公开(公告)号：US20050250133A1

公开(公告)日：2005-11-10

申请号：US11070519

申请日：2005-03-01

申请人： David Ecker ,  Steven Hofstadler ,  Richard Griffey ,  Rangarajan Sampath ,  Kristin Lowery ,  Christian Massire

发明人： David Ecker ,  Steven Hofstadler ,  Richard Griffey ,  Rangarajan Sampath ,  Kristin Lowery ,  Christian Massire

IPC分类号： C12N5/02 ,  C12N15/11 ,  C12Q1/68 ,  G01N33/48 ,  G01N33/50 ,  G06F19/00

CPC分类号： C12N15/11

摘要： Polynucleotides comprising molecular interaction sites of 16S rRNA that have particular secondary structure are provided. Methods of using such polynucleotides to screen, virtually or actually, combinatorial libraries of compounds that bind thereto are also provided. Method of modulating the activity of 16S rRNA by contacting 16S rRNA or prokaryotic cells containing the same with a compound identified by such virtual or actual screening are also provided.

摘要翻译： 提供了包含具有特定二级结构的16S rRNA的分子相互作用位点的多核苷酸。 还提供了使用这样的多核苷酸筛选，实际上或实际上筛选结合其的化合物的组合文库的方法。 还提供了通过将含有该酶的16S rRNA或原核细胞与通过这种虚拟或实际筛选鉴定的化合物接触来调节16S rRNA活性的方法。

公开(公告)号：US20050142581A1

公开(公告)日：2005-06-30

申请号：US10934798

申请日：2004-09-03

申请人： Richard Griffey ,  C. Bennett ,  David Ecker ,  Donna Ward ,  Susan Freier

发明人： Richard Griffey ,  C. Bennett ,  David Ecker ,  Donna Ward ,  Susan Freier

IPC分类号： C12N20060101 ,  C12N15/11 ,  C12N15/113 ,  C12Q1/68

CPC分类号： C12N15/111 ,  C12N15/1138 ,  C12N2310/11 ,  C12N2310/14 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/3341 ,  C12N2310/341 ,  C12N2310/346 ,  C12N2320/11 ,  C12N2330/10 ,  C12N2310/3525

摘要： The present invention provides methods for the identification of target molecules that bind to ligands, particularly microRNA ligands and mimics thereof and/or microRNA target molecules and mimics thereof, with as little as millimolar (mM) affinity using mass spectrometry. The methods may be used to determine the mode of binding interaction between two or more of these target molecules to the ligand as well as their relative affinities. Also provided are methods for designing compounds having greater affinity to a ligand by identifying two or more target molecules using mass spectrometry methods of the invention and linking the target molecules together to form a novel compound.

摘要翻译： 本发明提供用于使用质谱法以少至毫摩尔（mM）亲和力鉴定结合配体，特别是微小RNA配体及其模拟物和/或微小RNA靶分子及其模拟物的靶分子的方法。 该方法可用于确定这些靶分子中两个或更多个与配体之间的结合相互作用的模式以及它们的相对亲和力。 还提供了通过使用本发明的质谱方法鉴定两个或更多个靶分子并将靶分子连接在一起以形成新化合物来设计对配体具有更大亲和力的化合物的方法。

公开(公告)号：US06656690B2

公开(公告)日：2003-12-02

申请号：US09884317

申请日：2001-06-19

申请人： Stanley T. Crooke ,  Richard Griffey ,  Steven Hofstadler

发明人： Stanley T. Crooke ,  Richard Griffey ,  Steven Hofstadler

IPC分类号： C12Q168

CPC分类号： C12Q1/6816 ,  B01J2219/00702 ,  B01J2219/00722 ,  C12Q1/6806 ,  C12Q1/6811 ,  C40B40/06 ,  H01J49/004 ,  Y10T436/24 ,  C12Q2565/627 ,  C12Q2525/101 ,  C12Q2565/133 ,  C12Q2525/121 ,  C12Q2565/607

摘要： The present invention provides methods for the determination of the structure of biomolecular targets, as well as the site and nature of the interaction between ligands and biomolecular targets. The present invention also provides methods for the determination of the relative affinity of a ligand for the biomolecular target it interacts with. Also provided are methods for screening ligand or combinatorial libraries of compounds against one or more than one biological target molecules. The methods of the invention also allow determination of the relative binding affinity of combinatorial and other compounds for a biomolecular target. The present invention further provides methods for the use of mass modifying tags for screening multiple biomolecular targets. In a preferred embodiment, ligands which have great specificity and affinity for molecular interaction sites on biomolecules, especially RNA can be identified. In preferred embodiments, such identification can be made simultaneously with libraries of ligands.

