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公开(公告)号：US20140014517A1
公开(公告)日：2014-01-16
申请号：US14027941
申请日：2013-09-16
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vijay Srinivasan , Michael G. Pollack , Zhishan Hua , Arjun Sudarsan , Allen E. Eckhardt
IPC: G01N27/447
CPC classification number: G01N27/44743 , B01L3/0268 , B01L3/502761 , B01L3/502792 , B01L2200/0605 , B01L2200/061 , B01L2200/0647 , B01L2300/0645 , B01L2300/16 , B01L2400/0415 , B01L2400/0427
Abstract: Droplet actuator for conducting droplet operations, such as droplet transport and droplet dispensing, is provided. In one embodiment, the droplet actuator may include an electrode that is rotationally but not reflectively symmetrical.
Abstract translation: 提供用于进行液滴操作的液滴致动器，例如液滴传输和液滴分配。 在一个实施例中，液滴致动器可以包括旋转但不反射对称的电极。
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公开(公告)号：US20130252262A1
公开(公告)日：2013-09-26
申请号：US13890715
申请日：2013-05-09
Applicant: ADVANCED LIQUID LOGIC INC.
Inventor： Vijay Srinivasan , Vamsee K. Pamula , Michael G. Pollack , Richard B. Fair
IPC: G01N27/72
CPC classification number: G01N27/72 , G01N27/745 , G01N33/54326
Abstract: A method of detecting a target analyte in a sample, including executing electrowetting-mediated droplet operations and thereby: combining one or more immunoassay reagent droplets comprising magnetically-responsive beads having affinity for the target analyte with one or more sample droplets potentially comprising the target analyte to yield a first combined droplet; beginning with the combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a first washed droplet comprising the washed magnetically responsive beads; and combining the droplet comprising the washed magnetically responsive beads with a droplet comprising a reporter molecule having affinity for target analyte captured on the magnetically-responsive beads to yield a second combined droplet; beginning with the second combined droplet, effecting a droplet-based washing protocol to wash the magnetically-responsive beads to yield a second washed droplet comprising the washed magnetically responsive beads; detecting a signal from the second washed droplet which corresponds to the presence, absence and/or quantity of the analyte in the sample.
Abstract translation: 一种检测样品中的目标分析物的方法，包括执行电润湿介导的液滴操作，从而：将包含对靶分析物具有亲和性的磁响应珠粒的一种或多种免疫测定试剂液滴与潜在地包含靶分析物的一个或多个样品液 以产生第一组合液滴; 从组合的液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，以产生包含洗涤的磁响应珠的第一洗涤液滴; 以及将包含洗涤的磁响应珠粒的液滴与包含对在磁响应珠上捕获的靶分析物具有亲和力的报道分子的液滴组合以产生第二组合液滴; 从第二组合液滴开始，实现基于液滴的洗涤方案以洗涤磁响应珠，产生包含洗涤的磁响应珠的第二洗涤液滴; 检测来自第二洗涤液滴的信号，其对应于样品中分析物的存在，不存在和/或数量。
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公开(公告)号：US11865565B2
公开(公告)日：2024-01-09
申请号：US17962989
申请日：2022-10-10
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Uichong B. Yi , Vijay Srinivasan
CPC classification number: B05B5/0255 , B05B12/082 , G01F23/22 , G01F23/24 , G01F23/26
Abstract: Methods of improving accuracy of droplet metering using at least one on-actuator reservoir as the fluid input. In some embodiments, the on-actuator reservoir that is used for metering droplets includes a loading port, a liquid storage zone, a droplet metering zone, and a droplet dispensing zone. The on-actuator reservoirs are designed to prevent liquid flow-back into the loading port and to prevent liquid from flooding into the droplet operations gap in the dispensing zone.
