected is added onto 5′-terminal of one PCR primer of each pair in extension. With the asymmetric PCR amplification of the invention, high throughput of single-stranded products can be obtained, single PCR amplification or multiple PCR amplification can be carried out. And the method can be widely used in detection of nucleic acids.

Abstract translation: 本发明公开了一种不对称PCR扩增方法及其特异性引物及其应用，旨在为单链产物的制备提供简单有效的PCR扩增。 本发明的不对称PCR引物包含一些PCR引物对，其中将待检测的靶序列的不相关核酸序列添加到一个引物的5'末端。 提供的不对称PCR扩增包括以下步骤：1）制备型变性; 2）重复变性，引物退火，延伸循环作为PCR扩增的第一阶段; 3）重复变性，引物延伸循环作为PCR扩增的第二阶段，其中将待检测的靶序列的不相关核酸序列添加到每对扩增中的一个PCR引物的5'末端。 利用本发明的不对称PCR扩增，可以获得高通量的单链产物，可进行单PCR扩增或多重PCR扩增。 该方法可广泛用于检测核酸。

公开(公告)号：US20090322309A1

公开(公告)日：2009-12-31

申请号：US12424955

申请日：2009-04-16

Applicant: Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

Inventor： Jing Zhu ,  Zhongyao Yu ,  Xueling Quan ,  Guangxin Xiang ,  Yuming Hu ,  Wanli Xing ,  Jing Cheng

IPC: G01N27/00 ,  H05K1/16

CPC classification number: G01N33/4836

Abstract: Among others things, techniques, systems, and apparatus are disclosed for recording electrophysiological signals. In one aspect, a microelectrode sensing device includes a printed circuit board (PCB), a chip unit electrically connected to the PCB, and a cell culture chamber positioned over the chip unit and sealed to the PCB with the chip unit between the PCB and the cell culture chamber. The chip unit includes a substrate; a conductive layer positioned over the substrate that includes one or more recording electrodes; an insulation layer positioned over the conductive layer; another conductive layer positioned over the insulation layer that includes positioning electrodes; and another insulation layer positioned over the other conductive layer. The recording and positioning electrodes are electrically independent so as to independently receive a stimulus signal at each recording electrode and positioning electrode and independently detect a sensed signal at each recording electrode.

Abstract translation: 公开了用于记录电生理信号的技术，系统和装置。 在一个方面，微电极感测装置包括印刷电路板（PCB），与PCB电连接的芯片单元和位于芯片单元上方并且密封到PCB的细胞培养室，芯片单元位于PCB和PCB之间 细胞培养室。 芯片单元包括基板; 位于所述基板上方的包括一个或多个记录电极的导电层; 位于导电层上的绝缘层; 位于包括定位电极的绝缘层之上的另一个导电层; 以及位于另一导电层上的另一绝缘层。 记录和定位电极是电独立的，以便在每个记录电极和定位电极处独立地接收刺激信号，并独立地检测每个记录电极处的感测信号。

公开(公告)号：US07615758B2

公开(公告)日：2009-11-10

申请号：US11795660

申请日：2005-06-02

Applicant: Xianhua Wang ,  Hui Xu ,  Jianxin Ye ,  Yelei Sun ,  Guoliang Huang ,  Jing Cheng

Inventor： Xianhua Wang ,  Hui Xu ,  Jianxin Ye ,  Yelei Sun ,  Guoliang Huang ,  Jing Cheng

IPC: F21V9/16

CPC classification number: G01N21/6458 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/6421 ,  G01N2201/10 ,  G01N2201/103

Abstract: The invention provides a laser microarray scanner for microarray scanning, comprising an optical system, a scanning platform, and a data processing system. During scanning, the optical system remains fixed, and the microarray placed on the scanning platform moves relative to the optical system. The microarray scanner disclosed herein has high scanning speed, high sensitivity, high resolution, and high signal-to-noise ratio, thus is ideal for use in microarray scanning

Abstract translation: 本发明提供一种用于微阵列扫描的激光微阵列扫描器，包括光学系统，扫描平台和数据处理系统。 在扫描期间，光学系统保持固定，并且放置在扫描平台上的微阵列相对于光学系统移动。 本文公开的微阵列扫描器具有高扫描速度，高灵敏度，高分辨率和高信噪比，因此是理想的用于微阵列扫描

公开(公告)号：US20090251155A1

公开(公告)日：2009-10-08

申请号：US12288971

申请日：2008-10-24

Applicant: Lei Wang ,  Jing Zhu ,  Cheng Deng ,  Jing Cheng

Inventor： Lei Wang ,  Jing Zhu ,  Cheng Deng ,  Jing Cheng

IPC: G01R27/00

CPC classification number: G01N33/5029 ,  B01L3/502761 ,  B01L2200/10 ,  B01L2200/12 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/069 ,  B01L2300/0816 ,  B01L2300/16 ,  G01N33/5438

Abstract: Techniques, systems and apparatus are disclosed for detecting impedance. In one aspect, a microelectrode sensing device includes a substrate and an array of microelectrode sensors formed on the substrate. Each sensor includes at least one conductive layer formed above the substrate and patterned to include a counter electrode and multiple sensing electrodes to detect an electrical signal in absence and presence of one or more target cells positioned on at least a portion of a surface of each sensing electrode. The sensing electrodes are spaced apart and arranged around the counter electrode to provide a spatially averaged value of the detected electrical signal.