公开(公告)号：US06358931B1

公开(公告)日：2002-03-19

申请号：US08295744

申请日：1994-08-30

申请人： Phillip Dan Cook ,  Thomas Bruice ,  Charles John Guinosso ,  Andrew Mamoru Kawasaki ,  Richard Griffey

发明人： Phillip Dan Cook ,  Thomas Bruice ,  Charles John Guinosso ,  Andrew Mamoru Kawasaki ,  Richard Griffey

IPC分类号： C07H2107

CPC分类号： C12N15/113 ,  A61K38/00 ,  A61K48/00 ,  C07H19/04 ,  C07H19/06 ,  C07H19/10 ,  C07H19/16 ,  C07H19/20 ,  C07H21/00 ,  C07H23/00 ,  C07J43/003 ,  C07K14/005 ,  C12N9/0069 ,  C12N15/1137 ,  C12N2310/3125 ,  C12N2310/315 ,  C12N2310/32 ,  C12N2310/321 ,  C12N2310/322 ,  C12N2310/33 ,  C12N2310/333 ,  C12N2310/336 ,  C12N2310/3511 ,  C12N2310/3521 ,  C12N2310/3523 ,  C12N2310/3527 ,  C12N2310/3531 ,  C12N2310/3533 ,  C12N2740/16322 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Y113/11012

摘要： Compositions and methods for modulating the activity of RNA are disclosed, In accordance with preferred embodiments, antisense compositions are prepared targeting reactive portions. The reactive portions preferably comprise one or two imidazole functionalities conjugated to the targeting oligonucleotide via linkers with or without intervening intercalating moieties. Therapeutics, diagnostics and research methods also are disclosed, as are synthetic nucleosides and nucleoside fragments that can be elaborated into oligonucleotides.

摘要翻译： 公开了调节RNA活性的组合物和方法。根据优选实施方案，制备靶向反应部分的反义组合物。 反应性部分优选包含通过具有或不具有插入插入部分的接头与靶向寡核苷酸缀合的一个或两个咪唑官能团。 还公开了治疗学，诊断和研究方法，合成核苷和可以被制成寡核苷酸的核苷片段也是公开的。

公开(公告)号：US06221587B1

公开(公告)日：2001-04-24

申请号：US09076440

申请日：1998-05-12

申请人： David J. Ecker ,  Ranga Sampath ,  Richard Griffey ,  John McNeil

发明人： David J. Ecker ,  Ranga Sampath ,  Richard Griffey ,  John McNeil

IPC分类号： C12Q168

CPC分类号： C12Q1/6809

摘要： Methods of identifying molecular interaction sites in eukaryotic and prokaryotic nucleic acids, especially RNA, are described. Secondary structural elements are identified from highly conserved sequences. Methods of preparing databases relating to such molecular interaction sites are also provided herein as are databases themselves. Therapeutic, agricultural, industrial, and other applicability results from interaction of such molecular interaction sites with “small” and other molecules.

摘要翻译： 描述了在真核和原核核酸，特别是RNA中鉴定分子相互作用位点的方法。 二级结构元件由高度保守的序列鉴定。 本文还提供了与这种分子相互作用位点相关的数据库的制备方法，数据库本身也是如此。 这种分子相互作用位点与“小”和其他分子的相互作用导致治疗，农业，工业和其他适用性。

公开(公告)号：US5874213A

公开(公告)日：1999-02-23

申请号：US469852

申请日：1995-06-06

申请人： Lendell L. Cummins ,  Susan M. Freier ,  Richard Griffey ,  G. Susan Srivatsa

发明人： Lendell L. Cummins ,  Susan M. Freier ,  Richard Griffey ,  G. Susan Srivatsa

IPC分类号： C12Q1/68 ,  G01N27/447

CPC分类号： G01N27/44747 ,  C12Q1/68 ,  C12Q1/6813

摘要： Methods are provided for detection and quantitation of mixtures containing target nucleobase sequences using capillary electrophoresis. Peptide nucleic acid oligomers, complementary to the target sequences and preferably having appended detectable labels, are hybridized to the targets. Capillary electrophoresis is then performed, and the detectable label is detected and quantitated.

公开(公告)号：US20120156283A1

公开(公告)日：2012-06-21

申请号：US13332738

申请日：2011-12-21

申请人： John Dresios ,  Richard Griffey

发明人： John Dresios ,  Richard Griffey

IPC分类号： A61K39/395 ,  A61K9/127 ,  A61P17/02 ,  G01N33/68

CPC分类号： G01N33/6887 ,  A61K9/1271 ,  A61K38/02 ,  A61K38/1777 ,  A61K39/44 ,  A61K47/42 ,  A61K47/6801 ,  A61K47/6803 ,  A61K47/6811 ,  A61K47/6847 ,  A61K49/0058 ,  C07K16/18 ,  G01N33/5088 ,  G01N33/567 ,  G01N33/6893 ,  G01N2333/47 ,  G01N2333/4712 ,  G01N2333/78

摘要： The present disclosure relates to methods for identifying proteins or peptide motifs of intracellular, extracellular, or extracellular matrix proteins specifically exposed in wound sites, as well as compositions for treating wounds, and methods for their use.