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公开(公告)号：US11465161B2
公开(公告)日：2022-10-11
申请号：US16948238
申请日：2020-09-09
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Uichong B. Yi , Vijay Srinivasan
Abstract: The present invention is directed to methods of improving accuracy of droplet metering using at least one on-actuator reservoir as the fluid input. In some embodiments, the on-actuator reservoir that is used for metering droplets includes a loading port, a liquid storage zone, a droplet metering zone, and a droplet dispensing zone. The on-actuator reservoirs are designed to prevent liquid flow-back into the loading port and to prevent liquid from flooding into the droplet operations gap in the dispensing zone.
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公开(公告)号：US10139403B2
公开(公告)日：2018-11-27
申请号：US15266693
申请日：2016-09-15
Applicant: Advanced Liquid Logic, Inc.
Inventor： Vamsee K. Pamula , Ramakrishna Sista , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: B01L3/00 , G01N1/00 , G01N1/10 , G01N15/06 , G01N1/18 , G01N33/543 , B01F11/00 , B01F13/00 , G01N1/38 , B01F13/08 , B01L7/00 , G01N35/10
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
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Patent Agency Ranking
公开(公告)号：US09815061B2
公开(公告)日：2017-11-14
申请号：US14549113
申请日：2014-11-20
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Cyril Delattre , Arnaud Rival , Vijay Srinivasan
IPC: B01L3/00
CPC classification number: B01L3/502784 , B01L3/502715 , B01L3/502723 , B01L3/50273 , B01L3/5029 , B01L3/52 , B01L2200/06 , B01L2200/0605 , B01L2300/0645 , B01L2300/0816 , B01L2300/088 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0409 , B01L2400/0415 , B01L2400/0427 , B01L2400/043 , B01L2400/0442 , B01L2400/0448
Abstract: During droplet operations in a droplet actuator, bubbles often form in the filler fluid in the droplet operations gap and interrupt droplet operations. The present invention provides methods and systems for performing droplet operations on a droplet in a droplet actuator comprising maintaining substantially consistent contact between the droplet and an electrical ground while conducting multiple droplet operations on the droplet in the droplet operations gap and/or reducing the accumulation of electrical charges in the droplet operations gap during multiple droplet operations. The methods and systems reduce or eliminate bubble formation in the filler fluid of the droplet operations gap, thereby permitting completion of multiple droplet operations without interruption by bubble formation in the filler fluid in the droplet operations gap.
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公开(公告)号：US20160318021A1
公开(公告)日：2016-11-03
申请号：US15206860
申请日：2016-07-11
Applicant: Advanced Liquid Logic Inc.
Inventor： Vamsee K. Pamula , Michael G. Pollack , Vijay Srinivasan , Philip Paik
IPC: B01L3/00
CPC classification number: B01L3/502792 , B01L3/0241 , B01L3/50273 , B01L2200/06 , B01L2300/06 , B01L2400/0427 , B41J2/14
Abstract: A method of manipulating a droplet comprising providing a substrate comprising a surface; an elongated transport electrode disposed on the substrate surface, the elongated transport electrode having a first and a second end and configured to impart a gradient force to the droplet; and one or more wires for providing power to the transport electrode; and providing power to the one or more wires to effect the gradient force and thereby transport the droplet along the length of the elongated transport electrode from the first end to the second end.
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公开(公告)号：US09377455B2
公开(公告)日：2016-06-28
申请号：US14746276
申请日：2015-06-22
Applicant: Advanced Liquid Logic, Inc.