Abstract translation: 公开了用于检测阻抗的技术，系统和装置。 一方面，微电极感测装置包括基板和形成在基板上的微电极传感器阵列。 每个传感器包括形成在衬底上的至少一个导电层，并被图案化以包括对电极和多个感测电极，以在不存在和存在位于每个感测的表面的至少一部分上的一个或多个靶单元的情况下检测电信号 电极。 感测电极间隔开并布置在对电极周围，以提供检测到的电信号的空间平均值。

公开(公告)号：US20090101805A1

公开(公告)日：2009-04-23

申请号：US11795652

申请日：2005-06-02

Applicant: Xianhua Wang ,  Hui Zhu ,  Guoliang Huang ,  Jing Cheng

Inventor： Xianhua Wang ,  Hui Zhu ,  Guoliang Huang ,  Jing Cheng

IPC: H01J40/14

CPC classification number: G01N35/04 ,  G01N35/028

Abstract: The invention provides a scanning platform for high speed scanning of microarrays. The platform uses a novel flexible a metal strip/wheel linear driving system to convert rotary movement of motors into linear movement, thereby drives movement of a stage/microarray in the direction of scanning. The platform of the present invention provides high movement speed, high resolution, and low return deviation. It is also simple in structure and low in manufacturing cost.

Abstract translation: 本发明提供了用于微阵列高速扫描的扫描平台。 该平台使用新颖的柔性金属条/轮线性驱动系统将马达的旋转运动转换为直线运动，从而驱动载物台/微阵列沿扫描方向的移动。 本发明的平台提供高移动速度，高分辨率和低返回偏差。 结构简单，制造成本低。

公开(公告)号：US20080267828A1

公开(公告)日：2008-10-30

申请号：US11666032

申请日：2004-11-22

Applicant: Dong Wang ,  Kun Zou ,  Jianxing Ye ,  Yelei Sun ,  Hui Zhu ,  Xianhua Wang ,  Jing Cheng

Inventor： Dong Wang ,  Kun Zou ,  Jianxing Ye ,  Yelei Sun ,  Hui Zhu ,  Xianhua Wang ,  Jing Cheng

IPC: B65D83/00

CPC classification number: B01L3/0265 ,  B01L2400/0487 ,  B01L2400/0666 ,  Y10T436/2575

Abstract: The present invention relates to a micro-volume liquid ejection system, including an air pressure module, a micro-ejection unit which is connected with the air pressure module by means of pipes, and a control circuit which is connected with the air pressure module and the micro-ejection unit respectively. In the present invention, due to air is used as the pressure medium, on one hand, as the sample does not contact with the pressure regulating module, the efficiency of cleaning process is improved, on the other hand, the volume of the sample needed in the sample ejecting process is only equal to the cavity-dimension of the micro-ejection unit by not need to fill the whole pipe with liquid. During the sample ejecting process, it is not need to regulate the pressure. After the sample ejecting is finished, the sample can be put into its original place, so as to greatly save the sample. The present invention is connected with the manipulator, so as to sample from the sample plate, eject sample and clean the pipes automatically, and it can also dispense a multiplicity of samples to micro-array substrate conveniently. The present invention can be used for transferring or dispensing the micro volume liquid of nL grade and L grade widely, and all kinds of the micro-volume liquid including biological liquid.

Abstract translation: 微体积液体喷射系统技术领域本发明涉及一种微体积液体喷射系统，包括空气压力模块，通过管道与空气压力模块连接的微型喷射单元，以及与气压模块连接的控制电路和 微型喷射单元。 在本发明中，由于空气被用作压力介质，一方面由于样品不与压力调节模块接触，因此提高了清洗过程的效率，另一方面，样品的体积需要 在样品喷射过程中，仅通过不需要用整个管道填充液体就等于微喷射单元的腔体尺寸。 在样品喷射过程中，不需要调节压力。 样品喷射完成后，样品可以放在原来的位置，大大节省样品。 本发明与机械手连接，从样品板上取样，自动排出样品并清洗管道，方便地将多个样品分配给微阵列基板。 本发明可广泛用于转移或分配nL级和L级微量液体，以及各种微体积液体，包括生物液体。

公开(公告)号：US20070160984A1

公开(公告)日：2007-07-12

申请号：US11714954

申请日：2007-03-06

Applicant: Mingxian Huang ,  Lei Wu ,  Xiaobo Wang ,  Junquan Xu ,  Guo Tao ,  Jing Cheng