摘要翻译： 本公开涉及用于鉴定特异性暴露于伤口部位的细胞内，细胞外或细胞外基质蛋白以及用于治疗伤口的组合物的蛋白质或肽基序的方法及其使用方法。

公开(公告)号：US20050239737A1

公开(公告)日：2005-10-27

申请号：US11146468

申请日：2005-06-07

申请人： David Ecker ,  Ranga Sampath ,  Richard Griffey ,  John McNeil

发明人： David Ecker ,  Ranga Sampath ,  Richard Griffey ,  John McNeil

IPC分类号： C12N15/09 ,  A61K31/7105 ,  A61K31/713 ,  A61K48/00 ,  A61P31/04 ,  C07H21/02 ,  C07H21/04 ,  C12N15/11 ,  C12Q1/68

CPC分类号： C12Q1/6876

摘要： Methods of identifying molecular interaction sites in eukaryotic and prokaryotic nucleic acids, especially RNA, are described. Secondary structural elements are identified from highly conserved sequences. Methods of preparing databases relating to such molecular interaction sites are also provided herein as are databases themselves. Therapeutic, agricultural, industrial, and other applicability results from interaction of such molecular interaction sites with “small” and other molecules.

摘要翻译： 描述了在真核和原核核酸，特别是RNA中鉴定分子相互作用位点的方法。 二级结构元件由高度保守的序列鉴定。 本文还提供了与这种分子相互作用位点相关的数据库的制备方法，数据库本身也是如此。 这种分子相互作用位点与“小”和其他分子的相互作用导致治疗，农业，工业和其他适用性。

公开(公告)号：US20050123952A1

公开(公告)日：2005-06-09

申请号：US10933928

申请日：2004-09-03

申请人： Richard Griffey ,  David Ecker

发明人： Richard Griffey ,  David Ecker

IPC分类号： C12N20060101 ,  C12P19/34 ,  C12Q1/68

CPC分类号： C12Q1/6888 ,  C12Q1/6816 ,  C12Q2600/156 ,  C12Q2531/113 ,  C12Q2525/15 ,  C12Q2565/627

摘要： Methods for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of microRNA containing nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of microRNA containing nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) or molecular mass which is then matched against a database of base composition signatures or molecular masses, by which the species of the bioagent is identified.

摘要翻译： 通过使用与含有衍生自生物制剂的含有核酸的微RNA的保守序列区杂交的引物与包含核酸扩增的微小RNA的组合来检测和识别未知生物标签（包括细菌，病毒等）的方法， 唯一识别生物标签的序列区域。 结果是“基础组成特征”（BCS）或分子量，然后与基础组成特征或分子量的数据库进行匹配，通过该数据库识别出生物代谢物种。

公开(公告)号：US20050029447A1

公开(公告)日：2005-02-10

申请号：US10939753

申请日：2004-09-13

申请人： Steven Hofstadler ,  Jared Drader ,  Kristin Sannes-Lowery ,  Richard Griffey

发明人： Steven Hofstadler ,  Jared Drader ,  Kristin Sannes-Lowery ,  Richard Griffey

IPC分类号： H01J49/04 ,  H01J49/00 ,  B01D59/44

CPC分类号： H01J49/04

摘要： The present disclosure is related to improved systems and methods for delivering samples for high-throughput mass spectrometric analysis to an atmospheric-pressure ionization source. In an exemplary embodiment, the system includes a solvent reservoir for storing a solvent solution, a first valve which is coupled to the solvent reservoir, first and second pumps for delivering solvent solution and which are coupled to the first valve and which the delivery flow rate of the first pump is greater than the delivery flow rate of the second pump, an injection system having a sample injector and an second valve which is coupled to the first valve and which is capable of being coupled to can be couple to an electrospray ionization source. In another embodiment, the system can also include an atmospheric-pressure ionization chamber, an atmospheric-pressure ionization sprayer and a nebulizer gas source and a voltage supply source. In yet another embodiment, the system may further include a puffer valve that is coupled to the nebulizer gas source and the atmospheric-pressure ionization sprayer and a gas puffer that is coupled to the puffer valve. A distal end of the gas puffer may be located within the atmospheric-pressure ionization chamber and aligned with the distal end of the atmospheric-pressure ionization sprayer and the puffer valve may control the delivery of the nebulizer gas to the atmospheric-pressure ionization sprayer and the gas puffer.

摘要翻译： 本公开涉及用于将用于高通量质谱分析的样品递送到大气压电离源的改进的系统和方法。 在示例性实施例中，系统包括用于存储溶剂溶液的溶剂储存器，耦合到溶剂储存器的第一阀，用于输送溶剂溶液的第一和第二泵，并且其耦合到第一阀，并且输送流量 的第一泵的输送流量大于第二泵的输送流量，具有样品注射器的注射系统和耦合到第一阀并且能够耦合的第二阀可以耦合到电喷雾离子源 。 在另一个实施例中，系统还可以包括大气压电离室，大气压电离喷雾器和喷雾器气体源和电压源。 在另一个实施例中，该系统还可以包括与喷雾器气体源和大气压电离喷雾器相连的吹气阀以及耦合到吹气阀的气体吹气器。 气体吹气器的远端可以位于大气压电离室内并与大气压电离喷雾器的远端对准，并且吹气阀可以控制喷雾器气体输送到大气压电离喷雾器， 气体吹气