Inventor： Ramakrishna Sista , Vamsee K. Pamula , Vijay Srinivasan , Michael G. Pollack , Allen Eckhardt
IPC: G01N15/06 , G01N33/00 , G01N33/48 , G01N33/543 , B01F11/00 , B01F13/00 , B01L3/00 , G01N1/38 , B01L7/00 , G01N35/10
CPC classification number: G01N33/54326 , B01F11/0071 , B01F13/0071 , B01F13/0076 , B01F13/0809 , B01L3/50273 , B01L3/502761 , B01L3/502792 , B01L7/52 , B01L7/525 , B01L2200/027 , B01L2200/0647 , B01L2200/0673 , B01L2300/06 , B01L2300/0654 , B01L2300/0816 , B01L2300/0861 , B01L2300/0864 , B01L2300/0867 , B01L2300/089 , B01L2300/1827 , B01L2400/0406 , B01L2400/0415 , B01L2400/0421 , B01L2400/0424 , B01L2400/0427 , B01L2400/043 , B01L2400/0436 , B01L2400/0442 , G01N1/38 , G01N2001/386 , G01N2035/1046 , Y10T436/11 , Y10T436/25 , Y10T436/25625 , Y10T436/2575
Abstract: The invention provides a method of circulating magnetically responsive beads within a droplet in a droplet actuator. The invention also provides methods for splitting droplets. The invention, in one embodiment, makes use of a droplet actuator with top and bottom substrates, a plurality of magnetic fields respectively present proximate the top and bottom substrates, wherein at least one of the magnet fields is selectively alterable, and a plurality of droplet operations electrodes positioned along at least one of the top and bottom surfaces. A droplet is positioned between the top and bottom surfaces and at least one of the magnetic fields is selectively altered.
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公开(公告)号：US20160074863A1
公开(公告)日：2016-03-17
申请号：US14942125
申请日：2015-11-16
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Ryan A. Sturmer , Vijay Srinivasan , Arjun Sudarsan
CPC classification number: B01L3/50273 , B01L3/502792 , B01L2200/0621 , B01L2200/10 , B01L2200/14 , B01L2200/16 , B01L2300/0645 , B01L2300/0816 , B01L2300/0829 , B01L2300/087 , B01L2300/089 , B01L2400/0427 , G01N27/048 , G01R27/16
Abstract: Methods comprising measuring the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode are disclosed. Computer readable mediums storing processor executable instructions for performing the method, and systems are also disclosed. The systems comprise a processor, memory and code stored in the memory that when executed cause the processor at least to: receive an output voltage signal, superimpose an excitation signal onto the output voltage signal to produce a superimposed signal, connect the superimposed signal to an electrode in a droplet actuator, suppress the output voltage signal, when detecting an impedance of the electrode, and measure the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode.
Abstract translation: 方法包括测量由激发信号产生的电极的阻抗，其中阻抗表示在电极处存在液体。 还公开了存储用于执行该方法的处理器可执行指令的计算机可读介质，以及系统。 系统包括存储在存储器中的处理器，存储器和代码，当执行时，处理器至少要使接收输出电压信号，将激励信号叠加在输出电压信号上，产生叠加信号，将叠加的信号连接到 液滴致动器中的电极，当检测到电极的阻抗时抑制输出电压信号，并测量由激励信号产生的电极的阻抗，其中阻抗表示在电极处存在液体。
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60.发明申请公开(公告)号：US20150174578A1
公开(公告)日：2015-06-25
申请号：US14590470
申请日：2015-01-06
Applicant: ADVANCED LIQUID LOGIC, INC.
Inventor： Vijay Srinivasan , Michael G. Pollack , Vamsee K. Pamula , Zishan Hua , Arjun Sudarsan , Philip Y. Paik
IPC: B01L3/00
CPC classification number: B01L3/52 , B01F13/0071 , B01F13/0076 , B01L3/0241 , B01L3/502792 , B01L2200/027 , B01L2200/0605 , B01L2200/143 , B01L2200/16 , B01L2300/0816 , B01L2300/0819 , B01L2300/089 , B01L2400/0427 , B01L2400/06 , B01L2400/0688 , F04B19/006
Abstract: A droplet actuator with a droplet formation electrode configuration associated with a droplet operations surface, wherein the electrode configuration comprises one or more electrodes configured to control volume of a droplet during formation of a sub-droplet on the droplet operations surface. Methods of making and using the droplet actuator are also provided.
Abstract translation: 具有与液滴操作表面相关联的液滴形成电极配置的液滴致动器，其中所述电极配置包括一个或多个电极，其被配置为在液滴操作表面上形成子液滴期间控制液滴的体积。 还提供了制造和使用液滴致动器的方法。
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