Inventor： Mingxian Huang ,  Lei Wu ,  Xiaobo Wang ,  Junquan Xu ,  Guo Tao ,  Jing Cheng

IPC: C12Q1/70 ,  G01N33/567 ,  B32B5/16 ,  G01N33/551

CPC classification number: C40B70/00 ,  B01J19/0046 ,  B01J2219/00459 ,  B01J2219/005 ,  B01J2219/00545 ,  B01J2219/00551 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00655 ,  B01J2219/0072 ,  B01J2219/0074 ,  B03C1/02 ,  B03C5/026 ,  B03C5/028 ,  B82Y15/00 ,  C40B40/06 ,  C40B50/16 ,  G01N33/54326 ,  G01N33/588 ,  Y10S428/90 ,  Y10T428/2982 ,  Y10T428/2991 ,  Y10T428/32

Abstract: This invention relates generally to the field of moiety or molecule isolation, detection and manipulation and library synthesis. In particular, the invention provides a bead, which bead comprises: a) a magnetizable substance; and b) an electrically conductive substance or an optical labeling substance. Methods and kits for isolating, detecting and manipulating moieties and synthesizing libraries using the beads are also provided.

Abstract translation: 本发明一般涉及部分或分子分离，检测和操作以及文库合成领域。 特别地，本发明提供了一种珠，该珠包括：a）可磁化物质; 和b）导电物质或光学标记物质。 还提供了用于分离，检测和操作部分并使用珠的合成文库的方法和试剂盒。

公开(公告)号：US07226860B2

公开(公告)日：2007-06-05

申请号：US10833154

申请日：2004-04-28

Applicant: Hsien-Ming Lee ,  Jing-Cheng Lin ,  Shing-Chyang Pan ,  Ming-Hsing Tsai ,  Hung-Wen Su ,  Shih-Wei Chou ,  Shau-Lin Shue ,  Kuo-Wei Cheng ,  Ting-Chu Ko

Inventor： Hsien-Ming Lee ,  Jing-Cheng Lin ,  Shing-Chyang Pan ,  Ming-Hsing Tsai ,  Hung-Wen Su ,  Shih-Wei Chou ,  Shau-Lin Shue ,  Kuo-Wei Cheng ,  Ting-Chu Ko

IPC: H01L21/44

CPC classification number: H01L21/2885 ,  H01L21/76843 ,  H01L21/76861 ,  H01L21/76862 ,  H01L21/76873 ,  H01L21/76877

Abstract: A method of electrochemical deposition (ECD) provides a barrier and a seed layer on a substrate. The surfaces of the substrate are pre-treated before a metal layer is electrochemically deposited thereon in an electrochemical plating cell with a physical or a chemical surface treatment process. The electrochemical plating cell is covered by a cap to prevent evaporation of the electrolyte solution. The electrochemical plating cell includes a substrate holder assembly with a lift seal, e.g., with a contact angle θ less than 90° between the lift seal and the substrate. The substrate holder assembly includes a substrate chuck at the rear side of the substrate.

公开(公告)号：US07215024B2

公开(公告)日：2007-05-08

申请号：US10936922

申请日：2004-09-08

Applicant: Jing-Cheng Lin ,  Cheng-Lin Huang ,  Ching-Hua Hsieh ,  Shau-Lin Shue ,  Mong-Song Liang

Inventor： Jing-Cheng Lin ,  Cheng-Lin Huang ,  Ching-Hua Hsieh ,  Shau-Lin Shue ,  Mong-Song Liang

IPC: H01L23/48

CPC classification number: H01L21/76843 ,  H01L21/32051 ,  H01L21/32136 ,  H01L21/76844 ,  H01L21/76846 ,  H01L21/76855 ,  H01L21/76877 ,  H01L21/76888 ,  H01L23/53238 ,  H01L23/53295 ,  H01L2924/0002 ,  H01L2924/00

Abstract: A new method is provided for the creation of a barrier-free copper interconnect. A dual damascene structure is created in a layer of dielectric, a thin metal barrier layer is deposited. The metal barrier layer is oxidized, two layers are then deposited with the first layer comprising doped copper and the second layer comprising pure copper. The dual damascene structure is filled with copper, a thermal anneal is applied, stabilizing the deposited copper filling the dual damascene structure and forming metal oxide of the doped minority element. Excess copper is then removed from the dielectric.

Abstract translation: 提供了一种创建无障碍铜互连的新方法。 在电介质层中形成双镶嵌结构，沉积薄金属阻挡层。 金属阻挡层被氧化，然后沉积两层，第一层包含掺杂的铜，第二层包含纯铜。 双镶嵌结构填充铜，进行热退火，稳定沉积的铜填充双镶嵌结构并形成掺杂少数元素的金属氧化物。 然后从电介质中除去过量的铜。